Identification of proteins that interact with NF-YA
Abstract
We used the yeast two-hybrid system to show that the serum response factor (SRF) and zinc-fingers and homeobox 1 (ZHX1) proteins interact with the A subunit of nuclear factor-Y (NF-YA). GST pulldown assays revealed that both proteins interact specifically with NF-YA in vitro. Amino acids located between 272 and 564, a region that contains two homeodomains, are required for the interaction of ZHX1 with NF-YA. Two different domains of NF-YA, a glutamine-rich region and a serine/threonine-rich region, are necessary for the interactions with ZHX1 and SRF, respectively.
- Publication:
-
FEBS Letters
- Pub Date:
- January 1999
- DOI:
- Bibcode:
- 1999FEBSL.460...41Y
- Keywords:
-
- Transcription factor;
- Nuclear factor-Y;
- Serum response factor;
- Zinc-finger;
- Homeobox 1;
- Protein-protein interaction;
- NF-Y;
- nuclear factor-Y;
- NF-YA;
- A subunit of NF-Y;
- AD;
- activation domain;
- ZHX1;
- zinc-fingers and homeobox-1;
- SRF;
- serum response factor;
- Znf;
- zinc-fingers;
- HD;
- homeodomain;
- GST;
- glutathione S-transferase;
- PCR;
- polymerase chain reaction;
- DBD;
- DNA binding domain;
- SID;
- subunit interaction domain;
- SD-His;
- histidine-free synthetic dextrose;
- HNF4;
- hepatocyte nuclear factor 4