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. 2019 Oct 21:27:104704.
doi: 10.1016/j.dib.2019.104704. eCollection 2019 Dec.

A dataset on the effect of exercise-conditioned human sera in three-dimensional breast cancer cell culture

Mauro De Santi  1 , Giulia Baldelli  1 , Francesco Lucertini  2 , Valentina Natalucci  2 , Giorgio Brandi  1 , Elena Barbieri  2   3
Affiliations

A dataset on the effect of exercise-conditioned human sera in three-dimensional breast cancer cell culture

Mauro De Santi et al. Data Brief. .

Abstract

Epidemiological evidence shows that physical activity lowers the risk of developing breast cancer and decreases the risk of disease recurrence [1,2]. The main hypothesis on the positive effects of exercise-oncology has focused on lowering the basal systemic levels of cancer risk factors with exercise training. Recently, the effects of cancer progression control by components released after acute exercise bouts have gained attention [3,4]. However, the evaluation of the antiproliferative potential of a single exercise bout needs technical improvement. Here, we present data of a pilot study showing how to evaluate the anti-cancer potential of single exercise bouts with an in vitro three-dimensional cell growth assay, using a triple-negative breast cancer cell line cultured with exercise-conditioned serum.

Keywords: Cell proliferation; Exercise; Three-dimensional in vitro culture; Triple negative breast cancer.

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Figures

Fig. 1
Fig. 1
Details of the acute exercise interventions. The time schedule of pre- (t0) and post-exercise (t1; t2) sampling is shown in the timeline. Blood samples for in vitro assays were drawn according to protocol exercise at t0, t1 and t2.//, rest period; HRR, heart rate reserve.
Fig. 2
Fig. 2
MTS assay. Evaluation of cell viability after 72 hours of incubation with 5% of pre- or post-exercise sera and with culture medium supplemented with 0.8 or 1.2 mg/mL of glucose. t0: pre-exercise serum; t1: immediately after exercise serum; t3: 2 hours post-exercise serum. s-01, subject 01; s-02, subject 02; s-03, subject 03. Data are expressed as mean ± SEM of five experiments; *p < 0.05.
Fig. 3
Fig. 3
Soft-agar assay. Evaluation of MDA-MB-231 colony formation after 18 days of incubation with 5% of pre- or post-exercise human sera. s-01, subject 01; s-02, subject 02; s-03, subject 03. Data are expressed as mean ± SEM of four experiments; **p < 0.01, ***p < 0.001, respect to t0.
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