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. 2008 Mar;3(1):52-6.
doi: 10.1007/s11481-007-9091-1. Epub 2007 Nov 29.

Cocaine-mediated alteration in tight junction protein expression and modulation of CCL2/CCR2 axis across the blood-brain barrier: implications for HIV-dementia

Navneet K Dhillon  1 , Fuwang Peng, Sirosh Bokhari, Shannon Callen, Sun-Hye Shin, Xuhui Zhu, Kee-Jun Kim, Shilpa J Buch
Affiliations

Cocaine-mediated alteration in tight junction protein expression and modulation of CCL2/CCR2 axis across the blood-brain barrier: implications for HIV-dementia

Navneet K Dhillon et al. J Neuroimmune Pharmacol. 2008 Mar.

Abstract

One of the hallmark features underlying the pathogenesis of HIV encephalitis is the disruption of blood-brain barrier (BBB). Cocaine, often abused by HIV-infected patients, has been suggested to worsen the HIV-associated dementia (HAD) via unknown mechanisms. The objective of the present study was to explore the effects of cocaine on BBB permeability using human brain microvascular endothelial cells (HBMECs). Additionally, because the chemokine CCL2 and its receptor CCR2 play a crucial role in the recruitment of inflammatory cells into the central nervous system in HAD brains, we tested for the effect of cocaine in modulating the CCL2/CCR2 axis. Our findings suggest that exposure of HBMECs to cocaine correlated with the breakdown of ZO-1 tight junction protein and reorganization of the cytoskeleton resulting in stress fiber formation. Furthermore, cocaine also modulated upregulation of the CCL2/CCR2 axis in monocytes. These findings conform to the multifaceted effects of cocaine leading to accelerated progression of HIV-1 neuropathogenesis.

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References

    1. Proc Natl Acad Sci U S A. 1998 Mar 17;95(6):3117-21 - PubMed
    1. Am J Physiol Cell Physiol. 2004 Jan;286(1):C31-42 - PubMed
    1. J Neuroimmunol. 1998 Mar 15;83(1-2):133-8 - PubMed
    1. J Acquir Immune Defic Syndr. 2002 Oct 1;31 Suppl 2:S62-9 - PubMed
    1. J Cereb Blood Flow Metab. 2005 May;25(5):593-606 - PubMed

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