TWI626445B - Detecting device - Google Patents

Abstract

The invention provides a detecting device, which mainly comprises a sample liquid carrying turntable, a micro flow channel structure, an electromagnetic guiding module and an optical analysis module, wherein the micro flow channel structure is used for accommodating the sample liquid and the detecting liquid, And the solid reaction of the antibody of the sample liquid with the antigen of the detection liquid to the reaction tank of the microchannel structure by the rotation of the sample liquid carrying disk; further, the antibody of the invention is combined with the metal label and further penetrates The metal label and the electromagnetic guiding module can achieve the effect of guiding the antibody and the antigen to the reaction tank, and at the same time, the detection technology of the present invention can be detected by optical analysis through the metal label.

Description

Testing device

The present invention relates to antibody detection technology, and more particularly to a detection device which can increase the detection speed of an antibody.

Biochemical testing, especially enzyme-linked immunosorbent assay (ELISA), is a rapid detection method. Because of its high specificity, rapidity, sensitivity, low cost of testing, and the ability to perform large sample tests at the same time, ELISA Therefore, it has a wide range of practical applications. The principle of ELISA is the specific binding between antigen and antibody, which can be used to detect and preliminarily quantify specific antigens or antibodies.

Among them, sandwich ELISA is used as an example. In the experiment, it is necessary to add a capture antibody, an antigen, a detection antibody labeled with HRP, and Color liquid. Each step requires an incubation time and a wash procedure. Therefore, the entire enzyme sputum immunosorbent method requires a relatively long execution time (數 hours to days) and complicated execution procedures. Most of the existing implementations are performed using a microtiter plate, but this method requires manual injection of the sample and reagent into multiple reaction tanks in a manual manner, which makes the operation very labor intensive and time consuming.

In order to improve the problems caused by the above microtiter plate, a technique for performing ELISA detection on a microfluidic disk platform has been developed, which is called "CD-ELISA". In this technique, the transfer tester only needs to inject the reagent into each storage tank on the test turntable in advance, and the reagent release and mixing reaction can be automatically performed to complete the ELISA procedure.

Although the above-mentioned CD-ELISA technique can improve the execution procedure of the conventional technology, since it is still necessary to perform the detection by the mutual reaction of the enzyme and the coloring liquid, it is still necessary to perform a considerable process in the enzyme chelation immunosorption process. Long execution time.

Through the above, it can be known that the conventional antibody detecting technique still has disadvantages and shortcomings; in view of this, the inventors of the present invention have vigorously studied the invention and finally developed a detecting device of the present invention.

The invention provides a detecting device, which mainly comprises a sample liquid carrying turntable, a micro flow channel structure, an electromagnetic guiding module and an optical analysis module, wherein the micro flow channel structure is used for accommodating the sample liquid and the detecting liquid, And the solid reaction of the antibody of the sample liquid with the antigen of the detection liquid to the reaction tank of the microchannel structure by the rotation of the sample liquid carrying disk; further, the antibody of the invention is combined with the metal label and further penetrates The metal label and the electromagnetic guiding module can achieve the effect of guiding the antibody and the antigen to the reaction tank, and at the same time, the detection technology of the present invention can be detected by optical analysis through the metal label.

Therefore, in order to achieve the above object of the present invention, the inventors of the present invention have proposed a detecting apparatus comprising: a sample liquid carrying turntable; at least one micro flow path structure coupled to a reaction tank, and the micro flow path structure and the The reaction tank is formed on the sample liquid carrying turntable, and the micro flow channel structure is configured to respectively accommodate at least one sample liquid, at least one detecting liquid or a metal label or an eluent or any two of the foregoing a combination of the above; a motor connected to the sample liquid carrying turntable for driving the sample liquid carrying turntable to rotate, thereby providing a centrifugal force of the micro flow path structure; and a combination of any two or more of the following two or more modules The heating module comprises: a heating module corresponding to the reaction tank and heating a reaction liquid, wherein the reaction liquid passes through the microfluidic structure, the sample liquid, the detection liquid and the eluent flow in An electromagnetic guiding module corresponding to the microchannel structure and configured to provide a magnetic field or an electric field in or near the microchannel structure; and an optical The analysis module corresponds to the microchannel structure or the area within or near the reaction tank.

In order to more clearly describe a detecting device of the present invention, the preferred embodiments of the present invention will be described in detail below with reference to the drawings.

Please refer to FIG. 1 , FIG. 2 and FIG. 3 , which are respectively a schematic structural view of a detecting device of the present invention, a perspective view of a sample liquid carrying turntable of the present invention, and a perspective view of a micro flow channel structure. As shown in FIG. 1 , FIG. 2 and FIG. 3 , an embodiment of the present invention is an antibody detecting device 1 mainly comprising: a sample liquid carrying turntable 11 , a plurality of micro flow channel structures 12 , a motor 14 , and a heating die The group 15, an electromagnetic guiding module 16 and an optical analysis module 17.

Wherein, the sample liquid carrying turntable 11 is formed of a transparent material, and the micro flow channel structures 12 are formed in an array on the sample liquid carrying turntable 11, and each micro flow channel structure 12 is used for respectively accommodating A sample liquid 131, a detection liquid 132, and an eluent 133, and as shown in FIG. 3, the antibody 1311 of the sample liquid 131 is combined with at least one metal label 134. In addition, the motor 14 is connected to the sample liquid carrying turntable 11 for driving the sample liquid carrying turntable 11 to rotate, thereby providing a centrifugal force of the micro flow channel structure 12, wherein the centrifugal force can make the sample liquid 131 and the detecting liquid 132 and the eluent 133 flow into the reaction vessel 121 of one of the microchannel structures 12 to produce a reaction liquid 13.

Further, the heating module 15 corresponds to the reaction tank 121 and heats the reaction liquid 13, thereby increasing the solid phase reaction rate of the antibody 1311 of the sample liquid 131 and the antigen 1321 of the detection liquid 132, and the electromagnetic conduction The lead-in module 16 corresponds to the micro-channel structure 12 and is configured to provide a magnetic field or an electric field to the micro-channel structure 12, and then direct the antibody 1311 of the sample liquid 131 to the reaction by sensing the metal label 134. Slot 121.

In the above, it is worth noting that, in the technique of the present invention, the metal marker 134 is used to conjugate to the sample liquid 131 or the detection liquid 132. Further, the electromagnetic guiding module 16 is permeable. Two magnetization coils are respectively provided above and below the microchannel structure 12 to cause a magnetic field direction between the magnetization coils, thereby causing the magnetic field direction to attract or repel the metal marker 134 to the outward direction F. The antibody 1311 that guides the sample liquid 131 flows into the reaction tank 121. On the other hand, in the actual production, the electromagnetic guiding module 16 can also be used to provide the electric field of the micro-channel structure 12, and then push the metal marker 134 to the outward direction F through the potential energy or the micro-current. The antibody 1311 that guides the sample liquid 131 flows into the reaction tank 121.

As shown in FIG. 1, the optical analysis module 17 corresponds to the reaction tank 121 and analyzes the reaction liquid 13 and outputs antibody detection data to the external computer 2. In addition, the antibody detecting device 1 of the present invention further includes an optical positioning module 18 and an image capturing module 19; wherein the optical positioning module 18 corresponds to the microchannel structure 12 and is used to position the microchannel structure. The antibody 1311 and the antigen 1321 are disposed on the 12, and the optical positioning module 18 is further configured to position the rotation rate of the sample liquid carrying disk 11 . In addition, the image capturing module 19 corresponds to the micro-channel structure 12 for capturing an instant image of the micro-channel structure 12, wherein the image capturing module 19 is a high-speed CCD camera. ).

On the other hand, in the present embodiment, the metal marker 134 is a nano-gold-labeled particle, and in practical applications, the metal marker 134 may also be a nano-silver (Ag) particle or a nano-copper (Cu). Magnetic particles, such as microparticles, nano-iron (Fe) particles, nano-cobalt (Co) particles, and nano-nickel (Ni) particles, and the heating module 15 is an optical heating module and is used to provide a wave. The reaction liquid 13 is locally heated. Further, the optical analysis module 17 is a laser induced fluorescent (LIF) detector, and the metal marker 134 is induced to excite the fluorescent light. The reaction liquid 13 was detected and analyzed in a manner.

As shown in FIG. 2 and FIG. 3, the reaction tank 121 is composed of a plurality of microchannels 1211 arranged in parallel, and the microchannel structure 12 further includes: a first eluent injection tank 122, a sample injection tank 123, a detection liquid injection tank 124, a second eluent injection tank 126, and two waste liquid storage tanks 127; wherein the first eluent injection tank 122 and the second eluent are injected The tank 126 is for storing the eluent 133, and the sample injecting tank 123 is for storing the sample liquid 131, and the detecting liquid injecting tank 124 is for storing the detecting liquid 132.

It should be particularly noted that the reaction tank 121 is formed on the outermost edge of the sample liquid carrying turntable 11 with respect to the first eluent injection tank 122, the sample injection tank 123, and the detection liquid injection tank 124. And the reaction tank 121 extends toward the center of the sample liquid carrying disk 11 with a reaction tank injection flow path 125, and the first eluent injection tank 122, the sample injection tank 123, the detection liquid injection tank 124, and the The second eluent injection tank 126 is connected to the reaction tank injection flow path 125, respectively.

In addition, the two waste liquid storage tanks 127 are respectively disposed adjacent to the left and right sides of the reaction tank 121, and each waste liquid storage tank 127 is connected to the reaction tank 121 and the first eluent injection tank 122, respectively. The sample injecting tank 123, the detecting liquid injecting tank 124, and the second eluent injecting tank 126 are used to store the waste liquid in the antibody detecting process.

In the technique of the present invention, the detection liquid 132 may be any one of the following liquids: a virus detection liquid, a bacteria detection liquid, a drug detection liquid, an alcohol detection liquid, a cell detection liquid, and blood, etc., which can be used for an enzyme-linked adsorption reaction. The liquid is detected; and, in contrast, the sample liquid 131 is a relative sample liquid which can react with the above-mentioned detection liquid.

Thus, the above-mentioned system has clearly and completely explained the estimation principle and technical features of an antibody detecting device of the present invention; and, through the above, we can know that the present invention has the following advantages:

1. The fluorescence detection and metal label proposed by the present invention will improve and replace the traditional enzyme-linked immunosorbent assay (ELISA), and must wait for the time and cost of the enzyme coloring step. .

2. In the above first point, the electromagnetic guiding module of the present invention can further guide the metal marker, so that the mixed solution of the sample liquid, the detecting liquid and the eluent can smoothly flow into the reaction tank.

3. In addition, the reaction tank of the present invention is composed of multiple microchannels arranged in parallel, and thus, the surface area of the solid phase reaction between the antigen and the antibody can be effectively increased, thereby increasing the reaction rate.

It is to be understood that the foregoing detailed description of the embodiments of the present invention is not intended to Both should be included in the scope of the patent in this case.

<present invention>

1‧‧‧Antibody detection device

2‧‧‧ computer

11‧‧‧Sample liquid carrying turntable

12‧‧‧Microchannel structure

121‧‧‧Reaction tank

1211‧‧‧microchannel

122‧‧‧First eluent injection tank

123‧‧‧sample injection tank

124‧‧‧Detection solution injection tank

125‧‧‧Reaction tank injection flow path

126‧‧‧Second eluent injection tank

127‧‧‧ Waste storage tank

13‧‧‧Reaction solution

131‧‧‧ sample liquid

1311‧‧‧ antibody

132‧‧‧Detection fluid

1321‧‧ ‧ antigen

133‧‧‧ Eluent

134‧‧‧Metal markers

14‧‧‧Motor

15‧‧‧heating module

16‧‧‧Electromagnetic guidance module

17‧‧‧Optical Analysis Module

18‧‧‧Optical Positioning Module

19‧‧‧Image capture module

F‧‧‧ directions

BRIEF DESCRIPTION OF THE DRAWINGS Fig. 1 is a schematic view showing the structure of a detecting device of the present invention; Fig. 2 is a perspective view showing a sample carrying wheel of the present invention; and Fig. 3 is a perspective view showing the structure of a microchannel according to the present invention.

Claims (14)

A detecting device comprises at least: a sample liquid carrying turntable; a plurality of micro flow channel structures connected to a reaction tank, and the micro flow channel structure and the reaction tank system are formed on the sample liquid carrying turntable, and the The micro flow channel structure is configured to respectively accommodate at least one sample liquid, at least one detecting liquid and one eluent; a motor is connected to the sample liquid carrying turntable for driving the sample liquid carrying turntable to rotate, thereby providing the micro a flow channel structure-centrifugal force; a heating module for heating the micro-channel structure or the reaction tank; an electromagnetic guiding module corresponding to the micro-channel structure for providing a magnetic field or a An electric field in or near the microchannel structure; wherein at least one metal marker is used to bond to the sample liquid or the detection liquid, and the magnetic field or the electric field provided by the electromagnetic guiding module Orienting the sample liquid or the detection liquid to the reaction tank by attracting or repelling the metal marker to a specific direction; an optical analysis module corresponding to the micro flow channel structure or the reaction tank Or a nearby area; and an optical positioning module corresponding to the micro-channel structure and configured to locate the sample liquid or the detection liquid in the micro-channel structure; at the same time, the optical positioning module is further used for positioning The sample liquid carries the rate of rotation of the turntable. The detecting device according to claim 1, wherein the detecting liquid is used for reacting with the sample liquid, and the detecting liquid may be any one of the following: a virus detecting liquid, a bacteria detecting liquid, a drug detecting liquid, Alcohol test solution, cell test solution, or protein test solution. The detecting device of claim 1, further comprising an image capturing module corresponding to the microchannel structure for capturing an instant image of the microchannel structure, wherein the image capturing module The group is a High Speed CCD Camera. The detecting device of claim 1, wherein the heating module is an optical heating module for locally heating a reaction liquid, and the reaction liquid is passed through the sample liquid of the micro flow channel structure. The test solution and the eluent are introduced into the reaction tank to be produced. The detection device of claim 1, wherein the optical analysis module is a laser induced fluorescent (LIF) detector. The detection device of claim 5, wherein the laser-induced fluorescence detector detects and analyzes a reaction solution by inducing the metal marker to excite fluorescence, and the reaction solution The sample liquid, the detection liquid, and the eluent that have passed through the microchannel structure are generated by flowing into the reaction tank. The detecting device of claim 1, wherein the metal marking is used to bind to the sample liquid or the detecting liquid, and the magnetic field provided by the electromagnetic guiding module transmits or rejects the magnetic field. The metal marker is directed to a specific direction to direct the sample liquid to the reaction vessel. The detecting device of claim 1, wherein the number of the micro flow channel structures is plural, and the micro flow channel structures are arranged in an array on the sample liquid carrying turntable. The detecting device according to claim 1, wherein the metal label may be any one of the following magnetic metal particles: nano gold labeled particles, nano silver particles, nano copper particles, nano iron particles, nai Rice cobalt particles, or nano nickel particles. The detecting device according to claim 1, wherein the reaction tank is composed of a plurality of microchannels arranged in parallel. The detecting device of claim 10, wherein the microchannel structure comprises: a first eluent injection tank for storing the eluent; and a sample injection tank for storing The sample liquid; and a detection liquid injection tank for storing the detection liquid; wherein the reaction tank is formed with respect to the first eluent injection tank, the sample injection tank, and the detection liquid injection tank The sample liquid carries the outermost edge of the turntable; wherein, according to different types of the test liquid, the metal mark is correspondingly added to the sample injection tank or the detection liquid injection tank; wherein the reaction tank is oriented A reaction tank injection flow path extends from a center of the sample liquid carrying turntable, and the first eluent injection tank, the sample injection tank and the detection liquid injection tank are respectively connected to the reaction tank injection flow path. The detecting device of claim 11, wherein the microchannel structure further comprises a second eluent injection tank for storing the eluent, and the second eluent injection tank is connected to The reaction tank is injected into the flow path. The detecting device of claim 12, wherein the microchannel structure further comprises at least one waste liquid storage tank, and the waste liquid storage tank is respectively connected to the reaction tank, the first eluent An injection tank, the sample injection tank, the detection liquid injection tank, and the second eluent injection tank. The detecting device of claim 13, wherein the number of the waste liquid storage tanks is two, and the two waste liquid storage tanks are respectively adjacent to the left and right sides of the reaction tank.