MX2017011489A - Nanopore detection of small molecules through competition assays. - Google Patents

Nanopore detection of small molecules through competition assays.

Info

Publication number
MX2017011489A
MX2017011489A MX2017011489A MX2017011489A MX2017011489A MX 2017011489 A MX2017011489 A MX 2017011489A MX 2017011489 A MX2017011489 A MX 2017011489A MX 2017011489 A MX2017011489 A MX 2017011489A MX 2017011489 A MX2017011489 A MX 2017011489A
Authority
MX
Mexico
Prior art keywords
detection
target
molecule
small molecules
nanopore
Prior art date
Application number
MX2017011489A
Other languages
Spanish (es)
Inventor
J Morin Trevor
Original Assignee
Two Pore Guys Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Two Pore Guys Inc filed Critical Two Pore Guys Inc
Publication of MX2017011489A publication Critical patent/MX2017011489A/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3278—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Nanotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Disclosed herein are methods and compositions for detection of target small molecules in a mixed sample by performing a competition assay between the target and a surrogate and subsequently detecting the complex types in a nanopore device. Disclosed herein are competition assays for detection of small molecules using a nanopore. Target molecules of a sufficient size (> 20 kDa) when passed through a solid-state nanopore cause a change in the current impedance, translocation time, or other measurable parameter. In the event the target molecule is not sufficiently big, and thus does not cause a noticeable change, an additional molecule/reagent can be used to aid in detection. This detection reagent would bind to the small molecule or to the "capture ligand-molecule complex" (e.g. peptide detection is aided by a monoclonal antibody (mAb) that recognizes a peptide/aptamer complex).
MX2017011489A 2015-03-11 2016-03-11 Nanopore detection of small molecules through competition assays. MX2017011489A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562131693P 2015-03-11 2015-03-11
PCT/US2016/022210 WO2016145415A1 (en) 2015-03-11 2016-03-11 Nanopore detection of small molecules through competition assays

Publications (1)

Publication Number Publication Date
MX2017011489A true MX2017011489A (en) 2018-01-23

Family

ID=56878896

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017011489A MX2017011489A (en) 2015-03-11 2016-03-11 Nanopore detection of small molecules through competition assays.

Country Status (8)

Country Link
US (1) US10837954B2 (en)
EP (1) EP3268732A4 (en)
JP (1) JP6568949B2 (en)
KR (2) KR20190012278A (en)
CN (1) CN107430107B (en)
CA (1) CA2978202A1 (en)
MX (1) MX2017011489A (en)
WO (1) WO2016145415A1 (en)

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JP2018537673A (en) * 2015-11-23 2018-12-20 ツー ポア ガイズ インコーポレイテッド Target modification for tracking and detection
CN107014791B (en) * 2017-03-29 2019-10-29 郑州轻工业学院 A kind of fluorescent optical sensor and its preparation method and application
CN108181358B (en) * 2017-11-15 2020-02-11 华东理工大学 Method for detecting amino acid based on aerolysin nanochannel
DE102018210484A1 (en) 2018-06-27 2020-01-02 Robert Bosch Gmbh Sensor for detecting ions in a fluid and method for detecting ions in a fluid with such a sensor
CN109239140B (en) * 2018-08-16 2021-04-13 广东第二师范学院 Nanopore functionality control method and system
CN109078628B (en) * 2018-08-27 2021-06-11 西北大学 High-efficiency hydrophobic interaction chromatographic medium with benzylamine as ligand, preparation method and application of medium in protein renaturation and purification
EP3847449A4 (en) * 2018-09-07 2022-08-31 Ontera Inc. Sensing compositions, methods, and devices for the detection of molecules using a nanopore device
KR102127111B1 (en) 2019-01-31 2020-06-26 경희대학교 산학협력단 Method for producing a nanopore and nanopore structure using the method
EP3953489A1 (en) * 2019-04-12 2022-02-16 Miltenyi Biotec B.V. & Co. KG Conjugates having an enzymatically releasable detection moiety and a barcode moiety
CN111999490B (en) * 2019-12-19 2021-07-30 瑞芯智造(深圳)科技有限公司 Method for detecting trace protein in sample system
EP4610365A1 (en) * 2024-02-28 2025-09-03 Mugenbio GmbH Single molecule detection system and method

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CN1250741C (en) * 2000-02-03 2006-04-12 研究发展基金会 Signaling aptamers that transducer molecular recognition to a differential signal
US6780584B1 (en) 2000-09-27 2004-08-24 Nanogen, Inc. Electronic systems and component devices for macroscopic and microscopic molecular biological reactions, analyses and diagnostics
US20030022150A1 (en) * 2001-07-24 2003-01-30 Sampson Jeffrey R. Methods for detecting a target molecule
AU2002361642A1 (en) 2001-11-16 2003-06-10 The University Of Tennessee Research Corporation Recombinant antibody fusion proteins and methods for detection of apoptotic cells
CA2505907A1 (en) * 2002-11-14 2004-06-03 Qtl Biosystems, Llc Methods of biosensing using fluorescent polymers and quencher-tether-ligand bioconjugates
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WO2014182634A1 (en) * 2013-05-06 2014-11-13 Two Pore Guys, Inc. A method of biological target detection using a nanopore and a fusion protein binding agent
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Also Published As

Publication number Publication date
CA2978202A1 (en) 2016-09-15
KR101945366B1 (en) 2019-02-07
US20180313814A1 (en) 2018-11-01
JP6568949B2 (en) 2019-08-28
EP3268732A4 (en) 2018-07-25
JP2018508023A (en) 2018-03-22
KR20170118229A (en) 2017-10-24
CN107430107A (en) 2017-12-01
CN107430107B (en) 2019-08-30
HK1246395A1 (en) 2018-09-07
WO2016145415A1 (en) 2016-09-15
EP3268732A1 (en) 2018-01-17
KR20190012278A (en) 2019-02-08
US10837954B2 (en) 2020-11-17

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