JPS578797A - Method and reagent for quantitative determination of free fatty acid - Google Patents
Method and reagent for quantitative determination of free fatty acidInfo
- Publication number
- JPS578797A JPS578797A JP4346381A JP4346381A JPS578797A JP S578797 A JPS578797 A JP S578797A JP 4346381 A JP4346381 A JP 4346381A JP 4346381 A JP4346381 A JP 4346381A JP S578797 A JPS578797 A JP S578797A
- Authority
- JP
- Japan
- Prior art keywords
- reagent
- free fatty
- acyl
- coenzyme
- quantitative determination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003153 chemical reaction reagent Substances 0.000 title abstract 4
- 235000021588 free fatty acids Nutrition 0.000 title abstract 3
- 102000004539 Acyl-CoA Oxidase Human genes 0.000 abstract 2
- 108020001558 Acyl-CoA oxidase Proteins 0.000 abstract 2
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 abstract 2
- 102000005870 Coenzyme A Ligases Human genes 0.000 abstract 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 abstract 2
- 108010011449 Long-chain-fatty-acid-CoA ligase Proteins 0.000 abstract 2
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 abstract 1
- 102000016938 Catalase Human genes 0.000 abstract 1
- 108010053835 Catalase Proteins 0.000 abstract 1
- RGJOEKWQDUBAIZ-IBOSZNHHSA-N CoASH Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCS)O[C@H]1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-IBOSZNHHSA-N 0.000 abstract 1
- 102000003992 Peroxidases Human genes 0.000 abstract 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 abstract 1
- 125000002252 acyl group Chemical group 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- RGJOEKWQDUBAIZ-UHFFFAOYSA-N coenzime A Natural products OC1C(OP(O)(O)=O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-UHFFFAOYSA-N 0.000 abstract 1
- 239000005516 coenzyme A Substances 0.000 abstract 1
- 229940093530 coenzyme a Drugs 0.000 abstract 1
- 238000004737 colorimetric analysis Methods 0.000 abstract 1
- KDTSHFARGAKYJN-UHFFFAOYSA-N dephosphocoenzyme A Natural products OC1C(O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 KDTSHFARGAKYJN-UHFFFAOYSA-N 0.000 abstract 1
- 239000006185 dispersion Substances 0.000 abstract 1
- PGLTVOMIXTUURA-UHFFFAOYSA-N iodoacetamide Chemical compound NC(=O)CI PGLTVOMIXTUURA-UHFFFAOYSA-N 0.000 abstract 1
- 125000005439 maleimidyl group Chemical class C1(C=CC(N1*)=O)=O 0.000 abstract 1
- 230000001590 oxidative effect Effects 0.000 abstract 1
- 108040007629 peroxidase activity proteins Proteins 0.000 abstract 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: To carry out the quantitative determination of free fatty acids, by treating the free fatty acids with an acyl CoA synthetase in the presence of ATP and CoA, oxidizing the produced acyl CoA with an acyl CoA oxidase in the presence of an SH reagent, and determining the produced hydrogen peroxide.
CONSTITUTION: An aqueous solution or dispersion of an optionally diluted specimen is treated with an acyl coenzyme A synthetase, adenosine triphosphate, and coenzyme A at pH7W8 and 37°C for 5W60min. An acyl coenzyme A oxidase, an SH reagent such as a maleimide derivative, iodoacetamide, etc., peroxidase or catalase, and color-developing reagents such as 4-aminoantipyrin and phenol, etc. are added simultaneously or separately to the above treated product, and heated at 37°C for 5W60min. The density of the developed color is determined by the colorimetry using a blank test as a contrast.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4346381A JPS578797A (en) | 1981-03-24 | 1981-03-24 | Method and reagent for quantitative determination of free fatty acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4346381A JPS578797A (en) | 1981-03-24 | 1981-03-24 | Method and reagent for quantitative determination of free fatty acid |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP13701178A Division JPS5564800A (en) | 1978-11-06 | 1978-11-06 | Method and reagent for determination of acyl coenzyme a |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS578797A true JPS578797A (en) | 1982-01-18 |
| JPS5733955B2 JPS5733955B2 (en) | 1982-07-20 |
Family
ID=12664400
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4346381A Granted JPS578797A (en) | 1981-03-24 | 1981-03-24 | Method and reagent for quantitative determination of free fatty acid |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS578797A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01249679A (en) * | 1988-03-29 | 1989-10-04 | Toyo Tanso Kk | Graphite-silicon carbide composite body and production thereof |
| US4978615A (en) * | 1984-04-13 | 1990-12-18 | Kyowa Medex Co., Ltd. | Method for the determination of the compound having mercapto group |
| US5126246A (en) * | 1988-04-15 | 1992-06-30 | Toyo Jozo Company, Ltd. | Reagent for analysis of triglycerides and analysis using the same |
| JP2002051769A (en) * | 2000-08-11 | 2002-02-19 | Azwell Inc | Liquid reagent useful for measuring nonesterified fatty acid |
| US6686172B2 (en) | 2000-06-30 | 2004-02-03 | Azwell, Inc. | Method of measuring total homocysteine |
| US10358257B2 (en) | 2014-07-30 | 2019-07-23 | Ball Corporation | Vented container end closure |
| US10556718B2 (en) | 2013-03-15 | 2020-02-11 | Ball Corporation | End closure with a ring pull actuated secondary vent |
-
1981
- 1981-03-24 JP JP4346381A patent/JPS578797A/en active Granted
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4978615A (en) * | 1984-04-13 | 1990-12-18 | Kyowa Medex Co., Ltd. | Method for the determination of the compound having mercapto group |
| JPH01249679A (en) * | 1988-03-29 | 1989-10-04 | Toyo Tanso Kk | Graphite-silicon carbide composite body and production thereof |
| US5126246A (en) * | 1988-04-15 | 1992-06-30 | Toyo Jozo Company, Ltd. | Reagent for analysis of triglycerides and analysis using the same |
| US6686172B2 (en) | 2000-06-30 | 2004-02-03 | Azwell, Inc. | Method of measuring total homocysteine |
| JP2002051769A (en) * | 2000-08-11 | 2002-02-19 | Azwell Inc | Liquid reagent useful for measuring nonesterified fatty acid |
| US10556718B2 (en) | 2013-03-15 | 2020-02-11 | Ball Corporation | End closure with a ring pull actuated secondary vent |
| US10358257B2 (en) | 2014-07-30 | 2019-07-23 | Ball Corporation | Vented container end closure |
| US10981694B2 (en) | 2014-07-30 | 2021-04-20 | Ball Corporation | Vented container end closure |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5733955B2 (en) | 1982-07-20 |
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