ES470253A1 - A METHOD TO OBTAIN A MODIFIED MICRO-ORGANISM - Google Patents

A METHOD TO OBTAIN A MODIFIED MICRO-ORGANISM

Info

Publication number
ES470253A1
ES470253A1 ES470253A ES470253A ES470253A1 ES 470253 A1 ES470253 A1 ES 470253A1 ES 470253 A ES470253 A ES 470253A ES 470253 A ES470253 A ES 470253A ES 470253 A1 ES470253 A1 ES 470253A1
Authority
ES
Spain
Prior art keywords
nucleotide sequence
mrna
gene
cdna
desired gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES470253A
Other languages
Spanish (es)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California Berkeley
University of California San Diego UCSD
Original Assignee
University of California Berkeley
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US05897710 external-priority patent/US4363877B1/en
Application filed by University of California Berkeley, University of California San Diego UCSD filed Critical University of California Berkeley
Publication of ES470253A1 publication Critical patent/ES470253A1/en
Expired legal-status Critical Current

Links

Landscapes

  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

A method for obtaining a modified microorganism for containing a nucleotide sequence with the structure of a gene of a higher organism, transcribed from that gene, which method comprises: a) isolating cells of a higher organism containing mrna that encodes the desired gene; b) extract the mrna from the cells in the presence of a rnasa inhibitor composition, thereby preventing mrna degradation by rnasa. C) separating the mrna essentially free of protein, dna and other rnas, d) synthesizing a cdna or double helix, where the chain contains a nucleotide sequence complementary to that of the mrna, thereby producing cdna with a nucleotide sequence encoding the desired gene, e) provide a dna transfer vector with reactive ends capable of being linked together or of being joined with double-stranded cdna, f) attaching the dna transfer vector to the double-stranded cdna containing a sequence of nucleotides encoding the desired gene, and g) combining a microorganism with the vector of dna transfer bound to the cdna with a nucleotide sequence encoding the desired gene, thereby producing a microorganism with a nucleotide sequence encoding the gene wanted. (Machine-translation by Google Translate, not legally binding)
ES470253A 1977-05-27 1978-05-26 A METHOD TO OBTAIN A MODIFIED MICRO-ORGANISM Expired ES470253A1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US80134377A 1977-05-27 1977-05-27
US80502377A 1977-06-09 1977-06-09
US89770978A 1978-04-19 1978-04-19
US05897710 US4363877B1 (en) 1977-09-23 1978-04-19 Recombinant dna transfer vectors

Publications (1)

Publication Number Publication Date
ES470253A1 true ES470253A1 (en) 1979-09-16

Family

ID=27505812

Family Applications (2)

Application Number Title Priority Date Filing Date
ES470253A Expired ES470253A1 (en) 1977-05-27 1978-05-26 A METHOD TO OBTAIN A MODIFIED MICRO-ORGANISM
ES470254A Expired ES470254A1 (en) 1977-05-27 1978-05-26 A method of preparation of a transfer vector (Machine-translation by Google Translate, not legally binding)

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES470254A Expired ES470254A1 (en) 1977-05-27 1978-05-26 A method of preparation of a transfer vector (Machine-translation by Google Translate, not legally binding)

Country Status (1)

Country Link
ES (2) ES470253A1 (en)

Also Published As

Publication number Publication date
ES470254A1 (en) 1979-10-01

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