CN101950501B - Model for carrying out virtual navigation and assisting tumor tissue biopsy process being unable to be displayed ultrasonically - Google Patents

Abstract

The invention belongs to the field of medical and health care, and relates to a model for the biopsy process of tumor tissue that cannot be displayed by ultrasound aided by virtual navigation. The model is composed of tumor glue and parenchyma glue, and the parenchyma glue is made of carrageenan; the tumor glue is added by carrageenan Made of iodized oil, surfactant and milk. The model is one or more tumor glue blocks cut into volumes of 0.5-2 cubic centimeters wrapped in parenchymal glue. The model can accurately simulate the tumor tissue and its surrounding normal tissues that cannot be displayed by ultrasound in the virtual navigation technology, and effectively guide the virtual navigation technology in real time to assist in positioning the tumor tissue that cannot be displayed by ultrasound. The tissue biopsy results obtained through this model It is accurate and easy to operate, and achieves the purpose of learning and training applied to virtual navigation technology.

Description

A model for virtual navigation-assisted biopsy of tumor tissue that cannot be visualized by ultrasound

technical field

The invention belongs to the medical and health field, relates to the field of ultrasound-guided interventional therapy in ultrasound medicine, and in particular relates to a model used for virtual navigation-assisted tumor tissue biopsy process that cannot be displayed by ultrasound.

Background technique

Ultrasound-guided biopsy of liver tumor tissue is a commonly used technique in clinical practice. In this technique, ultrasound mainly plays a role in locating the tumor. The precondition for using ultrasound guidance is that ultrasound can display tumors. However, due to factors such as liver tissue background disorder and iso-echoic lesions, it is often encountered in practice that ultrasound cannot display tumor lesions. The imaging principle of CT is different from that of ultrasound. When ultrasound cannot show the tumor, CT can often show it. However, the current CT is static imaging, which is not suitable for guiding tissue biopsy in real time.

Virtual navigation technology can use magnetic positioning methods to fuse image information of different types or at different times, such as the fusion of 3D ultrasound volume images and CT 3D volume images of the same patient or the fusion of 3D ultrasound volume images of the same patient at different times. After fusion, the three-dimensional data displayed by the two images can completely overlap in space, and the two overlapping images can be displayed on the same screen at the same time, and clinically useful information can be found by comparing the two images.

Virtual navigation technology successfully combines ultrasound real-time imaging and CT high-resolution imaging, and extracts the advantages of both through image fusion technology. CT images can be used to assist ultrasound in real-time positioning to assist tumors that cannot be displayed by ultrasound in biopsy , is a technology with high clinical application value.

The principle of virtual navigation technology is completely different from other imaging methods in the past, which contains more concepts of physics, such as spatial magnetic positioning, image fusion, etc. Such concepts are abstract and difficult to be understood by imaging doctors, which affects the mastery of this useful technology.

In vitro models can play an important role in scaling up a new technology. Some foreign scholars have used commercialized colloids to make virtual navigation-guided ultrasound phantoms that cannot display tumor biopsies, but such ultrasound phantoms are expensive and cannot be reused. Another foreign scholar used isolated bovine liver to make a model, but this model needs to use CT to evaluate the puncture accuracy, the operation is relatively complicated, and the isolated tissue is relatively elastic, and it is easy to deform during the operation, which will affect the accuracy of puncture. Therefore, it is not suitable for learning and training of virtual navigation technology. At present, there is no relevant research report in China.

Contents of the invention

The purpose of the present invention is to address the deficiencies of the prior art, to provide a model for virtual navigation-assisted tumor tissue biopsy process that cannot be displayed by ultrasound, and to promote learning and training of virtual navigation technology.

Another object of the present invention is to provide a preparation method of the model.

The above-mentioned purpose of the present invention is achieved through the following technical solutions:

The invention provides a model for the biopsy process of tumor tissue that cannot be displayed by virtual navigation-assisted ultrasound, which is characterized in that the model is composed of one or more tumor glue blocks wrapped in parenchymal glue; the parenchymal glue is made of carrageenan , tumor glue is made by mixing carrageenan, lipiodol, surfactant and milk.

Above-mentioned two kinds of glues are made by following raw material and method respectively:

The parenchyma gum is prepared by mixing a carrageenan-physiological saline solution obtained by mixing dry carrageenan and hot physiological saline according to a mass ratio of 1:45-55, and then cooling it.

Tumor glue is made of carrageenan-physiological saline solution mixed with lipiodol, surfactant and milk according to the mass ratio (150-400): 1: (3-5): (15-40) (to become carrageenan-physiological brine-iodol-surfactant-milk solution), and made by cooling.

In this model, the tumor glue is cut into small pieces with a volume of 0.5-2 cubic centimeters, and the cut tumor glue pieces are wrapped with parenchymal glue.

Marking material is also attached to the real glue, which is a similar toothpick-shaped article made of bamboo, wood or plastic, and the preferred marking material is a toothpick.

The present invention simultaneously provides the preparation method of this model, comprises the following steps:

(1) Prepare carrageenan-normal saline solution and carrageenan-normal saline-iodol-surfactant-milk solution respectively.

(2) After the carrageenan-normal saline-iodol-iodol-surfactant-milk solution is cooled and solidified, it is cut into tumor gel pieces with a volume of 0.5-2 cubic centimeters; the tumor glue pieces are placed in the carrageenan-normal saline solution After cooling, make a model. After the carrageenan-physiological saline solution is cooled and shaped, a marking item is inserted therein as an alignment mark.

The colloid used in the present invention has the following advantages in the application of virtual navigation-assisted tissue biopsy process:

(1) The present invention has selected carrageenan as the main raw material of the model. Carrageenan is a polysaccharide extracted from seaweed. Its food-grade dry colloid is cheap and is a commonly used material in the food and pharmaceutical industries. The carrageenan produced in Hainan, my country belongs to the □-family. This group of carrageenan has good thermal reversibility. The melting point of solid carrageenan and the freezing point of liquid carrageenan are between 40-60°C, that is, simple heating or cooling can Carrageenan for reshaping. This kind of colloid has the advantages of easy availability, low price, low requirements for production conditions, good sound permeability, and the solid glue is not easily deformed. The carrageenan-physiological saline solution with a suitable ratio has suitable hardness, elasticity and brittleness after being solidified, and it is not easy to deform after being made into a model, and can be made into a real glue.

(2) The present invention uses lipiodol as an additive to the tumor glue. Lipiodol can significantly increase the density of the tissue, and the addition of colloid can increase the CT value. Combining surfactant with lipiodol, so that lipiodol is evenly distributed in liquid carrageenan, after such glue becomes solid, its ultrasonic echo will increase unevenly, but the CT value will increase significantly. The tumor gel was embedded in the parenchyma, because the ultrasonic echoes of the two gels were the same, so the ultrasound could not show the tumor gel; but the CT value of the tumor gel was significantly higher than that of the parenchyma, so the CT scan could show the tumor gel in the parenchyma. of tumor glue.

(3) Add an appropriate amount of milk to the carrageenan solution with lipiodol added to make the colloid turn milky white, which can distinguish the tumor glue from the parenchymal glue with the naked eye; the ultrasonic echo of the colloid will not change significantly after adding milk.

Carrageenan-normal saline solution is added with iodized oil, surfactant and milk to make tumor glue. After embedding the tumor glue block in ordinary parenchymal glue, the glue block cannot be displayed by ultrasonic scanning, but it can be seen by CT scan. clearly displayed. Ordinary parenchymal glue is colorless and translucent when observed with the naked eye, while tumor glue is white and opaque, which can be clearly distinguished with the naked eye.

Therefore, the model of the present invention can accurately simulate tumor tissue that cannot be displayed by ultrasound in the virtual navigation technology, effectively and objectively guide tissue biopsy in real time: tumor glue, which cannot be displayed by ultrasound, can be displayed by CT scan, and is used to simulate tumors Lesions; the parenchymal glue wrapped around the tumor glue can be displayed by ultrasound and CT and can be distinguished from the tumor glue with the naked eye, which can simulate the normal tissue around the tumor; the tumor glue in the model (simulating tumor lesions) can be biopsied, and the detected glue The strips can be observed with the naked eye and are clearly different from the parenchymal glue to verify the effect of virtual navigation-assisted biopsy. At the same time, the invention further explores the material placed in the real rubber block model as a virtual navigation alignment mark, which can be a toothpick-shaped object made of bamboo, wood or plastic, and it is found that it can be clearly displayed on the ultrasonic image. Invention chooses to use ordinary toothpicks. After the parenchymal glue embeds the tumor glue block and solidifies, insert a toothpick into the parenchymal glue. The toothpick can be clearly displayed on the ultrasound image and can be used for alignment marks.

Compared with the prior art, the present invention has the following beneficial effects:

(1) The model provided by the present invention can accurately simulate tumor tissues that cannot be displayed by ultrasound but can be displayed by CT, effectively and objectively simulate virtual navigation technology to assist in positioning tumor tissues that cannot be displayed by biopsy ultrasound, and realize the improvement of virtual navigation technology. Learn and train effectively.

(2) The biopsy result obtained by the model of the present invention is accurate and the biopsy operation process is simple and convenient.

(3) The manufacturing cost of the model provided by the present invention is low, and the raw materials used are all low-cost raw materials and have a wide range of sources.

(4) The preparation method of the model of the present invention is simple and easy, does not need large-scale or advanced equipment, and has strong implementability

Description of drawings

Figure 1 shows the strips detected by the biopsy needle, the top one is the parenchymal strip (shown by the left arrow), the left half of the middle strip is the tumor strip, and the right half is the parenchymal strip (shown by the right arrow) , the bottom is the tumor glue strip (shown by the upward arrow), and different glue strips can be distinguished by naked eyes.

Figure 2 is the ultrasound image and CT image fusion, the left picture is the ultrasound picture of the model, no tumor glue is seen in the model, the right picture is the CT picture of the model, the tumor glue is seen in the model (indicated by the upward arrow).

Detailed ways

The technical solution of the present invention is further illustrated below through specific examples.

Example

Dissolve the dry product of carrageenan produced in Hainan in hot normal saline at a ratio of 1:50, and after fully dissolved, it becomes a carrageenan-normal saline solution for use.

Lipiodol, detergent, milk and carrageenan-normal saline solution were mixed at a ratio of 1:5:20:200, cooled and solidified to form a tumor glue. The solidified tumor glue was cut into 1 cubic centimeter glue block, put into carrageenan-normal saline solution, and after the solution was solidified, bamboo toothpicks were scattered and vertically inserted into the parenchyma to make a model.

The side of the rubber block marked with a toothpick is the head end for CT scanning. Import the CT image in Dicom format into the Mylab 90 ultrasonic instrument. The bamboo toothpick is high-density in the CT image and hyperechoic in the ultrasonic image. Use this as a positioning mark. After fusing the ultrasonic and CT images, use navigation assistance Carry out tumor gel biopsy (as shown in Figure 2).

After the biopsy is completed, verify the results of the virtual navigation-assisted biopsy by observing the biopsy strips. Different results can be distinguished with the naked eye: the fully transparent parenchymal strips represent normal tissues; the semi-transparent and semi-milky white semi-parenchymal strips represent semi-lesions Semi-tumor strips; full milky-white tumor strips representing complete lesions (as shown in Figure 1).

From the results of the naked eye observation after the biopsy, it can be clearly known that the virtual navigation technology assists in locating the tumor biopsy tumor tissue that cannot be displayed by ultrasound, so as to achieve the purpose of learning and training the virtual navigation technology.

Claims (6)

1. model that is used for the tumor tissues biopsy procedure that the virtual navigation assisting ultrasonic can't show is characterized in that this model is made up of one of essence glue parcel or above tumour glue; Described essence glue is processed by carragheen; Tumour glue is mixed and made into by carragheen, lipiodol, surfactant and milk; Described essence glue is that carragheen dry product and hot physiological saline are stirred, process after the cooling according to carragheen-normal saline solution that the mixed of mass ratio 1: 45-55 obtains; Described tumour glue be by carragheen-normal saline solution and lipiodol, surfactant, milk according to mass ratio 150-400: 1: 3-5: the mixed of 15-40, cooling are processed.

2. model as claimed in claim 1, the fritter that to it is characterized in that described tumour glue be volume 0.5-2 cubic centimetre is with essence glue parcel one or above tumour blob of viscose.

3. model as claimed in claim 1 is characterized in that attach mark material on the described essence glue.

4. model as claimed in claim 3 is characterized in that described marker material is the article of bamboo, wooden or plastic similar toothpick shape.

5. Preparation of model method that is used for virtual navigation aid in tissue biopsy procedure is characterized in that may further comprise the steps:

(1) prepares carragheen-normal saline solution respectively, carragheen-physiological saline-lipiodol-surfactant-milk soln;

(2) with cutting into the tumour glue blob of viscose that volume is the 0.5-2 cubic centimetre after carragheen-physiological saline-lipiodol-surfactant-milk soln cooled and solidified; Tumour glue blob of viscose is placed mould, pour behind carragheen-normal saline solution cooling into and process model;

Described carragheen-physiological saline is that carragheen dry product and the hot physiological saline mixed according to mass ratio 1: 45-55 is obtained; Described carragheen-physiological saline-lipiodol-surfactant-milk soln be with carragheen-normal saline solution and lipiodol, surfactant and milk according to mass ratio 150-400: 1: 3-5: the mixed of 15-40 obtains.

6. preparation method as claimed in claim 5, it is characterized in that the described carragheen of step (2)-normal saline solution cooling forming after, insert the article of bamboo, wooden or plastic similar toothpick shape more therein.

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