Papers by mohammed albujja
Preimplantation genetic testing for embryos predisposed to hereditary cancer: Possibilities and challenges
Cancer Pathogenesis and Therapy
Evaluation of skin surface sample as an alternative source to traditional samples to collect DNA reference sample
Journal of Forensic Research, Oct 30, 2013
Short tandem repeats on X and Y chromosomes in relation to prostate cancer risks in Saudi male population
Prediction of the susceptible population to any hereditary disease is one of the main tracks of h... more Prediction of the susceptible population to any hereditary disease is one of the main tracks of human genetics. Although there were studies related between prostate cancer (PrCa) and family hereditary or Y-chromosome alleles frequencies and other studies related between X-chromosome alleles frequency and PrCa, most of them were inconclusive because of the limited number of biomarkers and/or samples as well as the investigation of sex chromosomes markers separately. Forensic short tandem repeats (STRs) are sometimes best to seek information from lineage markers to aid with specific inheritance scenarios. Therefore, many studies were conducted to establish an association between phenotype and genotype in that disease by Y STR lineages. Lineage markers including Y and X-chromosomes markers can aid kinship analysis. Therefore, in the current study it was hypothesized that the analyses of both X and Y-chromosomes together besides increasing the number of investigated markers to 35 markers (23 in Y-chromosome and 12 in X-chromosome) will give comprehensive linkage information between sex chromosomes and PrCa. Most of the previous studies compare PrCa patients only and healthy controls. Also, they examine the genetic biomarkers on Y-chromosome only. While the current study included PCa and benign prostate hyperplasia (BPH) patients as well as examined the biomarkers on X and Y-chromosomes. Multiple comparisons were done, three of them between (malignant, benign, and malignant plus benign patients), respectively, and healthy controls group. One more comparison was established between benign and malignant patients. This research was carried to fill a gap in research by the identification of a genetic biomarker able to predict men’s susceptibility to malignant or benign disease. To the best of our knowledge, this study is the first to analyze 23 DYS loci in PrCa in a Saudi male population and the first to analyze 12 DXS loci in PrCa patients in a Saudi male population, and worldwide. To perform this study, 248 subjects were recruited, comprising 92 patients and 156 healthy controls. All DNA samples were amplified using PowerPlex® Y23 System and Investigator® Argus X-12 QS. The fragments analysis for alleles of 23 DYS and 12 DXS loci were performed using Genetic Analyzer. In this study, the results emphasize the influence of genetic elements on PrCa and some alleles of DYS391, DYS437, DYS390, DYS458, DYS481, DYS389II, DYS393 and DYS570 loci in Y-chromosome, as well as some alleles of DXS10135, DXS10146, DXS10148, DXS10079, DXS10103, DXS10101, DXS7132, DXS10134, DXS7423, and HPRTB loci in X-chromosome in Saudi male population. These genetic biomarkers have the potential to be used as a screening method for prediction of susceptibility to PrCa in the Saudi men population, especially those who has high value (> 4 ng/mL) in the PSA test. From the comparisons between Y-chromosome haplotypes comprising different alleles of clusters (1, 2, 3a, and 3b) for 23 DYS loci, it is likely that men who belong to the Y-chromosome lineages with certain haplotypes have a higher risk to develop PrCa or BPH, compared with males having other haplotypes. While, males belonging to lineages with [12, 22, 10, 11] haplotype of cluster 3a are more protected against PrCa either benign or malignant. Furthermore, the comparisons between X-chromosome haplotypes comprising different alleles of clusters (X1, X2, X3, X4), and (lowest GD loci) for 12 DXS loci showed that it is likely that men who belong to the X-chromosome lineages with particular haplotypes have a significantly higher risk to develop PrCa or BPH compared with males having other haplotypes. The obvious observation in the results of this study that, microvariant alleles (18.2, and 19.2) and (17.2, and 20.2) of DYS458 locus are overrepresented in the PrCa and PBH group in comparison to none in the control group with high OR results; hence, suggesting that men carrying these microvariant alleles of DYS458 locus are at increased risk to develop PrCa or BPH, respectively. Furthermore, microvariant alleles of some X-chromosome loci are overrepresented in BPH group in comparison to none in the healthy control and non in PrCa groups, hence suggesting that men carrying these microvariant alleles are at increased risk to develop BPH. Only one microvariant allele (31.2) of DXS10101 locus is presented more in PrCa group in comparison to BPH group. Therefore, loci that have significant differences in allele frequencies between patients and healthy controls group could be in linkage disequilibrium with tumor-related genes. Although further studies need to be done to confirm this hypothesis
Arab Journal of Forensic Sciences and Forensic Medicine, 2015
The impact of chimerism on DNA-based human identification from skin surface cells of post-allogenic hematopoietic stem cell transplantation (HCST) patients
The use of biological traces recovered from touched or handled items increased with the advance o... more The use of biological traces recovered from touched or handled items increased with the advance of the forensic analysis system. Thus, DNA profiles obtained from touch DNA became a useful tool in forensic investigation. However, a chimeric person with more than one chromosomal population can be challenging for a forensic analyst. We investigated the genetic profile in blood, buccal swab, and skin swabs from twenty-four recipients aged 21-63 years who underwent a matched sibling allogeneic hematopoietic stem cell transplantation with no sign of skin graft versus host disease. Autosomal short tandem repeats genotyping was performed to evaluate chimerism status at 15 loci along with gender marker Amelogenin. According to our results, donor chimerism was detected in all recipient's blood samples, while in buccal swabs, five recipients showed no presence of donor-derived cells in their genotype. Epithelial cells swabbed from hand fingertips were not devoid of donor-derived cells sinc...
Population genetic data for 12 X-STR loci in the Central Saudi region using investigator Argus X-12 amplification kit
Annals of Human Biology, 2021
Abstract Background X-chromosome short tandem repeat (X-STR) markers are important in forensic id... more Abstract Background X-chromosome short tandem repeat (X-STR) markers are important in forensic identity investigations and kinship analysis. Subject and methods In the current study, the distribution of 12 X-STR loci located in four linkage groups was evaluated using Investigator® Argus X-12 Amplification Kit in 200 unrelated healthy individuals (105 males and 95 females) from the central region of Saudi Arabia in order to develop an allelic frequency database for the markers included in the kit. Results DXS10146 locus was the most informative with 21 alleles, while DXS8378 locus was the least with five alleles. Forensic parameters showed that all X-STRs loci, either as individual markers or as linkage groups, provide genetic information with high discrimination that is appropriate for forensic purposes with polymorphism information content (PIC), power of exclusion (PE), and paternity index (PI) varying from 0.61211 to 0.917979, 0.38722 to 0.842949, and 0.038416 to 0.16367, respectively. The pairwise genetic distance fixation index (Fst) results showed that the Saudi population is genetically closer to the Egyptian and Emirati populations and distant to the Turkish population. Conclusion The current study revealed that Investigator® Argus 12 X-STR kit would support the forensic application, kinship testing involving female offspring, and human identification in the Saudi population.
Arab Journal of Forensic Sciences and Forensic Medicine, 2021
The last three decades have seen rapid advances in the field of short tandem repeats (STRs) genot... more The last three decades have seen rapid advances in the field of short tandem repeats (STRs) genotyping technology. Autosomal STRs have emerged as a powerful tool in forensic identification and paternity investigations. The indigenous population of Saudi Arabia is irregularly distributed and has historically been organized into geographically distinct groups or tribes of patrilineal descent. So far, there has been no detailed investigation of the southern region Saudi population to assist in the interpretation of DNA-based forensic evidence and in the construction of DNA database. The objective of this study is to investigate the genetic structure in 154 unrelated healthy Saudi subjects within three generations from the southern Saudi regions using a GlobalFiler™ PCR Amplification kit. Intra- and Inter-population genetic diversity as well as the forensic genetics parameters were analyzed. Our results showed that SE33 and TPOX loci were the most and the least polymorphic loci, respect...
BMC Complementary Medicine and Therapies
Inflammation is the main key role in developing chronic diseases including cancer, cardiovascular... more Inflammation is the main key role in developing chronic diseases including cancer, cardiovascular diseases, diabetes, arthritis, and neurodegenerative diseases which possess a huge challenge for treatment. With massively compelling evidence of the role played by nutritional modulation in preventing inflammation-related diseases, there is a growing interest into the search for natural functional foods with therapeutic and preventive actions. Honey, a nutritional healthy product, is produced mainly by two types of bees: honeybee and stingless bee. Since both types of honey possess distinctive phenolic and flavonoid compounds, there is recently an intensive interest in their biological and clinical actions against inflammation-mediated chronic diseases. This review shed the light specifically on the bioavailability and bioaccessibility of honey polyphenols and highlight their roles in targeting inflammatory pathways in gastrointestinal tract disorders, edema, cancer, metabolic and card...
Asian Pacific Journal of Cancer Prevention
Background: The X-chromosome has been suggested to play a role in prostate cancer (PrCa) since ep... more Background: The X-chromosome has been suggested to play a role in prostate cancer (PrCa) since epidemiological studies have provided evidence for an X-linked mode of inheritance for PrCa based on the higher relative risk among men who report an affected brother(s) as compared to those reporting an affected father. The aim of this study was to examine the potential association between the forensic STR markers located at four regions Xp22.31, Xq11.2-12, Xq26.2, and Xq28 and the risk of BPH and PrCa to confirm the impact of ChrX in the PrCa incidence. This may be helpful in the incorporation of STRs genetic variation in the early detection of men population at risk of developing PrCa. Methods: DNA samples from 92 patients and 156 healthy controls collected from two medical centers in Riyadh, Saudi Arabia were analyzed for four regions located at X-chromosome using the Investigator ® Argus X-12 QS Kit. Results: The results demonstrated that microvariant alleles of (DXS7132, DXS10146, HPRTB, DXS10134, and DXS10135) are overrepresented in the BPH group (p < 0.00001). Allele 28 of DXS10135 and allele 15 of DXS7423 could have a protective effect, OR 0.229 (95%CI, 0.066-0.79); and OR 0.439 (95%CI, 0.208-0.925). On the other hand, patients carrying allele 23 of DXS10079 and allele 26 of DXS10148 presented an increased risk to PrCa OR 4.714 (95%CI, 3.604-6.166). Conclusion: The results are in concordance with the involvement of the X chromosome in PrCa and BPH development. STR allele studies may add further information from the definition of a genetic profile of PrCa resistance or susceptibility. As TBL1, AR, LDOC1, and RPL10 genes are located at regions Xp22.31, Xq11.2-12, Xq26.2, and Xq28, respectively, these genes could play an essential role in PrCa or BPH.
Acute inflammation and oxidative stress induced by lipopolysaccharide and the ameliorative effect of stingless bee honey
Combinatorial Chemistry & High Throughput Screening
Background: Systemic acute inflammation is the hallmark of sepsis and associated with multiple or... more Background: Systemic acute inflammation is the hallmark of sepsis and associated with multiple organ dysfunction. Objective: This study investigated the potential of stingless bee honey (SBH) to suppress lipopolysaccharide (LPS)-induced systemic acute inflammation in rats and to reveal the probable mechanism of action. Methods: Rats received 4.6 and 9.2 g/kg SBH for 7 days followed by a single injection of LPS and samples were collected after 6 h. Results: LPS induced liver, kidney, heart and lung injury manifested by increased serum transaminases, alkaline phosphatase, creatine kinase, creatinine and urea, along with multiple histological alterations, particularly, leukocyte infiltrations. Pro-inflammatory cytokines were elevated in serum, and NF-κB p65, p38 MAPK and HMGB-1 were significantly increased in different tissues of LPS-challenged rats. SBH prevented tissue injury, ameliorated pro-inflammatory cytokines, and suppressed NF-κB p65, p38 MAPK and HMGB-1 in rats received LPS. ...
A review of studies examining the association between genetic biomarkers (STRs and SNPs) and Risk of Prostate Cancer: the need for valid predictive biomarkers
Prostate International
Evaluation of Skin Surface as an Alternative Source of Reference DNA Samples: A Pilot Study
Journal of forensic sciences, Jan 23, 2017
An acceptable area for collecting DNA reference sample is a part of the forensic DNA analysis dev... more An acceptable area for collecting DNA reference sample is a part of the forensic DNA analysis development. The aim of this study was to evaluate skin surface cells (SSC) as an alternate source of reference DNA sample. From each volunteer (n = 10), six samples from skin surface areas (forearm and fingertips) and two traditional samples (blood and buccal cells) were collected. Genomic DNA was extracted and quantified then genotyped using standard techniques. The highest DNA concentration of SSC samples was collected using the tape/forearm method of collection (2.1 ng/μL). Cotton swabs moistened with ethanol yielded higher quantities of DNA than swabs moistened with salicylic acid, and it gave the highest percentage of full STR profiles (97%). This study supports the use of SSC as a noninvasive sampling technique and as a extremely useful source of DNA reference samples among certain cultures where the use of buccal swabs can be considered socially unacceptable.
Induction of Chronic Subclinical Systemic Inflammation in Sprague–Dawley Rats Stimulated by Intermittent Bolus Injection of Lipopolysaccharide
Archivum Immunologiae et Therapiae Experimentalis
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Papers by mohammed albujja