After consultation with the authors, the symbols and molecular descriptions of the trx and ash1 alleles in Table 1 of FBrf0233053 have been updated as follows: 1. trx alleles A. Symbol updates FBid new FlyBase symbol old FlyBase symbol symbol in Table 1 FBal0338142 trxAM1 trxR1578mut trxR1578* FBal0338140 trxAM2 trxS2582a trxS2582Amber FBal0338141 trxAM3 trxS2582b trxS2582Amber B. Molecular characterization updates trxAM2 (trxS2582a in the table) and trxAM3 (trxS2582b in the table) are both listed at S2582Amber mutations but are actually Q2582Amber (codon 2582 is a Gln, not a Ser). Both result from a C->T substitution at 3R:14 ,268,577 that changes CAG(Gln) into TAG(Amber). Summary table FB symbol nuc. change predicted aa change trxAM2 C14268577T Q2582term trxAM3 C14268577T Q2582term 2. ash1 alleles The amino acid locations reported for the ash1 mutations are 9 amino acids off relative to the current ash1 gene annotation which was significantly updated in 2011. At that time, based on cDNA and RNA-seq exon junction data, the splice donor of the first coding exon was updated to use a GC splice donor 11 bases downstream of the originally annotated GT splice donor. The change in splice donor changed the reading frame which necessitated the use of an alternative upstream ATG start. The first coding exon currently encodes 28 amino acids rather than 19 as originally annotated which changed the amino acid numbering for all of the downstream amino acids. A. Symbol updates: FBid new FlyBase symbol old FlyBase symbol symbol in Table 1 FBal0338137 ash1AM1 ash1N303mut ash1N303* FBal0338139 ash1AM2 ash1W770mut ash1W770* FBal0338135 ash1AM3 ash1W790term ash1W790* FBal0338136 ash1AM4 ash1Q893term ash1Q893* FBal0338138 ash1AM5 ash1H1873W ash1H1873W B. Molecular characterization updates Summary table FB symbol nuc. change predicted aa change ash1AM3 G19595839A W779term ash1AM4 C9595471T Q902term ash1AM5 C19592468T H1882Y ash1B1 C19593107T Q1669term Allele details For allele ash1AM3 (W790* in Table 1) The change is at position 3R:19 ,595,839. G->A. This changes a TGG(W) to a TAG(amber) at amino acid #779 (current annotation). For allele ash1AM4 (Q893* in Table 1) The change is at position 3R:19 ,595,471. C->T. This changes a CAA(Q) to a TAA(ochre) at amino acid #902 (current annotation). This could now be called Q902* or Q902term. For allele ash1AM5 (H1873W in Table 1) The change is at position 3R:19 ,592,468. C->T. This changes a CAT(H) to a TAT(Y=tyrosine) at amino acid #1882 (current annotation). This was reported as H->W in Table 1 but is actually H->Y. For allele ash1B1 (Q1660* in Table 1) The change is at position 3R:19 ,593,107. C->T. This changes a CAG(Q) to a TAG(amber) at amino acid #1669 (current annotation).