Isolation and characterization of Dp(1;Y)BSC276
Kim Cook, Russell Garton, Adam Brown, Megan Ward, Jennifer Deal, Megan Deal, Thom Kaufman & Kevin Cook
Bloomington Drosophila Stock Center
Indiana University
Dp(1;Y)BSC276 was isolated following irradiation of C(1;Y)N12, In(1)BSC16, P{w+mC=3'.RS5+3.3'}BSC16 w1118, BS, a chromosome with the following form:
1Lt to 1B5|14A9 to 1B5|14A9 to h28-h29|YS to YL|BS
Dp(1;Y)BSC276 has the following form
1Lt to 1A5-1B5|19C6-19D1 to h28-h29|YS to YL|BS
The entire inverted region was deleted as well as most X euchromatin. The X portion of Dp(1;Y)BSC276 consists of only X tip and X base segments. The same result could have been obtained by irradiating C(1;Y)N12 without an X inversion.
The distal X segment extends from the X telomere to an unmapped point between the yellow (y) gene and the distal In(1)BSC16 breakpoint at 1B5. Consequently, it encompasses 1Lt to  X:255278--387562  (Release 5), which corresponds to 1Lt;1A5--1B5.
The proximal X segment extends from  X:20264299--20300545  (which corresponds to 19C6--19D1) to X heterochromatin bands h28--h29. The 19C6--19D1 breakpoint was localized relative to transposon insertion sites by PCR amplification as described in Cook et al. (FBrf0212582). Specifically, it mapped between the following transposons and primer pairs.
Insertion: P{Mae-UAS.6.11}GG01176
Forward primer: CATCCAGCTTAGGCTTGATCCTGC ( X:20264299..20264322 )
Reverse primer: GCATTTCAATTTACGTGACGGTGTG ( X:20265056..20265080 )
Insertion: PBac{WH}CG11710f03997
Forward primer: GTGATTACACAGTCCAGACATTTGG ( X:20300545..20300569 )
Reverse primer: GTTGCGCCATACTCTTCTTCGC ( X:20301193..20301214 )
The deletion of the entire inverted region of In(1)BSC16 is evidenced by the absence the miniwhite marker in P{w+mC=3'.RS5+3.3'}BSC16, which is associated with the distal In(1)BSC16 breakpoint, and the failure to amplify several PCR fragments from region 14AC within the inversion. In particular, the following primers located 2.5 kb from the In(1)BSC16 breakpoint failed to amplify a fragment:
Forward primer: GGGAATTTCCTTGCAGCGCTTAGTGG ( X:15983253..15983278 )
Reverse primer: GGCATTTTGTCGCGGTGTTCGTCGC ( X:15984029..15984053 )
Dp(1;Y)BSC276 was retained for the Bloomington Stock Center collection, because it carries a particularly large duplicated segment from the base of the X chromosome.