Isolation and characterization of Df(3R)BSC505
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC505 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG1792f06161 and P{XP}d09534. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}CG1792f06161/P{XP}d09534 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC505 from the segment of PBac{WH}CG1792f06161 to the left of its FRT site and the segment of P{XP}d09534 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of PBac{WH}CG1792f06161 to be Release 3 genomic coordinate 27432828 on chromosome arm 3R and the insertion site of P{XP}d09534 to be Release 3 genomic coordinate 27561025 on chromosome arm 3R. The Gene Disruption project determined the insertion site of PBac{WH}CG1792f06161 to be Release 3 genomic coordinate 27432863 on arm 3R. The PBac{WH}CG1792f06161 insertion site corresponds to 100C1 on the Release 3 genome map and 100D1 on the Release 5 genome map.  The P{XP}d09534 insertion site corresponds to 100C3 on the Release 3 genome map and 100D2 on the Release 5 genome map. Consequently, the cytological breakpoints of Df(3R)BSC505 are predicted to be 100D1;100D2. Df(3R)BSC505 failed to complement ttk1e11.