Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant fusion protein in bacterial system.
Chromatin cross-linked RNA was incubated with biotin-labeled lncRNA:roX2 probes. E(z)-lncRNA:roX2 complexes were pulled down with streptavadin.
Source was cell extract of S2 cell line; bait and prey produced from endogenous gene.