FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
mir-let7, Imp
FlyBase ID
FBig0000108374
Interaction Network
Interactions Browser links
mir-let7 network
Imp network
Reported Interactions
FBrf0218483-3.miR-mRNA.IHC
Reference
Toledano et al., 2012
Description
physical association
Assay
Collection
Source/Stage
hub cell / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-let7 

let-7

bait
Imp 

Imp

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Overexpression of let-7 reduces Imp protein levels.

(Toledano et al., 2012)

Source was adult males testes (hub cell); bait produced from transgenic construct; prey produced from endogenous gene.

(Toledano et al., 2012)
FBrf0218483-4.miR-mRNA.GFP
Reference
Toledano et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
mir-let7 

let-7

bait
mir-let7 
Imp 

Imp

prey
Imp 
Imp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Imp 
3-prime UTR
sufficient binding region
Imp 
let-7 target site
necessary binding region
UACUGUUGAUACUACCUCC

seed: UACCUC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

GFP reporter assay

(Toledano et al., 2012)

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

(Toledano et al., 2012)
External Crossreferences and Linkouts ( 0 )
References (1)