FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Tctp, tefu
FlyBase ID
FBig0000101141
Interaction Network
Interactions Browser links
Tctp network
tefu network
Reported Interactions
FBrf0223709-1.FPD
Reference
Hong and Choi, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tctp 

dTCTP

bait
tefu 

dATM

prey
tefu 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tefu 
dTCTP-interacting peptide heptamer
sufficient binding region
KCCYATK
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein in bacteriophage system from random peptide library.

(Hong and Choi, 2013)
FBrf0223709-10.coIP.WB
Reference
Hong and Choi, 2013
Description
physical association
Assay
Collection
Source/Stage
organism / prepupal stage P1
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tctp 

dTCTP

bait
tefu 

dATM

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was white prepupal nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Hong and Choi, 2013)

The interaction of Tctp with tefu is resistant to benzonase digestion of DNA, which eliminates non-specific interactions via chromatin.

(Hong and Choi, 2013)
FBrf0223709-2.PD.MW
Reference
Hong and Choi, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tefu 

dATM

bait

GST tag

tefu 
tefu 
Tctp 

dTCTP

prey

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tefu 
dTCTP-interacting region
sufficient binding region
aa 1006-1115
tefu 
dTCTP-interacting region
necessary binding region
aa 1081-1106

FPYFLCYSTKSEFRK mutated to LE

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Hong and Choi, 2013)
FBrf0223709-3.PD.MW
Reference
Hong and Choi, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tctp 

dTCTP

bait

GST tag

Tctp 
Tctp 
Tctp 
tefu 

dATM

prey

MBP tag

tefu 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tefu 
dTCTP-interacting region
sufficient binding region
aa 1006-1215
Tctp 
C-terminal region
sufficient binding region
aa 121-172

\'C\'

Tctp 
middle region
sufficient binding region
aa 61-120

\'M\'

Tctp 
N-terminal region
mutation decreasing interaction
aa 12

E12V

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Hong and Choi, 2013)

Binding of tefu by the middle or C-terminal regions of Tctp is enhanced by Tctp\'s N-terminal region.

(Hong and Choi, 2013)
FBrf0223709-4.coIP.WB
Reference
Hong and Choi, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Tctp 

dTCTP

bait

Myc tag

tefu 

dATM

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hong and Choi, 2013)
FBrf0223709-7.coIP.WB
Reference
Hong and Choi, 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
tefu 

dATM

bait

FLAG tag

Tctp 

dTCTP

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Hong and Choi, 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)