FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Syx5, αSnap
FlyBase ID
FBig0000008025
Interaction Network
Interactions Browser links
Syx5 network
αSnap network
Reported Interactions
FBrf0151889-1.Y2H
Reference
Xu et al., 2002
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
αSnap 

dSNAP

bait

fused to GAL4 DNA-binding domain

Syx5 

dSyx5

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from ovarian cDNA expression library).

(Xu et al., 2002)

Five independent clones of Syx5 recovered in the screen for αSnap interacting proteins.

(Xu et al., 2002)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Xu et al., 2002)
FBrf0151889-2.PD.WB
Reference
Xu et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Syx5 

dSyx5

bait

GST tag

αSnap 

dSNAP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Xu et al., 2002)
FBrf0151889-3.PD.WB
Reference
Xu et al., 2002
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Syx5 

dSyx5

bait

GST tag

αSnap 

dα-SNAP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type adult head extract.

(Xu et al., 2002)
FBrf0218395-2487.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Syx5 
unspecified role
Syx5 
unspecified role
Syx5 
unspecified role
αSnap 
unspecified role
αSnap 
unspecified role
αSnap 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 227.61303

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)