FlyBase Gene Report: Dmel\Orc1

General Information

Symbol

Dmel\Orc1

Species

D. melanogaster

Name

Origin recognition complex subunit 1

Annotation Symbol

CG10667

Feature Type

protein_coding_gene

FlyBase ID

FBgn0286788

Gene Model Status

Current

Stock Availability

10 publicly available

Gene Summary

Origin recognition complex subunit 1 (Orc1) encodes the largest subunit of the origin recognition complex (ORC), which is essential for the initiation of DNA replication in eukaryotic cells. The ATPase activity of the product of Orc1 is critical for the formation and function of the pre-replicative complex. The regulated degradation of the product of Orc1 by ubiquitin-mediated proteolysis after initiation of DNA replication helps to prevent re-replication. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Also Known As

DmORC1, ORC, sat

Key Links

Genomic Location

Cytogenetic map

43D1-43D2

Sequence location

2R:7,522,437..7,525,716 [-]

Recombination map

2-56

RefSeq locus

NT_033778 REGION:7522437..7525716

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (10 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR003959

(InterPro Project Members, 2004-)

enables ATP hydrolysis activity

inferred from electronic annotation with InterPro:IPR003959

(InterPro Project Members, 2004-)

enables chromatin binding

inferred from electronic annotation with InterPro:IPR001025

(InterPro Project Members, 2004-)

enables DNA replication origin binding

inferred from biological aspect of ancestor with PANTHER:PTN000080056

(GO Reference Genome Project, 2011-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in DNA amplification

inferred from mutant phenotype

(Park and Asano, 2008)

involved_in DNA replication initiation

inferred from direct assay

(Chesnokov et al., 1999)

NOT involved_in DNA endoreduplication

inferred from mutant phenotype

(Park and Asano, 2008)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in DNA replication initiation

inferred from biological aspect of ancestor with PANTHER:PTN000080056

(GO Reference Genome Project, 2011-)

involved_in mitotic DNA replication checkpoint signaling

inferred from biological aspect of ancestor with PANTHER:PTN000080056

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

part_of nuclear origin of replication recognition complex

inferred from direct assay

(Chesnokov et al., 1999)

located_in nucleus

inferred from direct assay

(Kopytova et al., 2016)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of nuclear origin of replication recognition complex

inferred from biological aspect of ancestor with PANTHER:PTN000080057

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

ORIGIN RECOGNITION COMPLEX

UNCLASSIFIED ATPASES

Protein Family (UniProt)

Belongs to the ORC1 family. (O16810)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

ATP hydrolysis activity
RHEA 13065:

Summaries

Gene Snapshot

Gene Group (FlyBase)

ORIGIN RECOGNITION COMPLEX -: The Origin Recognition Complex (ORC) is a multi-subunit complex that binds replication origin during replication initiation. Many ORC subunits belong to the AAA+ superfamily of ATPases. (Adapted from FBrf0227879).
UNCLASSIFIED ATPASES -: This group comprises ATPases that do not classify under other groups in FlyBase.

Protein Function (UniProtKB)

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.

(UniProt, O16810)

Summary (Interactive Fly)

A subunit of the Origin recognition complex, regulating the initiation of DNA replication - Orc1 is degraded by APC at the cell's exit from mitosis and reaccumulates at the end of G1

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Orc1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry O16810)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

Comments on Gene Model

Gene model reviewed during 5.48

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Orc1-RA

FBtr0088959

NM_057955

3152

924

Additional Transcript Data and Comments

Reported size (kB)

2.7 (northern blot)

(Chesnokov et al., 1999)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Orc1-PA

FBpp0088033

103.3

924

9.84

O16810

NP_477303

AAF59236

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

924 (aa)

(Pak et al., 1997)

Comments

External Data

Subunit Structure (UniProtKB)

ORC is composed of six subunits.

(UniProt, O16810)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Orc1 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.30

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13

embryonic brain

(Asano and Wharton, 1999)

anterior midgut primordium

(Asano and Wharton, 1999)

posterior midgut primordium

(Asano and Wharton, 1999)

larval ventral nerve cord

(Asano and Wharton, 1999)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage & larval stage

(Chesnokov et al., 1999)

Additional Descriptive Data

Orc1 transcripts are maternally deposited and are abundant in early embryos. Levels taper off in later embryonic and larval stages.

(Chesnokov et al., 1999)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 4

organism | uniform

• nucleus

(Asano and Wharton, 1999)

embryonic stage 5 -- 17

mitotic domain

• nucleus

(Asano and Wharton, 1999)

embryonic stage 6

ventral furrow

• nucleus

(Asano and Wharton, 1999)

embryonic stage 13

embryonic brain

• nucleus

(Asano and Wharton, 1999)

larval ventral nerve cord

• nucleus

(Asano and Wharton, 1999)

third instar larval stage

eye disc posterior to the morphogenetic furrow

• nucleus

(Asano and Wharton, 1999)

adult stage | female oogenesis stage S6 -- S10B

nurse cell

• nucleus

(Asano and Wharton, 1999)

follicle cell

• nucleus

(Asano and Wharton, 1999)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 15

organism

Comment: strongest expression 0-6 hrs AEL; sharply reduced by 9-12 hr AEL

(Asano and Wharton, 1999)

Additional Descriptive Data

Orc1 protein is present in the nuclei of mitotically active cells

(Asano and Wharton, 1999)

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of nuclear origin of replication recognition complex

inferred from direct assay

(Chesnokov et al., 1999)

located_in nucleus

inferred from direct assay

(Kopytova et al., 2016)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Orc1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Orc1

View exonic expression by tissue for Dmel\Orc1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35686/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0286788

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0022772

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0286788

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10667

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 29 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Orc1

Transgenic constructs containing regulatory region of Orc1

Aberrations (Deficiencies and Duplications) ( 11 )

Inferred from experimentation ( 11 )

Gene disrupted in

Df(2R)ED1673

(Kahsai and Cook, 2018)

Df(2R)ED1715

(Kahsai and Cook, 2018)

Df(2R)Exel6052

(Kahsai and Cook, 2018, Ryder, 2004.4.26)

Df(2R)k08815

(Kahsai and Cook, 2018)

Df(2R)P32a

(Schupbach and Wieschaus, 1991)

Df(2R)pk78s

(Schupbach and Wieschaus, 1991)

Df(2R)ST1

(Schupbach and Wieschaus, 1991)

Gene not disrupted in

Df(2R)BSC263

(Kahsai and Cook, 2018)

Df(2R)BSC264

(Kahsai and Cook, 2018)

Df(2R)ED1725

(Kahsai and Cook, 2018)

Df(2R)Exel6053

(Kahsai and Cook, 2018)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

Orc1^hs.PA

lethal, with Scer\GAL4^insc-Mz1407

Orc1^GD4477

lethal (with Df(2R)Exel6052)

Orc1^KO

lethal (with Df(2R)k08815)

Orc1^KO

lethal - all die during pupal stage

Orc1^KO

lethal - all die during pupal stage (with Df(2R)ST1)

Orc1^KO

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Orc1^GD4477

viable, with Scer\GAL4^Mef2.PR

Orc1^GD4477

viable, with Scer\GAL4^pnr-MD237

Orc1^GD4477

viable, with Scer\GAL4^tin.CΔ4

Orc1^GD4477

Sterility

Allele

female sterile

male sterile

Orc1^hs.PA

Other Phenotypes

Allele

abnormal developmental rate

Orc1^KO

abnormal developmental rate (with Df(2R)ST1)

Orc1^KO

abnormal mitotic cell cycle

Orc1^KO

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

Orc1^GD4477

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Orc1^GD4477

decreased cell number

Orc1^KO

decreased cell number | somatic clone

Orc1^KO

decreased occurrence of cell division

Orc1^KO

some die during embryonic stage

Orc1^hs.PA

some die during larval stage

Orc1^hs.PA

some die during pupal stage

Orc1^hs.PA

visible

Orc1^hs.PA

visible, with Scer\GAL4^pnr-MD237

Orc1^GD4477

Phenotype manifest in

Allele

dorsal appendage

Orc1^hs.PA

egg

Orc1^hs.PA

egg chorion

Orc1^hs.PA

embryonic/larval salivary gland

Orc1^KO

eye

Orc1^hs.PA

eye-antennal disc | posterior

Orc1^hs.PA

eye disc morphogenetic furrow

Orc1^hs.PA

follicle cell & nucleus

Orc1^hs.PA

follicle cell | somatic clone

Orc1^KO

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

Orc1^GD4477

imaginal disc

Orc1^KO

interommatidial bristle

Orc1^hs.PA

larval brain

Orc1^KO

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

Orc1^GD4477

mesonotum, with Scer\GAL4^pnr-MD237

Orc1^GD4477

ommatidium

Orc1^hs.PA

oocyte | oogenesis stage S10B

Orc1^hs.PA

trichogen cell, with Scer\GAL4^pnr-MD237

Orc1^GD4477

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (4)

Hsap\ORC1

12 of 14

Yes

Hsap\CDC6

4 of 14

Hsap\ORC4

1 of 14

Hsap\RFC2

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (5)

Rnor\Orc1

12 of 14

Yes

4 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (5)

Mmus\Orc1

11 of 14

Yes

4 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (3)

Xtro\orc1

9 of 13

Yes

Xtro\cdc6

1 of 13

Xtro\rfc4

1 of 13

Danio rerio (Zebrafish) (4)

Drer\orc1

12 of 14

Yes

Drer\cdc6

4 of 14

Drer\orc4

1 of 14

Drer\rfc2

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\orc-1

10 of 14

Yes

Cele\cdc-6

4 of 14

Anopheles gambiae (African malaria mosquito) (5)

Agam\AgaP_AGAP004957

12 of 12

Yes

3 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (8)

Atha\ORC1A

10 of 13

Yes

Atha\ORC1B

10 of 13

Yes

4 of 13

4 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\ORC1

10 of 13

Yes

Scer\SIR3

6 of 13

Yes

Scer\CDC6

3 of 13

Scer\ORC4

1 of 13

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\orc1

9 of 12

Yes

Spom\cdc18

3 of 12

Spom\orc4

1 of 12

Spom\rfc3

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Orc1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (3)

Cdc6

6 of 13

Orc4

1 of 13

RfC4

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

ORC1; origin recognition complex subunit 1

model of Meier-Gorlin syndrome 1

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ORC1; origin recognition complex subunit 1

12 of 14

601902

MEIER-GORLIN SYNDROME 1; MGORS1

Meier-Gorlin syndrome 1

CDC6; cell division cycle 6

4 of 14

602627

MEIER-GORLIN SYNDROME 5; MGORS5

Meier-Gorlin syndrome 5

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Orc1 - Cdc6

pull down, molecular weight estimation by staining, electron microscopy

(Schmidt and Bleichert, 2020, Bleichert et al., 2018)

Orc1 - E2f1

pull down, western blot

(Bosco et al., 2001)

Orc1 - Myb

anti bait coimmunoprecipitation, western blot

(Beall et al., 2002)

Orc1 - Orc2

cross-linking study, Identification by mass spectrometry, electron microscopy, anti bait coimmunoprecipitation, western blot, x-ray crystallography, pull down, molecular weight estimation by staining

(Schmidt and Bleichert, 2020, Bleichert et al., 2018, Bleichert et al., 2015, Bleichert et al., 2013, Beall et al., 2002)

Orc1 - Orc3

x-ray crystallography, pull down, molecular weight estimation by staining, electron microscopy

(Schmidt and Bleichert, 2020, Bleichert et al., 2018, Bleichert et al., 2015, Bleichert et al., 2013)

Orc1 - Orc4

electron microscopy, x-ray crystallography, pull down, autoradiography, molecular weight estimation by staining, cross-linking study, Identification by mass spectrometry

(Schmidt and Bleichert, 2020, Bleichert et al., 2018, Bleichert et al., 2015, Bleichert et al., 2013)

Orc1 - Orc5

electron microscopy, pull down, molecular weight estimation by staining, cross-linking study, Identification by mass spectrometry, x-ray crystallography

(Schmidt and Bleichert, 2020, Bleichert et al., 2018, Bleichert et al., 2015, Bleichert et al., 2013)

Orc1 - Orc6

fluorescence polarization spectroscopy, predetermined participant, pull down, molecular weight estimation by staining, electron microscopy, x-ray crystallography

(Schmidt and Bleichert, 2020, Bleichert et al., 2018, Bleichert et al., 2015, Bleichert et al., 2013)

Orc1 - Rbf

pull down, western blot

(Bosco et al., 2001)

Orc1 - Su(var)205

pull down, autoradiography

(Pak et al., 1997)

Orc1 - cav

pull down, autoradiography

(Badugu et al., 2003)

Orc1 - e(y)2

anti bait coimmunoprecipitation, peptide massfingerprinting

(Kopytova et al., 2016)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

ORC is composed of six subunits.

(UniProt, O16810 )

Linkouts

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-56

Cytogenetic map

43D1-43D2

Sequence location

2R:7,522,437..7,525,716 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

43D1-43D2

Limits computationally determined from genome sequence between P{lacW}cos^k16101 and P{EP}CG1600^EP398

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

The sat complementation group maps to the genomic interval 2R:7493197..7533553 , release 6 genome (left limit from complementation mapping against Df(2R)Exel6052, right limit from complementation mapping against Df(2R)Exel6053).

(Kahsai and Cook, 2018)

43E1-43E2

(determined by in situ hybridisation)

(Chesnokov et al., 1999)

43D1-43D7

(determined by in situ hybridisation)

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-56

(FlyBase, 2016)

2-56.7

(Comeron et al., 2012)

2-57

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

5108

Orc1^SC46 cn¹ bw¹/CyO, P{sevRas1.V12}FK1

79775

y¹ sc^* v¹ sev²¹; P{TKO.GS01816}attP40

64592

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05627}attP40

77867

w^*; TI{TI}Orc1^KO/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7

FlyORF

F001524

M{UAS-Orc1.ORF}ZH-86Fb

F001139

M{UAS-Orc1.ORF.3xHA}ZH-86Fb

NIG-Fly

10667R-3

P{NIG.10667R}3

2RG-0940

y² VhaAC39-1^cho-1 v¹; P{U6-Orc1.NIG.sgRNA}attP40/CyO

VDRC

v46521

w¹¹¹⁸; P{GD4477}v46521

v46522

w¹¹¹⁸; P{GD4477}v46522

Genomic Clones (12)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (47)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD11626

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Asano and Wharton, 1999, Chesnokov et al., 1999)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

New stable cell line derived from S2-unspecified : The following constructs were used to create stable S2 cell lines: Full-length Drosophila Orc1 (generated from cDNA), Drosophila Orc1[IDR] (residues 187-549), Drosophila Orc1[IDR-P-dead] (residues 187-549 with every '[S/T]P' mutated to 'AP') and metazoan Orc1[IDR] orthologs [human Orc1 residues 177-484, Fasciola hepatica (Platyhelminthes) Orc1 residues 1-267, Hypsibius dujardini (Tardigrada) Orc1 residues 1-278 and Amphimedon queenslandica (Porifera) Orc1 residues 177-593].
(Adiji et al., 2024)

Other Comments

Orc1 is essential for mitotic replication and proliferation in brains and imaginal discs, as well as for gene amplification in ovarian follicle cells. Orc1 is not required for endoreplication or cell viability.

(Park and Asano, 2008)

The ATP dependent DNA binding activity of the Orc1/Orc2 protein complex is reduced or inhibited by phosphorylation of Orc1.

(Remus et al., 2005)

Orc1 is degraded by the APC (anaphase-promoting complex).

(Araki et al., 2003)

Orc1 is required for ATP hydrolysis and ATP-dependent DNA binding.

(Chesnokov et al., 2001)

Transcription of Orc1 is coupled to cell cycle progression via direct regulation by E2f. The level of Orc1 protein appears to limit origin utilisation in at least two different cell types.

(Asano and Wharton, 1999)

Mutations at the sat locus cause defects in midoogenesis.

(Schupbach and Wieschaus, 1991)

Relationship to Other Genes

Source for database merge of

Source for merge of: Orc1 sat

(Kahsai and Cook, 2018)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Orc1 CG10667

(Gabrielian, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

CG10667

(National Institute of Genetics Fly Stocks, 2022-, Schertel et al., 2013, Müller et al., 2010, Perkins et al., 2009)

DmORC1

(Crevel et al., 2005, Bosco et al., 2001, Chesnokov et al., 1999, Cavalli and Paro, 1998, Chesnokov, 1997.8.6, Pak et al., 1997)

DmOrc1

(Clarey et al., 2006)

DmOrc1p

(Remus et al., 2005)

ORC

(Schwaiger, 2006, Bielinsky et al., 2001, Wakimoto, 1998, Huang and Kellum, 1997)

ORC1

(Richards et al., 2022, Meyer-Nava et al., 2020, Maksimenko et al., 2018, Hua and Orr-Weaver, 2017, Bitard-Feildel et al., 2015, Joyce et al., 2012, Park and Asano, 2008, Araki et al., 2005, Badugu et al., 2005, Araki et al., 2002, Chesnokov et al., 2002, Li et al., 2002, Royzman et al., 2002, Eissenberg and Elgin, 2000, Asano et al., 1999, Wharton, 1999.3.23, Wallrath, 1998)

dORC1

(Burke et al., 2001)

fs(2)SC46

orc1

(Parker et al., 2019, Longworth et al., 2012, Park and Asano, 2012, Park and Asano, 2011, Park and Asano, 2008, Foglietti et al., 2006)

rDmORC

(Remus et al., 2004)

sat

(Kahsai and Cook, 2018)

Name Synonyms

Origin recognition complex subunit 1

(Toshima et al., 2014)

origin recognition complex 1

(Park and Asano, 2008)

satin

Secondary FlyBase IDs

FBgn0022772
FBgn0000998
FBgn0004792

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 46 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35686

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

O16810

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

O16810

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

O16810

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35686/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0286788

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0286788

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG10667

MARRVEL_MODEL - MARRVEL (model organism gene)

35686

PDB - An information portal to biological macromolecular structures

Linkouts

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0022772

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0286788

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10667

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC008879

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (119)

The following external sites may use different assemblies or annotations than FlyBase.

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