FlyBase Gene Report: Dmel\Maf1

General Information

Symbol

Dmel\Maf1

Species

D. melanogaster

Name

Maf1

Annotation Symbol

CG40196

Feature Type

protein_coding_gene

FlyBase ID

FBgn0267861

Gene Model Status

Current

Stock Availability

2 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Also Known As

dMaf1

Key Links

Genomic Location

Cytogenetic map

41A1-41A1

Sequence location

2R:1,512,810..1,527,095 [-]

Recombination map

2-55

RefSeq locus

NT_033778 REGION:1512810..1527095

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

NCBI

UCSC

Ensembl

Function

Gene Ontology (GO) Annotations (3 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables RNA polymerase III core binding

inferred from biological aspect of ancestor with PANTHER:PTN000500453

(GO Reference Genome Project, 2011-)

Biological Process (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in negative regulation of transcription by RNA polymerase III

inferred from mutant phenotype

(Filer et al., 2017, Rideout et al., 2012)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in negative regulation of transcription by RNA polymerase III

inferred from biological aspect of ancestor with PANTHER:PTN000500453

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR015257

(InterPro Project Members, 2004-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000500453

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Maf1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q7PL26)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.55

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Low-frequency RNA-Seq exon junction(s) not annotated.

Pseudogene similar to CG40196; partial.

May correspond to a redundant fragment in U, rather than a pseudogene.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Maf1-RA

FBtr0113829

NM_001015167

1155

226

Maf1-RB

FBtr0113831

NM_001015168

1358

226

Maf1-RC

FBtr0113830

NM_001110544

4125

226

Maf1-RD

FBtr0301121

NM_001170411

1102

226

Maf1-RE

FBtr0344972

NM_001300720

1078

226

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Maf1-PA

FBpp0112552

25.7

226

4.70

FBpp0112554

25.7

226

4.70

FBpp0112553

25.7

226

4.70

FBpp0290343

25.7

226

4.70

FBpp0311225

25.7

226

4.70

Q7PL26

NP_001287649

EYR77331

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

226 aa isoforms: Maf1-PA, Maf1-PB, Maf1-PC, Maf1-PD, Maf1-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q7PL26

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Maf1 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.19

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Maf1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Maf1

View exonic expression by tissue for Dmel\Maf1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

3354925/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0267861

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0058196

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0267861

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG40196

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Maf1

Transgenic constructs containing regulatory region of Maf1

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Other Phenotypes

Allele

abnormal developmental rate, with Scer\GAL4^Cg.PA

Maf1^RNAi.UAS

abnormal developmental rate, with Scer\GAL4^da.PU

Maf1^RNAi.UAS

abnormal developmental rate, with Scer\GAL4^ppl.PP

Maf1^RNAi.UAS

increased body size | pupal stage, with Scer\GAL4^da.PU

Maf1^RNAi.UAS

long lived, with Scer\GAL4^TIGS-2

Maf1^UAS.Tag:HA

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (1)

Hsap\MAF1

14 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (1)

Rnor\Maf1

14 of 14

Yes

Mus musculus (laboratory mouse) (1)

Mmus\Maf1

13 of 14

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\maf1

6 of 13

Yes

Danio rerio (Zebrafish) (2)

Drer\maf1a

14 of 14

Yes

Drer\maf1b

12 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\mafr-1

14 of 14

Yes

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP008411

12 of 12

Yes

Arabidopsis thaliana (thale-cress) (1)

Atha\AT5G13240

13 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\MAF1

10 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\maf1

12 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Maf1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MAF1; MAF1 negative regulator of RNA polymerase III

14 of 14

610210

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Maf1 - Brf

anti bait coimmunoprecipitation, western blot

(Marshall et al., 2012)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Maf1

suppressible

InR

(Rideout et al., 2012)

Maf1

suppressible

RpS3

(Rideout et al., 2012)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

MIST (protein-protein) - An integrated Molecular Interaction Database

3354925

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Regulation of PTEN gene transcription

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-55

Cytogenetic map

41A1-41A1

Sequence location

2R:1,512,810..1,527,095 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

41A1-41A1

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

h59-h61

(Drosophila Heterochromatin Genome Project, 2004)

Experimentally Determined Recombination Data

Location

2-55

(FlyBase, 2016)

2-55.3

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (2)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

64603

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05638}attP40

VDRC

v109142

P{KK115881}VIE-260B

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (8)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Annotation CR41604 eliminated in release 6.01 of the genome annotation.

(FlyBase Genome Annotators, 2014)

Maf1 is a repressor of RNA polymerase III-dependent tRNA transcription.

(Rideout et al., 2012)

New annotation (CR41604) in release 5.2 of the genome annotation.

(Drosophila Heterochromatin Genome Project, 2007)

New annotation (CG40196) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Relationship to Other Genes

Source for database merge of

Source for merge of: Maf1 CR41604

(FlyBase Genome Annotators, 2014)

Additional comments

CR41604 corresponds to a redundant unmapped fragment (in release 5) that maps to the Maf1 locus.

(FlyBase Genome Annotators, 2014)

Nomenclature History

Source for database identify of

Source for identity of: Maf1 CG40196

(Rideout et al., 2012)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Name Synonyms

Maf1

Secondary FlyBase IDs

FBan0040196
FBgn0058196
FBan0041604
FBgn0085814

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 33 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

3354925

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AABU01002543

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q7PL26

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q7PL26

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q7PL26

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

3354925/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0267861

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0267861

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG40196

MARRVEL_MODEL - MARRVEL (model organism gene)

3354925

Linkouts

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0058196

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0267861

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG40196

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC014322

MIST (protein-protein) - An integrated Molecular Interaction Database

3354925

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Regulation of PTEN gene transcription

References (28)

The following external sites may use different assemblies or annotations than FlyBase.

-1.19

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