FlyBase Gene Report: Dmel\nub

General Information

Symbol

Dmel\nub

Species

D. melanogaster

Name

nubbin

Annotation Symbol

CG34395

Feature Type

protein_coding_gene

FlyBase ID

FBgn0085424

Gene Model Status

Current

Stock Availability

92 publicly available

Gene Summary

nubbin (nub) encodes a POU/homeodomain transcription factor that is expressed in the nascent wing primordium and is required for wing formation. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Also Known As

Pdm1, pdm-1, Pdm, POU domain protein 1, dPOU-19

Key Links

Genomic Location

Cytogenetic map

33F1-33F2

Sequence location

2L:12,587,625..12,628,135 [+]

Recombination map

2-47

RefSeq locus

NT_033779 REGION:12587625..12628135

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (15 terms)

Molecular Function (6 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables DNA-binding transcription activator activity, RNA polymerase II-specific

inferred from direct assay

(Junell et al., 2007)

enables transcription cis-regulatory region binding

inferred from direct assay

(Dantoft et al., 2013)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables DNA binding

inferred from electronic annotation with InterPro:IPR001356, InterPro:IPR010982, InterPro:IPR013847

(InterPro Project Members, 2004-)

enables DNA-binding transcription factor activity, RNA polymerase II-specific

inferred from biological aspect of ancestor with PANTHER:PTN000180816

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR017970

(InterPro Project Members, 2004-)

enables DNA-binding transcription factor activity

inferred from electronic annotation with InterPro:IPR000327

(InterPro Project Members, 2004-)

enables RNA polymerase II cis-regulatory region sequence-specific DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN000180816

(GO Reference Genome Project, 2011-)

Biological Process (7 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in appendage morphogenesis

inferred from mutant phenotype

(Turchyn et al., 2011)

involved_in dendrite morphogenesis

inferred from mutant phenotype

(Iyer et al., 2013)

involved_in negative regulation of antibacterial peptide biosynthetic process

inferred from mutant phenotype

(Dantoft et al., 2013)

involved_in regulation of neural precursor cell proliferation

inferred from genetic interaction with FLYBASE:pdm2; FB:FBgn0004394

(Bahrampour et al., 2017)

involved_in regulation of neurogenesis

inferred from mutant phenotype

(Bahrampour et al., 2017)

involved_in regulation of transcription by RNA polymerase II

inferred from mutant phenotype

(Dantoft et al., 2013)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in regulation of DNA-templated transcription

inferred from electronic annotation with InterPro:IPR000327, InterPro:IPR017970

(InterPro Project Members, 2004-)

involved_in regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000180816

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Brewster et al., 2001)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of RNA polymerase II transcription regulator complex

inferred from biological aspect of ancestor with PANTHER:PTN000180816

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

POU HOMEOBOX TRANSCRIPTION FACTORS

Protein Family (UniProt)

Belongs to the POU transcription factor family. Class-2 subfamily. (P31368)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

nubbin (nub) encodes a POU/homeodomain transcription factor that is expressed in the nascent wing primordium and is required for wing formation. [Date last reviewed: 2019-03-14]

Gene Group (FlyBase)

POU HOMEOBOX TRANSCRIPTION FACTORS -: POU homeobox transcription factors are sequence-specific DNA binding proteins that regulate transcription. They are characterized by a POU-specific domain N-terminal to a homeodomain. POU proteins can bind as homodimers or heterodimers to DNA. (Adapted from FBrf0232555 and PMID:18797923).

Protein Function (UniProtKB)

DNA-binding regulatory protein implicated in early development. Involved in neuronal cell fate decision. Repressed directly or indirectly by the BX-C homeotic proteins.

(UniProt, P31368)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

nub: nubbin

Wings very small, opaque, curved spoonlike up or down; inflated at eclosion. Wing margins interrupted. Only one vein (L2 or L3) present. Halteres somewhat reduced. Viability excellent. RK1.

nub2

Wings small and spoonlike but less extreme than nub. Patches of dried blood on wings. Veins L1 to L4 almost indiscernible; L5 and alula frequently absent. Viability and fertility excellent. RK1.

nub62d

Wings very small and spoonlike. RK1.

Summary (Interactive Fly)

transcription factor - homeodomain and pou domain - involved in cell fate determination of a subset of temporally determined neural subtypes - morphology of sensory neurons is diversified through a series of suppressive transcriptional interactions involving Nubbin and Pdm2, Cut and transcription factors Scalloped and Vestigial - Nubbin and Teashirt mark barriers to clonal growth along the proximal-distal axis of the wing

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\nub for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P31368)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.51

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

nub-RB

FBtr0112642

NM_001103683

4497

961

nub-RD

FBtr0112643

NM_057311

3267

601

nub-RE

FBtr0342628

NM_001298947

2829

601

Additional Transcript Data and Comments

Reported size (kB)

3.3, 2.9, 2.8 (northern blot)

(Billin et al., 1991)

3.1 (northern blot)

(Dick et al., 1991)

4.9, 3.1, 2.6 (northern blot)

(Lloyd and Sakonju, 1991)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

nub-PB

FBpp0111554

103.9

961

6.71

FBpp0111555

65.2

601

9.35

FBpp0309559

65.2

601

9.35

P31368

NP_001285876

AHN54390

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

601 aa isoforms: nub-PD, nub-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

601 (aa); 65 (kD)

(Billin et al., 1991, Dick et al., 1991, Lloyd and Sakonju, 1991)

Comments

nub protein was shown to be a weak octamer-dependent activator of transcription when expressed in yeast and is thought to require additional coactivators to function efficiently.

(Prakash et al., 1992)

The pdm2 and nub proteins are most closely related to the human OCT1 and OCT2 proteins and most resemble class II POU proteins.

(Dick et al., 1991)

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\nub using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.51

Transcript Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

ectoderm | striped

(Dick et al., 1991)

embryonic/larval hypopharynx

(Dick et al., 1991)

embryonic/larval labral segment sense organ

(Dick et al., 1991)

adult optic lobe

(Dick et al., 1991)

central nervous system | restricted

(Dick et al., 1991)

labral segment

(Dick et al., 1991)

embryonic stage 5

parasegment 11

(Lloyd and Sakonju, 1991)

embryonic stage 9

organism | segment polarity expression pattern

(Lloyd and Sakonju, 1991)

embryonic stage 11 -- 17

Comment: reference states >=6 hr AEL

(Lloyd and Sakonju, 1991)

embryonic stage 12

abdominal dorsal bipolar neuron dbp

(Umesono et al., 2002)

multidendritic neuron precursor cell

(Umesono et al., 2002)

embryonic stage 16

abdominal dorsal bipolar neuron dbp

(Umesono et al., 2002)

second instar larval stage

(Lloyd and Sakonju, 1991)

pupal stage

(Lloyd and Sakonju, 1991)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic brain

(Dick et al., 1991)

presumptive embryonic/larval peripheral nervous system | restricted

(Dick et al., 1991)

embryonic/larval pharynx

(Dick et al., 1991)

ventral pharyngeal sense organ

(Dick et al., 1991)

neurogenic region

(Dick et al., 1991)

embryonic stage 4 -- 6

anlage in statu nascendi

(Fisher et al., 2012)

clypeo-labral anlage in statu nascendi

(Fisher et al., 2012)

dorsal ectoderm anlage

(Fisher et al., 2012)

organism | gap expression pattern

(Fisher et al., 2012)

ventral ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 5

parasegment 7

(Lloyd and Sakonju, 1991)

organism | 30-50% egg length

Comment: resolved into 2 bands

(Berman et al., 2004, Dick et al., 1991)

embryonic stage 6

organism | odd pair rule expression pattern

(Lloyd and Sakonju, 1991)

embryonic stage 7 -- 8

clypeo-labral anlage

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

ventral epidermis primordium

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 9 -- 12

midline primordium | subset

(Kearney et al., 2004)

embryonic stage 11

MP3 precursor

(Wheeler et al., 2006)

larval MP1 neuron

(Wheeler et al., 2006)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

epipharynx primordium

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

hypopharynx proper primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 12

ventral pharyngeal sense organ

(Lloyd and Sakonju, 1991)

embryonic stage 12 -- 13

embryonic abdominal lateral sensory organ precursor cluster of abdominal segment 2

(Lloyd and Sakonju, 1991)

embryonic abdominal lateral sensory organ precursor cluster of abdominal segment 3

(Lloyd and Sakonju, 1991)

embryonic abdominal lateral sensory organ precursor cluster of abdominal segment 4

(Lloyd and Sakonju, 1991)

embryonic abdominal lateral sensory organ precursor cluster of abdominal segment 5

(Lloyd and Sakonju, 1991)

embryonic abdominal lateral sensory organ precursor cluster of abdominal segment 6

(Lloyd and Sakonju, 1991)

embryonic abdominal lateral sensory organ precursor cluster of abdominal segment 7

(Lloyd and Sakonju, 1991)

embryonic thoracic lateral sensory organ precursor cluster of prothoracic segment

(Lloyd and Sakonju, 1991)

embryonic thoracic dorsal sensory organ precursor cluster of mesothoracic segment

(Lloyd and Sakonju, 1991)

embryonic thoracic dorsal sensory organ precursor cluster of metathoracic segment

(Lloyd and Sakonju, 1991)

abdominal neuroblast of abdominal segment 2

(Lloyd and Sakonju, 1991)

abdominal neuroblast of abdominal segment 3

(Lloyd and Sakonju, 1991)

abdominal neuroblast of abdominal segment 4

(Lloyd and Sakonju, 1991)

abdominal neuroblast of abdominal segment 5

(Lloyd and Sakonju, 1991)

abdominal neuroblast of abdominal segment 6

(Lloyd and Sakonju, 1991)

abdominal neuroblast of abdominal segment 7

(Lloyd and Sakonju, 1991)

scolopidial ligament cell of embryonic abdominal lateral sensory organ precursor cluster

(Lloyd and Sakonju, 1991)

chordotonal organ of embryonic thoracic lateral sensory organ precursor cluster

(Lloyd and Sakonju, 1991)

presumptive embryonic/larval central nervous system | restricted

(Lloyd and Sakonju, 1991)

embryonic/larval labral segment sense organ | precursor

(Lloyd and Sakonju, 1991)

abdominal neuroblast of abdominal segment 1

(Lloyd and Sakonju, 1991)

presumptive embryonic/larval peripheral nervous system | restricted

(Lloyd and Sakonju, 1991)

scolopidial ligament cell of embryonic thoracic dorsal sensory organ precursor cluster

(Lloyd and Sakonju, 1991)

abdominal dorsal bipolar neuron dbp

(Lloyd and Sakonju, 1991)

abdominal dorsal multidendritic neuron

(Lloyd and Sakonju, 1991)

dorsal thoracic bipolar dendrite neuron precursor cell

(Lloyd and Sakonju, 1991)

embryonic stage 13

larval MP1 neuron

(Wheeler et al., 2006)

larval H-cell

(Wheeler et al., 2006)

larval H-cell sib

(Wheeler et al., 2006)

(Ng et al., 1995)

(Ng et al., 1995)

(Ng et al., 1995)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 11

Comment: reference states 0-6 hr AEL

(Billin et al., 1991)

embryonic stage 1 -- 15

Comment: reference states 0-12 hr AEL

(Lloyd and Sakonju, 1991)

embryonic stage 7 -- 17

organism

(Billin et al., 1991)

larval stage & pupal stage

(Billin et al., 1991)

larval stage -- adult stage

(Lloyd and Sakonju, 1991)

third instar larval stage

(Lloyd and Sakonju, 1991)

Additional Descriptive Data

Expression assayed at stages 9, 11, 13, and 17. Expression may be continuous between assayed stages in some tissues.

(Wheeler et al., 2006)

expressed first in SOP of this lineage expression subsequently decreases in presumptive glial cell, is re-established once gcm expression has been established, and then disappears by stage 16

(Umesono et al., 2002)

Marker for

larval H-cell

(Stagg et al., 2011)

larval H-cell sib

(Stagg et al., 2011)

larval MP1 neuron

(Stagg et al., 2011)

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 8 -- 11

thoracic neuroblast NB5-6

(Karlsson et al., 2010)

abdominal neuroblast NB5-6

(Karlsson et al., 2010)

embryonic stage 9

ganglion mother cell

(Karlsson et al., 2010)

embryonic stage 9 -- 11

tritocerebral neuroblast | subset

(Urbach and Technau, 2003)

procephalic neurogenic region | restricted

(Urbach and Technau, 2003)

protocerebral neuroblast | subset

(Urbach and Technau, 2003)

deutocerebral neuroblast | subset

(Urbach and Technau, 2003)

embryonic stage 11

ganglion mother cell GMC4-2a

(Yeo et al., 1995)

embryonic stage 16

abdominal dorsal multidendritic neuron ddaE | subset

• nucleus

Comment: reference states dda1

(Brewster et al., 2001)

abdominal dorsal bipolar neuron dbp

• nucleus

Comment: reference states dbd

(Brewster et al., 2001)

scolopidial ligament cell of abdominal lateral pentascolopidial chordotonal organ lch5

• nucleus

(Brewster et al., 2001)

embryonic stage 17

EW2 neuron

(Lundell et al., 2003, Lundell and Hirsh, 1998)

first instar larval stage

larval lateral horn Leucokinin neuron

(Herrero et al., 2014)

second instar larval stage

wing pouch

Comment: area that corresponds to distal region of wing

(Terriente et al., 2008)

third instar larval stage -- prepupal stage P4

presumptive coxa

(Greenberg and Hatini, 2009)

presumptive trochanter

(Greenberg and Hatini, 2009)

presumptive femur

(Greenberg and Hatini, 2009)

presumptive tibia

(Greenberg and Hatini, 2009)

presumptive metatarsus

(Greenberg and Hatini, 2009)

early third instar larval stage

presumptive tarsal segment 3 -- presumptive tarsal segment 5

(Natori et al., 2012)

pretarsus

(Natori et al., 2012)

late third instar larval stage

leg joint

(Natori et al., 2012)

presumptive tarsal segment 5

(Natori et al., 2012)

wandering third instar larval stage

wing pouch

(Nusinow et al., 2008)

wing hinge primordium | distal

(Nusinow et al., 2008)

prepupal stage P3 -- P4

leg joint

(Turchyn et al., 2011)

pupal stage

leg joint | proximal | subset

(Mirth and Akam, 2002)

adult stage

adult fat body

(Dantoft et al., 2013)

adult midgut

(Dantoft et al., 2013)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood | male -- day 7 of adulthood | male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Antibody used is specific to the nub-PB and nub-PD epitopes.

(Dantoft et al., 2013)

nub marks the wing pouch and dorsal hinge.

(Hatini et al., 2013)

Early in third instar, nub is expressed in a broad region from the pretarsus through the presumptive tarsal segments, up to the distal part of the dac domain in presumptive tarsal segment 2. By late third instar, nub expression disappears from the intersegmental region of true segments and the pretarsal region, remaining only in the joint-forming cells.

(Natori et al., 2012)

Between 4 and 12 hr APF, nub expression is proximally adjacent to EcollacZ^odd-rK111, which labels the proximal parts of all leg joints except those between tarsal subsegments.

(Turchyn et al., 2011)

nub protein is localized to proximal half of the true leg segments of leg discs; localization is proximal to that of bowl and TfAP-2.

(Greenberg and Hatini, 2009)

tsh protein is expressed in the wing pouch and distal wing hinge, but not in the presumptive notum. Expression is mutually exclusive with that of tsh.

(Nusinow et al., 2008)

Expression first seen in the wing disc during middle to late second instar in an area that corresponds to the distal region of the wing. The nub domain is a bit broader than the rn domain.

(Terriente et al., 2008)

Markers that uniquely identify the cells of the NB3-7 lineage were used to examine the serotonin expressing cell lineages.

(Lundell et al., 2003)

Expression in procephalic neuroblasts stage 9-11: tritocerebrum - d1-6, v1, v5; deuterocerebrum - d1-8, v2-8; protocerebrum - ad1, ad2, ad4-9, ad12, ad15, ad17, av1, cd1-6, cd8-14, cd17, cd20, cd21, cv1, cv3-9, pd2, pd4, pd5, pd8, pd9, pd12, pd16, pv1-3

(Urbach and Technau, 2003)

Protein is detected in ring pattern in proximal joint of third instar larval leg discs. At 34-38 hours after pupal formation protein is expressed in the tibial and not the tarsal leg joints in the proximal joint tissue and a subset of bristle lineage.

(Mirth and Akam, 2002)

nub protein is expressed in the wing pouch region of the third instar wing disc.

(Wu and Cohen, 2002)

nub is expressed in the lateral, but not the medial, of the paired ventro-lateral serotonergic neurons.

(Lundell and Hirsh, 1998)

nub is expressed in early stages of the developing CNS in a highly dynamic pattern that includes roughly half of the neuroblasts and several ganglion mother cells. Here, nub and pdm2 are shown to be coexpressed in GMC4-2a but not in its progenitor neuroblast or its mature progeny.

(Yeo et al., 1995)

Marker for

adult enterocyte

(Beebe et al., 2010)

enterocyte

(Bardin et al., 2010)

external sensory organ precursor cell IIa

(Ayeni et al., 2016)

wing pouch

(Hatini et al., 2013)

Subcellular Localization

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Brewster et al., 2001)

Expression Deduced from Reporters

Reporter: P{GawB}nub-AC-62

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

leg disc | restricted

(Azpiazu and Morata, 2002)

haltere disc | restricted

(Estella et al., 2003)

wing | presumptive

(Barrio and de Celis, 2004, del Alamo Rodriguez et al., 2004, Estella et al., 2003)

wing | presumptive | restricted

(Calleja et al., 2000)

wing disc | restricted

(Barrio and de Celis, 2004, del Alamo Rodriguez et al., 2004, Estella et al., 2003, Calleja et al., 2000)

third instar larval stage

wing disc | restricted

(Azpiazu and Morata, 2000)

wing | presumptive | restricted

(Azpiazu and Morata, 2000)

wandering third instar larval stage

wing pouch

(Peterson and O'Connor, 2013, Tortoriello et al., 2010)

wing hinge primordium

(Tortoriello et al., 2010)

Reporter: P{nub-GAL4.K}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

ganglion mother cell

(Kuzin et al., 2005)

neuron

(Kuzin et al., 2005)

neuroblast

(Kuzin et al., 2005, Koizumi et al., 2001)

embryonic stage 9 -- 10

ventral nerve cord primordium | restricted

(Kambadur et al., 1998)

cell of ventral nerve cord primordium

(Kambadur et al., 1998)

embryonic stage 10 -- 12

neuroblast | subset

(Kambadur et al., 1998)

ganglion mother cell | subset

(Kambadur et al., 1998)

wandering third instar larval stage

wing pouch

(Csordás et al., 2020)

prepupal stage P1 -- pupal stage P6

wing blade

(Iyer et al., 2019)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\nub in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\nub

View exonic expression by tissue for Dmel\nub

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0085424

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34669/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0085424

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0085424

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0085424

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6246

Flygut - An atlas of the Drosophila adult midgut

FBgn0085424

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\nub

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 20 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 27 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of nub

Transgenic constructs containing regulatory region of nub

Aberrations (Deficiencies and Duplications) ( 13 )

Inferred from experimentation ( 13 )

Gene not disrupted in

Df(2L)BSC30

(Deal and Cook, 2003.4.29)

Df(2L)pdm2^GR19

(Yeo et al., 1995)

Gene duplicated in

Dp(2;2)GYL

(Bhat and Schedl, 1994)

Gene partially disrupted in

Df(2L)nub¹

(Loker and Mann, 2022)

Df(2L)nub-NPW-large

(Loker and Mann, 2022)

Df(2L)nub-NPW-small

(Loker and Mann, 2022)

Df(2L)Prl

(Yamamoto et al., 2009.2.25)

Inferred from location ( 12 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

nub^R5

lethal | male limited | cold sensitive, with Scer\GAL4^Bx-MS1096

nub^{UAS.ORF.GW.Tag:HA}

lethal - all die before end of P-stage (with Df(2L)GR4)

nub^E37

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

nub^GD9039

partially lethal | male limited, with Scer\GAL4^Bx-MS1096

nub^{UAS.ORF.GW.Tag:HA}

partially lethal - majority die, with Scer\GAL4^pnr-MD237

nub^GD9038

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

nub^GD1498

viable, with Scer\GAL4^tin.CΔ4

nub^GD1498

Sterility

Allele

fertile

nub^62d

Other Phenotypes

Allele

abnormal neuroanatomy

nub²

abnormal neuroanatomy, with Scer\GAL4^ppk.1.9

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^ppk.PU

nub^HMC03992

abnormal size | adult stage A1

nub¹

increased occurrence of cell division | embryonic stage 16, with Scer\GAL4^elav-C155

nub^{UAS.ORF.GW.Tag:HA}

increased occurrence of cell division | embryonic stage 16, with Scer\GAL4^lbe.K

nub^{UAS.ORF.GW.Tag:HA}

some die during pharate adult stage (with Df(2L)GR4)

nub^E37

some die during pupal stage, with Scer\GAL4^pnr-MD237

suppressor of variegation | dominant, with P{SUPor-P}KV00108

nub^E37

visible

visible, with Scer\GAL4^Bx-MS1096

nub^KK113120

visible, with Scer\GAL4^pnr-MD237

nub^GD1498

visible | adult stage A1

nub¹

visible | dominant

nub^Prl

visible | female limited, with Scer\GAL4^Bx-MS1096

nub^{UAS.ORF.GW.Tag:HA}

visible | female limited | cold sensitive, with Scer\GAL4^Bx-MS1096

nub^{UAS.ORF.GW.Tag:HA}

Phenotype manifest in

Allele

adult antennal lobe projection neuron DC3 adPN

nub²

adult antennal lobe projection neuron VA2 adPN

nub²

adult antennal lobe projection neuron VM2 adPN

nub²

adult olfactory receptor neuron

nub²

adult olfactory receptor neuron Or42a

nub²

adult olfactory receptor neuron Or43b

nub²

adult olfactory receptor neuron Or83c

nub²

adult olfactory receptor neuron Or85a

nub²

adult olfactory receptor neuron Or92a

nub²

antennal lobe glomerulus DA4m

nub²

antennal lobe glomerulus DC3

nub²

antennal lobe glomerulus DL2

nub²

antennal lobe glomerulus DM5

nub²

antennal lobe glomerulus DM6

nub²

antennal lobe glomerulus DP1l

nub²

antennal lobe glomerulus VA1

nub²

antennal lobe glomerulus VA1v

nub²

antennal lobe glomerulus VA2

nub²

antennal lobe glomerulus VA7l

nub²

antennal lobe glomerulus VA7m

nub²

antennal lobe glomerulus VL2a

nub²

antennal lobe glomerulus VL2p

nub²

antennal lobe glomerulus VM5

nub²

antennal lobe glomerulus VM5v

nub²

antennal lobe glomerulus VM7

nub²

anterior notal wing process

cephalopharyngeal skeleton

nub^hs.PC

chaeta | increased number, with Scer\GAL4^pnr-MD237

nub^GD1498

crossvein

dorsal row of triple row of wing sensilla

embryonic ganglion mother cell | embryonic stage 16, with Scer\GAL4^elav-C155

nub^{UAS.ORF.GW.Tag:HA}

embryonic ganglion mother cell | embryonic stage 16, with Scer\GAL4^lbe.K

nub^{UAS.ORF.GW.Tag:HA}

embryonic neuroblast | embryonic stage 16, with Scer\GAL4^elav-C155

nub^{UAS.ORF.GW.Tag:HA}

embryonic neuroblast | embryonic stage 16, with Scer\GAL4^lbe.K

nub^{UAS.ORF.GW.Tag:HA}

ganglion mother cell | ectopic, with Scer\GAL4^ftz.ng

nub^UAS.cNa

ganglion mother cell GMC4-2a

nub^E37

haltere

larval abdominal segment 1

nub^hs.PC

larval abdominal segment 2

nub^hs.PC

larval dorsal multidendritic neuron ddaC, with Scer\GAL4^ppk.1.9

larval head

nub^hs.PC

larval mesothoracic segment

nub^hs.PC

larval metathoracic segment

nub^hs.PC

larval multidendritic class IV neuron | third instar larval stage, with Scer\GAL4^ppk.PU

nub^HMC03992

larval RP2 motor neuron

larval RP2 motor neuron | ectopic, with Scer\GAL4^ftz.ng

nub^UAS.cNa

larval VL3/4 motor neuron

nub^hs.P

larval VO1 motor neuron

nub^hs.P

larval VO2 motor neuron

nub^hs.P

leg

nub¹

medial row of triple row of wing sensilla

metatarsus of male prothoracic leg (with Df(2L)GR4)

nub^E37

neuroblast NB4-2 | ectopic, with Scer\GAL4^ftz.ng

nub^UAS.cNa

olfactory receptor neuron

nub²

pleural wing process

posterior notal wing process

sensory mother cell | ectopic

nub¹

sex comb | ectopic (with Df(2L)GR4)

nub^E37

tarsal segment (with Df(2L)GR4)

nub^E37

tibia (with Df(2L)GR4)

nub^E37

ventral row of triple row of wing sensilla

wing

wing, with Scer\GAL4^Bx-MS1096

wing | female limited | cold sensitive, with Scer\GAL4^Bx-MS1096

nub^{UAS.ORF.GW.Tag:HA}

wing disc

wing hinge

wing margin bristle

nub¹

wing margin bristle | ectopic

nub¹

wing margin bristle | ectopic | somatic clone

nub¹

wing vein L2

nub¹

wing vein L4

wing vein L5

nub¹

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (84)

Hsap\POU2F2

8 of 14

Yes

Hsap\POU2F1

7 of 14

Yes

Hsap\POU2F3

6 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\DUX4

1 of 14

Hsap\DUX4L4

1 of 14

Yes

Hsap\DUX4L7

1 of 14

Yes

Hsap\DUX4L9

1 of 14

Yes

Hsap\DUX5

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\HESX1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (25)

Rnor\Pou2f1

7 of 14

Yes

Rnor\Pou2f2

7 of 14

Yes

Rnor\Pou2f3

6 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Rnor\Dbx1

1 of 14

Rnor\Hdx

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (25)

Mmus\Pou2f2

8 of 14

Yes

Mmus\Pou2f1

7 of 14

Yes

Mmus\Pou2f3

6 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Mmus\Hdx

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (69)

Xtro\pou2f2

7 of 13

Yes

Xtro\pou2f1

6 of 13

Yes

Xtro\pou2f3

4 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\hesx1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (32)

Drer\pou2f2a

7 of 14

Yes

Drer\pou2f1b

5 of 14

Yes

Drer\pou2f3

5 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (30)

Cele\ceh-18

3 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (33)

Agam\AgaP_AGAP009500

10 of 12

Yes

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (9)

Atha\ANL2

1 of 13

Yes

Atha\ATML1

1 of 13

Yes

Atha\FWA

1 of 13

Yes

Atha\HAT2

1 of 13

Yes

Atha\HB4

1 of 13

Yes

Atha\HB5

1 of 13

Yes

Atha\HB21

1 of 13

Yes

Atha\HDG1

1 of 13

Yes

Atha\PDF2

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\PHO2

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:nub. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (32)

pdm2

7 of 13

vvl

5 of 13

4 of 13

3 of 13

1 of 13

1 of 13

bsh

1 of 13

C15

1 of 13

CG9876

1 of 13

Dbx

1 of 13

Dfd

1 of 13

Drgx

1 of 13

exd

1 of 13

exex

1 of 13

ftz

1 of 13

gsb

1 of 13

gsb-n

1 of 13

HHEX

1 of 13

ind

1 of 13

lbe

1 of 13

lbl

1 of 13

lms

1 of 13

Ptx1

1 of 13

Scr

1 of 13

slou

1 of 13

tin

1 of 13

toe

1 of 13

zen2

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

nub¹

model of immune system disease

CEA

(Dantoft et al., 2013)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

nub^UAS.cUa

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q70.cc0.UAS.Tag:HA,Tag:MYC}

(Kwon et al., 2018)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

POU2F2; POU class 2 homeobox 2

8 of 14

164176

POU2F1; POU class 2 homeobox 1

7 of 14

164175

POU2F3; POU class 2 homeobox 3

6 of 14

607394

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

RNA-protein

Physical Interaction

Assay

References

nub - stau

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

protein-protein

Physical Interaction

Assay

References

(Ma et al., 2000)

(Shokri et al., 2019)

nub - pdm2

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

nub - pinta

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

nub - wek

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

nub

suppressible

(Neumann and Cohen, 1998)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

nub

(Bronstein et al., 2010)

enhanceable

nub

(Garoia et al., 2000)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60715

DroID - A comprehensive database of gene and protein interactions.

FBgn0085424

MIST (protein-protein) - An integrated Molecular Interaction Database

34669

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P31368

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-47

Cytogenetic map

33F1-33F2

Sequence location

2L:12,587,625..12,628,135 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

33F1-33F2

Limits computationally determined from genome sequence between P{lacW}bun^k00612&P{EP}EP682^EP682 and P{lacW}l(2)k07015^k07015&P{PZ}l(2)rK639^rK639

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

33F-33F

(determined by in situ hybridisation)

(Billin et al., 1991, Dick et al., 1991)

33F1-33F2

(determined by in situ hybridisation)

(Lloyd and Sakonju, 1991)

Experimentally Determined Recombination Data

Location

2-47

(FlyBase, 2016, Seiger, 1963)

2-41.8

(Comeron et al., 2012)

2-47.0

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (92)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

86108

w^*; P{nub-GAL4.K}2

358

w¹¹¹⁸; nub²

8856

nub^E37/CyO, P{ftz-lacB}E3

FlyORF

F004763

M{UAS-nub.ORF-VN}ZH-86Fb

F000147

M{UAS-nub.ORF.3xHA.GW}ZH-86Fb

F004854

M{UAS-nub.ORF-VNshort}ZH-86Fb

Korea

13367

P{VT006426-GAL4}attP2

15001

P{VT006448-GAL4}attP2

15578

P{GMR78A04-GAL4}attP2

105872

v105044

v107814

More stocks available...

Genomic Clones (36)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (42)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE34782

Other clones

DPiM_RE34782

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0085424

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0085424

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Dai et al., 2020, Dantoft et al., 2013, Lee et al., 2010, Terriente et al., 2008, Averof and Cohen, 1997, Yeo et al., 1995)

Commercially Available Antibodies

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

Nub_2D4

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

nub/pdm2 and cas regulate late-born motor neuron identity within the NB7-1 lineage; nub/pdm2 specifies fourth-born U4 motor neuron identity, while nub/pdm2/cas together specify fifth-born U5 motor neuron identity.

(Grosskortenhaus et al., 2006)

The embryonic CNS contains sequentially generated neuroblast sublineages that can be distinguished by their expression of either hb, nub or cas. hb and cas may directly silence nub expression in early and late developing sublineages, given that nub cis-regulatory DNA contains approximately 32 hb/cas-binding sites and its enhancer(s) are ectopically activated in cas^- neuroblasts. Targeted misexpression of cas in all neuronal lineages reduces nub expression without altering hb expression. By ensuring correct POU gene expression boundaries hb and cas maintain temporal subdivisions in the cell-identity circuitry controlling CNS development.

(Kambadur et al., 1998)

The POU domain protein encoded by nub contributes to the formation of a sharp dorsoventral boundary in the wing.

(Neumann and Cohen, 1998)

The expression patterns of crustacean homologues of nub and ap support the hypothesis that insect wings evolved from gill-like appendages that were present in the aquatic ancestors of both crustaceans and insects.

(Averof and Cohen, 1997)

nub gene is important for correct proximal-distal specification of the growing wing imaginal disc.

(Cifuentes and Garcia-Bellido, 1997)

Incorrect specification of the proximal-distal axis of the growing wing imaginal disc due to mutations in nub leads to failures in growth along both the D-V and A-P axis.

(Cifuentes and Garcia-Bellido, 1997)

Isolated from a Drosophila 10-22 hour embryo cDNA expression library, using a 22bp sequence from the Cha upstream regulatory region as a probe. This 22bp sequence has been shown to be bound by specific binding factor(s) in embryo nuclear extracts.

(Kitamoto and Salvaterra, 1995)

nub mutants do not interact genetically with mutations in ap, Bx, ct, sens, N, sd, Ser, wg, vg, wg. The mutant wing phenotype is distinct from those of ap, vg, sd and fu.

(Ng et al., 1995)

nub is required for the normal growth and patterning of the wing. Mosaic analysis reveals a localized requirement for nub activity in the wing hinge.

(Ng et al., 1995)

Within the hierarchy of genes expressed in GMC4-2a nub and pdm2 lie downstream of pros and ftz and upstream of eve.

(Yeo et al., 1995)

nub and pdm2 both contribute toward the development of a full complement of mature RP2 neurons by being expressed in and acting on ganglion mother cell 2a (GMC-2a), the RP2 progenitor cell. nub plays a quantitatively less important role than pdm2. nub and pdm2 act to specify GMC-2a cell identity and not merely act to positively regulate GMC-2a cell division.

(Yeo et al., 1995)

Proper spatial regulation of nub involves the interaction between the mesoderm and the endoderm, but uses a different genetic cascade than that regulating lab : dpp is not the signal that dictates central nub repression across germ layers.

(Affolter et al., 1993)

One of the homeodomain loci identified in a screen for genes encoding DNA binding proteins capable of binding to a consensus en binding site.

(Kalionis and O'Farrell, 1993)

nub is transcribed in elements of the developing nervous system suggesting a functional role in neurogenesis and neurospecification.

(Dick et al., 1992)

nub contains a POU domain, and is expressed at maximum levels during early development.

(Billin et al., 1991)

Relationship to Other Genes

Source for database merge of

Source for merge of: nub CG15488 CG15489

(FlyBase Genome Annotators, 2007, Jacobsen et al., 2006)

Additional comments

Annotations CG6246, CG15489 and CG15488 merged as CG34395 in release 5.2 of the genome annotation.

(FlyBase Genome Annotators, 2007)

Nomenclature History

Source for database identify of

Source for identity of: nub CG6246

(Zhong, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (32)

Reported As

Symbol Synonym

CG15488

(FlyBase Genome Annotators, 2007, Jacobsen et al., 2006)

CG15489

(Bronstein et al., 2010, Ryuda et al., 2008, FlyBase Genome Annotators, 2007, Jacobsen et al., 2006)

CG34395

(National Institute of Genetics Fly Stocks, 2022-, Hadar et al., 2012, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, FlyBase Genome Annotators, 2007)

CG6246

(Perkins et al., 2009, Kreiman, 2004, Poole, 2000.4.14)

NUB

(Riahi et al., 2021, Samee and Sinha, 2013, Cho et al., 2006)

(Herrera et al., 2013, Smith-Bolton et al., 2009)

Nubbin

(Nässel, 2021, Tian and Smith-Bolton, 2021)

Oct-1

(Lee et al., 2010, Malin et al., 2005, Gehring et al., 1994)

Oct1

(Malin et al., 2005)

PDM-1

(Jiang et al., 2011)

PDM1

(Li et al., 2013, Shaw et al., 2010, Fichelson and Gho, 2004)

POU33F1

(Affolter et al., 1992)

Pdm

(Lopes et al., 2020, Reichert, 2017, Jiang et al., 2016, Pinto-Teixeira et al., 2016, Yasugi and Nishimura, 2016, Takashima et al., 2011, Beebe et al., 2010, Kao and Lee, 2010, Sawa, 2010, Sousa-Nunes et al., 2010, Doe, 2006, Grosskortenhaus et al., 2006, Jones, 2005, Cenci et al., 2003, Skeath and Thor, 2003, Zhong, 2003, Pasquinelli and Ruvkun, 2002, Kabbarah et al., 1998)

Pdm-1

(Takashima et al., 2011, Lee et al., 2010, Novotny et al., 2002, Brody and Odenwald, 2000, Ma et al., 2000, Brody and Odenwald, 1999, Brody and Odenwald, 1999, Brody and Odnewald, 1999, Odenwald et al., 1999, Buescher et al., 1998)

Pdm1

(Gujar and Wang, 2025, Veneti et al., 2024, Wang et al., 2024, Zhao et al., 2024, Pollington et al., 2023, Stączek et al., 2023, Darnat et al., 2022, Simon and Konstantinides, 2021, Dai et al., 2020, Ding et al., 2020, Hao et al., 2020, Jin et al., 2020, Khaminets et al., 2020, Maurange, 2020, Yaghmaeian Salmani and Thor, 2020, Flint Brodsly et al., 2019, Ramon-Cañellas et al., 2019, Tang and Engström, 2019, Xu et al., 2019, Lan et al., 2018, Xu et al., 2017, Corty et al., 2016, Li and Jasper, 2016, Kohlmaier et al., 2015, Zhai et al., 2015, Li et al., 2014, Guo et al., 2013, He et al., 2013, Homem and Knoblich, 2012, Kapuria et al., 2012, Kelsom and Lu, 2012, Poernbacher et al., 2012, Apidianakis and Rahme, 2011, Bhat et al., 2011, Perdigoto et al., 2011, Simons and Clevers, 2011, Mathur et al., 2010, Mathur et al., 2010, Ren et al., 2010, Lee et al., 2009, Southall and Brand, 2009, Cleary and Doe, 2006, Doe et al., 2005, Fichelson et al., 2005, Orgogozo and Grueber, 2005, Stollewerk and Simpson, 2005, Lundell et al., 2003, McDonald et al., 2003, Isshiki et al., 2001, Isshiki et al., 2001, Koizumi et al., 2001, Cheah et al., 2000)

Pdm1/nubbin

(Kong et al., 2021)

dOct1

(Prakash et al., 1992)

dPOU-19

(Goodman and Doe, 1993, Cabrera, 1992, Dick et al., 1991)

dPOU-19/pdm-1

(Kitamoto and Salvaterra, 1995)

dPOU19

(Yeo et al., 1995, Dick et al., 1992)

(Endo et al., 2007, de Celis, 2003, Gibert et al., 2002)

nub

(Cruz et al., 2024, Dong et al., 2023, Floc'hlay et al., 2023, McQuarrie et al., 2023, Loker and Mann, 2022, Ray et al., 2022, Velten et al., 2022, Everetts et al., 2021, Kong et al., 2021, Loker et al., 2021, Mahmud et al., 2020, Nystrom et al., 2020, Port et al., 2020, Ahmad and Spens, 2019, Belmonte et al., 2019, Oberst et al., 2019, Sapar and Han, 2019, Shokri et al., 2019, Villanueva-Cañas et al., 2019, Aughey et al., 2018, Bischof et al., 2018, Tang et al., 2018, Croze et al., 2017, Karaiskos et al., 2017, Kojima, 2017, Transgenic RNAi Project members, 2017-, Bürglin and Affolter, 2016, Dantoft et al., 2016, Gabilondo et al., 2016, Duque and Sinha, 2015, Schertel et al., 2015, Ghavi-Helm et al., 2014, Marr et al., 2014, Neville et al., 2014, Shukla et al., 2014, Aleksic et al., 2013, Combs and Eisen, 2013, Dantoft et al., 2013, Herrera et al., 2013, Iyer et al., 2013, Li and Gilmour, 2013, Perea et al., 2013, Crombach et al., 2012, Hadar et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Kao et al., 2012, Kim et al., 2012, Nfonsam et al., 2012, Zhou et al., 2012, Bellen et al., 2011, Lee et al., 2011, Liu et al., 2011, Matta et al., 2011, Schrider et al., 2011, Turchyn et al., 2011, Karlsson et al., 2010, Kazemian et al., 2010, Losada-Pérez et al., 2010, Wasbrough et al., 2010, Dichtel-Danjoy et al., 2009, Fang et al., 2009, Perea et al., 2009, Yamamoto et al., 2009.2.25, Segal et al., 2008, Takacs et al., 2008, Terriente et al., 2008, Yu and Small, 2008, Barrio et al., 2007, Ciechanska et al., 2007, Hueber et al., 2007, Zirin and Mann, 2007, Molnar and de Celis, 2006, Wheeler et al., 2006, Morrow et al., 2005, Berman et al., 2004, Grad et al., 2004, Kreiman, 2004, Averof and Cohen, 1997)

pdm

(Myasnikova and Spirov, 2020, Herrero et al., 2014, Nakajima et al., 2010, Tsuji et al., 2008, Tran and Doe, 2007, Grosskortenhaus et al., 2005, Odenwald, 2005, Abbott, 2003, Kanai et al., 2003, Pearson and Doe, 2003, Bellaiche and Schweisguth, 2001, Harris, 2001, Livesey and Cepko, 2001, Jockusch and Nagy, 1997)

twn

Name Synonyms

CG15488

CG15489

NUBBIN

(Samee and Sinha, 2013)

POU domain 1

(Herrero et al., 2014)

POU domain protein 1

(Li and Jasper, 2016, Homem and Knoblich, 2012, Brody et al., 2002)

Pdm1

(Zhou et al., 2021, Zhai et al., 2018)

twain

Secondary FlyBase IDs

FBgn0032438
FBan0015488
FBgn0002970
FBan0006246
FBgn0032440
FBgn0004393

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 61 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34669

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P31368

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P31368

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P31368

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0085424

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34669/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0085424

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0085424

FlyMine - An integrated database for Drosophila genomics

FBgn0085424

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG34395

MARRVEL_MODEL - MARRVEL (model organism gene)

34669

Linkouts

BioGRID - A database of protein and genetic interactions.

60715

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0085424

DroID - A comprehensive database of gene and protein interactions.

FBgn0085424

DRSC - Results frm RNAi screens

FBgn0085424

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

Nub_2D4

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0085424

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0085424

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6246

Flygut - An atlas of the Drosophila adult midgut

FBgn0085424

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0085424

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC032751

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

34669

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P31368

References (458)

The following external sites may use different assemblies or annotations than FlyBase.

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