FlyBase Gene Report: Dmel\DAT

General Information

Symbol

Dmel\DAT

Species

D. melanogaster

Name

Dopamine transporter

Annotation Symbol

CG8380

Feature Type

protein_coding_gene

FlyBase ID

FBgn0034136

Gene Model Status

Current

Stock Availability

63 publicly available

Gene Summary

Dopamine transporter (DAT) encodes a dopamine transporter that mediates uptake of dopamine from the synaptic cleft. Its loss increases extracellular dopamine and is associated with behavioral phenotypes including increased activity and decreased sleep. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Also Known As

dDAT, fumin, fmn, DmDAT

Key Links

Genomic Location

Cytogenetic map

53C7-53C8

Sequence location

2R:16,558,567..16,565,238 [-]

Recombination map

2-80

RefSeq locus

NT_033778 REGION:16558567..16565238

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (17 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables cocaine binding

inferred from direct assay

(Porzgen et al., 2001)

enables dopamine:sodium symporter activity

inferred from direct assay

(Wu and Gu, 2003)

inferred from direct assay

(Porzgen et al., 2001)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables dopamine:sodium symporter activity

inferred from biological aspect of ancestor with PANTHER:PTN000746001

(GO Reference Genome Project, 2011-)

Biological Process (10 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in adult locomotory behavior

inferred from mutant phenotype

(van der Voet et al., 2016)

involved_in circadian sleep/wake cycle

inferred from mutant phenotype

(Ueno and Kume, 2014)

inferred from direct assay

(Kume et al., 2005)

involved_in dopamine transport

inferred from direct assay

(Porzgen et al., 2001)

inferred from direct assay

(Wu and Gu, 2003)

involved_in regulation of presynaptic cytosolic calcium ion concentration

inferred from mutant phenotype

(Cohn et al., 2015)

involved_in response to odorant

inferred from mutant phenotype

(Cohn et al., 2015)

involved_in sleep

inferred from mutant phenotype

(Wu et al., 2008)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in amino acid transport

inferred from biological aspect of ancestor with PANTHER:PTN000907004

(GO Reference Genome Project, 2011-)

involved_in dopamine uptake involved in synaptic transmission

inferred from biological aspect of ancestor with PANTHER:PTN000746001

(GO Reference Genome Project, 2011-)

involved_in norepinephrine transport

inferred from biological aspect of ancestor with PANTHER:PTN000746001

(GO Reference Genome Project, 2011-)

involved_in sodium ion transmembrane transport

inferred from biological aspect of ancestor with PANTHER:PTN000177662

(GO Reference Genome Project, 2011-)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in plasma membrane

inferred from direct assay

(Wang et al., 2015, Penmatsa et al., 2013)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

is_active_in axon

inferred from biological aspect of ancestor with PANTHER:PTN000746001

(GO Reference Genome Project, 2011-)

located_in membrane

inferred from electronic annotation with InterPro:IPR000175

(InterPro Project Members, 2004-)

is_active_in neuronal cell body membrane

inferred from biological aspect of ancestor with PANTHER:PTN000746001

(GO Reference Genome Project, 2011-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000177662

(GO Reference Genome Project, 2011-)

located_in plasma membrane

inferred from sequence model

(Porzgen et al., 2001)

is_active_in presynaptic membrane

inferred from biological aspect of ancestor with PANTHER:PTN000746001

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

SLC6 FAMILY OF SODIUM/CHLORIDE-DEPENDENT NEUROTRANSMITTER TRANSPORTERS

Protein Family (UniProt)

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. (Q7K4Y6)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

dopamine:sodium symporter activity

Summaries

Gene Snapshot

Gene Group (FlyBase)

SLC6 FAMILY OF SODIUM/CHLORIDE-DEPENDENT NEUROTRANSMITTER TRANSPORTERS -: Solute carrier family 6 (SLC6) members are Na⁺/Cl^--dependent transporters of extracellular neurotransmitters, amino acids and osmolytes across the plasma membrane into the cytosol. (Adapted from FBrf0193583).

Protein Function (UniProtKB)

Sodium-dependent dopamine transporter which terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals (PubMed:11125028, PubMed:12606774, PubMed:24037379, PubMed:25970245). Also transports tyramine and norepinephrine, shows less efficient transport of octopamine and does not transport serotonin (PubMed:11125028, PubMed:12606774). Plays a role in the regulation of the rest/activity cycle (PubMed:16093388, PubMed:25232310).

(UniProt, Q7K4Y6)

Summary (Interactive Fly)

mediates uptake of dopamine in dopamine positive neurons - functions in regulating sleep and arousal

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\DAT for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q7K4Y6)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

Comments on Gene Model

Stop-codon suppression (UGA) postulated; FBrf0216884

Gene model reviewed during 5.44

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

DAT-RA

FBtr0087125

NM_079039

3040

631

DAT-RB

FBtr0330002

NM_001274097

3040

644

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

DAT-PA

FBpp0086271

70.3

631

6.31

FBpp0303037

71.9

644

6.70

A0A0B4KEX2

NP_001261026

AGB93558

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\DAT using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

DAT_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.68

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 6

central brain anlage in statu nascendi

(Fisher et al., 2012)

posterior ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

yolk nucleus

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic central brain neuron

(Fisher et al., 2012)

larval nervous system

(Fisher et al., 2012)

lateral cord neuron

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

ventral sensory complex primordium

(Fisher et al., 2012)

embryonic stage 14

larval H-cell

(Stagg et al., 2011)

embryonic stage 17

larval H-cell

(Wheeler et al., 2006)

third instar larval stage

dopaminergic neuron

• neuronal cell body

(Porzgen et al., 2001)

dopaminergic neuron

(Porzgen et al., 2001)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

(Romero-Calderon et al., 2007)

adult thorax

Comment: Thorax and abdomen assayed together.

(Romero-Calderon et al., 2007)

adult abdomen

Comment: Thorax and abdomen assayed together.

(Romero-Calderon et al., 2007)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Porzgen et al., 2001)

embryonic stage 1 -- 9

organism | faint

Comment: residual maternal transcript

(Porzgen et al., 2001)

embryonic stage 15 -- 17

Comment: 12-24 hr AEL; not expressed 4-12 hr AEL

(Porzgen et al., 2001)

first instar larval stage -- adult stage

organism

(Porzgen et al., 2001)

adult stage | male

adult head

(Porzgen et al., 2001)

adult thorax & adult abdomen

Comment: reference states "body"

(Porzgen et al., 2001)

adult stage | female

adult head

(Porzgen et al., 2001)

adult thorax | faint & adult abdomen | faint

Comment: reference states "body"

(Porzgen et al., 2001)

Additional Descriptive Data

In situ hybridization in third instar larvae show that DAT is expressed in dopaminergic (DA) neurons. In the brain, it is observed in three DA neuron cell groups in each lobe. In the ventral nerve cord, it is seen in eight unpaired medial DA neurons along the midline and in two rows of seven dorsal lateral DA neurons. A developmental profile done by RT-PCR show robust expression in late embryos and larvae, a dip in expression in pupae and an increase in adults, with heads showing enhanced signal over bodies.

(Porzgen et al., 2001)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in plasma membrane

inferred from direct assay

(Wang et al., 2015, Penmatsa et al., 2013)

Expression Deduced from Reporters

Reporter: P{DAT-B-GAL4}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

dopaminergic PAL neuron | subset

(Xie et al., 2018)

dopaminergic PPL1 neuron | subset

(Xie et al., 2018)

dopaminergic PPL2ab neuron | subset

(Xie et al., 2018)

dopaminergic PPM3 neuron | subset

(Xie et al., 2018)

Reporter: P{DAT-GAL4.L}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

dopaminergic neuron

(Bang et al., 2011)

Reporter: P{GMR55C10-GAL4}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult crepine | faint | restricted