FlyBase Gene Report: Dmel\ihog

General Information

Symbol

Dmel\ihog

Species

D. melanogaster

Name

interference hedgehog

Annotation Symbol

CG9211

Feature Type

protein_coding_gene

FlyBase ID

FBgn0031872

Gene Model Status

Current

Stock Availability

22 publicly available

Gene Summary

interference hedgehog (ihog) encodes a type 1 membrane protein that acts as a co-receptor for the product of hh upstream or at the level of the receptor encoded by ptc. It interacts with the products of disp, dlp, dally and shf. It is involved in imaginal pattern formation and embryonic cuticle pattern formation. [Date last reviewed: 2018-11-15] (FlyBase Gene Snapshot)

All Summaries

Also Known As

CT26314

Key Links

Genomic Location

Cytogenetic map

27C6-27C6

Sequence location

2L:6,945,464..6,948,808 [-]

Recombination map

2-23

RefSeq locus

NT_033779 REGION:6945464..6948808

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (21 terms)

Molecular Function (6 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

enables coreceptor activity

inferred from genetic interaction with FLYBASE:ptc; FB:FBgn0003892

(Yao et al., 2006)

enables hedgehog family protein binding

inferred from physical interaction with FLYBASE:hh; FB:FBgn0004644

(Wu et al., 2019, Zheng et al., 2010, McLellan et al., 2006)

inferred from direct assay

(Yao et al., 2006)

enables heparin binding

inferred from direct assay

(McLellan et al., 2006)

enables patched binding

inferred from physical interaction with FLYBASE:ptc; FB:FBgn0003892

(Zheng et al., 2010)

enables protein binding

inferred from physical interaction with UniProtKB:Q02936

(Yao et al., 2006)

enables protein homodimerization activity

inferred from direct assay

(McLellan et al., 2006)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (10 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

involved_in compound eye development

inferred from genetic interaction with FLYBASE:boi; FB:FBgn0040388

(Camp et al., 2010)

involved_in cuticle pattern formation

inferred from mutant phenotype

(Yao et al., 2006)

inferred from genetic interaction with FLYBASE:boi; FB:FBgn0040388

(Zheng et al., 2010)

involved_in homotypic cell-cell adhesion

inferred from direct assay

(Wu et al., 2019)

involved_in maintenance of protein location in extracellular region

inferred from genetic interaction with FLYBASE:boi; FB:FBgn0040388

(Bilioni et al., 2013)

involved_in segment specification

inferred from mutant phenotype

(Yao et al., 2006)

involved_in smoothened signaling pathway

inferred from genetic interaction with FLYBASE:boi; FB:FBgn0040388

(Camp et al., 2014, Camp et al., 2010, Zheng et al., 2010)

inferred from mutant phenotype

(Yan et al., 2010, Yao et al., 2006)

inferred from genetic interaction with FLYBASE:ptc; FB:FBgn0003892

(Yao et al., 2006)

inferred from genetic interaction with FLYBASE:dlp; FB:FBgn0041604

(Yao et al., 2006)

inferred from direct assay

(McLellan et al., 2006)

involved_in wing disc anterior/posterior pattern formation

inferred from genetic interaction with FLYBASE:boi; FB:FBgn0040388

(Camp et al., 2014)

involved_in wing disc pattern formation

inferred from mutant phenotype

(Yao et al., 2006)

inferred from genetic interaction with FLYBASE:boi; FB:FBgn0040388

(Camp et al., 2010)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in axon guidance

inferred from biological aspect of ancestor with PANTHER:PTN008688141

(GO Reference Genome Project, 2011-)

involved_in cell-cell adhesion

inferred from biological aspect of ancestor with PANTHER:PTN002548291

(GO Reference Genome Project, 2011-)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

located_in cell surface

inferred from direct assay

(Zheng et al., 2010)

located_in cytoneme

inferred from direct assay

(González-Méndez et al., 2017, Bilioni et al., 2013)

located_in membrane

inferred from direct assay

(Yao et al., 2006)

located_in plasma membrane

inferred from direct assay

(Wu et al., 2019)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in axon

inferred from biological aspect of ancestor with PANTHER:PTN008688141

(GO Reference Genome Project, 2011-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN008688141

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Pathway (FlyBase)

HEDGEHOG SIGNALING PATHWAY CORE COMPONENTS

Protein Family (UniProt)

Belongs to the immunoglobulin superfamily. IHOG family. (Q9VM64)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Pathway (FlyBase)

HEDGEHOG SIGNALING PATHWAY CORE COMPONENTS -: The hedgehog signaling pathway is initiated by hedgehog (hh) ligand binding to the extracellular domain of patched receptor (ptc), leading to the derepression of smoothened (smo) activity. Activation of the atypical GPCR smo results in the accumulation of the transcriptional activator form of cubitus interruptus (ci) and the derepression/activation of hh target genes. In the absence of hh, smo is repressed by ptc and ci is processed to a truncated repressor form. (Adapted from FBrf0220683 and FBrf0231236).

Protein Function (UniProtKB)

Mediates response to the active Hedgehog (Hh) protein signal in embryos, functioning upstream or at the level of patched (ptc).

(UniProt, Q9VM64)

Summary (Interactive Fly)

IGcam membrane protein - binds Hedgehog and mediates Hedgehog response

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\ihog for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VM64)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

Comments on Gene Model

Gene model reviewed during 5.46

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

ihog-RA

FBtr0079365

NM_135241

3276

886

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

ihog-PA

FBpp0078993

97.8

886

9.07

Q9VM64

NP_609085

AAF52461

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Homodimer. Heterotetramer; 2 iHog chains bind 2 hh chains when facilitated by heparin, heparin is required to promote high-affinity interactions between hh and iHog.

(UniProt, Q9VM64)

Domain

The first Fibronectin type-III domain mediates a specific interaction with Hh protein, in vitro. The second Fibronectin type-III domain is additionally required for in vivo signaling activity.

(UniProt, Q9VM64)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VM64

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ihog using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ihog_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.42

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

larval foregut atrium | precursor

Comment: reported as atrium primordium

(Fisher et al., 2012)

dorsal epidermis primordium

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

larval foregut atrium

(Fisher et al., 2012)

embryonic anal pad

(Fisher et al., 2012)

embryonic dorsal epidermis

(Fisher et al., 2012)

embryonic epipharynx

(Fisher et al., 2012)

embryonic head sensory system

(Fisher et al., 2012)

embryonic hypopharynx

(Fisher et al., 2012)

third instar larval stage

eye-antennal disc

(Camp et al., 2010)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

wing disc | restricted

(Yan et al., 2010)

Additional Descriptive Data

ihog protein expression is lower in the expression domains of hh and ptc in the wing disc.

(Yan et al., 2010)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cell surface

inferred from direct assay

(Zheng et al., 2010)

located_in cytoneme

inferred from direct assay

(González-Méndez et al., 2017, Bilioni et al., 2013)

located_in membrane

inferred from direct assay

(Yao et al., 2006)

located_in plasma membrane

inferred from direct assay

(Wu et al., 2019)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\ihog in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\ihog

View exonic expression by tissue for Dmel\ihog

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0031872

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33972/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0031872

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0031872

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0031872

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9211

Flygut - An atlas of the Drosophila adult midgut

FBgn0031872

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\ihog

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 13 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 32 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of ihog

Transgenic constructs containing regulatory region of ihog

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene disrupted in

Df(2L)Exel7029

(Ryder, 2004.4.26)

DF(2L)ihog^DC1

(Camp et al., 2010)

Df(2L)iHog^KG

(Yao et al., 2006)

Gene partially disrupted in

Df(2L)ihog^Δ

(Yan et al., 2010)

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal - majority die, with Scer\GAL4^pnr-MD237

ihog^GD108

partially lethal - majority die | rescuable maternal effect

ihog^DC1

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

viable, with Scer\GAL4^tin.CΔ4

viable (with Df(2L)Exel7029)

ihog^DC1

Sterility

Allele

fertile

fertile (with Df(2L)Exel7029)

ihog^DC1

Other Phenotypes

Allele

some die during P-stage | rescuable maternal effect

ihog^DC1

some die during pupal stage, with Scer\GAL4^pnr-MD237

ihog^GD108

visible, with Scer\GAL4^pnr-MD237

ihog^GD108

Phenotype manifest in

Allele

chaeta | increased number, with Scer\GAL4^pnr-MD237

ihog^GD108

cytoneme | P-stage, with Scer\GAL4^hh-Gal4

histoblast nest | P-stage, with Scer\GAL4^hh-Gal4

ventral denticle belt | embryonic stage (with ihog^e02142)

ventral denticle belt | embryonic stage (with ihog^EY)

ihog^e02142

ventral denticle belt | embryonic stage (with ihog^KG)

ihog^e02142

ventral denticle belt | embryonic stage | maternal effect

wing

ihog^KG

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (48)

Hsap\BOC

4 of 14

Yes

Hsap\CDON

4 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\FCRL2

1 of 14

Yes

Hsap\FCRL3

1 of 14

Yes

Hsap\FCRL4

1 of 14

Yes

Hsap\FCRL5

1 of 14

Yes

Hsap\FCRL6

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (36)

Rnor\Boc

3 of 14

Yes

Rnor\Cdon

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Rnor\Igdcc4

2 of 14

Rnor\Neo1

2 of 14

Rnor\Btnl8

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\LOC685767

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Pvr

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (31)

Mmus\Boc

4 of 14

Yes

Mmus\Cdon

3 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Cntn4

1 of 14

Mmus\Cntn5

1 of 14

Mmus\Gm609

1 of 14

Yes

Mmus\Gm17783

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Skint8

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (23)

Xtro\boc

3 of 13

Yes

Xtro\cdon

3 of 13

Yes

Xtro\hmcn1

2 of 13

Yes

Xtro\hmcn2

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\nectin1

1 of 13

Yes

Xtro\nectin4

1 of 13

Yes

Xtro\neo1

1 of 13

Xtro\npffr1.1

1 of 13

Xtro\pecam1

1 of 13

Yes

Xtro\prtg

1 of 13

Xtro\XB5959262

1 of 13

Yes

Danio rerio (Zebrafish) (42)

Drer\cdon

4 of 14

Yes

Drer\boc

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\mypn

1 of 14

Yes

Drer\nectin1a

1 of 14

Drer\nectin1b

1 of 14

Yes

Drer\nectin3a

1 of 14

Drer\nectin4b

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\si:ch73-22o12.1

1 of 14

Drer\si:ch211-57n23.4

1 of 14

Drer\si:ch211-141e20.2

1 of 14

Drer\si:ch211-222f23.6

1 of 14

Drer\si:dkey-31e10.1

1 of 14

Yes

Drer\si:dkey-31e10.5

1 of 14

Yes

Drer\si:dkey-93h22.7

1 of 14

Yes

Drer\zgc:113337

1 of 14

Yes

Drer\zgc:153911

1 of 14

Drer\zgc:172122

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (6)

Cele\him-4

1 of 14

Yes

Cele\lad-2

1 of 14

Yes

Cele\rig-4

1 of 14

Yes

Cele\rig-5

1 of 14

Yes

Cele\sax-3

1 of 14

Yes

Cele\unc-40

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (32)

Agam\AgaP_AGAP002040

7 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:ihog. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (30)

boi

9 of 13

Dscam3

2 of 13

fra

2 of 13

plum

2 of 13

robo2

2 of 13

robo3

2 of 13

sdk

2 of 13

tutl

2 of 13

Ama

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

klg

1 of 13

Lac

1 of 13

Lar

1 of 13

Nrg

1 of 13

robo1

1 of 13

sns

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

CDON; cell adhesion associated, oncogene regulated

model of holoprosencephaly 11

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

BOC; BOC cell adhesion associated, oncogene regulated

4 of 14

608708

CDON; cell adhesion associated, oncogene regulated

4 of 14

608707

HOLOPROSENCEPHALY 11; HPE11

holoprosencephaly 11

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

ihog

Hsap\BOC

(Qi et al., 2023)

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

RNA-RNA

Physical Interaction

Assay

References

ihog - mir-7

quantitative reverse transcription pcr, luminiscence technology, necessary binding region, fluorescence technology

(Da Ros et al., 2013)

protein-protein

Physical Interaction

Assay

References

ihog

cosedimentation, predetermined participant, molecular sieving, anti tag coimmunoprecipitation, anti tag western blot, bimolecular fluorescence complementation, fluorescence microscopy

(Yang et al., 2021, Wu et al., 2019, McLellan et al., 2006)

ihog - Rab8

anti tag coimmunoprecipitation, western blot

(Gore et al., 2021)

ihog - cher

anti tag coimmunoprecipitation, anti tag western blot, Identification by mass spectrometry

(Lalioti et al., 2025)

ihog - dally

colocalization, fluorescence microscopy, inferred by author

(Simon et al., 2021)

ihog - dlp

colocalization, fluorescence microscopy, inferred by author

(Simon et al., 2021)

ihog - mys

anti tag coimmunoprecipitation, anti tag western blot

(Qi et al., 2023)

ihog - ptc

anti bait coimmunoprecipitation, western blot, anti tag western blot

(Yan et al., 2010, Zheng et al., 2010)

ihog - shf

anti tag coimmunoprecipitation, anti tag western blot

(Bilioni et al., 2013)

ihog - vn

enzyme linked immunosorbent assay

(Ozkan et al., 2013)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

ihog

suppressible

boi

(Camp et al., 2010, Zheng et al., 2010)

enhanceable

(Yao et al., 2006)

suppressible

(Qi et al., 2023)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

boi

suppressible

ihog

(Hartman et al., 2010)

Delta

enhanceable

ihog

(Da Ros et al., 2013)

External Data

Subunit Structure (UniProtKB)

Homodimer. Heterotetramer; 2 iHog chains bind 2 hh chains when facilitated by heparin, heparin is required to promote high-affinity interactions between hh and iHog.

(UniProt, Q9VM64 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0031872

MIST (genetic) - An integrated Molecular Interaction Database

33972

MIST (protein-protein) - An integrated Molecular Interaction Database

33972

Pathways

Signaling Pathways (FlyBase)

HEDGEHOG SIGNALING PATHWAY CORE COMPONENTS

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Hedgehog signaling pathway - fly

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9VM64

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-23

Cytogenetic map

27C6-27C6

Sequence location

2L:6,945,464..6,948,808 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

27C6-27C6

Limits computationally determined from genome sequence between P{lacW}xl6^k00230&P{EP}Hrb27C^EP748 and P{PZ}smt3⁰⁴⁸⁴¹&P{PZ}smt3⁰⁴⁴⁹³

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-23

(FlyBase, 2016)

2-22.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (22)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

606968

w^*; P{UAS-ihog.ΔFn1.mRFP1}3

85563

w¹¹¹⁸; PBac{RB}ihog^e02142

28090

y¹ w^*; P{EP}ihog^G13202

606966

w^*; Kr^If-1/CyO; P{UAS-ihog.RFP}3

606974

w^*; P{UAS-ihog.ΔCT.mRFP1}3/TM6B, Tb¹

606971

w^*; P{UAS-ihog.RFP}2/CyO; MKRS/TM6B, Tb¹

606973

P{hsFLP}12 w^*; P{UAS-ihog.ECFP}2; MKRS/TM6B, Tb¹

NIG-Fly

HMJ21803

y¹ v¹; P{TRiP.HMJ21803}attP40/CyO

M2L-2833

y¹ w¹¹¹⁸; ihog^SK2 P{neoFRT}40A/CyO-Tb

M2L-2834

y¹ w¹¹¹⁸; ihog^SK5 P{neoFRT}40A/CyO-Tb

VDRC

v102602

P{KK112149}VIE-260B

v29897

w¹¹¹⁸; P{GD14317}v29897

More stocks available...

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (22)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH03927

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0031872

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0031872

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Gore et al., 2021, Camp et al., 2014)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

iHog and boi are in the same protein family and appear to overlap functionally.

(Yao et al., 2006)

Epistatic experiments show that iHog acts at or upstream of ptc and upstream of smo.

(Yao et al., 2006)

Epistasis experiments suggest that iHog acts upstream or at the level of ptc.

(Yao et al., 2005)

The iHog protein may be a cell surface receptor for the hh protein, possibly acting as a co-receptor with the ptc gene product.

(Yao et al., 2005)

Inactivation of iHog function leads to defects characteristic of loss of hh signaling in embryos and imaginal discs.

(Yao et al., 2005)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: iHog CG9211

(Yao, 2005.3.3)

Nomenclature comments

Etymology

The gene is named "interference Hedgehog"due to the fact that dsRNA against this gene reduces the response to hh signalling.

(Yao et al., 2006)

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

CG9211

(National Institute of Genetics Fly Stocks, 2022-, Hosono et al., 2015, Camp et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Rehwinkel et al., 2006, Yao et al., 2006, Vogel et al., 2003, Jasper et al., 2001)

CT26314

(Lum et al., 2003, Hynes and Zhao, 2000)

IHOG

(Zhang et al., 2021, Ingham et al., 2011)

iHog

(Mbodj et al., 2013, Ozkan et al., 2013, Toku et al., 2011, Pospisilik et al., 2010, Dworkin et al., 2009)

Name Synonyms

Interference Hedgehog

(Tran et al., 2013)

Interference hedgehog

(Avanesov and Blair, 2013, Gao et al., 2013, Callejo et al., 2011, Camp et al., 2010, Chou et al., 2010, Yan et al., 2010, Zheng et al., 2010)

interference Hedgehog

(Ozkan et al., 2013, McLellan et al., 2006, Yao, 2005.3.3)

interference hedgehog

(Gradilla et al., 2014, Da Ros et al., 2013, Wilson and Chuang, 2006, Yao et al., 2006)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 48 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33972

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VM64

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VM64

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VM64

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0031872

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33972/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0031872

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0031872

FlyMine - An integrated database for Drosophila genomics

FBgn0031872

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG9211

MARRVEL_MODEL - MARRVEL (model organism gene)

33972

PDB - An information portal to biological macromolecular structures

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0031872

DroID - A comprehensive database of gene and protein interactions.

FBgn0031872

DRSC - Results frm RNAi screens

FBgn0031872

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ihog_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0031872

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0031872

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9211

Flygut - An atlas of the Drosophila adult midgut

FBgn0031872

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC007250

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Hedgehog signaling pathway - fly

MIST (genetic) - An integrated Molecular Interaction Database

33972

MIST (protein-protein) - An integrated Molecular Interaction Database

33972

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9VM64

References (110)

The following external sites may use different assemblies or annotations than FlyBase.

-0.42

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