FlyBase Gene Report: Dmel\Rfx

General Information

Symbol

Dmel\Rfx

Species

D. melanogaster

Name

Rfx

Annotation Symbol

CG6312

Feature Type

protein_coding_gene

FlyBase ID

FBgn0020379

Gene Model Status

Current

Stock Availability

39 publicly available

Gene Summary

Rfx (Rfx) encodes a transcription factor involved in the differentiation of ciliated sensory neurons. [Date last reviewed: 2019-09-12] (FlyBase Gene Snapshot)

All Summaries

Also Known As

dRFX, Regulatory factor X

Key Links

Genomic Location

Cytogenetic map

86B1-86B2

Sequence location

3R:10,361,760..10,378,068 [-]

Recombination map

3-49

RefSeq locus

NT_033777 REGION:10361760..10378068

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (11 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables sequence-specific DNA binding

inferred from direct assay

(Durand et al., 2000)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables DNA binding

inferred from electronic annotation with InterPro:IPR003150

(InterPro Project Members, 2004-)

enables DNA-binding transcription factor activity, RNA polymerase II-specific

inferred from biological aspect of ancestor with PANTHER:PTN000289470

(GO Reference Genome Project, 2011-)

enables DNA-binding transcription factor activity

inferred from electronic annotation with InterPro:IPR039779

(InterPro Project Members, 2004-)

enables RNA polymerase II cis-regulatory region sequence-specific DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN000289470

(GO Reference Genome Project, 2011-)

Biological Process (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in nervous system development

inferred from mutant phenotype

(Dubruille et al., 2002)

involved_in sensory perception of sound

inferred from mutant phenotype

(Dubruille et al., 2002)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in dendrite morphogenesis

traceable author statement

(Gao and Bogert, 2003)

involved_in regulation of DNA-templated transcription

traceable author statement

(Gao and Bogert, 2003)

inferred from electronic annotation with InterPro:IPR003150

(InterPro Project Members, 2004-)

involved_in regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000289470

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000289470

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

UNCLASSIFIED DNA BINDING DOMAIN TRANSCRIPTION FACTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Rfx (Rfx) encodes a transcription factor involved in the differentiation of ciliated sensory neurons. [Date last reviewed: 2019-09-12]

Gene Group (FlyBase)

UNCLASSIFIED DNA BINDING DOMAIN TRANSCRIPTION FACTORS -: This group comprises DNA-binding transcription factors that do not classify under other domain-based transcription factor groups in FlyBase.

Summary (Interactive Fly)

transcription factor - RFX DNA-binding domain - essential regulator of ciliated sensory neuron differentiation - Forkhead transcription factor Fd3F cooperates with Rfx to regulate a gene expression program for mechanosensory cilia specialization.

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Rfx for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A0A0B4K653)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.48

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Rfx-RC

FBtr0305995

NM_001260092

4736

911

Rfx-RF

FBtr0337103

NM_001275535

4329

882

Rfx-RG

FBtr0337104

NM_001260093

3861

860

Rfx-RH

FBtr0337105

NM_001260094

4760

919

Rfx-RI

FBtr0337106

NM_001104268

4268

889

Rfx-RJ

FBtr0337107

NM_141742

4694

897

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Rfx-PC

FBpp0297137

99.8

911

4.73

FBpp0308017

96.8

882

6.94

FBpp0308018

94.2

860

6.93

FBpp0308019

100.7

919

4.76

FBpp0308020

97.1

889

4.69

FBpp0308021

98.0

897

4.72

Q9VGZ6

NP_649999

AAF54526

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rfx using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Rfx_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.49

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 10 -- 11

embryonic gnathal segment | restricted

Comment: a few cells

(Durand et al., 2000)

external sensory organ precursor cell of embryonic thoracic segment

Comment: 2 cells per hemisegment

(Durand et al., 2000)

external sensory organ precursor cell of embryonic abdominal segment

Comment: 2 cells per hemisegment

(Durand et al., 2000)

embryonic stage 11 -- 12

antennal primordium

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

dorsal sensory complex primordium

(Fisher et al., 2012)

embryonic optic lobe primordium

(Fisher et al., 2012)

labial sensory complex primordium

(Fisher et al., 2012)

labral sensory complex primordium

(Fisher et al., 2012)

maxillary sensory complex primordium

(Fisher et al., 2012)

ventral sensory complex anlage

(Fisher et al., 2012)

embryonic stage 11 -- 14

chordotonal organ precursor cell

(Cachero et al., 2011)

embryonic stage 12

external sensory organ precursor cell IIb

(Durand et al., 2000)

procephalic neurogenic region

(Durand et al., 2000)

embryonic stage 13 -- 16

dorsal sensory complex proper primordium

Comment: reported as dorsal/lateral sensory complexes

(Fisher et al., 2012)

embryonic brain

(Fisher et al., 2012)

embryonic head sensory system

(Fisher et al., 2012)

ventral sensory complex primordium

(Fisher et al., 2012)

embryonic optic lobe primordium

(Fisher et al., 2012)

embryonic stage 14

embryonic brain

(Durand et al., 2000)

external sensory organ precursor cell

(Cachero et al., 2011)

embryonic stage 14 -- 16

lch1 neuron

(Durand et al., 2000)

larval abdominal lch5 neuron

(Durand et al., 2000)

embryonic/larval head sense organ

(Durand et al., 2000)

embryonic stage 16

vch neuron

(Durand et al., 2000)

chordotonal neuron

(Cachero et al., 2011)

Additional Descriptive Data

Rfx

(Cachero et al., 2011)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11 -- 14

external sensory organ precursor cell

• nucleus

(Vandaele et al., 2001)

embryonic stage 12

procephalic neurogenic region | restricted

Comment: 2 small bilateral cell clusters

(Vandaele et al., 2001)

embryonic stage 14 -- 17

embryonic brain | restricted

(Vandaele et al., 2001)

embryonic stage 15

eo sensory organ accessory cell | faint

• nucleus

Comment: expression fades by stage 16

(Vandaele et al., 2001)

embryonic stage 15 -- 17

sensory neuron of external sensory organ

• nucleus

(Vandaele et al., 2001)

chordotonal neuron

• nucleus

(Vandaele et al., 2001)

larval stage

larval brain | restricted

(Vandaele et al., 2001)

P-stage

adult brain | restricted

(Vandaele et al., 2001)

external sensory organ precursor cell of leg disc

(Vandaele et al., 2001)

external sensory organ precursor cell of wing disc

(Vandaele et al., 2001)

external sensory organ precursor cell of antennal disc

(Vandaele et al., 2001)

chordotonal organ of leg | subset

Comment: femoral chordotonal organ

(Vandaele et al., 2001)

adult stage | male & spermatogenesis

elongation stage spermatid

(Vandaele et al., 2001)

Additional Descriptive Data

Rfx protein is initally expressed at embryonic stage 11, in external sensory organ precursor cells (SOPs) in the gnathal segments, and in the first two SOPs to appear in each thoracic and abdominal segment. At embryonic stages 12-14, Rfx is expressed in the progeny of these cells. At embryonic stage 15, Rfx is found predominantly the nuclei of chordotonal and external sensory organ neurons, and faintly in the accessory sister support cells of these neurons. Expression in the accessory cells fades during embryonic stage 16. Expression is maintained in the nuclei of chordotonal and external sensory organ neurons through the end of embryogenesis. Rfx is expressed in all external sensory organ neurons of the embryonic head, except for the Bolwig organ neuron. Rfx protein is localized to the nucleus. Rfx is also expressed in the embryonic brain, starting at embryonic stage 12, where is it expressed in two small bilateral cell clusters in the procephalic neurogenic region. Fron stage 14-17, this expression persists in a small number of cells abutting the optic lobe invagination. Similar expression is obsesrved in the larval and pupal brain lobes. Rfx is absent from larval imaginal disc SOPs; expression reappears later in development, in SOPs of pupal leg, wing and antennal discs. The first pupal expression is observed in the neurons of the femoral chordotonal organs. Rfx protein is also transiently expressed in elongation stage spermatids, but not in round spermatids nor in late condensing spermatids.

(Vandaele et al., 2001)

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Rfx in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Rfx

View exonic expression by tissue for Dmel\Rfx

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0020379

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41266/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0020379

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0020379

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0020379

Flygut - An atlas of the Drosophila adult midgut

FBgn0020379

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 17 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Rfx

Transgenic constructs containing regulatory region of Rfx

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene disrupted in

Df(3R)Exel6157

(Ryder, 2004.4.26)

Df(3R)hth

(Dubruille et al., 2002)

Df(3R)Rfx⁴⁹

(Dubruille et al., 2002)

Df(3R)S143702

(Dubruille et al., 2002)

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

Rfx^MI00053

lethal | male limited, with Scer\GAL4^Bx-MS1096

Rfx^{UAS.ORF.GW.Tag:HA}

lethal | male limited | cold sensitive, with Scer\GAL4^Bx-MS1096

Rfx^{UAS.ORF.GW.Tag:HA}

lethal - all die before end of larval stage | recessive

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Rfx^GD4148

viable

Rfx^c02503

viable, with Scer\GAL4^Mef2.PR

Rfx^GD4148

viable, with Scer\GAL4^pnr-MD237

Rfx^GD4148

viable, with Scer\GAL4^tin.CΔ4

Rfx^GD4148

Sterility

Allele

fertile

Rfx^c02503

Other Phenotypes

Allele

abnormal cell adhesion | oogenesis, with Scer\GAL4^slbo.2.6

Rfx^HMJ23335

abnormal neurophysiology (with Rfx⁴⁹)

Rfx²⁵³

abnormal neurophysiology (with Rfx²⁵³)

Rfx⁴⁹

abnormal smell perception | larval stage

Rfx⁴⁹

abnormal smell perception | larval stage (with Rfx⁴⁹)

Rfx²⁵³

abnormal smell perception | larval stage (with Rfx²⁵³)

Rfx⁴⁹

abnormal taste perception | larval stage (with Rfx⁴⁹)

Rfx²⁵³

abnormal taste perception | larval stage (with Rfx²⁵³)

Rfx⁴⁹

some die during larval stage | recessive

some die during pupal stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Rfx^GD4148

uncoordinated | adult stage (with Df(3R)S143702)

Rfx²⁵³

uncoordinated | adult stage (with Rfx⁴⁹)

Rfx²⁵³

uncoordinated | adult stage (with Rfx²⁵³)

Rfx⁴⁹

visible | female limited, with Scer\GAL4^Bx-MS1096

Rfx^{UAS.ORF.GW.Tag:HA}

visible | female limited | cold sensitive, with Scer\GAL4^Bx-MS1096

Rfx^{UAS.ORF.GW.Tag:HA}

Phenotype manifest in

Allele

abdominal lateral pentascolopidial chordotonal organ lch5

Rfx⁴⁹

abdominal lateral pentascolopidial chordotonal organ lch5 & dendrite

Rfx⁴⁹

abdominal lateral pentascolopidial chordotonal organ lch5 & scolopidial cilium

Rfx⁴⁹

antenna (with Rfx⁴⁹)

Rfx²⁵³

antenna (with Rfx²⁵³)

Rfx⁴⁹

border follicle cell, with Scer\GAL4^slbo.2.6

Rfx^HMJ23335

chordotonal organ & leg sensillum (with Df(3R)S143702)

Rfx²⁵³

chordotonal organ & leg sensillum & scolopidial cilium (with Df(3R)S143702)

Rfx²⁵³

Johnston's organ & scolopale (with Rfx⁴⁹)

Rfx²⁵³

Johnston's organ & scolopale (with Rfx²⁵³)

Rfx⁴⁹

Johnston's organ & scolopidial cilium (with Rfx⁴⁹)

Rfx²⁵³

Johnston's organ & scolopidial cilium (with Rfx²⁵³)

Rfx⁴⁹

Johnston's organ & scolopidium (with Rfx⁴⁹)

Rfx²⁵³

Johnston's organ & scolopidium (with Rfx²⁵³)

Rfx⁴⁹

macrochaeta (with Rfx⁴⁹)

Rfx²⁵³

macrochaeta (with Rfx²⁵³)

Rfx⁴⁹

sensillum campaniformium & wing sensillum (with Rfx⁴⁹)

Rfx²⁵³

sensillum campaniformium & wing sensillum (with Rfx²⁵³)

Rfx⁴⁹

sensillum campaniformium & wing sensillum & dendrite (with Rfx⁴⁹)

Rfx²⁵³

sensillum campaniformium & wing sensillum & dendrite (with Rfx²⁵³)

Rfx⁴⁹

wing | female limited, with Scer\GAL4^Bx-MS1096

Rfx^{UAS.ORF.GW.Tag:HA}

wing | female limited | cold sensitive, with Scer\GAL4^Bx-MS1096

Rfx^{UAS.ORF.GW.Tag:HA}

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (6)

Hsap\RFX2

12 of 14

Yes

Hsap\RFX3

12 of 14

Yes

Hsap\RFX1

11 of 14

Yes

Hsap\RFX4

4 of 14

Yes

Hsap\RFX6

3 of 14

Yes

Hsap\RFX8

2 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (6)

Rnor\Rfx2

12 of 14

Yes

Rnor\Rfx1

11 of 14

Yes

Rnor\Rfx3

11 of 14

Yes

Rnor\Rfx4

4 of 14

Yes

Rnor\Rfx6

3 of 14

Yes

Rnor\Rfx8

2 of 14

Yes

Mus musculus (laboratory mouse) (7)

Mmus\Rfx2

12 of 14

Yes

Mmus\Rfx3

12 of 14

Yes

Mmus\Rfx1

11 of 14

Yes

Mmus\Rfx4

4 of 14

Yes

Mmus\Rfx6

3 of 14

Yes

Mmus\Arid2

1 of 14

Mmus\Rfx8

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (7)

Xtro\rfx2

10 of 13

Yes

Xtro\rfx3

10 of 13

Yes

Xtro\rfx1

7 of 13

Yes

Xtro\rfx4

4 of 13

Yes

Xtro\LOC100494099

2 of 13

Yes

Xtro\rfx6

2 of 13

Yes

Xtro\trip13

1 of 13

Danio rerio (Zebrafish) (6)

Drer\rfx1b

12 of 14

Yes

Drer\rfx2

12 of 14

Yes

Drer\rfx3

12 of 14

Yes

Drer\rfx1a

11 of 14

Yes

Drer\rfx4

4 of 14

Yes

Drer\rfx6

3 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\daf-19

11 of 14

Yes

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP003943

11 of 12

Yes

Arabidopsis thaliana (thale-cress) (1)

Atha\RBE

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\RFX1

7 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\sak1

9 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Rfx. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

CG9727

3 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RFX2; regulatory factor X2

12 of 14

142765

RFX3; regulatory factor X3

12 of 14

601337

RFX1; regulatory factor X1

11 of 14

600006

RFX4; regulatory factor X4

4 of 14

603958

RFX6; regulatory factor X6

3 of 14

612659

MITCHELL-RILEY SYNDROME; MTCHRS

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

66413

DroID - A comprehensive database of gene and protein interactions.

FBgn0020379

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-49

Cytogenetic map

86B1-86B2

Sequence location

3R:10,361,760..10,378,068 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

86B1-86B2

Limits computationally determined from genome sequence between P{PZ}jumu⁰⁶⁴³⁹&P{lacW}l(3)j8B6^j8B6 and P{PZ}hth⁰⁵⁷⁴⁵

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

86A-86B

(determined by in situ hybridisation)

(Durand et al., 2000)

Experimentally Determined Recombination Data

Location

3-49

(FlyBase, 2016)

3-46.7

(Comeron et al., 2012)

(Durand and Couble, 1997)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (39)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

47933

w¹¹¹⁸; P{GMR44G12-GAL4}attP2

50222

w¹¹¹⁸; P{GMR45B08-GAL4}attP2

601440

w¹¹¹⁸; P{R44G12-p65.AD}JK22C

75767

w¹¹¹⁸; P{R45B08-p65.AD}attP40

68772

w¹¹¹⁸; P{R45B08-GAL4.DBD}attP2

600678

w¹¹¹⁸; P{R44G12-GAL4.DBD}attP2

601081

w¹¹¹⁸; P{VT039419-p65.AD}attP40

FlyORF

F004572

M{UAS-Rfx.ORF-CC}ZH-21F

F000709

M{UAS-Rfx.ORF.3xHA.GW}ZH-86Fb

F004881

M{UAS-Rfx.ORF-VNshort}ZH-86Fb

Korea

15200

P{VT039419-GAL4}attP2

VDRC

v10416

w¹¹¹⁸; P{GD4148}v10416

More stocks available...

Genomic Clones (26)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (5)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

MIP29292

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0020379

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0020379

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Vandaele et al., 2001)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

New stable cell line derived from S2-unspecified : New stable S2 cell lines were generated that contain genome edits (disruption in miRNA pairing sequences) for AGO1, hry, Kah, PolQ, wgn, zfh1, Rfx, CG11248, sam, mus81, wnd. A previously generated Dora edited line was also used.
(Kingston et al., 2022)

Other Comments

Rfx is an essential regulator of ciliated sensory neuron differentiation.

(Dubruille et al., 2002)

Rfx mutants show defects in chemosensory and mechanosensory behaviours. They have abnormal function and structure of neuronal cilia.

(Dubruille et al., 2002)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

CG6312

(Meadows, 2019.11.20, Balmer et al., 2015, Perkins et al., 2009)

RFX

(Laurençon et al., 2007, Gao and Bogert, 2003)

dRFX

(Hossain et al., 2023, Gogendeau and Basto, 2010, Dubruille et al., 2004, Otsuki et al., 2004, Durand et al., 2001, Laurencon et al., 2001, Vandaele et al., 2001, Durand et al., 2000, Durand et al., 2000, Vandaele et al., 1999, Durand et al., 1998)

dRfx

(Laurençon et al., 2007, Laurencon et al., 2003)

rfx

(Diggle et al., 2014, Soulavie et al., 2014, Brown et al., 2013, Enjolras et al., 2012, Han et al., 2003, Durand, 1999.2.16)

Name Synonyms

Regulatory factor X

(Cachero et al., 2011, Gao and Bogert, 2003)

Rfx

regulatory factor X

(Laurençon et al., 2007)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 54 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

41266

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A0A0B4K653

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A0A0B4K653

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0020379

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41266/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0020379

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0020379

FlyMine - An integrated database for Drosophila genomics

FBgn0020379

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG6312

MARRVEL_MODEL - MARRVEL (model organism gene)

41266

Linkouts

BioGRID - A database of protein and genetic interactions.

66413

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0020379

DroID - A comprehensive database of gene and protein interactions.

FBgn0020379

DRSC - Results frm RNAi screens

FBgn0020379

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Rfx_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0020379

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0020379

Flygut - An atlas of the Drosophila adult midgut

FBgn0020379

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC011734

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

References (86)

The following external sites may use different assemblies or annotations than FlyBase.

0.49

Report Sections