FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\ND-75
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General Information
Symbol
Dmel\ND-75
Species
D. melanogaster
Name
NADH dehydrogenase (ubiquinone) 75 kDa subunit
Annotation Symbol
CG2286
Feature Type
protein_coding_gene
FlyBase ID
FBgn0017566
Gene Model Status
Current
Stock Availability
12 publicly available
Enzyme Name (EC)
NADH:ubiquinone reductase (H(+)-translocating) (7.1.1.2)
Gene Summary
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity). (UniProt, Q94511)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

ND75, NDUFS1, NADH:ubiquinone reductase 75kD subunit precursor

Key Links
Genomic Location
Cytogenetic map
7E1-7E1
Sequence location
Recombination map
1-23
RefSeq locus
NC_004354 REGION:8248651..8251687
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (12 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
enables iron-sulfur cluster binding
(InterPro Project Members, 2004-)
inferred from electronic annotation with InterPro:IPR001041, InterPro:IPR010228, InterPro:IPR036010
(InterPro Project Members, 2004-)
contributes_to NADH dehydrogenase (ubiquinone) activity
inferred from sequence or structural similarity with UniProtKB:P28331
(Gene Ontology Curators, 2002-)
inferred from biological aspect of ancestor with PANTHER:PTN002267020
(GO Reference Genome Project, 2011-)
enables NADH dehydrogenase (ubiquinone) activity
inferred from electronic annotation with InterPro:IPR000283
(InterPro Project Members, 2004-)
enables oxidoreductase activity, acting on NAD(P)H
inferred from electronic annotation with InterPro:IPR010228, InterPro:IPR015405
(InterPro Project Members, 2004-)
enables oxidoreductase activity
inferred from electronic annotation with InterPro:IPR006656, InterPro:IPR006963, InterPro:IPR019574
(InterPro Project Members, 2004-)
Biological Process (5 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
involved_in ATP synthesis coupled electron transport
inferred from electronic annotation with InterPro:IPR000283
(InterPro Project Members, 2004-)
involved_in cellular respiration
inferred from sequence or structural similarity with UniProtKB:P28331
(Gene Ontology Curators, 2002-)
involved_in mitochondrial electron transport, NADH to ubiquinone
inferred by curator from GO:0045271
(Agip et al., 2023)
involved_in mitochondrial respiratory chain complex I assembly
inferred from biological aspect of ancestor with PANTHER:PTN002267020
(GO Reference Genome Project, 2011-)
involved_in reactive oxygen species metabolic process
inferred from sequence or structural similarity with UniProtKB:P28331
(Gene Ontology Curators, 2002-)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
part_of respiratory chain complex I
inferred from direct assay
(Agip et al., 2023)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
located_in membrane
inferred from electronic annotation with InterPro:IPR000283
(InterPro Project Members, 2004-)
is_active_in mitochondrial inner membrane
inferred by curator from GO:0045271
(Agip et al., 2023)
inferred from biological aspect of ancestor with PANTHER:PTN002267020
(GO Reference Genome Project, 2011-)
part_of respiratory chain complex I
inferred from sequence or structural similarity with UniProtKB:P28331
(Gene Ontology Curators, 2002-)
non-traceable author statement
(Rhooms et al., 2020, Porcelli et al., 2007)
inferred from biological aspect of ancestor with PANTHER:PTN002267020
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
Belongs to the complex I 75 kDa subunit family. (Q94511)
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
NADH dehydrogenase (ubiquinone) activity
a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4 H(+)(out) (7.1.1.2)
RHEA 29091:
Summaries
Gene Group (FlyBase)
MITOCHONDRIAL COMPLEX I - CORE SUBUNITS -
Mitochondrial Complex I is the first and largest holoenzyme of the mitochondrial electron transport chain. It oxidizes NADH from the tricarboxylic acid cycle and β oxidation of fatty acids, reduces ubiquinone and transports protons across the inner membrane, contributing to the proton-motive force. It is also involved in the cellular production of reactive oxygen species. Fourteen distinct subunits form the catalytic centers of the enzyme as they are directly involved in transferring electrons from NADH to ubiquinone, or for generation of the membrane potential - they are referred to as the 'core' or 'central' subunits and are conserved from the ancestral enzyme in bacteria to mammals. Seven core subunits are encoded by mtDNA, while the others are encoded the nuclear genome. (Adapted from PMID:23527692 and FBrf0244962.)
MITOCHONDRIAL COMPLEX I - TESTIS-SPECIFIC VARIANT -
Mitochondrial Complex I is the first and largest holoenzyme of the mitochondrial electron transport chain. It oxidizes NADH from the tricarboxylic acid cycle and β oxidation of fatty acids, reduces ubiquinone and transports protons across the inner membrane, contributing to the proton-motive force. It is also involved in the cellular production of reactive oxygen species. Fourteen distinct subunits form the catalytic centers of the enzyme as they are directly involved in transferring electrons from NADH to ubiquinone, or for generation of the membrane potential. (Adapted from PMID:23527692 and FBrf0244962.)
MITOCHONDRIAL COMPLEX I -
Mitochondrial Complex I is the first and largest holoenzyme of the mitochondrial electron transport chain. It oxidizes NADH from the tricarboxylic acid cycle and β oxidation of fatty acids, reduces ubiquinone and transports protons across the inner membrane, contributing to the proton-motive force. It is also involved in the cellular production of reactive oxygen species. Fourteen distinct subunits form the catalytic centers of the enzyme as they are directly involved in transferring electrons from NADH to ubiquinone, or for generation of the membrane potential. (Adapted from PMID:23527692 and FBrf0244962.)
Pathway (FlyBase)
OXIDATIVE PHOSPHORYLATION -
Oxidative phosphorylation (OXPHOS) is the coordinated transfer of electrons and protons leading to the production of ATP. The OXPHOS system is composed of five mitochondrial inner membrane complexes, Complexes I-IV comprise the electron respiratory chain and Complex V the ATP synthase. Complexes I and II oxidize the electron donors NADH and FADH2, respectively, derived from the tricarboxylic acid (TCA) cycle, and the electrons are transferred to the electron carrier ubiquinone which shuttles them to Complex III. Cytochrome c transfers electrons between Complex III and the terminal complex in the respiratory chain, Complex IV. Complex IV catalyzes electron transfer from reduced cytochrome c to oxygen. Complex I, II and IV couple electron transfer to proton transport across the inner mitochondrial membrane into the inner membrane space. This generates a proton electrochemical gradient which is used to drive the synthesis of ATP from ADP and inorganic phosphate by ATP synthase (Complex V). (Adapted from PMID:23356252 and PMID:11331900.)
Protein Function (UniProtKB)
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).
(UniProt, Q94511)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\ND-75 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q94511)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
PDB - An information portal to biological macromolecular structures
Comments on Gene Model

Gene model reviewed during 5.50

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
ND-75-RA
FBtr0071180
NM_078528
2571
731
ND-75-RB
FBtr0071181
NM_167152
2543
731
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
ND-75-PA
FBpp0071128
78.6
731
6.82
Q94511
NP_511083
AAF46356
ND-75-PB
FBpp0071129
78.6
731
6.82
Q94511
NP_727255
AAN09230
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

731 aa isoforms: ND-75-PA, ND-75-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Complex I is composed of about 45 different subunits.

(UniProt, Q94511)
Crossreferences
InterPro - A database of protein families, domains and functional sites
PDB - An information portal to biological macromolecular structures
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ND-75 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.13

Transcript Expression
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 5 of adulthood | male -- day 7 of adulthood | male
spermatozoon
(Wasbrough et al., 2010, Dorus et al., 2006)
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of respiratory chain complex I
inferred from direct assay
(Agip et al., 2023)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\ND-75 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 0 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of ND-75
Transgenic constructs containing regulatory region of ND-75
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation ( 0 )
Inferred from location ( 11 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal - all die before end of P-stage, with Scer\GAL4eya.enh1+E+2
partially lethal, with Scer\GAL4eya.enh1+E+2
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4tin.CΔ4
Other Phenotypes
Allele
abnormal locomotor behavior | adult stage, with Scer\GAL4elav.PU
abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4da.PU
abnormal neuroanatomy, with Scer\GAL4da.PU
abnormal neuroanatomy, with Scer\GAL4repo.PU
abnormal neuroanatomy | larval stage, with Scer\GAL4ey.PU
abnormal pain response | adult stage, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
increased cell death, with Scer\GAL4da.PU
increased cell number | third instar larval stage, with Scer\GAL4Antp-10
increased occurrence of cell division | larval stage, with Scer\GAL4ey.PU
neoplasia | larval stage, with Scer\GAL4ey.PU
short lived, with Scer\GAL4da.PU
short lived, with Scer\GAL4elav.PU
short lived, with Scer\GAL4repo.PU
visible | adult stage, with Scer\GAL4eya.enh1+E+2
Phenotype manifest in
Allele
adult brain, with Scer\GAL4da.PU
adult brain, with Scer\GAL4repo.PU
autophagosome | cell non-autonomous | somatic clone | third instar larval stage, with Scer\GAL4ap.PU
embryonic/larval dorsal tracheal branch | larval stage, with Scer\GAL4drm.7.1
eye, with Scer\GAL4eya.enh1+E+2
eye disc, with Scer\GAL4ey.PU
lamellocyte | larval stage, with Scer\GAL4Antp-10
lamellocyte | larval stage, with Scer\GAL4Ugt36A1.PK
lamellocyte | third instar larval stage, with Scer\GAL4Antp-10
mitochondrion, with Scer\GAL4da.PU
mitochondrion, with Scer\GAL4repo.PU
ommatidium, with Scer\GAL4ey.PU
wing disc dorsal compartment | cell non-autonomous | somatic clone | third instar larval stage, with Scer\GAL4ap.PU
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (1)
Hsap\NDUFS1
14 of 14
Yes
Yes
2  
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (1)
Rnor\Ndufs1
14 of 14
Yes
Yes
Mus musculus (laboratory mouse) (1)
Mmus\Ndufs1
13 of 14
Yes
Yes
Xenopus tropicalis (Western clawed frog) (2)
Xtro\ndufs1
8 of 13
Yes
Yes
Xtro\ndufa13
1 of 13
No
No
Danio rerio (Zebrafish) (1)
Drer\ndufs1
4 of 14
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\nuo-5
13 of 14
Yes
Yes
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP001653
11 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (1)
Atha\EMB1467
13 of 13
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\NAR1
1 of 13
Yes
No
Schizosaccharomyces pombe (Fission yeast) (0)
Escherichia coli (enterobacterium) (3)
Ecol\nuoG
9 of 11
Yes
Yes
Ecol\fdhF
1 of 11
No
Yes
Ecol\napA
1 of 11
No
Yes
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:ND-75. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (1)
CG17683
2 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 4 )
Potential Models Based on Orthology ( 1 )
Modifiers Based on Experimental Evidence ( 2 )
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
NDUFS1; NADH:ubiquinone oxidoreductase core subunit S1
14 of 14
157655
 
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
Interaction Browsers
View in FlyBase Interactions Browser   View in MIST tool (external link)
Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
ND-75 - Atg4a
experimental knowledge based
(Guruharsha et al., 2011)
ND-75 - CG11035
experimental knowledge based
(Guruharsha et al., 2011)
ND-75 - Flad1
experimental knowledge based
(Guruharsha et al., 2011)
ND-75 - Hmgs
experimental knowledge based
(Guruharsha et al., 2011)
ND-75 - JMJD6
experimental knowledge based
(Guruharsha et al., 2011)
ND-75 - Lst8
experimental knowledge based
(Guruharsha et al., 2011)
ND-75 - ND-13A
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-13B
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-15
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-19
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-20
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-23
cosedimentation, electron microscopy, molecular weight estimation by staining, experimental knowledge based
(Padavannil et al., 2023, Guruharsha et al., 2011)
ND-75 - ND-24
experimental knowledge based, cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023, Guruharsha et al., 2011)
ND-75 - ND-30
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-39
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-42
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-49
experimental knowledge based
(Guruharsha et al., 2011)
ND-75 - ND-51
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-ACP
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-AGGG
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-ASHI
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-B12
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-B14
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-B14.5A
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-B14.5B
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-B14.7
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-B15
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-B16.6
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-B17
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-B17.2
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-B18
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-B22
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-MNLL
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-MWFE
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - ND-PDSW
cosedimentation, electron microscopy, molecular weight estimation by staining, experimental knowledge based
(Padavannil et al., 2023, Guruharsha et al., 2011)
ND-75 - ND-SGDH
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - NP15.6
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - NdufA3
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - NdufV3
experimental knowledge based
(Guruharsha et al., 2011)
ND-75 - SPE
experimental knowledge based
(Guruharsha et al., 2011)
ND-75 - Tg
experimental knowledge based
(Guruharsha et al., 2011)
ND-75 - hiro
experimental knowledge based
(Guruharsha et al., 2011)
ND-75 - igl
experimental knowledge based
(Guruharsha et al., 2011)
ND-75 - mt:ND1
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - mt:ND2
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - mt:ND3
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - mt:ND4L
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - mt:ND5
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - mt:ND6
cosedimentation, electron microscopy, molecular weight estimation by staining
(Padavannil et al., 2023)
ND-75 - nwk
experimental knowledge based
(Guruharsha et al., 2011)
RNA-protein
Physical Interaction
Assay
References
ND-75 - smg
anti bait coimmunoprecipitation, primer specific pcr
(Chartier et al., 2015)
Summary of Genetic Interactions
Interaction Browsers
View in FlyBase Interactions Browser   View in MIST tool (external link)
Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
ND-75
suppressible
Akt
(Sinenko et al., 2011)
ND-75
suppressible
foxo
(Sinenko et al., 2011)
ND-75
suppressible
Sod2
(Sinenko et al., 2011)
ND-75
suppressible
spi
(Sinenko et al., 2011)
ND-75
enhanceable
Wwox
(Choo et al., 2015)
ND-75
suppressible
Wwox
(Choo et al., 2015)
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
N
suppressible
ND-75
(Lee et al., 2013)
trc
enhanceable
ND-75
(Hudson et al., 2023)
External Data
Subunit Structure (UniProtKB)
Complex I is composed of about 45 different subunits.
(UniProt, Q94511 )
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
FlyBase
OXIDATIVE PHOSPHORYLATION
External Links
FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
External Data
Linkouts
KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Class of Gene
Genomic Location and Detailed Mapping Data
Chromosome (arm)
X
Recombination map
1-23
Cytogenetic map
7E1-7E1
Sequence location
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
7E1-7E1
Limits computationally determined from genome sequence between P{EP}sdtEP1143 and P{EP}CG1632EP1583
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
7E1-7E2
(Caggese, 1996.10.24)
7D20-7D22
(determined by in situ hybridisation)
(Caggese et al., 1999)
Experimentally Determined Recombination Data
Location

1-23

(FlyBase, 2016)

1-22.6

(Comeron et al., 2012)

1-

(Caggese, 1996.10.24)
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (12)
Genomic Clones (12)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (151)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
Antibody Information
Laboratory Generated Antibodies
 
Commercially Available Antibodies
 
Cell Line Information
Publicly Available Cell Lines
 
    Other Stable Cell Lines
     
      Other Comments
      Relationship to Other Genes
      Source for database merge of
      Additional comments
      Nomenclature History
      Source for database identify of

      Source for identity of: ND75 CG2286

      (Zhang, 1999.11)

      Source for identity of: ND-75 ND75

      (Porcelli and Marygold, 2014.9.16)
      Nomenclature comments

      Renamed from 'ND75' to 'ND-75' to match the format of other genes encoding NADH dehydrogenase (ubiquinone) subunits.

      (Porcelli and Marygold, 2014.9.16)
      Etymology
      Synonyms and Secondary IDs (16)
      Reported As
      Symbol Synonym
      CG2286
      (Curcio et al., 2024, Müller-Eigner et al., 2024, Rhooms et al., 2020, Kolkhof et al., 2017, Çiçek et al., 2016, Choo et al., 2015, Foriel et al., 2015, Fukuoh et al., 2014, Jaspers et al., 2012, Cammarato et al., 2011, Müller et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Puri et al., 2008, Porcelli et al., 2007, Caggese et al., 2006, Gong et al., 2004, McGraw et al., 2004, Kang et al., 2002, Fulton et al., 2001)
      ND-75
      (Mukherjee et al., 2025, Tsakiri et al., 2025, Curcio et al., 2024, Müller-Eigner et al., 2024, Salminen et al., 2024, Vesala et al., 2024, Everman et al., 2023, Granat et al., 2023, Hudson et al., 2023, Lam Wong and Verheyen, 2021, Sinenko et al., 2021, Perez-Gomez et al., 2020, Scialò et al., 2020, Bouska et al., 2019, Elkholi et al., 2019, Fowler et al., 2019, Yee et al., 2019, Staats et al., 2018, Transgenic RNAi Project members, 2017-, Porcelli and Marygold, 2014.9.16)
      ND75
      (Sobrido-Cameán et al., 2025, Byrns et al., 2024, Fan et al., 2024, Shamapari and Nagaraj, 2024, Ren et al., 2023, Bolus et al., 2020, Miller et al., 2020, Xia et al., 2020, Toshniwal et al., 2019, van den Ameele and Brand, 2019, Tan et al., 2018, Hermle et al., 2017, Katheder et al., 2017, Lindström et al., 2017, Tan et al., 2017, Ma et al., 2016, Chartier et al., 2015, Choo et al., 2015, Hegde et al., 2014, Rogers and Rogina, 2014, Guitart et al., 2013, Handler et al., 2013, Lee et al., 2013, Lim et al., 2013, O'Sullivan et al., 2013, Owusu-Ansah et al., 2013, Wu et al., 2013, Yang et al., 2013, Gurudatta et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Friedman et al., 2011, Hochmuth et al., 2011, Sinenko et al., 2011, Sinenko et al., 2011, Avet-Rochex et al., 2010, Müller et al., 2010, Sinenko et al., 2010, Wasbrough et al., 2010, Owusu-Ansah and Banerjee, 2009, Akashi et al., 2006, Caggese et al., 2006, Dorus et al., 2006, Ko et al., 2006, Tripoli et al., 2005)
      NDUFS1
      (Agip et al., 2023, Sinenko et al., 2021, van den Ameele and Brand, 2019, Owusu-Ansah et al., 2013)
      NDUS1
      (Liu et al., 2018)
      NUAM
      (Caggese et al., 2006)
      Ndufs1
      (Meyer et al., 2024)
      dNDUFS1
      (Rhooms et al., 2020)
      Name Synonyms
      FBpp0071128
      (Yin et al., 2013)
      NADH dehydrogenase (ubiquinone) 75 kDa subunit
      (Porcelli and Marygold, 2014.9.16)
      NADH ubiquinone oxidoreductase 75 kDa
      (Porcelli et al., 2007)
      NADH-ubiquinone oxidoreductase 75 kDa subunit
      (Caggese et al., 2006)
      NADH:ubiquinone oxidoreductase 75 kDa subunit
      (Tripoli et al., 2005)
      NADH:ubiquinone reductase 75 kDa subunit
      (Caggese, 1996.10.24)
      NADH:ubiquinone reductase 75-kD subunit precursor
      (Kang et al., 2002)
      NADH:ubiquinone reductase 75kD subunit precursor
      (Jaspers et al., 2012, Cammarato et al., 2011)
      Secondary FlyBase IDs
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        Study result (0)
        Result
        Result Type
        Title
        External Crossreferences and Linkouts ( 61 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
        EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
        FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
        FlyMine - An integrated database for Drosophila genomics
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        MARRVEL_MODEL - MARRVEL (model organism gene)
        PDB - An information portal to biological macromolecular structures
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.
        References (141)