FlyBase Gene Report: Dmel\mei-P22

General Information

Symbol

Dmel\mei-P22

Species

D. melanogaster

Name

meiotic P22

Annotation Symbol

CG14827

Feature Type

protein_coding_gene

FlyBase ID

FBgn0016036

Gene Model Status

Current

Stock Availability

3 publicly available

Gene Summary

meiotic P22 (mei-P22) encodes a protein thought to form a dimer with the TopoVIA subunit encoded by mei-W68 and is required for meiotic double strand breakes in female meiosis. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Key Links

Genomic Location

Cytogenetic map

65E9-65E9

Sequence location

3L:7,244,388..7,245,383 [+]

Recombination map

3-19

RefSeq locus

NT_037436 REGION:7244388..7245383

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (2 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in reciprocal meiotic recombination

inferred from mutant phenotype

(Joyce and McKim, 2009, Liu et al., 2002, McKim et al., 1998)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in reciprocal meiotic recombination

traceable author statement

(McKim et al., 2000)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in chromatin

inferred from direct assay

(Lake et al., 2011)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Protein Family (UniProt)

Belongs to the TOP6B-like family. (Q9VS36)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Protein Function (UniProtKB)

Required for formation of the mei-W68-mediated double-strand breaks (DSBs) that initiate meiotic recombination.

(UniProt, Q9VS36)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\mei-P22 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VS36)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

mei-P22-RA

FBtr0076836

NM_079232

996

331

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

mei-P22-PA

FBpp0076547

37.4

331

10.10

Q9VS36

NP_523956

AAF50593

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mei-P22 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

mei-P22_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.50

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in chromatin

inferred from direct assay

(Lake et al., 2011)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\mei-P22 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\mei-P22

View exonic expression by tissue for Dmel\mei-P22

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38797/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0016036

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0016036

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0016036

Flygut - An atlas of the Drosophila adult midgut

FBgn0016036

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 8 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of mei-P22

Transgenic constructs containing regulatory region of mei-P22

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3L)Exel6111

(Ryder, 2004.4.26)

Inferred from location ( 1 )

Dp(3;2)GV-CH321-22F24

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal - majority die | adult stage, with Scer\GAL4^pnr-MD237

mei-P22^GD8456

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

mei-P22^GD8456

viable, with Scer\GAL4^Mef2.PR

mei-P22^GD8456

Other Phenotypes

Allele

abnormal meiotic cell cycle

abnormal meiotic cell cycle | female

mei-P22¹⁰³

Phenotype manifest in

Allele

karyosome | oogenesis

mei-P22^unspecified

oocyte

mei-P22^unspecified

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (0)

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:mei-P22. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

mei-P22 - 14-3-3ζ

two hybrid

(Hu et al., 2017.6.13)

mei-P22 - Awh

two hybrid

(Hu et al., 2017.6.13)

mei-P22 - CG14074

two hybrid

(Hu et al., 2017.6.13)

mei-P22 - CG17186

two hybrid

(Hu et al., 2017.6.13)

mei-P22 - lwr

two hybrid

(Hu et al., 2017.6.13)

mei-P22 - vers

two hybrid

(Hu et al., 2017.6.13)

mei-P22 - vilya

two hybrid

(Lake et al., 2015)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

mei-P22

suppressible

Spc105R

(Joshi et al., 2024)

mei-P22

suppressible

Spc105R|Ndc80

(Joshi et al., 2024)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Brca2

suppressible

mei-P22

(Klovstad et al., 2008)

Gcna

suppressible

mei-P22

(Bhargava et al., 2020)

mei-41

suppressible

mei-P22

(McKim et al., 2000)

mio

suppressible

mei-P22

(Iida and Lilly, 2004)

okr

suppressible

mei-P22

(Ghabrial and Schupbach, 1999)

spn-B

suppressible

mei-P22

(Ferguson et al., 2012, Liu et al., 2002)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

64240

DroID - A comprehensive database of gene and protein interactions.

FBgn0016036

MIST (genetic) - An integrated Molecular Interaction Database

38797

MIST (protein-protein) - An integrated Molecular Interaction Database

38797

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-19

Cytogenetic map

65E9-65E9

Sequence location

3L:7,244,388..7,245,383 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

65E9-65E9

Limits computationally determined from genome sequence between P{PZ}corn⁰⁴²⁰² and P{lacW}Cdc27^L7123

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

65E10-65F2

(McKim, 1999.10.27)

65E-65E

(Sekelsky et al., 1999)

65E-65E

(determined by in situ hybridisation)

(McKim, 1998.3.10)

Experimentally Determined Recombination Data

Location

3-19

(FlyBase, 2016)

3-16.9

(Comeron et al., 2012)

3-21.5

(McKim, 1998.3.10)

Left of (cM)

(McKim, 1998.3.10)

Right of (cM)

(McKim, 1998.3.10)

Notes

Stocks and Reagents

Stocks (3)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

4931

y¹ w¹/Dp(1;Y)y⁺; P{lacW}mei-P22^P22; sv^spa-pol

FlyORF

F002304

M{UAS-mei-P22.ORF.3xHA.GW}ZH-86Fb

VDRC

v18399

w¹¹¹⁸; P{GD8456}v18399/TM3

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (1)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

RT02925

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0016036

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0016036

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Identification: In a genetic screen for meiotic mutants.

(Sekelsky et al., 1999)

mei-P22 is required for the initiation of meiotic recombination.

(Sekelsky et al., 1999)

Mutant females show no meiotic recombination. Achiasmate segregation systems still function, though inefficiently.

(Sekelsky et al., 1999)

Intragenic recombination at ry is used as an assay of the effects of mutation on meiotic crossing over.

(McKim et al., 1998)

Required for the initiation of meiotic recombination.

(McKim et al., 1996)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG14827

(Mount, 2001.8.14, Liu, 2000.9.22)

MEI-P22

(Robert et al., 2016, Tanneti et al., 2008, Mehrotra et al., 2004, McKim, 1999.10.27)

Mei-P22

(Lake et al., 2015, Nurminsky, 2007)

Name Synonyms

meiotic P22

(John et al., 2016, McKim et al., 1996)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 30 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38797

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VS36

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VS36

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q3YNE0

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VS36

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38797/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0016036

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0016036

FlyMine - An integrated database for Drosophila genomics

FBgn0016036

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG14827

MARRVEL_MODEL - MARRVEL (model organism gene)

38797

Linkouts

BioGRID - A database of protein and genetic interactions.

64240

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0016036

DroID - A comprehensive database of gene and protein interactions.

FBgn0016036

DRSC - Results frm RNAi screens

FBgn0016036

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

mei-P22_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0016036

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0016036

Flygut - An atlas of the Drosophila adult midgut

FBgn0016036

MIST (genetic) - An integrated Molecular Interaction Database

38797

MIST (protein-protein) - An integrated Molecular Interaction Database

38797

References (75)

The following external sites may use different assemblies or annotations than FlyBase.

-0.50

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