FlyBase Gene Report: Dmel\Calx

General Information

Symbol

Dmel\Calx

Species

D. melanogaster

Name

Na/Ca-exchange protein

Annotation Symbol

CG5685

Feature Type

protein_coding_gene

FlyBase ID

FBgn0013995

Gene Model Status

Current

Stock Availability

37 publicly available

Gene Summary

Na/Ca-exchange protein (Calx) encodes a Na-Ca exchanger involved in phototransduction and response to endoplasmic reticulum stress. [Date last reviewed: 2019-09-12] (FlyBase Gene Snapshot)

All Summaries

Also Known As

NCX, Dmel/Ncx

Key Links

Genomic Location

Cytogenetic map

93A4-93B3

Sequence location

3R:20,978,183..21,015,613 [+]

Recombination map

3-70

RefSeq locus

NT_033777 REGION:20978183..21015613

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (15 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables calcium ion binding

inferred from direct assay

(Wu et al., 2011)

enables calcium:sodium antiporter activity

inferred from mutant phenotype

(Wang et al., 2005)

inferred from direct assay

(Schwarz and Benzer, 1997)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables calcium:sodium antiporter activity

traceable author statement

(Webel et al., 2002)

inferred from biological aspect of ancestor with PANTHER:PTN000933900

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR004836

(InterPro Project Members, 2004-)

Biological Process (9 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in neuromuscular process controlling posture

inferred from mutant phenotype

(Akitake et al., 2015)

involved_in phototransduction

inferred from mutant phenotype

(Wang et al., 2005)

involved_in response to endoplasmic reticulum stress

inferred from high throughput mutant phenotype

(Chow et al., 2013)

Terms Based on Predictions or Assertions (6 terms)

CV Term

Evidence

References

involved_in calcium ion export across plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000933900

(GO Reference Genome Project, 2011-)

involved_in calcium ion transport

inferred from electronic annotation with InterPro:IPR004836

(InterPro Project Members, 2004-)

involved_in cell communication

inferred from electronic annotation with InterPro:IPR003644

(InterPro Project Members, 2004-)

involved_in intracellular calcium ion homeostasis

inferred from biological aspect of ancestor with PANTHER:PTN000933900

(GO Reference Genome Project, 2011-)

involved_in sodium ion transmembrane transport

inferred from biological aspect of ancestor with PANTHER:PTN000933900

(GO Reference Genome Project, 2011-)

involved_in transmembrane transport

inferred from electronic annotation with InterPro:IPR004837

(InterPro Project Members, 2004-)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

is_active_in axon

inferred from biological aspect of ancestor with PANTHER:PTN000933900

(GO Reference Genome Project, 2011-)

located_in membrane

inferred from electronic annotation with InterPro:IPR003644, InterPro:IPR004836, InterPro:IPR004837

(InterPro Project Members, 2004-)

is_active_in postsynapse

inferred from biological aspect of ancestor with PANTHER:PTN000933900

(GO Reference Genome Project, 2011-)

is_active_in sarcolemma

inferred from biological aspect of ancestor with PANTHER:PTN000933900

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

SLC8 FAMILY OF SODIUM/CALCIUM EXCHANGERS

Protein Family (UniProt)

Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19) family. SLC8 subfamily. (Q9VDG5)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

calcium:sodium antiporter activity
RHEA 29255:

Summaries

Gene Snapshot

Na/Ca-exchange protein (Calx) encodes a Na-Ca exchanger involved in phototransduction and response to endoplasmic reticulum stress. [Date last reviewed: 2019-09-12]

Gene Group (FlyBase)

SLC8 FAMILY OF SODIUM/CALCIUM EXCHANGERS -: Solute carrier family 8 (SLC8) members facilitate Ca²⁺ flux across the plasma membrane by utilizing a counter-Na⁺ electrochemical gradient. (Adapted from FBrf0216387).

Protein Function (UniProtKB)

Na(+)/Ca(2+) antiporter that couples the energy of a Na(+) electrochemical gradient to the movement of Ca(2+) against an electrochemical gradient across a membrane, which contributes to the regulation of cytoplasmic Ca(2+) levels (PubMed:8817385, PubMed:9252464). Mediates Na(+)/Ca(2+) exchange in photoreceptor cells and involved in controlling Ca(2+) levels during phototransduction, affecting magnitude of the photoresponse, activation kinetics, signal amplification, response termination, and light adaptation (PubMed:15694324). Light induced depolarization of photoreceptor cells, resulting in Na(+) and Ca(2+) entry through trp/transient receptor potential protein channels, is essential for photoreceptor cell function but may result in toxic levels of cytoplasmic Ca(2+) (PubMed:15694324). Na(+)/Ca(2+) antiporter regulation of Ca(2+) levels protects photoreceptor cells from light-dependent retinal degeneration (PubMed:15694324).

(UniProt, Q9VDG5)

Summary (Interactive Fly)

involved in phototransduction and response to endoplasmic reticulum stress

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Calx for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VDG5)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

Comments on Gene Model

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model reviewed during 5.40

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.53

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Calx-RA

FBtr0083998

NM_079699

6137

950

Calx-RB

FBtr0083999

NM_169940

3786

950

Calx-RC

FBtr0084000

NM_169941

4728

950

Calx-RD

FBtr0309202

NM_001260283

3734

950

Calx-RE

FBtr0309203

NM_001260284

3589

963

Calx-RF

FBtr0309204

NM_001260285

4127

960

Calx-RG

FBtr0309205

NM_001260286

6705

973

Calx-RH

FBtr0344316

NM_001300505

5947

955

Calx-RI

FBtr0344317

NM_001300506

7361

657

Additional Transcript Data and Comments

Reported size (kB)

9.0, 8.2, 7.0, 5.6, 4.9, 4.0, >=15 (northern blot); 5.8 (longest cDNA)

(Schwarz and Benzer, 1997)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Calx-PA

FBpp0083402

106.1

950

4.55

FBpp0083403

106.1

950

4.55

FBpp0083404

106.1

950

4.55

FBpp0301141

106.2

950

4.60

FBpp0301142

107.8

963

4.54

FBpp0301143

107.3

960

4.60

FBpp0301144

109.0

973

4.59

FBpp0310708

106.8

955

4.59

FBpp0310709

74.4

657

4.60

A0A0B4LHD9

NP_001287435

AHN57434

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

950 aa isoforms: Calx-PA, Calx-PB, Calx-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

950 (aa)

(Schwarz and Benzer, 1997)

Comments

Calx cRNA microinjected into Xenopus oocytes induces calcium uptake.

(Schwarz and Benzer, 1997)

External Data

Domain

Contains 2 Calx-beta domains (PubMed:19361442, PubMed:19815561, PubMed:22000518). Calx-beta domain 1 binds two pairs of Ca(2+) ions (PubMed:19361442, PubMed:19815561). Binding of Ca(2+) induces a conformational shift that inhibits the Na(+)/Ca(2+) antiporter activity (PubMed:19815561). Calx-beta domain 2 does not bind Ca(2+) (PubMed:19361442). Calx-beta domain 2 contributes to the cooperative binding of Ca(2+) to Calx-beta domain 1 (PubMed:22000518).

(UniProt, Q9VDG5)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Calx using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

2.11

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous | uniform

(Haug-Collet et al., 1999)

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

anlage in statu nascendi

(Fisher et al., 2012)

central brain anlage in statu nascendi

(Fisher et al., 2012)

dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal ectoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

cell | subset of ventral midline of embryo

(Haug-Collet et al., 1999)

embryonic stage 16

neuron | subset of larval ventral nerve cord

(Haug-Collet et al., 1999)

adult stage

photoreceptor neuron of eye disc posterior to the morphogenetic furrow

(Haug-Collet et al., 1999)

adult brain

(Haug-Collet et al., 1999)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Schwarz and Benzer, 1997)

adult stage

adult head

(Schwarz and Benzer, 1997)

adult brain

(Schwarz and Benzer, 1997)

optic nerve

(Schwarz and Benzer, 1997)

retina

(Schwarz and Benzer, 1997)

Additional Descriptive Data

Calx transcrip is ubiquitously expressed in pre-blastoderm embryos, but is undetectable in blastoderm embryos. Expression reappears at stage 11; by stage 12, Calx transcript is expressed in two cells per segment, one on each side of the ventral midline.

(Haug-Collet et al., 1999)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

mechanosensory neuron of femoral chordotonal organ

• cell body

(Akitake et al., 2015)

femoral chordotonal organ scolopale cell

(Akitake et al., 2015)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Calx in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Calx

View exonic expression by tissue for Dmel\Calx

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0013995

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42481/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0013995

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0013995

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0013995

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5685

Flygut - An atlas of the Drosophila adult midgut

FBgn0013995

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Calx

Stages(s) 4-6
Stages(s) 7-8

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 18 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Calx

Transgenic constructs containing regulatory region of Calx

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene disrupted in

Df(3R)Exel6272

(Ryder, 2004.4.26)

Gene not disrupted in

Df(3R)e-N19

(Wang et al., 2005)

Df(3R)e-R1

(Wang et al., 2005)

Df(3R)H-B79

(Wang et al., 2005)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Calx^GD3094

viable, with Scer\GAL4^Mef2.PR

Calx^GD3094

viable, with Scer\GAL4^pnr-MD237

Calx^GD3094

viable, with Scer\GAL4^tin.CΔ4

Calx^GD3094

Other Phenotypes

Allele

abnormal neuroanatomy

Calx^B

abnormal neurophysiology

abnormal neurophysiology, with Calx^A

Calx^ninaE.PW

abnormal neurophysiology, with Calx^ninaE.PW

Calx^A

short lived

short lived (with Calx^A)

Calx^B

short lived (with Calx^B)

Calx^A

uncoordinated | adult stage

Calx^B

Phenotype manifest in

Allele

eye, with Calx^A

Calx^ninaE.PW

eye, with Calx^ninaE.PW

Calx^A

eye photoreceptor cell

Calx^A

rhabdomere

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (13)

Hsap\SLC8A3

13 of 14

Yes

Hsap\SLC8A2

12 of 14

Yes

Hsap\SLC8A1

10 of 14

Yes

Hsap\ADGRV1

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (12)

Rnor\Slc8a3

13 of 14

Yes

Rnor\Slc8a2

12 of 14

Yes

Rnor\Slc8a1

11 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (13)

Mmus\Slc8a3

13 of 14

Yes

Mmus\Slc8a2

11 of 14

Yes

Mmus\Slc8a1

10 of 14

Yes

Mmus\Adgrv1

2 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (12)

Xtro\slc8a3

11 of 13

Yes

Xtro\slc8a2

10 of 13

Yes

Xtro\LOC100487899

9 of 13

Yes

Xtro\slc8a1

6 of 13

Yes

Xtro\cyp2a6.1

1 of 13

Xtro\htr6

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (19)

Drer\slc8a2a

12 of 14

Yes

Drer\slc8a3

12 of 14

Yes

Drer\slc8a4a

12 of 14

Yes

Drer\slc8a4b

12 of 14

Yes

Drer\slc8a2b

11 of 14

Yes

Drer\slc8a1a

10 of 14

Yes

Drer\slc8a1b

10 of 14

Yes

Drer\adgrv1

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (10)

Cele\ncx-2

12 of 14

Yes

Cele\ncx-1

8 of 14

Yes

Cele\ncx-3

7 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (9)

Agam\AgaP_AGAP002859

12 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (6)

Atha\MHX

11 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\YDL206W

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPAC3A12.06c

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Calx. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (10)

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

zyd

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

Calx^ninaE.PW

exacerbates combined saposin deficiency

modeled by Sap-r^e01294

(Hindle et al., 2017)

Calx^UAS.cMa

ameliorates neurodegenerative disease

modeled by trp^P365

(Huang et al., 2016)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SLC8A3; solute carrier family 8 member A3

13 of 14

607991

SLC8A2; solute carrier family 8 member A2

12 of 14

601901

SLC8A1; solute carrier family 8 member A1

10 of 14

182305

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

RNA-RNA

Physical Interaction

Assay

References

Calx - mir-286

luminiscence technology

(Yu et al., 2025)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Calx

suppressible

trp

(Wang et al., 2005)

Calx

suppressible

Trpγ

(Akitake et al., 2015)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Cpn

suppressible

Calx

(Weiss et al., 2012)

Sap-r

enhanceable

Calx

(Hindle et al., 2017)

Sk2

suppressible

Calx

(Yonamine et al., 2011)

trp

suppressible

Calx

(Huang et al., 2016, Wang et al., 2005, Wang et al., 2005)

Trpγ

suppressible

Calx

(Akitake et al., 2015)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

67452

DroID - A comprehensive database of gene and protein interactions.

FBgn0013995

MIST (genetic) - An integrated Molecular Interaction Database

42481

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene_with_edited_transcript

(Rodriguez et al., 2012, Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-70

Cytogenetic map

93A4-93B3

Sequence location

3R:20,978,183..21,015,613 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

93A4-93B3

Limits computationally determined from genome sequence between P{PZ}Atpα⁰¹¹⁶⁴ and P{PZ}mod(mdg4)⁰⁷⁰³⁸&P{lacW}mod(mdg4)^L3101

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

93B1-93B2

(Schwarz, 1997.6.18, Schwarz and Benzer, 1997)

93A-93B

(determined by in situ hybridisation)

(Ruknudin et al., 1997, Hyde et al., 1990)

93B1-93B2

(determined by in situ hybridisation)

(Schwarz, 1996)

Determined by Nancy Bonini.

(Schwarz, 1996)

Experimentally Determined Recombination Data

Location

3-70

(FlyBase, 2016)

3-73.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (37)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

24496

w^*; Calx^A

24497

w^*; Calx^B

47357

w¹¹¹⁸; P{GMR43A12-GAL4}attP2

48155

w¹¹¹⁸; P{GMR44C07-GAL4}attP2

50209

w¹¹¹⁸; P{GMR44D10-GAL4}attP2

50240

w¹¹¹⁸; P{GMR45F12-GAL4}attP2

50244

w¹¹¹⁸; P{GMR45G06-GAL4}attP2

50276

w¹¹¹⁸; P{GMR46F10-GAL4}attP2

5685R-2

v104789

v42660

w¹¹¹⁸; P{GD3094}v42660

v42661

w¹¹¹⁸; P{GD3094}v42661

More stocks available...

Genomic Clones (22)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (73)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0013995

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0013995

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

The Na⁺/Ca²⁺ exchange activity of the Calx protein is necessary to prevent retinal degeneration due to Ca²⁺ overload.

(Wang et al., 2005)

Functional characterisation of Calx.

(Ruknudin et al., 1997)

When expressed in Xenopus oocytes and characterized electrophysiologically using the giant excised patch technique, Calx exhibits properties similar to previously characterized Na⁺-Ca²⁺ exchangers including intracellular Na+ affinities, current-voltage relationships, and sensitivity to the peptide inhibitor, XIP. However, Calx shows a completely opposite response to cytoplasmic Ca²⁺: Calx is inhibited by cytoplasmic Ca²⁺.

(Hryshko et al., 1996)

Heterologous expression of Calx in Xenopus oocytes shows that it encodes a bona fide sodium-calcium exchanger; unlike mammalian retinal exchangers, it does not depend on potassium for activity. Calx encodes two novel protein motifs, Calx-^alpha and Calx-^beta. Both are intragenically duplicated, but they probably have different functions: Calx-^alpha is likely to encode residues central to calcium export, while Calx-^beta may mediate intracellular signalling or cytoskeletal anchoring.

(Schwarz, 1996)

Calx cDNA, when expressed and assayed in Xenopus oocytes, generates calcium transport with both the specificity and efficacy of its mammalian homologs.

(Schwarz and Benzer, 1993)

Identification: cDNA clone that is expressed exclusively or predominantly in the adult visual system.

(Hyde et al., 1990)

Identification: Head-not-embryo cDNA clone.

(Palazzolo et al., 1989)

RNA is expressed in the head but cannot be detected in the early embryo.

(Palazzolo et al., 1989)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Calx CG5685

(Gong, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

9C5

(Hyde et al., 1990)

CALX

(Gutorov et al., 2022, de Souza Degenhardt et al., 2021, Cardoso et al., 2020, Lucke et al., 2020, Halty-deLeon et al., 2018, Abiko et al., 2016, Giladi et al., 2012, Abell et al., 2011, Wu et al., 2011, Wu et al., 2010, Wu et al., 2009)

CALX1.1

(Dyck et al., 1998)

CG5685

(Metwally et al., 2019, Balakrishnan et al., 2015, Cammarato et al., 2011, Keleman et al., 2009.8.5, Perkins et al., 2009, Brody et al., 2002)

CalX

(Liu et al., 2020, Voolstra and Huber, 2020, Hindle et al., 2017, Akitake et al., 2015, Jaiswal et al., 2015, Rosenbaum et al., 2014, Hilge, 2012, Weiss et al., 2012, Richter et al., 2011, Elsaesser et al., 2010, Wang et al., 2008, Rosenbaum et al., 2006, Wang et al., 2005)

Dmel/Ncx

(Ruknudin et al., 1997, Ruknudin et al., 1997)

NCX

(Rodríguez et al., 2021, Vajente et al., 2020, Dyck et al., 1997, Valdivia, 1997.3.19)

Ncx

(Ruknudin et al., 1997)

anon-93ABa

calX

(Palladino and Miller, 2007, Wang et al., 2005)

calx

(Kon et al., 2021, Liu et al., 2020, Cao et al., 2018, Asteriti et al., 2017, Huang et al., 2016, Akitake et al., 2015, Chen et al., 2015, Yonamine et al., 2011, Elsaesser et al., 2010, Satoh et al., 2010, Qian and Bodmer, 2009, Wang et al., 2008, Wang et al., 2005, Wang et al., 2005)

Name Synonyms

Na/Ca-exchange protein

(Cammarato et al., 2011, Qian and Bodmer, 2009)

Secondary FlyBase IDs

FBgn0020315

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 88 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

42481

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VDG5

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VDG5

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VDG5

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0013995

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42481/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0013995

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0013995

FlyMine - An integrated database for Drosophila genomics

FBgn0013995

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG5685

MARRVEL_MODEL - MARRVEL (model organism gene)

42481

PDB - An information portal to biological macromolecular structures

Linkouts

BioGRID - A database of protein and genetic interactions.

67452

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0013995

DroID - A comprehensive database of gene and protein interactions.

FBgn0013995

DRSC - Results frm RNAi screens

FBgn0013995

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0013995

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0013995

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5685

Flygut - An atlas of the Drosophila adult midgut

FBgn0013995

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC009330

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

42481

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (136)

The following external sites may use different assemblies or annotations than FlyBase.

2.11

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