FlyBase Gene Report: Dmel\Mgtor

General Information

Symbol

Dmel\Mgtor

Species

D. melanogaster

Name

Megator

Annotation Symbol

CG8274

Feature Type

protein_coding_gene

FlyBase ID

FBgn0013756

Gene Model Status

Current

Stock Availability

11 publicly available

Gene Summary

Megator (Mgtor) encodes a nuclear pore protein that is found in the nuclear matrix surrounding the chromosomes at interphase. During mitosis Mgtor product dynamically reorganizes, together with other nuclear and cytoplasmic proteins (such as those encoded by Skel, Chro, east, and Asator), to form a gel-like spindle matrix embedding the microtubule spindle apparatus. Mgtor product is important for normal cell cycle progression, functioning as a spatial regulator of cell cycle factors. [Date last reviewed: 2018-09-13] (FlyBase Gene Snapshot)

All Summaries

Also Known As

Mtor, Bx34, Tpr, l(2)k03905

Key Links

Genomic Location

Cytogenetic map

48C5-48C5

Sequence location

2R:11,852,617..11,861,202 [+]

Recombination map

2-65

RefSeq locus

NT_033778 REGION:11852617..11861202

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (49 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables chromatin DNA binding

inferred from direct assay

(Vaquerizas et al., 2010)

enables histone acetyltransferase binding

inferred from physical interaction with UniProtKB:O02193

(Aleman et al., 2021)

enables protein binding

inferred from physical interaction with UniProtKB:Q86BS3

(Qi et al., 2004)

inferred from physical interaction with UniProtKB:Q9VRQ2

(Lince-Faria et al., 2009)

inferred from physical interaction with UniProtKB:Q9VCH5

(Pascual-Garcia et al., 2017)

inferred from physical interaction with UniProtKB:Q7KND8

(Cunha-Silva et al., 2020)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables ribonucleoprotein complex binding

inferred from sequence or structural similarity with SGD:S000001803

(Gene Ontology Curators, 2002-)

enables structural constituent of nuclear pore

inferred from biological aspect of ancestor with PANTHER:PTN001052033

(GO Reference Genome Project, 2011-)

Biological Process (26 terms)

Terms Based on Experimental Evidence (24 terms)

CV Term

Evidence

References

involved_in chromatin organization

inferred from mutant phenotype

(Vaquerizas et al., 2010)

involved_in chromatin remodeling

inferred from mutant phenotype

(Vaquerizas et al., 2010)

involved_in dosage compensation by hyperactivation of X chromosome

inferred from mutant phenotype

(Aleman et al., 2021, Mendjan et al., 2006)

involved_in germ-line stem-cell niche homeostasis

inferred from mutant phenotype

(Liu et al., 2015)

involved_in male germ-line stem cell asymmetric division

inferred from mutant phenotype

(Liu et al., 2015)

involved_in mitotic spindle assembly checkpoint signaling

inferred from mutant phenotype

(Lince-Faria et al., 2009)

involved_in mitotic spindle elongation

inferred from mutant phenotype

(Lince-Faria et al., 2009)

involved_in negative regulation of transcription by RNA polymerase II

inferred from mutant phenotype

(Aleman et al., 2021)

involved_in positive regulation of cell division

inferred from mutant phenotype

(Qi et al., 2004)

involved_in positive regulation of intracellular protein transport

inferred from mutant phenotype

(Lince-Faria et al., 2009)

involved_in positive regulation of mitotic cell cycle spindle assembly checkpoint

inferred from mutant phenotype

(Lince-Faria et al., 2009)

involved_in positive regulation of mitotic nuclear division

inferred from mutant phenotype

(Qi et al., 2004)

involved_in positive regulation of transcription by RNA polymerase II

inferred from mutant phenotype

(Vaquerizas et al., 2010)

involved_in regulation of DNA-templated transcription

inferred from mutant phenotype

(Vaquerizas et al., 2010)

involved_in regulation of metaphase plate congression

inferred from mutant phenotype

(Lince-Faria et al., 2009)

involved_in regulation of mitotic cell cycle

inferred from mutant phenotype

(Lince-Faria et al., 2009)

involved_in regulation of mitotic sister chromatid separation

inferred from mutant phenotype

(Lince-Faria et al., 2009)

involved_in regulation of mitotic spindle assembly

inferred from mutant phenotype

(Lince-Faria et al., 2009)

involved_in regulation of mitotic spindle organization

inferred from mutant phenotype

(Qi et al., 2004)

involved_in response to endoplasmic reticulum stress

inferred from high throughput mutant phenotype

(Chow et al., 2013)

involved_in response to heat

inferred from direct assay

(Zimowska and Paddy, 2002)

involved_in spindle assembly

inferred from direct assay

(Qi et al., 2004)

NOT involved_in regulation of chromosome segregation

inferred from mutant phenotype

(Lince-Faria et al., 2009)

NOT involved_in regulation of mitotic spindle elongation

inferred from mutant phenotype

(Lince-Faria et al., 2009)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in mRNA export from nucleus

inferred from biological aspect of ancestor with PANTHER:PTN001052033

(GO Reference Genome Project, 2011-)

involved_in protein import into nucleus

inferred from electronic annotation with InterPro:IPR012929

(InterPro Project Members, 2004-)

involved_in regulation of mitotic spindle assembly

inferred from biological aspect of ancestor with PANTHER:PTN000431177

(GO Reference Genome Project, 2011-)

Cellular Component (18 terms)

Terms Based on Experimental Evidence (18 terms)

CV Term

Evidence

References

located_in euchromatin

inferred from direct assay

(Vaquerizas et al., 2010)

located_in metaphase plate

inferred from direct assay

(Qi et al., 2004)

located_in midbody

inferred from direct assay

(Qi et al., 2004)

colocalizes_with mitotic spindle

inferred from direct assay

(Katsani et al., 2008)

located_in mitotic spindle

inferred from direct assay

(Qi et al., 2004)

located_in nuclear envelope

inferred from direct assay

(Lince-Faria et al., 2009, Zimowska et al., 1997)

inferred from direct assay

(Lye et al., 2014, Katsani et al., 2008)

inferred from high throughput direct assay

(Lye et al., 2014)

located_in nuclear inclusion body

inferred from direct assay

(Qi et al., 2004, Zimowska et al., 1997)

located_in nuclear lamina

inferred from direct assay

(Qi et al., 2004)

located_in nuclear matrix

inferred from direct assay

(Zimowska et al., 1997)

located_in nuclear membrane

inferred from direct assay

(Yao et al., 2012)

located_in nuclear periphery

inferred from direct assay

(Vaquerizas et al., 2010, Zimowska and Paddy, 2002)

part_of nuclear pore

inferred from direct assay

(Cunha-Silva et al., 2020, Yao et al., 2012, Zimowska and Paddy, 2002)

inferred from direct assay

(Zimowska et al., 1997)

located_in nucleolus

inferred from direct assay

(Zimowska and Paddy, 2002)

located_in nucleoplasm

inferred from high throughput direct assay

(Lye et al., 2014)

located_in nucleus

inferred from direct assay

(Yao et al., 2012, Lince-Faria et al., 2009, Qi et al., 2004, Zimowska and Paddy, 2002)

inferred from direct assay

(Vaquerizas et al., 2010)

located_in spindle matrix

inferred from direct assay

(Lince-Faria et al., 2009)

located_in spindle midzone

inferred from direct assay

(Qi et al., 2004)

located_in spindle

inferred from direct assay

(Yao et al., 2012)

NOT located_in kinetochore

inferred from direct assay

(Lince-Faria et al., 2009)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of nuclear pore

inferred from biological aspect of ancestor with PANTHER:PTN001052033

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

NUCLEAR PORE COMPLEX

Protein Family (UniProt)

Belongs to the TPR family. (A1Z8P9)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

NUCLEAR PORE COMPLEX -: The Nuclear Pore Complex (NPC) is a large protein complex embedded in the nuclear membrane that facilitates nucleocytoplasmic traffic. The NPC is composed of approximately 30 different proteins: the nucleoporins. The NPC displays 8-fold symmetry and each nucleoporin is present in multiple copies. From the central ring that forms the pore, filaments protrude into the cytoplasm (cytoplasmic filaments) and the nucleoplasm (nuclear basket). Many nucleoporins contain FG repeats which are thought to act as a diffusion barrier and docking sight for transport receptors (karyopherins). Export and import is controlled by the interaction of the karyopherins with localization and export sequences. (Adapted from FBrf0220062 and PMID:21495847).

Protein Function (UniProtKB)

Component of the nuclear pore complex (NPC), a complex required for the trafficking across the nuclear envelope (PubMed:9152019). Functions as a scaffolding element in the nuclear phase of the NPC (PubMed:12027452, PubMed:15356261). Plays a role in chromosomal organization and gene expression regulation; stimulates transcription by promoting the formation of an open chromatin environment (PubMed:12027452, PubMed:20174442). Binds chromatin to nucleoporin-associated regions (NARs) that define transcriptionally active regions of the genome (PubMed:20174442). Associates with extended chromosomal regions that alternate between domains of high density binding with those of low occupancy (PubMed:20174442). Preferentially binds to NARs of the male X chromosome (PubMed:20174442). In males, together with Nup153, required for the localization of the male-specific lethal (MSL) histone acetyltransferase complex to the X chromosome and therefore for the transcription of dosage compensation genes (PubMed:16543150). In males, restrains dosage-compensated expression at the level of nascent transcription probably by interacting with the MSL complex and by modulating RNA Polymerase II phosphorylation status and activity (PubMed:34133927). During mitosis forms a gel-like spindle matrix complex together with Skeletor (Skel), Chro, east, and Asator embedding the microtubule spindle apparatus (PubMed:15356261, PubMed:15962301, PubMed:19273613, PubMed:22855526). During interphase localizes Mad1 to the nuclear pore complex and thereby might act as a scaffold to assemble the Mad1-C-Mad2 complex, a heterotetramer that catalyzes the structural conversion of open-Mad2 (O-Mad2) into closed-Mad2 (C-Mad2) which is essential for spindle-assembly checkpoint (SAC) (PubMed:31913420). During the metaphase-anaphase transition and before chromosome congression, is phosphorylated by Msp-1; this modification releases Mad1 from the nuclear pore complex and thereby promotes assembly of SAC ensuring a timely and effective recruitment of spindle checkpoint proteins like Mad1, Mad2 and Mps1 to unattached kinetochores (KT) (PubMed:22855526, PubMed:26714316, PubMed:31913420). In testes, has a role in stem cell asymmetric division and maintenance via regulation of mitotic spindle assembly checkpoint (SAC) complex (PubMed:26714316).

(UniProt, A1Z8P9)

Summary (Interactive Fly)

signaling protein that promotes the recruitment of Mad2 and Mps1 to unattached kinetochores, mediating normal mitotic duration and spindle assembly checkpoint response

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Mgtor for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A1Z8P9)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Mgtor-RA

FBtr0088033

NM_057719

7431

2346

Mgtor-RB

FBtr0332464

NM_001273974

7780

2346

Additional Transcript Data and Comments

Reported size (kB)

8 (northern blot)

(Zimowska et al., 1997)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Mgtor-PA

FBpp0087140

262.4

2346

4.75

FBpp0304737

262.4

2346

4.75

A1Z8P9

NP_001260903

AGB93436

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

2346 aa isoforms: Mgtor-PA, Mgtor-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

2346 (aa); 260 (kD observed); 262 (kD predicted)

(Zimowska et al., 1997)

Comments

External Data

Subunit Structure (UniProtKB)

Part of the nuclear pore complex (NPC) (PubMed:12027452, PubMed:22855526, PubMed:9152019). Associates with male-specific lethal (MSL) histone acetyltransferase complex (PubMed:16543150). Interacts with Mad2; the interaction is required for efficient recruitment of Mad2 to unattached kinetochore and occurs in a microtubule-independent manner (PubMed:19273613). Interacts with Mad1 (N-terminus) (PubMed:31913420). Interacts with Chro, east and Asator; the interaction is part of a macromolecular complex forming the spindle matrix during mitosis (PubMed:15356261, PubMed:15962301, PubMed:19890914). Interacts with Nup98 (PubMed:28366641). In males, interacts with histone acetyltransferase mof (PubMed:34133927).

(UniProt, A1Z8P9)

Post Translational Modification

Mps1-mediated phosphorylation disrupts interaction with Mad1 during mitosis.

(UniProt, A1Z8P9)

Domain

The N-terminal coiled-coil domain is required for both nuclear pore complex (NPC) and spindle matrix localization.

The C-terminal domain is required for interchromosomal localization during interphase (PubMed:15356261). The C-terminal domain is sufficient for localization to the nuclear lamina as well as for spindle localization (PubMed:15356261).

(UniProt, A1Z8P9)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mgtor using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Mtor_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.57

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage & larval stage & adult stage

(Zimowska et al., 1997)

adult stage | male & spermatogenesis

cyst progenitor cell

(Chen et al., 2013)

male germline stem cell

(Liu et al., 2015, Chen et al., 2013)

spermatogenesis & adult stage | male

somatic cell of testis

(Liu et al., 2015)

male germline cell

(Liu et al., 2015)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood | male -- day 7 of adulthood | male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Mgtor protein localizes to the nuclear interior in embryonic, larval and adult cell types studied.

(Zimowska et al., 1997)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in euchromatin

inferred from direct assay

(Vaquerizas et al., 2010)

located_in metaphase plate

inferred from direct assay

(Qi et al., 2004)

located_in midbody

inferred from direct assay

(Qi et al., 2004)

colocalizes_with mitotic spindle

inferred from direct assay

(Katsani et al., 2008)

located_in mitotic spindle

inferred from direct assay

(Qi et al., 2004)

located_in nuclear envelope

inferred from direct assay

(Lince-Faria et al., 2009, Zimowska et al., 1997)

inferred from direct assay

(Lye et al., 2014, Katsani et al., 2008)

inferred from high throughput direct assay

(Lye et al., 2014)

located_in nuclear inclusion body

inferred from direct assay

(Qi et al., 2004, Zimowska et al., 1997)

located_in nuclear lamina

inferred from direct assay

(Qi et al., 2004)

located_in nuclear matrix

inferred from direct assay

(Zimowska et al., 1997)

located_in nuclear membrane

inferred from direct assay

(Yao et al., 2012)

located_in nuclear periphery

inferred from direct assay

(Vaquerizas et al., 2010, Zimowska and Paddy, 2002)

part_of nuclear pore

inferred from direct assay

(Cunha-Silva et al., 2020, Yao et al., 2012, Zimowska and Paddy, 2002)

inferred from direct assay

(Zimowska et al., 1997)

located_in nucleolus

inferred from direct assay

(Zimowska and Paddy, 2002)

located_in nucleoplasm

inferred from high throughput direct assay

(Lye et al., 2014)

located_in nucleus

inferred from direct assay

(Yao et al., 2012, Lince-Faria et al., 2009, Qi et al., 2004, Zimowska and Paddy, 2002)

inferred from direct assay

(Vaquerizas et al., 2010)

located_in spindle matrix

inferred from direct assay

(Lince-Faria et al., 2009)

located_in spindle midzone

inferred from direct assay

(Qi et al., 2004)

located_in spindle

inferred from direct assay

(Yao et al., 2012)

NOT located_in kinetochore

inferred from direct assay

(Lince-Faria et al., 2009)

Expression Deduced from Reporters

Reporter: PBac{602.P.SVS-1}Mgtor^CPTI001044

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult optic lobe

(Knowles-Barley, 2011.8.24)

adult brain cell body rind

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Mgtor in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Mgtor

View exonic expression by tissue for Dmel\Mgtor

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36264/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0013756

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0013756

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0013756

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8274

Flygut - An atlas of the Drosophila adult midgut

FBgn0013756

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Mgtor

Transgenic constructs containing regulatory region of Mgtor

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2R)ths238

(Kadam et al., 2009)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Mef2.PR

Mgtor^GD13871

lethal | recessive

Mgtor^k03905

lethal - all die during embryonic stage

Mgtor^k03905

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Mgtor^GD13871

viable

Mgtor^rv1

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Mgtor^GD13871

viable, with Scer\GAL4^tin.CΔ4

Mgtor^GD13871

Other Phenotypes

Allele

abnormal body color, with Scer\GAL4^pnr-MD237

Mgtor^GD13871

abnormal mitotic cell cycle, with Scer\GAL4^{VP16.nanos.UTR}

decreased cell number | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

decreased cell number | adult stage | clone of cells

Mgtor^k03905

decreased cell number | adult stage | germline clone

Mgtor^k03905

decreased cell number | adult stage | somatic clone

Mgtor^k03905

decreased occurrence of cell division | somatic clone

Mgtor^k03905

increased cell death | adult stage | clone of cells

Mgtor^k03905

increased cell number | adult stage, with Scer\GAL4^C587

Mgtor^HMS00735

some die during embryonic stage

Mgtor^k03905

some die during pupal stage, with Scer\GAL4^pnr-MD237

Mgtor^GD13871

visible, with Scer\GAL4^pnr-MD237

Mgtor^GD13871

Phenotype manifest in

Allele

centrosome | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

cyst progenitor cell, with Scer\GAL4^NP1624

Mgtor^HMS00735

cyst progenitor cell | adult stage, with Scer\GAL4^C587

Mgtor^HMS00735

cyst progenitor cell | adult stage | somatic clone

Mgtor^k03905

germinal proliferation center | adult stage, with Scer\GAL4^C587

Mgtor^HMS00735

germinal proliferation center | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

germline stem cell, with Scer\GAL4^NP1624

Mgtor^HMS00735

male germline cell, with Scer\GAL4^NP1624

Mgtor^HMS00735

male germline stem cell | adult stage, with Scer\GAL4^C587

Mgtor^HMS00735

male germline stem cell | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

male germline stem cell | adult stage | germline clone

Mgtor^k03905

mesonotum, with Scer\GAL4^pnr-MD237

Mgtor^GD13871

mitotic spindle | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

neuroblast | somatic clone | third instar larval stage

Mgtor^k03905

testis | adult stage, with Scer\GAL4^C587

Mgtor^HMS00735

testis | adult stage, with Scer\GAL4^{VP16.nanos.UTR}

testis | adult stage | clone of cells

Mgtor^k03905

testis | adult stage | germline clone

Mgtor^k03905

trichogen cell, with Scer\GAL4^pnr-MD237

Mgtor^GD13871

wing, with Scer\GAL4^Bx-MS1096

Mgtor^HMS00735

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (5)

Hsap\TPR

13 of 14

Yes

Hsap\LEKR1

1 of 14

Yes

Hsap\TXLNA

1 of 14

Hsap\TXLNB

1 of 14

Hsap\TXLNG

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (5)

Rnor\Tpr

13 of 14

Yes

Rnor\Lekr1

1 of 14

Yes

Rnor\Txlna

1 of 14

Rnor\Txlnb

1 of 14

Rnor\Txlng

1 of 14

Mus musculus (laboratory mouse) (5)

Mmus\Tpr

12 of 14

Yes

Mmus\Lekr1

1 of 14

Yes

Mmus\Txlna

1 of 14

Mmus\Txlnb

1 of 14

Mmus\Txlng

1 of 14

Xenopus tropicalis (Western clawed frog) (1)

Xtro\tpr

6 of 13

Yes

Danio rerio (Zebrafish) (5)

Drer\tprb

13 of 14

Yes

Drer\tpra

11 of 14

Yes

Drer\txlna

1 of 14

Drer\txlnba

1 of 14

Drer\txlnbb

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\npp-21

3 of 14

Yes

Cele\T22C1.6

1 of 14

Anopheles gambiae (African malaria mosquito) (2)

Agam\AgaP_AGAP001754

11 of 12

Yes

Agam\AgaP_AGAP009667

1 of 12

Arabidopsis thaliana (thale-cress) (1)

Atha\NUA

10 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\MLP1

8 of 13

Yes

Scer\MLP2

7 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\nup211

8 of 12

Yes

Spom\alm1

2 of 12

Yes

Spom\spo15

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Mgtor. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (2)

CG5886

1 of 13

dpy

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Mgtor^KK102690

exacerbates C9orf72 frontotemporal dementia and/or amyotrophic lateral sclerosis

modeled by Zzzz\poly-PR^{25.UAS.Tag:FLAG}

(Boeynaems et al., 2016)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TPR; translocated promoter region, nuclear basket protein

13 of 14

189940

INTELLECTUAL DEVELOPMENTAL DISORDER, AUTOSOMAL RECESSIVE 79; MRT79

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Mgtor - Asator

pull down, anti tag western blot, two hybrid

(Qi et al., 2009)

Mgtor - Chro

anti bait coimmunoprecipitation, western blot

(Qi et al., 2004)

Mgtor - EcR

anti bait coimmunoprecipitation, western blot

(Pascual-Garcia et al., 2017)

Mgtor - Hrb87F

anti bait coimmunoprecipitation, western blot

(Singh and Lakhotia, 2015)

Mgtor - Lkb1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Mgtor - MBD-R2

anti bait coimmunoprecipitation, western blot

(Raja et al., 2010, Mendjan et al., 2006)

Mgtor - Mad1

pull down, western blot, anti tag coimmunoprecipitation, Identification by mass spectrometry, anti bait coimmunoprecipitation

(Cunha-Silva et al., 2020, Raich et al., 2018)

Mgtor - Nup98-96

anti bait coimmunoprecipitation, western blot

(Pascual-Garcia et al., 2017)

Mgtor - Psc

anti bait coimmunoprecipitation, western blot

(Lagarou et al., 2008)

Mgtor - Sce

anti bait coimmunoprecipitation, peptide massfingerprinting, western blot

(Lagarou et al., 2008)

Mgtor - east

anti bait coimmunoprecipitation, western blot

(Qi et al., 2005)

Mgtor - mad2

anti bait coimmunoprecipitation, western blot

(Lince-Faria et al., 2009)

Mgtor - mof

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti bait coimmunoprecipitation, western blot

(Aleman et al., 2021, Mendjan et al., 2006)

Mgtor - msl-3

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Mendjan et al., 2006)

RNA-protein

Physical Interaction

Assay

References

Mgtor - lncRNA:Hsrω

anti bait coimmunoprecipitation, quantitative reverse transcription pcr

(Singh and Lakhotia, 2015)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Liu et al., 2015)

suppressible

(Liu et al., 2015)

suppressible

(Liu et al., 2015)

suppressible

(Li et al., 2017)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Myt1

suppressible

Mgtor

(Willms et al., 2020)

External Data

Subunit Structure (UniProtKB)

(UniProt, A1Z8P9 )

Linkouts

BioGRID - A database of protein and genetic interactions.

62050

DroID - A comprehensive database of gene and protein interactions.

FBgn0013756

MIST (protein-protein) - An integrated Molecular Interaction Database

36264

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Nucleocytoplasmic transport

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-65

Cytogenetic map

48C5-48C5

Sequence location

2R:11,852,617..11,861,202 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

48C5-48C5

Limits computationally determined from genome sequence between P{EP}wal^EP825&P{PZ}wal⁰²⁵¹⁶ and P{PZ}Ef1α48D⁰¹²⁷⁵

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

48C3-48C3

(Zimowska, 1997.3.4)

48C5-48C6

(determined by in situ hybridisation)

(Spradling et al., 1999)

48C-48C

(determined by in situ hybridisation)

(Zimowska et al., 1997)

48C-48C

(Zimowska et al., 1997)

48C5-48C6

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-65

(FlyBase, 2016)

2-66.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

93153

y¹ w^*; P{Mgtor.mCherry}2

10537

y¹ w^67c23; P{lacW}Mgtor^k03905/CyO

80645

y¹ w^*; TI{CRIMIC.TG4.1}Mgtor^{CR00760-TG4.1}

32941

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00735}attP2

Kyoto

111163

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}Mgtor^k03905 /CyO y⁺

NIG-Fly

8274R-2

P{NIG.8274R}2/SM6a, Duox^Cy

HMJ21187

y¹ v¹; P{TRiP.HMJ21187}attP40/CyO

HMS00735

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00735}attP2

2RG-0437

y² VhaAC39-1^cho-1 v¹; P{U6-Mgtor.NIG.sgRNA}attP40/CyO

VDRC

v110218

P{KK102690}VIE-260B

v24265

w¹¹¹⁸; P{GD13871}v24265

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (33)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0013756

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0013756

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Lagarou et al., 2008, Zimowska et al., 1997)

Commercially Available Antibodies

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

12F10-5F11

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene and injected into S2 cells causes a reduction in the number of cells undergoing mitosis.

(Qi et al., 2004)

Mtor localises to the nuclear pore complex and interchromosomal regions during interphase, but reorganises to a spindle matrix structure colocalizing with Skeletor during prophase.

(Rath et al., 2003)

Homozygous embryos die before hatching and have spindle abnormalities and chromosome segregation defects.

(Rath et al., 2003)

Area matching Drosophila Tpr homologue gene, Acc. No. U91980.

(Davies, 2001.3.15)

Isolated from a 0-16 hour embryo cDNA expression library, using monoclonal antibodies Mtor and Bx93 as probes.

(Zimowska et al., 1997)

Mtor has been cloned and sequenced, and its expression pattern and localisation within the cell has been determined.

(Zimowska et al., 1997)

Mtor gene product localises to the nuclear periphery in interphase, remains concentrated in the chromosomal region during metaphase and early anaphase, and isolates biochemically in the nuclear pore complex-lamina fraction (FBrf0048434).

(Zimowska-Handler et al., 1995)

Relationship to Other Genes

Source for database merge of

Source for merge of: Mtor l(2)k03905

(Qi et al., 2004)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Tpr Megator

(Johansen, 2003.2.18)

Source for identity of: Mgtor Mtor

(FlyBase, 2009-)

Nomenclature comments

Changed gene symbol 'Mtor' to 'Mgtor' to minimise confusion with the 'Tor' gene, which is the ortholog of vertebrate MTOR and is often referred to as such in flies.

(FlyBase, 2009-)

Etymology

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

Bx34

(Qi et al., 2004, Stathopoulos et al., 2004, Giot et al., 2003, Zimowska et al., 1997, Zimowska et al., 1996, Zimowska-Handler et al., 1995, Zimowska-Handler et al., 1995, Zimowska-Handler et al., 1994)

CG8274

(National Institute of Genetics Fly Stocks, 2023-, National Institute of Genetics Fly Stocks, 2022-, Hosono et al., 2015, Chen et al., 2012, Müller et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Mason and Goldfarb, 2009, Sabri et al., 2007)

MTOR

(Raices and D'Angelo, 2012, Prestel et al., 2010)

Megator

(Stenberg and Larsson, 2011, Qi et al., 2005)

Mgtor

(Sachweh et al., 2025)

Mtor

(McQuarrie et al., 2023, Aleman et al., 2021, Liang et al., 2021, Makki and Meller, 2021, Willms et al., 2020, Levis, 2018.8.30, Raich et al., 2018, Li et al., 2017, Pascual-Garcia et al., 2017, Boeynaems et al., 2016, Brewer-Jensen et al., 2016, Kuleesha et al., 2016, Puah and Wasser, 2016, Liu et al., 2015, Gonzalez et al., 2014, Lye et al., 2014, Chen et al., 2013, Chow et al., 2013, Kwon et al., 2013, Hessle et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Mihailovich et al., 2012, Yao et al., 2012, Kharchenko et al., 2011, Senger et al., 2011, Kallappagoudar et al., 2010, Müller et al., 2010, Raja et al., 2010, Vaquerizas et al., 2010, Wasbrough et al., 2010, Grimaud and Becker, 2009, Hessle et al., 2009, Kadam et al., 2009, Lince-Faria et al., 2009, Mason and Goldfarb, 2009, Patalano et al., 2009, Doheny et al., 2008, Katsani et al., 2008, Lagarou et al., 2008, Somma et al., 2008, Straub and Becker, 2007, Mendjan et al., 2006)

TPR

(Neumann et al., 2010, Raja et al., 2010, Sabri et al., 2007, Lasky, 2000)

Tor

(Chang et al., 2024)

Tpr

(Senger et al., 2011, Jolly and Lakhotia, 2006, Zimowska and Paddy, 2002, Paddy, 2000, Zimowska and Paddy, 1999, Zimowska and Paddy, 1999, Lamian et al., 1998, Zimowska, 1997.3.4)

l(2)k03905

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

mtor

(Hampoelz et al., 2019)

Name Synonyms

Megator

(Cunha-Silva et al., 2020, Yao et al., 2018, Lakhotia, 2017, Puah and Wasser, 2016, Raices and D'Angelo, 2012, Singh and Lakhotia, 2012, Yao et al., 2012, Vaquerizas et al., 2010, Johansen and Johansen, 2009, Lince-Faria et al., 2009, Mason and Goldfarb, 2009, Qi et al., 2009, Katsani et al., 2008, Lagarou et al., 2008, Somma et al., 2008, Johansen, 2003.2.18, Rath et al., 2003)

lethal (2) k03905

Secondary FlyBase IDs

FBgn0019919
FBgn0022212

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 61 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36264

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A1Z8P9

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

A1Z8P9

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A1Z8P9

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36264/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0013756

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0013756

FlyMine - An integrated database for Drosophila genomics

FBgn0013756

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG8274

MARRVEL_MODEL - MARRVEL (model organism gene)

36264

Linkouts

BioGRID - A database of protein and genetic interactions.

62050

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0013756

DroID - A comprehensive database of gene and protein interactions.

FBgn0013756

DRSC - Results frm RNAi screens

FBgn0013756

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

12F10-5F11

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Mtor_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0013756

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0013756

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8274

Flygut - An atlas of the Drosophila adult midgut

FBgn0013756

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0013756

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC002131

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Nucleocytoplasmic transport

MIST (protein-protein) - An integrated Molecular Interaction Database

36264

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (137)

The following external sites may use different assemblies or annotations than FlyBase.

-0.57

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