FlyBase Gene Report: Dmel\Gem3

General Information

Symbol

Dmel\Gem3

Species

D. melanogaster

Name

Gemin 3

Annotation Symbol

CG6539

Feature Type

protein_coding_gene

FlyBase ID

FBgn0011802

Gene Model Status

Current

Stock Availability

12 publicly available

Enzyme Name (EC)

RNA helicase (3.6.4.13)

Gene Summary

Gemin 3 (Gem3) encodes an RNA helicase and a member of the SMN-Gemins complex. It is a component of the cytoplasmic U snRNP body and nuclear gems. It is required in the motor system for viability, neuromuscular junction development and motor behaviour. [Date last reviewed: 2019-09-26] (FlyBase Gene Snapshot)

All Summaries

Also Known As

l(3)rL562, Dhh1, dGem3

Key Links

Genomic Location

Cytogenetic map

67E3-67E3

Sequence location

3L:10,459,081..10,463,101 [-]

Recombination map

3-33

RefSeq locus

NT_037436 REGION:10459081..10463101

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (15 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with UniProtKB:Q9VV74

(Cauchi et al., 2008)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR011545

(InterPro Project Members, 2004-)

enables mRNA binding

inferred from biological aspect of ancestor with PANTHER:PTN002776867

(GO Reference Genome Project, 2011-)

enables nucleic acid binding

inferred from electronic annotation with InterPro:IPR011545

(InterPro Project Members, 2004-)

enables RNA helicase activity

inferred from biological aspect of ancestor with PANTHER:PTN002774595

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR014014

(InterPro Project Members, 2004-)

Biological Process (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in adult locomotory behavior

inferred from mutant phenotype

(Borg and Cauchi, 2013)

involved_in flight behavior

inferred from mutant phenotype

(Borg and Cauchi, 2013)

involved_in neuromuscular junction development

inferred from mutant phenotype

(Cauchi et al., 2008)

involved_in protein-RNA complex assembly

inferred from mutant phenotype

(Shpargel et al., 2009)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in spliceosomal snRNP assembly

inferred from sequence or structural similarity with UniProtKB:Q9UHI6

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN002777080

(GO Reference Genome Project, 2011-)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

located_in Cajal body

inferred from direct assay

(Shpargel et al., 2009)

is_active_in cytoplasmic U snRNP body

inferred from direct assay

(Cauchi et al., 2010)

located_in cytoplasm

inferred from direct assay

(Shpargel et al., 2009)

located_in Gemini of Cajal bodies

inferred from direct assay

(Cauchi, 2011)

part_of SMN complex

inferred from direct assay

(Matera et al., 2019)

inferred from mutant phenotype

(Borg et al., 2015)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of SMN complex

inferred from biological aspect of ancestor with PANTHER:PTN002777080

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

SURVIVAL MOTOR NEURON COMPLEX - GEM4A VARIANT

GEMINS

DEAD-BOX RNA HELICASES

SURVIVAL MOTOR NEURON COMPLEX - GEM4B VARIANT

SURVIVAL MOTOR NEURON COMPLEX - GEM4C VARIANT

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

RNA helicase activity

ATP + H2O = ADP + phosphate + H(+) (3.6.4.13)

Summaries

Gene Snapshot

Gene Group (FlyBase)

SURVIVAL MOTOR NEURON COMPLEX - GEM4A VARIANT -: The survival motor neuron (SMN) complex is minimally composed of SMN and Gemin proteins. The SMN complex mediates the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs). (Adapted from FBrf0228762).
GEMINS -: Gemin proteins are found in the nuclear Gemini of coiled bodies or in U bodies. Gemins interact notably with Smn to form the SMN complex. (Adapted from FBrf0223758).
DEAD-BOX RNA HELICASES -: DEAD-box RNA helicases belong to helicase superfamily 2 (SF2). They are characterized by the presence of nine conserved helicase motifs, including the DEAD sequence in motif II. (Adapted from PMID:21509200.)
SURVIVAL MOTOR NEURON COMPLEX - GEM4B VARIANT -: The survival motor neuron (SMN) complex is minimally composed of SMN and Gemin proteins. The SMN complex mediates the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs). (Adapted from FBrf0228762).
SURVIVAL MOTOR NEURON COMPLEX - GEM4C VARIANT -: The survival motor neuron (SMN) complex is minimally composed of SMN and Gemin proteins. The SMN complex mediates the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs). (Adapted from FBrf0228762).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Gem3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V3C4)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Gem3-RA

FBtr0076292

NM_079295

3423

1028

Gem3-RB

FBtr0334100

NM_001274749

3963

1028

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Gem3-PA

FBpp0076021

116.5

1028

7.16

FBpp0306223

116.5

1028

7.16

Q9V3C4

NP_001261678

AGB94372

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1028 aa isoforms: Gem3-PA, Gem3-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Gem3 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Gem3_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.17

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis

oocyte

(Cauchi et al., 2010)

nurse cell

(Cauchi et al., 2010)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in Cajal body

inferred from direct assay

(Shpargel et al., 2009)

is_active_in cytoplasmic U snRNP body

inferred from direct assay

(Cauchi et al., 2010)

located_in cytoplasm

inferred from direct assay

(Shpargel et al., 2009)

located_in Gemini of Cajal bodies

inferred from direct assay

(Cauchi, 2011)

part_of SMN complex

inferred from direct assay

(Matera et al., 2019)

inferred from mutant phenotype

(Borg et al., 2015)

Expression Deduced from Reporters

Reporter: P{PZ}Gem3^rL562

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

eye disc

(Meister and Braun, 1995.10)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Gem3 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Gem3

View exonic expression by tissue for Dmel\Gem3

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39195/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0011802

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0011802

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0011802

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6539

Flygut - An atlas of the Drosophila adult midgut

FBgn0011802

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Gem3

Transgenic constructs containing regulatory region of Gem3

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene not disrupted in

Df(3L)AC1

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(3L)lxd6

(Spradling et al., 1999)

Gene disrupted in

Df(3L)ED4457

(Shpargel et al., 2009, Cauchi et al., 2008)

Inferred from location ( 2 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Gem3^GD17221, Scer\GAL4^Mef2.PR

Gem3^ΔN.UAS

lethal, with Gem3^ΔN.UAS, Scer\GAL4^Mef2.PR

Gem3^GD17221

lethal, with Scer\GAL4^1032.hx

Gem3^GD17221

lethal, with Scer\GAL4^Act5C.PU

lethal, with Scer\GAL4^c179

lethal, with Scer\GAL4^da.PU

lethal, with Scer\GAL4^elav.PU

Gem3^ΔN.UAS

lethal, with Scer\GAL4^how-24B

lethal, with Scer\GAL4^Tub.PU

Gem3^GD17221

lethal (with Df(3L)ED4457)

lethal (with Gem3^e03688)

Gem3^rL562

lethal (with Gem3^rL562)

Gem3^e03688

lethal | heat sensitive, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PU

Gem3^GD17221

lethal | heat sensitive, with Dcr-2^UAS.cDa, Scer\GAL4^Mef2.PR

Gem3^GD17221

lethal | heat sensitive, with Scer\GAL4^G7

Gem3^ΔN.UAS

lethal | heat sensitive, with Scer\GAL4^RapGAP1-OK6

Gem3^GD17221

lethal | recessive

lethal - all die before end of larval stage (with Gem3^e03688)

Gem3^rL562

lethal - all die before end of larval stage (with Gem3^rL562)

Gem3^e03688

lethal - all die before end of larval stage | recessive

partially lethal | heat sensitive, with Scer\GAL4^C57

Gem3^ΔN.UAS

partially lethal | heat sensitive, with Scer\GAL4^Mef2.PR

Gem3^ΔN.UAS

partially lethal - majority die, with Dcr-2^UAS.cDa, Scer\GAL4^Mef2.PR

Gem3^GD17221

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Gem3^GD1417

partially lethal - majority die | heat sensitive, with Scer\GAL4^G7

Gem3^GD17221

partially lethal - majority die | heat sensitive, with Scer\GAL4^nSyb.PS

Gem3^GD17221

partially lethal - majority live, with Scer\GAL4^G7

Gem3^GD17221

partially lethal - majority live | heat sensitive, with Scer\GAL4^elav.PU

Gem3^GD17221

viable

Gem3^B-rev

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Gem3^GD17221

viable, with Scer\GAL4^C57

Gem3^GD17221

viable, with Scer\GAL4^elav-C155

viable, with Scer\GAL4^GMR.PU

Gem3^GD17221

viable, with Scer\GAL4^insc-Mz1407

Gem3^GD17221

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^Mhc.PW

Gem3^GD17221

viable, with Scer\GAL4^nrv2.PS

viable, with Scer\GAL4^pnr-MD237

Gem3^GD17221

viable, with Scer\GAL4^RapGAP1-OK6

viable, with Scer\GAL4^tin.CΔ4

Gem3^GD17221

viable, with Scer\GAL4^Toll-6-D42

Other Phenotypes

Allele

abnormal developmental rate | larval stage

Gem3^rL562

abnormal flight, with Gem3^GD17221, Gem3^UAS.cCa, Scer\GAL4^Mef2.PR

Gem3^ΔN.UAS

abnormal flight, with Gem3^ΔN.UAS, Gem3^GD17221, Scer\GAL4^Mef2.PR

Gem3^UAS.cCa

abnormal flight, with Gem3^ΔN.UAS, Gem3^UAS.cCa, Scer\GAL4^Mef2.PR

Gem3^GD17221

abnormal flight, with Scer\GAL4^1032.hx

Gem3^GD17221

abnormal flight, with Scer\GAL4^Mef2.PR

Gem3^ΔN.UAS

abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4^Mef2.PR

abnormal locomotor behavior | larval stage

abnormal locomotor behavior | third instar larval stage (with Gem3^e03688)

Gem3^rL562

abnormal locomotor behavior | third instar larval stage (with Gem3^rL562)

Gem3^e03688

abnormal neuroanatomy | larval stage, with Scer\GAL4^1032.hx

Gem3^UAS.cCa

abnormal neuroanatomy | larval stage (with Gem3^e03688)

Gem3^rL562

abnormal neuroanatomy | larval stage (with Gem3^rL562)

Gem3^e03688

decreased body size | larval stage

flightless, with Scer\GAL4^Mef2.PR

Gem3^ΔN.UAS

flightless | progressive, with Scer\GAL4^Mef2.PR

Gem3^GD17221

flightless | progressive | conditional, with Scer\GAL4^Mef2.PR

Gem3^ΔN.UAS

short lived, with Scer\GAL4^Mef2.PR

Gem3^GD17221

short lived | conditional, with Scer\GAL4^Mef2.PR

Gem3^ΔN.UAS

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

Gem3^GD17221

some die during larval stage (with Gem3^e03688)

Gem3^rL562

some die during larval stage (with Gem3^rL562)

Gem3^e03688

some die during larval stage | recessive

some die during pupal stage, with Scer\GAL4^pnr-MD237

Gem3^GD1417

visible, with Scer\GAL4^pnr-MD237

Gem3^GD17221

Phenotype manifest in

Allele

anterior pupal spiracle (with Gem3^e03688)

Gem3^rL562

anterior pupal spiracle (with Gem3^rL562)

Gem3^e03688

embryonic/larval neuromuscular junction (with Gem3^e03688)

Gem3^rL562

embryonic/larval neuromuscular junction (with Gem3^rL562)

Gem3^e03688

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^1032.hx

Gem3^UAS.cCa

head | pupal stage, with Scer\GAL4^how-24B

Gem3^ΔN.UAS

indirect flight muscle cell, with Scer\GAL4^Mef2.PR

Gem3^ΔN.UAS

leg | pupal stage, with Scer\GAL4^how-24B

Gem3^ΔN.UAS

puparium cuticle (with Gem3^e03688)

Gem3^rL562

puparium cuticle (with Gem3^rL562)

Gem3^e03688

trichogen cell, with Scer\GAL4^pnr-MD237

Gem3^GD17221

wing | pupal stage, with Scer\GAL4^how-24B

Gem3^ΔN.UAS

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (20)

Hsap\DDX20

8 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (10)

Rnor\Ddx20

8 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (13)

Mmus\Ddx20

8 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (13)

Xtro\ddx20

3 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (14)

Drer\ddx20

8 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (20)

Cele\mel-46

8 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (15)

Agam\AgaP_AGAP005391

7 of 12

Yes

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (31)

Atha\AT1G51380

2 of 13

Yes

Atha\AT1G72730

2 of 13

Yes

Atha\EIF4A1

2 of 13

Yes

Atha\EIF4A-2

2 of 13

Yes

Atha\EIF4A-III

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (12)

Scer\FAL1

2 of 13

Yes

Scer\TIF1

2 of 13

Yes

Scer\TIF2

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (5)

Spom\SPAC1F5.10

2 of 12

Yes

Spom\SPAC1006.07

2 of 12

Yes

Spom\ddx27

1 of 12

Spom\ste13

1 of 12

Spom\uap56

1 of 12

Escherichia coli (enterobacterium) (4)

Ecol\deaD

1 of 11

Yes

Ecol\mfd

1 of 11

Yes

Ecol\recG

1 of 11

Yes

Ecol\rhlE

1 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Gem3. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (30)

CG7483

4 of 13

abs

3 of 13

bel

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

vas

3 of 13

ais

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Rs1

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

pit

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Gem3^ΔN.UAS

exacerbates childhood spinal muscular atrophy

modeled by Smn^{RNAi.C.UAS.WIZ}

(Borg et al., 2015)

modeled by Smn^{RNAi.N.UAS.WIZ}

(Borg et al., 2015)

exacerbates amyotrophic lateral sclerosis type 10

modeled by Hsap\TARDBP^WT.UAS

(Cacciottolo et al., 2019)

modeled by Hsap\TARDBP^UAS.Tag:FLAG

(Cacciottolo et al., 2019)

modeled by Hsap\TARDBP^WT.UAS.GFP

(Cacciottolo et al., 2019)

modeled by TBPH^{UAS.10x.Venus}

(Cacciottolo et al., 2019)

exacerbates amyotrophic lateral sclerosis type 6

modeled by caz^{UAS.x10.Tag:FLAG}

(Cacciottolo et al., 2019)

modeled by Hsap\FUS^{UAS.10x.Tag:FLAG}

(Cacciottolo et al., 2019)

modeled by Hsap\FUS^{P525L.10x.UAS.Tag:FLAG}

(Cacciottolo et al., 2019)

modeled by Hsap\FUS^{P525L.UAS.RFP(Unk),Tag:HA}

(Cacciottolo et al., 2019)

modeled by caz^VSH330388

(Cacciottolo et al., 2019)

modeled by caz^KK107486

(Cacciottolo et al., 2019)

exacerbates amyotrophic lateral sclerosis type 1

modeled by Sod1ⁿ¹

(Cacciottolo et al., 2019)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

DDX20; DEAD-box helicase 20

8 of 14

606168

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Gem3

two hybrid

(Cacciottolo et al., 2019)

Gem3 - Gem2

experimental knowledge based

(Guruharsha et al., 2011)

Gem3 - Glos

experimental knowledge based

(Guruharsha et al., 2011)

Gem3 - Sbat

two hybrid

(Lanfranco et al., 2017)

Gem3 - Smn

anti bait coimmunoprecipitation, anti tag western blot, pull down, experimental knowledge based, anti tag coimmunoprecipitation, western blot, two hybrid, Identification by mass spectrometry

(Maccallini et al., 2020, Borg et al., 2016, Praveen et al., 2014, Guruharsha et al., 2011, Shpargel et al., 2009, Cauchi et al., 2008)

Gem3 - Tgs1

two hybrid, anti tag coimmunoprecipitation, Identification by mass spectrometry

(Maccallini et al., 2020, Borg et al., 2016)

Gem3 - icln

two hybrid

(Borg et al., 2016)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

icln

enhanceable

Gem3

(Borg et al., 2016)

icln

suppressible

Gem3

(Borg et al., 2016)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

64576

DroID - A comprehensive database of gene and protein interactions.

FBgn0011802

MIST (genetic) - An integrated Molecular Interaction Database

39195

MIST (protein-protein) - An integrated Molecular Interaction Database

39195

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-33

Cytogenetic map

67E3-67E3

Sequence location

3L:10,459,081..10,463,101 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

67E3-67E3

Limits computationally determined from genome sequence between P{lacW}l(3)L0539^L0539&P{PZ}Dhh1^rL562 and P{PZ}simj⁰¹⁸¹⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

67E1-67E4

(determined by in situ hybridisation)

(Spradling et al., 1999)

67E1-67E4

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

3-33

(FlyBase, 2016)

3-25.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

71633

w¹¹¹⁸; P{VT028607-p65.AD}attP40

75182

w¹¹¹⁸; P{VT028607-GAL4.DBD}attP2

80887

y¹, v¹; P{TKO.GS04791}attP40

12079

P{PZ}Gem3^rL562 ry⁵⁰⁶/TM3, Sb¹

33408

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00287}attP2

58532

y¹ w^*; Mi{MIC}Gem3^MI12548/TM3, Sb¹ Ser¹

604029

w¹¹¹⁸; L^*/CyO; P{SX4.lexA::HG}Gem3^S40-1/TM6B, Tb¹

Korea

12424

P{VT028607-GAL4}attP2

NIG-Fly

HMJ22280

y¹ v¹; P{TRiP.HMJ22280}attP40/CyO

HMS00287

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00287}attP2

VDRC

v49505

w¹¹¹⁸ P{GD17221}v49505

v49506

w¹¹¹⁸; P{GD17221}v49506

Genomic Clones (21)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (34)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD05563

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0011802

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0011802

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Na et al., 2016)

polyclonal

(Cauchi et al., 2010)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

FlyBase curator comment: The gene corresponding to the CG6539 annotation was previously named "Dhh1" in FlyBase based on information in FBrf0125024, where the yeast Dhh1 protein is given as a homolog of CG6539. However, although the CG6539 does show some similarity to the yeast Dhh1 protein, the Drosophila ortholog of Dhh1 is the CG4916 annotation. Thus, to avoid confusion, the gene corresponding to the CG6539 annotation has been renamed to "Gem3, Gemin3" in FlyBase, as suggested in FBrf0206864.

(Shpargel et al., 2009)

Etymology

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

BcDNA.LD05563

(Tsang, 1999.6.19)

BcDNA:LD05563

CG6539

(Matera et al., 2019, Çiçek et al., 2016, Na et al., 2016, Hosono et al., 2015, Cauchi et al., 2010, Ni et al., 2010.12.1, Shpargel et al., 2009, Cauchi et al., 2008, Gandhi et al., 2007)

DHH1

(Boudet et al., 2001)

Dhh1

(Cauchi et al., 2008, Kroiss et al., 2008)

DmRH22

(Boudet et al., 2001)

GEMIN3

(Meister, 2013)

Gem3

(Farrugia et al., 2022, Maccallini et al., 2020, Taschuk et al., 2020, Matera et al., 2019, Lanfranco et al., 2017, Transgenic RNAi Project members, 2017-, Borg et al., 2016, Na et al., 2016, Borg et al., 2015, Hsu et al., 2015, Moy et al., 2014, Olesnicky et al., 2014, Borg and Cauchi, 2013, Moshkin et al., 2013, Shpargel et al., 2009)

Gemin3

(Glineburg and Nguyen, 2025)

dGem3

(Praveen et al., 2014, Shpargel et al., 2009)

gemin3

(Lloyd and Taylor, 2010)

l(3)rL562

(Beaton, 2000.1.31, Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)

Name Synonyms

DEAD/DEAH RNA helicase 1

(Spradling et al., 1999)

Gemin 3

Gemin3

(Dyson et al., 2025, Matera et al., 2019, Borg and Cauchi, 2013, Buckingham and Liu, 2011, Cauchi, 2011, Grice and Liu, 2011, Cauchi et al., 2010, Ulvila et al., 2010, Kroiss et al., 2009, Shpargel et al., 2009, Kroiss et al., 2008)

dGemin3

(Shpargel et al., 2009)

gemin3

(Cauchi et al., 2008)

Secondary FlyBase IDs

FBgn0027895

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 37 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39195

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V3C4

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q9V3C4

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V3C4

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39195/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0011802

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0011802

FlyMine - An integrated database for Drosophila genomics

FBgn0011802

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG6539

MARRVEL_MODEL - MARRVEL (model organism gene)

39195

Linkouts

BioGRID - A database of protein and genetic interactions.

64576

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0011802

DroID - A comprehensive database of gene and protein interactions.

FBgn0011802

DRSC - Results frm RNAi screens

FBgn0011802

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Gem3_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0011802

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0011802

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6539

Flygut - An atlas of the Drosophila adult midgut

FBgn0011802

MIST (genetic) - An integrated Molecular Interaction Database

39195

MIST (protein-protein) - An integrated Molecular Interaction Database

39195

References (71)

The following external sites may use different assemblies or annotations than FlyBase.

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