FlyBase Gene Report: Dmel\ort

head

(Gengs et al., 2002)

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

eye

(Xu et al., 2004)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult optic lobe

(Witte et al., 2002)

adult ventral nerve cord

(Witte et al., 2002)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism

(Gisselmann et al., 2002)

prepupal stage -- adult stage

organism

(Gisselmann et al., 2002)

adult head

(Iovchev et al., 2006, Gisselmann et al., 2002)

adult thorax & adult abdomen

Comment: reference states "adult body"

(Gisselmann et al., 2002)

adult brain

(Pantazis et al., 2008)

neuron

(Pantazis et al., 2008)

lamina monopolar neuron

(Pantazis et al., 2008)

(Karuppudurai et al., 2014)

adult stage | male

testis

(Iovchev et al., 2006)

Additional Descriptive Data

ort expression has been analyzed via RT-PCR on isolated Tm5c cell bodies which had been labeled with ScerGAL4^ort.C1a.

(Karuppudurai et al., 2014)

ort is expressed in ocellar postsynaptic L-type interneurons.

(Hong et al., 2006)

ort transcript is observed mainly in the head and testis. Transcript levels are relatively low in headless female adults, and headless, gonadless male adults.

(Iovchev et al., 2006)

Eye-enriched transcripts determined by ratio of expression level in wild-type heads. versus expression level in so heads.

(Xu et al., 2004)

ort transcript cannot be detected in eggs or larvae using RT-PCR. Although RT-PCR demonstrates the presence of ort transcript in adult heads, this expression is not detectable by in situ hybridization.

(Gisselmann et al., 2002)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

ort is prominently expressed in ocellar postsynaptic L-type interneurons.

(Hong et al., 2006)

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of histamine-gated chloride channel complex

inferred from direct assay

(Zheng et al., 2002)

Expression Deduced from Reporters

Reporter: P{ort-GAL4.3126}

Stage

Tissue/Position (including subcellular localization)

Reference

lamina monopolar neuron L2

(Pantazis et al., 2008)

(Pantazis et al., 2008)

lamina monopolar neuron L3

(Pantazis et al., 2008)

medulla layer M1

(Pantazis et al., 2008)

medulla layer M2

(Pantazis et al., 2008)

medulla layer M3

(Pantazis et al., 2008)

medulla layer M5

(Pantazis et al., 2008)

lamina | restricted

(Pantazis et al., 2008)

Reporter: P{ort-GAL4.C1-3}

Stage

Tissue/Position (including subcellular localization)

Reference

medulla layer M10 | restricted

medulla layer M1 | restricted

(Gao et al., 2008)

medulla layer M2 | restricted

(Gao et al., 2008)

medulla layer M3 | restricted

(Gao et al., 2008)

medulla layer M6 | restricted

(Gao et al., 2008)

medulla layer M8 | restricted

(Gao et al., 2008)

(Gao et al., 2008)

lobula layer 1 | restricted

(Gao et al., 2008)

lobula layer 2 | restricted

(Gao et al., 2008)

lobula layer 5 | restricted

(Gao et al., 2008)

T neuron | subset

(Gao et al., 2008)

lamina monopolar neuron L2

(Gao et al., 2008)

(Gao et al., 2008)

transmedullary neuron Tm2

(Gao et al., 2008)

transmedullary neuron Tm5

(Gao et al., 2008)

transmedullary neuron Tm9

(Gao et al., 2008)

medulla intrinsic columnar neuron | subset

(Gao et al., 2008)

T2 neuron

(Gao et al., 2008)

(Gao et al., 2008)

glial cell | subset

(Gao et al., 2008)

medulla | restricted

(Gao et al., 2008)

lamina | restricted

(Gao et al., 2008)

Reporter: P{ort-GAL4.C1-4}

Stage

Tissue/Position (including subcellular localization)

Reference

Reporter: P{ort-GAL4.C1}

Stage

Tissue/Position (including subcellular localization)

Reference

transmedullary neuron Tm5

neuron | subset of medulla

(Li et al., 2013)

(Li et al., 2013)

transmedullary Y neuron TmY10

(Li et al., 2013)

(Li et al., 2013)

Reporter: P{ort-Gal4.C1a.DBD}

Stage

Tissue/Position (including subcellular localization)

Reference

transmedullary neuron Tm5a

Comment: when combined with P{dVP16AD}ET18k

when combined with P{dVP16AD}ET24g

(Karuppudurai et al., 2014, Melnattur et al., 2014)

transmedullary neuron Tm5b

Comment: when combined with P{dVP16AD}ET18k

when combined with P{dVP16AD}ET24g

(Karuppudurai et al., 2014, Melnattur et al., 2014)

Comment: when combined with P{ET.VP16.AD}tou^9A30

when combined with P{dVP16AD}ET9A

(Melnattur et al., 2014, Ting et al., 2014)

Comment: when combined with P{dVP16AD}VGlut^{OK371-dVP16AD}

when combined with P{dVP16AD}VGlut^{OK371-dVP16AD} (combination referred to as 'ortC1a∩ ok371-dVP16')

(Sancer et al., 2019, Melnattur et al., 2014)

Reporter: P{ort-GAL4.C1a}

Stage

Tissue/Position (including subcellular localization)

Reference

Reporter: P{ort-GAL4.C2}

Stage

Tissue/Position (including subcellular localization)

Reference

Reporter: P{ort-GAL4.C2b}

Stage

Tissue/Position (including subcellular localization)

Reference

lamina monopolar neuron L2 | subset

(Karuppudurai et al., 2014)

distal medullary amacrine neuron Dm-DRA2

(Sancer et al., 2019, Karuppudurai et al., 2014)

(Sancer et al., 2019)

Reporter: P{ort-GAL4.C3}

Stage

Tissue/Position (including subcellular localization)

Reference

lamina monopolar neuron L2

(Gao et al., 2008)

transmedullary neuron Tm2

(Gao et al., 2008)

transmedullary neuron Tm9

(Gao et al., 2008)

centrifugal neuron C2

(Gao et al., 2008)

medulla intrinsic neuron Mi1

(Gao et al., 2008)

Reporter: P{ort-GAL4.DBD.C1-3}

Stage

Tissue/Position (including subcellular localization)

Reference

adult optic lobe neuron | subset

Comment: when combined with P{dVP16AD}VGlut^{OK371-dVP16AD}

(Bahl et al., 2015, Gao et al., 2008)

Comment: when combined with P{dVP16AD}VGlut^{OK371-dVP16AD}

(Gao et al., 2008)

Comment: when combined with P{dVP16AD}VGlut^{OK371-dVP16AD}

(Gao et al., 2008)

Comment: when combined with P{dVP16AD}VGlut^{OK371-dVP16AD}

(Gao et al., 2008)

Reporter: P{ort-GAL4.DBD.C2}

Stage

Tissue/Position (including subcellular localization)

Reference

pharate adult stage P8 -- P15

Comment: when combined with P{dVP16AD}VGlut^{OK371-dVP16AD}

(Ting et al., 2014)

distal medullary amacrine neuron Dm8a | subset

Comment: when combined with P{dVP16AD}VGlut^{OK371-dVP16AD}

(Gao et al., 2008)

glial cell | subset

Comment: when combined with P{dVP16AD}VGlut^{OK371-dVP16AD}

(Gao et al., 2008)

Comment: when combined with P{dVP16AD}VGlut^{OK371-dVP16AD}

(Gao et al., 2008)

Comment: when combined with P{dVP16AD}VGlut^{OK371-dVP16AD}

(Ting et al., 2014)

Reporter: P{ort-LexA.C1a.DBD}

Stage

Tissue/Position (including subcellular localization)

Reference

Comment: when combined with P{dVP16AD}ET24a

(Melnattur et al., 2014)

Comment: when combined with P{dVP16AD}VGlut^{OK371-dVP16AD}

(Karuppudurai et al., 2014)

Reporter: P{ort-lexA.C1a.VP16}

Stage

Tissue/Position (including subcellular localization)

Reference

transmedullary neuron Tm5a

(Ting et al., 2014)

transmedullary neuron Tm5b

(Ting et al., 2014)

(Ting et al., 2014)