FlyBase Gene Report: Dmel\ImpE1

General Information

Symbol

Dmel\ImpE1

Species

D. melanogaster

Name

Ecdysone-inducible gene E1

Annotation Symbol

CG32356

Feature Type

protein_coding_gene

FlyBase ID

FBgn0001253

Gene Model Status

Current

Stock Availability

5 publicly available

Gene Summary

Ecdysone-inducible gene E1 (ImpE1) encodes a protein similar to a low-density lipoprotein (LDL) receptor. It is hypothesized to play a role in the cell rearrangements associated with morphogenesis of the disc epithelium. ImpE1 transcription is upregulated by 20-hydroxyecdysone. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Also Known As

IMP-E1

Key Links

Genomic Location

Cytogenetic map

66C8-66C10

Sequence location

3L:8,372,405..8,391,171 [+]

Recombination map

3-24

RefSeq locus

NT_037436 REGION:8372405..8391171

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (2 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in imaginal disc eversion

traceable author statement

(Andres et al., 1993)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in extrinsic component of membrane

traceable author statement

(Andres et al., 1993)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Imp-E: Inducible membrane-bound polysomes-Early

Name given to three genes encoding transcripts associated with membrane-bound polysomes in imaginal discs and expressed only in response to 20-hydroxyecdysone (20 HOE). Expression studied both in vitro and in vivo. Imp-E denotes three "early" genes involved in elongation of appendage-forming regions of the imaginal discs by means of epithelial-cell rearrangements (Natzle et al., 1988).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\ImpE1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q7KUB3)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

ImpE1-RA

FBtr0076606

NM_080110

6043

1616

ImpE1-RB

FBtr0076607

NM_168270

919

146

ImpE1-RC

FBtr0333057

NM_001274648

6099

1616

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

ImpE1-PA

FBpp0076333

177.0

1616

3.74

FBpp0076334

16.0

146

8.15

FBpp0305271

177.0

1616

3.74

Q7KUB3

NP_001261577

AGB94272

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1616 aa isoforms: ImpE1-PA, ImpE1-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ImpE1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ImpE1_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.46

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 9 -- 10

inclusive hindgut primordium

(Fisher et al., 2012)

embryonic stage 11 -- 12

hindgut proper primordium

(Fisher et al., 2012)

embryonic stage 12 -- 16

embryonic/larval tracheal section

(Chung et al., 2011)

embryonic stage 13 -- 16

larval foregut atrium

(Fisher et al., 2012)

embryonic dorsal epidermis

(Fisher et al., 2012)

embryonic esophagus

(Fisher et al., 2012)

embryonic foregut

(Fisher et al., 2012)

embryonic head epidermis

(Fisher et al., 2012)

embryonic hindgut

(Fisher et al., 2012)

embryonic ventral epidermis

(Fisher et al., 2012)

embryonic/larval tracheal dorsal trunk

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

third instar - uncleared gut stage

larval central nervous system

(Restifo and Hauglum, 1998)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\ImpE1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\ImpE1

View exonic expression by tissue for Dmel\ImpE1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001253

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

45879/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001253

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001253

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001253

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG32356

Flygut - An atlas of the Drosophila adult midgut

FBgn0001253

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\ImpE1

Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of ImpE1

Transgenic constructs containing regulatory region of ImpE1

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 2 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Act5C.PU

ImpE1^KK111088

viable

ImpE1^f03112

viable, with Scer\GAL4^Mef2.PR

ImpE1^GD4812

viable, with Scer\GAL4^pnr-MD237

ImpE1^GD4812

Sterility

Allele

fertile

ImpE1^f03112

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (16)

Hsap\CD320

1 of 14

Yes

Hsap\DGCR2

1 of 14

Yes

Hsap\EGF

1 of 14

Yes

Hsap\LDLRAD3

1 of 14

Yes

Hsap\LDLR

1 of 14

Yes

Hsap\LRP1B

1 of 14

Yes

Hsap\LRP1

1 of 14

Yes

Hsap\LRP3

1 of 14

Yes

Hsap\LRP4

1 of 14

Yes

Hsap\LRP5

1 of 14

Yes

Hsap\LRP6

1 of 14

Yes

Hsap\LRP8

1 of 14

Yes

Hsap\LRP10

1 of 14

Yes

Hsap\LRP12

1 of 14

Yes

Hsap\SORL1

1 of 14

Yes

Hsap\VLDLR

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (11)

Rnor\Cd320

1 of 14

Yes

Rnor\Ldlr

1 of 14

Yes

Rnor\Lrp1

1 of 14

Yes

Rnor\Lrp3

1 of 14

Yes

Rnor\Lrp4

1 of 14

Yes

Rnor\Lrp5

1 of 14

Yes

Rnor\Lrp6

1 of 14

Yes

Rnor\Lrp8

1 of 14

Yes

Rnor\Lrp12

1 of 14

Yes

Rnor\Sspo

1 of 14

Yes

Rnor\Vldlr

1 of 14

Yes

Mus musculus (laboratory mouse) (18)

Mmus\Cd320

1 of 14

Yes

Mmus\Dgcr2

1 of 14

Yes

Mmus\Egf

1 of 14

Yes

Mmus\Ldlrad3

1 of 14

Yes

Mmus\Ldlr

1 of 14

Yes

Mmus\Lrp1b

1 of 14

Yes

Mmus\Lrp1

1 of 14

Yes

Mmus\Lrp2

1 of 14

Yes

Mmus\Lrp3

1 of 14

Yes

Mmus\Lrp4

1 of 14

Yes

Mmus\Lrp5

1 of 14

Yes

Mmus\Lrp6

1 of 14

Yes

Mmus\Lrp8

1 of 14

Yes

Mmus\Lrp10

1 of 14

Yes

Mmus\Lrp12

1 of 14

Yes

Mmus\Sorl1

1 of 14

Yes

Mmus\Sspo

1 of 14

Yes

Mmus\Vldlr

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (7)

Xtro\camk2b

1 of 13

Yes

Xtro\emx2

1 of 13

Yes

Xtro\fbxw7

1 of 13

Yes

Xtro\frmd4b

1 of 13

Yes

Xtro\gria1

1 of 13

Yes

Xtro\tmed1

1 of 13

Yes

Xtro\ubtf

1 of 13

Yes

Danio rerio (Zebrafish) (15)

Drer\dgcr2

1 of 14

Yes

Drer\ldlrad3

1 of 14

Yes

Drer\ldlra

1 of 14

Yes

Drer\LOC100536757

1 of 14

Yes

Drer\lrp1ab

1 of 14

Yes

Drer\lrp1bb

1 of 14

Yes

Drer\lrp2b

1 of 14

Yes

Drer\lrp3

1 of 14

Yes

Drer\lrp5

1 of 14

Yes

Drer\lrp8

1 of 14

Yes

Drer\lrp10

1 of 14

Yes

Drer\lrp13

1 of 14

Yes

Drer\sorl1

1 of 14

Yes

Drer\tmprss3b

1 of 14

Yes

Drer\vldlr

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (12)

Cele\F32E10.8

2 of 14

Yes

Cele\clec-180

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (16)

Agam\AgaP_AGAP006069

7 of 12

Yes

Agam\AgaP_AGAP010896

2 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\REA1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:ImpE1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (13)

CG7381

2 of 13

CG11674

2 of 13

LpR2

2 of 13

arr

1 of 13

CG6553

1 of 13

CG6739

1 of 13

CG32432

1 of 13

cue

1 of 13

Culd

1 of 13

Lrp4

1 of 13

mgl

1 of 13

trol

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

69948

DroID - A comprehensive database of gene and protein interactions.

FBgn0001253

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-24

Cytogenetic map

66C8-66C10

Sequence location

3L:8,372,405..8,391,171 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

66C8-66C10

Limits computationally determined from genome sequence between P{lacW}l(3)L0139^L0139 and P{PZ}Gug⁰³⁹²⁸&P{EP}CG6745^EP595b

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

66C7-66C10

(Robertson, 1999.12.17)

66C-66C

(determined by in situ hybridisation)

(Natzle et al., 1986)

Experimentally Determined Recombination Data

Location

3-24

(FlyBase, 2016)

3-19.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

93467

w^*; TI{CRIMIC.TG4.0}ImpE1^{CR60125-TG4.0}/TM3, Sb¹

32356R-2

32356R-3

v104613

v12854

w¹¹¹⁸; P{GD4812}v12854

Genomic Clones (24)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (51)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0001253

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0001253

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

ImpE1 is expressed in response to both ecdysone and pattern formation genes and may play a part in the morphogenetic events of embryogenesis and imaginal disc eversion.

(Clark et al., 1995)

Down regulation of ImpE1 transcription is altered in Eip74EF mutants.

(Fletcher and Thummel, 1995)

ImpE1 and Dfd have been examined for their positions relative to the Broad complex genes in the hormone-regulated pathway of CNS metamorphosis. Activity of any individual Broad complex subfunction is not required for ImpE1 induction.

(Restifo et al., 1995)

Ecdysteroid-regulated gene.

(Andres et al., 1993)

The sequential temporal expression of steroid hormone-responsive genes in imaginal discs may be important in organizing cellular mechanisms involved in morphogenesis of the epithelium.

(Natzle, 1993)

Gene encoding a transcript associated with membrane-bound polysomes in imaginal discs and expressed only in response to 20-hydroxyecdysone (20 HOE). Expression studied both in vitro and in vivo. One of the 'early' genes involved in elongation of appendage-forming regions of the imaginal discs by means of epithelial-cell rearrangements (Natzle, Fristrom and Fristrom, 1988).

Relationship to Other Genes

Source for database merge of

Source for merge of: ImpE1 CG7116 CG13668

(Natzle, 2000.5.4)

Source for merge of: ImpE1 BcDNA:RE39082

(FlyBase, 1996-)

Additional comments

Annotations CG13668, CG7116 merged as CG32356 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Source for merge of ImpE1 BcDNA:RE39082 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

BcDNA:RE39082

CG13668

(Natzle, 2000.5.4)

CG32356

(Hosono et al., 2015, Heinrichsen et al., 2014, Keleman et al., 2009.8.5, Lebo et al., 2009)

CG7116

(Harris et al., 2003, Natzle, 2000.5.4)

CT22003

(Hynes and Zhao, 2000)

CT33103

(Hynes and Zhao, 2000)

Imp-E1

(Bialecki et al., 2002, Bayer et al., 1996, Fletcher and Thummel, 1995, Restifo et al., 1995, Andres et al., 1993, Natzle, 1993)

ImpE1

(Li et al., 2024, Garaulet et al., 2021, Araki et al., 2020, Cho et al., 2020, Gene Disruption Project members, 2018-, Leow et al., 2018, Ou et al., 2016, Xie et al., 2015, Butchar et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Chung et al., 2011, Ou et al., 2011, Kong et al., 2010, Curtis et al., 2007)

Name Synonyms

Ecdysone-inducible gene E1

(Lebo et al., 2009)

Y1-like

(Hynes and Zhao, 2000)

Secondary FlyBase IDs

FBgn0035884
FBgn0035885
FBgn0052356
FBgn0062446

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 41 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

45879

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q7KUB3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q7KUB3

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0001253

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

45879/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0001253

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0001253

FlyMine - An integrated database for Drosophila genomics

FBgn0001253

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG32356

MARRVEL_MODEL - MARRVEL (model organism gene)

45879

Linkouts

BioGRID - A database of protein and genetic interactions.

69948

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0001253

DroID - A comprehensive database of gene and protein interactions.

FBgn0001253

DRSC - Results frm RNAi screens

FBgn0001253

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ImpE1_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0001253

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0001253

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG32356

Flygut - An atlas of the Drosophila adult midgut

FBgn0001253

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC034460

References (88)

The following external sites may use different assemblies or annotations than FlyBase.

-0.46

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