FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Clone: Dmel\GH16214
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General Information
Symbol
Dmel\GH16214
Species
D. melanogaster
Name
GH16214
FlyBase ID
FBcl0103591
Feature type
cDNA_clone
Computed gene(s)
Dmel\Mbs
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
    Library
    GH_cDNA
    • GH cDNA library of D. melanogaster, iso-1 strain, adult head.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      1,892
      NCBI
      AY051508
      Full Length sequence dataSequence Downloader
      ATCATCTCAGTTCGATTCAGTTCAGATCACCCAAACCCAGCTAATCCAATCAAACAAGTCTAAATCTCAATTTTCCATCA
GCGAGATGTCCTTTCGCTCGAGATCAAGAACCCAGGCGCCCCTGTCCAGTCGGCGGCGATCGTTGTCCTCCAGTTCGCGA
ATGGCTCCATCAAGTGGTGGTTCCGGGGCCTATAACTACAACGGAAGTAGCTATAGCAGCTCCGGCAGCACTGGTCGTCC
CCTGAGCACGGGCTACTTTCCCAGCAGCAGTAGCGCCTCCAGTTACCAGAGTCCCTATGCCAGTGTGTACAGCTCGAGGG
AGAGCTTGTACGGCGGAGGAGGTGCTGGTCGGAGTTCTTATGGCAGCGGTGGTGGTGGATACGACAGTTCCAGGTACAAT
CCCAGTTCCTATTCCTCGGCGGGATCTTCGTACTCCACAAGCGATCGATATGTCAGCCCCTATTCGAGTAGTTATGATAA
GGGTGTGACCACCGCTTCGCTGACCTTCAAGTCGCCCGGTTTGAGTTCGTCCAATTCGTTCAAGAGTTCTCGTTTACTGA
AGACCAAATCACTGTCAGCATCAAATAGCAGCCTGAATGGTGCCTATGGTGGTCCATCCTCGAATGGGGTAACCAGTGCC
GGAGCCACTGTGGCCGCCATCGCAGCCACAAGGAGCAACTCTTTGCGGGAACAGGAGCGCAAGTCTCGCAATCGCACGCG
GTCGAAGAGTGCGGCACAGAGATCCATTAGTGCTTCCTCAGAGAAGAGTGAAGGATATGAAAGTGGCAGTGAGCGGACAT
CCCGTTCCCGGCTGGGCAGCACCGCGAGTACGGCCACCACCAGCGAGTCAAAGAGCTCCAGCAGCAATGACAAGACGGAG
AATGGCGATGGCATTGACTACAAGGCGCTCTGGGAAGCGGAAAAATTGGAGAACGATAAGCTGAGGCAGATGCTCAAGCA
GAAGGACGATGAAGCCGTCCAGACACGTGCAACACTCGAGAGATTCGCCAATGCCACAACGAAAAATTCACTATCTGAAC
TTGAGAAACGCGAAAGAAGAGCTATGGAACGCAAGCTTTCCGAGTTGGAAGAAGAGCTCAAGCTATTGCAGAAGCTAAAG
ACTGAGAACGATCGTCTGCGAGCCGAGAATCGGGCCCTAACGCGGGTCGTCTCCAAGCTGACCACCTCGGCTCAGAGTCA
GCTGGCCAAGAGCAAATAGGCGCAGATCAGAAAAGCATTTAGATTGCCCAGCACCAGCCCACATGCACCCCAAAATTCTC
ACGCCCCCTTGCTCACGAAGCACAATGTCGCGAATATCGTTATATCGTTTCTATTAGAACTGGAAAAGCATCGATGTTTT
TGCCAGGCCAACATCAATAACCAATAATCATCGATGTTTTTCTTGCTCCTGGTTCGTTACAATACAATACTAAACACACT
TCGATGTCGTTAAAAAAATTATATGGATATTTAAGCATAACCGAAAACACAATAACTAAATGTATCGTAGCTAAAATTAA
GTTCACATTGAGCTCATTGGCTTTGGACTGGTCACTGGTAATATAAAACTTTAACAATACTATATACTACTACTCCTTGG
GAGTTACTATATACTACGGGCCCTTTGATAGAATGGCAATCGTCAAAAGTAGTTAATTTTTTAATAGTAAAATATTAATC
ACACGCCTTGTTCGTTTTTGGTAATCTACGAAAATCGCTAGGTGCAATTGTTTCTAATTTGAAATCATATTATATGGTTA
CTACAGACCACTGTACGGTTGATAAGTATTTGTAAATCAAAAGTTAGCCCACTCTCAAATCAAAATAAAGCTCATCGAAA
CCAACGAGATCAAACATCAAGTCCCCACCACCAAAAAAAAAAAAAAAAAAAA
      Library Information (1)
      Library: GH_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.

      (Rubin et al., 2000)
      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

      (Rubin et al., 2000)
      Comment

      The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.

      (Rubin et al., 2000)
      Reported As
      Symbol Synonyms
      GH
      (Rubin et al., 2000)
      GH_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 2 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)