FlyBase Allele Report: Dmel\NaPi-III[Δloop7.UAS.GFP]

General Information

Symbol

Dmel\NaPi-III^{Δloop7.UAS.GFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0337966

Feature type

allele

Associated gene

Dmel\NaPi-III

Associated Insertion(s)

Carried in Construct

P{UAS-NaPi-III.Δloop7.GFP}

Key Links

Transgenic product class

inframe_deletion

(Ma et al., 2017)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Ma et al., 2017)

Mutagen

in vitro construct

(Ma et al., 2017)

Progenitor genotype

Carried in construct

P{UAS-NaPi-III.Δloop7.GFP}

Cytology

Description

UAS regulatory sequences drive expression of NaPi-III which lacks sequences encoding the loop7 domain (consists of amino acid residues 1-239 fused to the C-terminal 183 amino acids). The protein is tagged at the C-terminal end with GFP.

(Ma et al., 2017)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

NaPi-III

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav.PU

(Ma et al., 2017)

Phenotype Manifest In

embryonic/larval motor neuron | third instar larval stage, with Scer\GAL4^elav.PU

(Ma et al., 2017)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^elav.PU

(Ma et al., 2017)

terminal bouton | third instar larval stage, with Scer\GAL4^elav.PU

(Ma et al., 2017)

Detailed Description

Statement

Reference

Third instar larval neuromuscular junctions expressing NaPi-III^{Δloop7.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^elav.PU display significant decreases in length and in the number of terminal boutons, as compared to controls.

(Ma et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions