FlyBase Allele Report: Dmel\Kdm4A[KO]

General Information

Symbol

Dmel\Kdm4A^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0335403

Feature type

allele

Associated gene

Dmel\Kdm4A

Associated Insertion(s)

TI{TI}Kdm4A^KO

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Shalaby et al., 2017)

Mutagen

ends-out gene targeting

(Shalaby et al., 2017)

FLPase

(Shalaby et al., 2017)

I-SceI

(Shalaby et al., 2017)

Progenitor genotype

Associated Insertion(s)

TI{TI}Kdm4A^KO

Cytology

Description

The endogenous Kdm4A open reading frame has been replaced by a loxP cassette which contains both Disc\RFP^3xP3.PC and the kanamycin resistance gene.

(Shalaby et al., 2017)

Allele components

Component

Use(s)

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:7,923,187..7,924,916 [-]

Comment:

The Kdm4A ORF was replaced with a knock-in cassette containing a Red Fluorescent Protein (RFP) transgene. Replaced region determined from reported primer sequences.

(Shalaby et al., 2017)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm | adult stage | dominant

(Lv et al., 2024)

suppressor of variegation, with In(1)w^m4

(Shalaby et al., 2017)

suppressor of variegation, with T(2;3)Sb^V

(Shalaby et al., 2017)

viable

(Shalaby et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

Kdm4A^KO mutants are viable and fertile and do not display any developmental timing defects. Kdm4A^KO suppresses the variegated phenotype of the w or Sb locus in the In(1)w^m4 or T(2;3)Sb^V background, respectively.

(Shalaby et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Kdm4A[+]/Kdm4A^KO is an enhancer of visible | adult stage phenotype of Scer\GAL4^C-765, smo^{5A.UAS.Tag:FLAG}

(Shalaby et al., 2017)

NOT Enhancer of

Statement

Reference

Kdm4A[+]/Kdm4A^KO is a non-enhancer of abnormal circadian rhythm | adult stage | dominant phenotype of CoRest^MI08173

(Lv et al., 2024)

Kdm4A[+]/Kdm4A^KO is a non-enhancer of visible | adult stage phenotype of Scer\GAL4^ey.200.Exel, TAF1B^RNAi.UAS

(Shalaby et al., 2017)

Kdm4A[+]/Kdm4A^KO is a non-enhancer of visible | adult stage phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

Kdm4A[+]/Kdm4A^KO is a non-enhancer of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

NOT Suppressor of

Statement

Reference

Kdm4A[+]/Kdm4A^KO is a non-suppressor of abnormal circadian rhythm | dominant | adult stage phenotype of CoRest^MI08173

(Lv et al., 2024)

Kdm4A[+]/Kdm4A^KO is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^ey.200.Exel, TAF1B^RNAi.UAS

(Shalaby et al., 2017)

Kdm4A[+]/Kdm4A^KO is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

Kdm4A[+]/Kdm4A^KO is a non-suppressor of abnormal size | adult stage phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

Other

Statement

Reference

Kdm4A^KO, Kdm4B^HA has partially lethal - majority die phenotype

(Shalaby et al., 2017)

Phenotype Manifest In

Enhancer of

Statement

Reference

Kdm4A[+]/Kdm4A^KO is an enhancer of wing vein phenotype of Scer\GAL4^C-765, smo^{5A.UAS.Tag:FLAG}

(Shalaby et al., 2017)

NOT Enhancer of

Statement

Reference

Kdm4A[+]/Kdm4A^KO is a non-enhancer of eye phenotype of Scer\GAL4^ey.200.Exel, TAF1B^RNAi.UAS

(Shalaby et al., 2017)

Kdm4A[+]/Kdm4A^KO is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

NOT Suppressor of

Statement

Reference

Kdm4A[+]/Kdm4A^KO is a non-suppressor of eye phenotype of Scer\GAL4^ey.200.Exel, TAF1B^RNAi.UAS

(Shalaby et al., 2017)

Kdm4A[+]/Kdm4A^KO is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, yki^{S168A.UAS.EGFP,Tag:HA}

(Shalaby et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Only about 20% of Kdm4A^KO,Kdm4B^KO double mutants survive to adulthood, while Kdm4A^KO;JHDM2^KO double mutants are viable and fertile.

Combination with a single copy of Kdm4A^KO does not modify the frequency of eye tissue to antenna-like malformed structures transformation in adult flies expressing TAF1B^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.200.Exel nor the massive eye overgrowth in flies expressing yki^{S168A.Scer\UAS.T:Avic\GFP-EGFP,T:Ivir\HA1} with Scer\GAL4^GMR.PF but it exacerbates the disrupted wing vein phenotype of flies expressing smo^{5A.Scer\UAS.T:Zzzz\FLAG} driven by Scer\GAL4^C-765.

(Shalaby et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

76241

w^*; TI{TI}Kdm4A^KO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

KDM4A^KO

(Lv et al., 2024, Shalaby et al., 2018, Pinzón et al., 2017, Shalaby et al., 2017)

Kdm4A^KO

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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