FlyBase Allele Report: Sindbis\rep[UAS.GFP]

General Information

Symbol

Sindbis\rep^UAS.GFP

Species

A. Sindbis virus

Name

FlyBase ID

FBal0321275

Feature type

allele

Associated gene

Sindbis\rep

Associated Insertion(s)

Carried in Construct

P{UAS-SINrep.GFP}

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Avadhanula et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-SINrep.GFP}

Cytology

Description

UASt regulates expression of the Sindbis (SIN) virus replicon RNA (encoding the 5' cap, the non-structural proteins, and the subgenomic promoter), which itself is capable of GFP expression from the subgenomic mRNA.

(Avadhanula et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Sindbis\rep

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Scer\GAL4^Act5C.PI-mediated expression of Zzzz\rep^{Scer\UAS.T:Avic\GFP} results in replication of the encoded Sindbis virus genome.

(Avadhanula et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Sindbis\rep^UAS.GFP has phenotype, enhanceable by Dcr-2[+]/Dcr-2^L811fsX

(Avadhanula et al., 2009)

Sindbis\rep^UAS.GFP has phenotype, enhanceable by Rel^E20/Rel[+]

(Avadhanula et al., 2009)

Sindbis\rep^UAS.GFP has phenotype, enhanceable by ird5[+]/IKKβ^EY02434

(Avadhanula et al., 2009)

Sindbis\rep^UAS.GFP has phenotype, enhanceable by imd^EY08573/imd[+]

(Avadhanula et al., 2009)

Sindbis\rep^UAS.GFP has phenotype, enhanceable by Fadd[+]/Fadd^EY10870

(Avadhanula et al., 2009)

Sindbis\rep^UAS.GFP has phenotype, enhanceable by Dredd[+]/Dredd^EY08404

(Avadhanula et al., 2009)

Sindbis\rep^UAS.GFP has phenotype, enhanceable by key[+]/key^c02831

(Avadhanula et al., 2009)

Sindbis\rep^UAS.GFP has phenotype, enhanceable by Stat92E^HJ/Stat92E[+]

(Avadhanula et al., 2009)

Sindbis\rep^UAS.GFP has phenotype, enhanceable by Tab2[+]/Tab2^201Y

(Avadhanula et al., 2009)

NOT Enhanced by

Statement

Reference

Sindbis\rep^UAS.GFP has phenotype, non-enhanceable by dl[+]/dl^KG06652

(Avadhanula et al., 2009)

Sindbis\rep^UAS.GFP has phenotype, non-enhanceable by Df(2L)Exel8036/+

(Avadhanula et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Heterozygosity for Dcr-2^L811fsX or Rel^E20 or Stat92E^HJ in a Scer\GAL4^Act5C.PI Zzzz\rep^{Scer\UAS.T:Avic\GFP} background results in increased Sindbis viral replication.

Heterozygosity for dl^KG06652 or Df(2L)Exel8036 (that uncovers Dif) in a Scer\GAL4^Act5C.PI Zzzz\rep^{Scer\UAS.T:Avic\GFP} background does not result in increased Sindbis viral replication.

Heterozygosity for imd^EY08573 or Fadd^EY10870 or Dredd^EY08404 or Tab2^201Y or ird5^EY02434 or key^c02831 in a Scer\GAL4^Act5C.PI Zzzz\rep^{Scer\UAS.T:Avic\GFP} background results in increased Sindbis viral replication.

(Avadhanula et al., 2009)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Sindbis\rep^{Scer\UAS.T:Avic\GFP}

Sindbis\rep^UAS.GFP

Zzzz\rep^{Scer\UAS.T:Avic\GFP}

Name Synonyms

Secondary FlyBase IDs

FBal0320661
FBal0285915

References (3)

Report Sections

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