FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Toll-6TIR-dead.UAS.Tag:FLAG,Tag:HA,Tag:polyHis
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General Information
Symbol
Dmel\Toll-6TIR-dead.UAS.Tag:FLAG,Tag:HA,Tag:polyHis
Species
D. melanogaster
Name
FlyBase ID
FBal0316488
Feature type
allele
Associated gene
Dmel\Toll-6
Associated Insertion(s)
Carried in Construct
P{UAS-Toll-6.TIR-dead}
Key Links
Transgenic product class
missense_variant
(Ward et al., 2015)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Ward et al., 2015)
Mutagen
in vitro construct
(Ward et al., 2015)
Progenitor genotype
Carried in construct
P{UAS-Toll-6.TIR-dead}
Cytology
Description

UAS regulatory sequences drive expression of Toll-6 in which two conserved residues critical for the function of the TIR domain have been mutated (mutations are E1136R in the BB loop and R1148E in the αA helix). The protein is tagged at the C-terminal end with a tag cassette composed of Tag:FLAG, 3xTag:HA, and 10xHIS.

(Ward et al., 2015)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Toll-6
Tagged with
Tag:FLAG
Tag:HA
Tag:polyHis
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Toll-6TIR-dead.UAS.Tag:FLAG,Tag:HA,Tag:polyHis/Scer\GAL4Mz19 rescues Toll-6ex13

      (Ward et al., 2015)
      Comments

      Expression of Toll-6TIR-dead.Scer\UAS.T:Zzzz\FLAG,T:Ivir\HA1,T:Zzzz\His6 under the control of Scer\GAL4Mz19 rescues the mistargeting phenotypes seen in the Scer\GAL4Mz19-positive projection neuron dendrites and VA1d olfactory receptor axons of Toll-6ex13 animals.

      (Ward et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Toll-6TIR-dead.Scer\UAS.T:Zzzz\FLAG,T:Ivir\HA1,T:Zzzz\His6
      Toll-6TIR-dead.UAS.Tag:FLAG,Tag:HA,Tag:polyHis
      Name Synonyms
      Secondary FlyBase IDs
        References (1)