FlyBase Allele Report: EBV\BRLF1[GMR.PA]

General Information

Symbol

EBV\BRLF1^GMR.PA

Species

L. Cercopithecine herpesvirus 15

Name

glass multimer reporter construct of Adamson

FlyBase ID

FBal0297399

Feature type

allele

Associated gene

EBV\BRLF1

Associated Insertion(s)

Carried in Construct

P{GMR-BRLF1.A}

Also Known As

GMR-R

Transgenic product class

cDNA

(Adamson and Lajeunesse, 2012)

wild_type

(Adamson and Lajeunesse, 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Adamson and Lajeunesse, 2012)

wild_type

(Adamson and Lajeunesse, 2012)

Mutagen

in vitro construct

(Adamson and Lajeunesse, 2012)

Progenitor genotype

Carried in construct

P{GMR-BRLF1.A}

Cytology

Description

GMR regulatory sequences drive expression of a EBV\BRLF1 cDNA.

(Adamson and Lajeunesse, 2012)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

EBV\BRLF1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of viral infectious disease

CEA

(Adamson and Lajeunesse, 2012)

CEC

(Adamson et al., 2014)

model of infectious mononucleosis

CEA

(Adamson and Lajeunesse, 2012)

CEC

(Adamson et al., 2014)

model of Epstein-Barr virus hepatitis

CEA

(Adamson and Lajeunesse, 2012)

CEC

(Adamson et al., 2014)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of viral infectious disease

is exacerbated by TBPH^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by chic^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by Shrm^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by RhoGEF2^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by CG16896^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by bin3^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by Pen^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by CdGAPr^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by gek^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by AP-2α^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by NAT1^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by Acaa^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by RN-tre^unspecified

(Adamson and Lajeunesse, 2012)

is ameliorated by Rack1^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by Fs(2)Ket^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by Chi^unspecified

(Adamson and Lajeunesse, 2012)

is ameliorated by dve^unspecified

(Adamson and Lajeunesse, 2012)

is ameliorated by Pax^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by Cdc14^unspecified

(Adamson and Lajeunesse, 2012)

is ameliorated by RanBPM^unspecified

(Adamson and Lajeunesse, 2012)

is ameliorated by dap^GMR.PU

(Adamson and Lajeunesse, 2012)

is ameliorated by scrib^j7B3

(Adamson and Lajeunesse, 2012)

is ameliorated by Rbf^GMR.PU

(Adamson and Lajeunesse, 2012)

is ameliorated by βCOP^unspecified

(Adamson and Lajeunesse, 2012)

is ameliorated by chico^KG00032

(Adamson and Lajeunesse, 2012)

is ameliorated by awd^j2A4

(Adamson and Lajeunesse, 2012)

is ameliorated by mTor^ΔP

(Adamson et al., 2014, Adamson and Lajeunesse, 2012)

is ameliorated by mTor^k17004

(Adamson et al., 2014, Adamson and Lajeunesse, 2012)

is ameliorated by rpr^GMR.PH

(Adamson and Lajeunesse, 2012)

is exacerbated by Rab5^EY10619

(Adamson and Lajeunesse, 2012)

is ameliorated by p53^GMR.Ex

(Adamson and Lajeunesse, 2012)

is exacerbated by Mer⁴

(Adamson and Lajeunesse, 2012)

is exacerbated by InR^E19

(Adamson and Lajeunesse, 2012)

is exacerbated by E2f1^GMR.PU

(Adamson and Lajeunesse, 2012)

is exacerbated by Csk^c04256

(Adamson and Lajeunesse, 2012)

is exacerbated by brat¹

(Adamson and Lajeunesse, 2012)

is exacerbated by brat^k06028

(Adamson and Lajeunesse, 2012)

is exacerbated by 14-3-3ζ^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by 14-3-3ζ^P1375

(Adamson and Lajeunesse, 2012)

model of infectious mononucleosis

is exacerbated by TBPH^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by Shrm^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by chic^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by RhoGEF2^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by CG16896^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by bin3^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by Pen^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by CdGAPr^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by AP-2α^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by gek^unspecified

(Adamson and Lajeunesse, 2012)

is ameliorated by RanBPM^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by Acaa^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by RN-tre^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by NAT1^unspecified

(Adamson and Lajeunesse, 2012)

is ameliorated by Rack1^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by Fs(2)Ket^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by Chi^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by Cdc14^unspecified

(Adamson and Lajeunesse, 2012)

is ameliorated by Pax^unspecified

(Adamson and Lajeunesse, 2012)

is ameliorated by dve^unspecified

(Adamson and Lajeunesse, 2012)

is ameliorated by scrib^j7B3

(Adamson and Lajeunesse, 2012)

is ameliorated by dap^GMR.PU

(Adamson and Lajeunesse, 2012)

is ameliorated by Rbf^GMR.PU

(Adamson and Lajeunesse, 2012)

is ameliorated by βCOP^unspecified

(Adamson and Lajeunesse, 2012)

is ameliorated by awd^j2A4

(Adamson and Lajeunesse, 2012)

is ameliorated by chico^KG00032

(Adamson and Lajeunesse, 2012)

is ameliorated by mTor^ΔP

(Adamson et al., 2014, Adamson and Lajeunesse, 2012)

is ameliorated by mTor^k17004

(Adamson et al., 2014, Adamson and Lajeunesse, 2012)

is ameliorated by rpr^GMR.PH

(Adamson and Lajeunesse, 2012)

is ameliorated by p53^GMR.Ex

(Adamson and Lajeunesse, 2012)

is exacerbated by Rab5^EY10619

(Adamson and Lajeunesse, 2012)

is exacerbated by Mer⁴

(Adamson and Lajeunesse, 2012)

is exacerbated by InR^E19

(Adamson and Lajeunesse, 2012)

is exacerbated by brat¹

(Adamson and Lajeunesse, 2012)

is exacerbated by Csk^c04256

(Adamson and Lajeunesse, 2012)

is exacerbated by E2f1^GMR.PU

(Adamson and Lajeunesse, 2012)

is exacerbated by brat^k06028

(Adamson and Lajeunesse, 2012)

is exacerbated by 14-3-3ζ^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by 14-3-3ζ^P1375

(Adamson and Lajeunesse, 2012)

model of Epstein-Barr virus hepatitis

is exacerbated by gek^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by TBPH^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by Shrm^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by chic^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by RhoGEF2^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by CdGAPr^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by CG16896^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by bin3^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by Pen^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by AP-2α^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by Acaa^unspecified

(Adamson and Lajeunesse, 2012)

is ameliorated by RanBPM^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by NAT1^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by RN-tre^unspecified

(Adamson and Lajeunesse, 2012)

is ameliorated by Pax^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by Fs(2)Ket^unspecified

(Adamson and Lajeunesse, 2012)

is ameliorated by Rack1^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by Chi^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by Cdc14^unspecified

(Adamson and Lajeunesse, 2012)

is ameliorated by dve^unspecified

(Adamson and Lajeunesse, 2012)

is ameliorated by βCOP^unspecified

(Adamson and Lajeunesse, 2012)

is ameliorated by scrib^j7B3

(Adamson and Lajeunesse, 2012)

is ameliorated by Rbf^GMR.PU

(Adamson and Lajeunesse, 2012)

is ameliorated by dap^GMR.PU

(Adamson and Lajeunesse, 2012)

is ameliorated by chico^KG00032

(Adamson and Lajeunesse, 2012)

is ameliorated by awd^j2A4

(Adamson and Lajeunesse, 2012)

is ameliorated by mTor^k17004

(Adamson et al., 2014, Adamson and Lajeunesse, 2012)

is ameliorated by mTor^ΔP

(Adamson et al., 2014, Adamson and Lajeunesse, 2012)

is ameliorated by rpr^GMR.PH

(Adamson and Lajeunesse, 2012)

is ameliorated by p53^GMR.Ex

(Adamson and Lajeunesse, 2012)

is exacerbated by Rab5^EY10619

(Adamson and Lajeunesse, 2012)

is exacerbated by Mer⁴

(Adamson and Lajeunesse, 2012)

is exacerbated by InR^E19

(Adamson and Lajeunesse, 2012)

is exacerbated by Csk^c04256

(Adamson and Lajeunesse, 2012)

is exacerbated by E2f1^GMR.PU

(Adamson and Lajeunesse, 2012)

is exacerbated by brat¹

(Adamson and Lajeunesse, 2012)

is exacerbated by brat^k06028

(Adamson and Lajeunesse, 2012)

is exacerbated by 14-3-3ζ^unspecified

(Adamson and Lajeunesse, 2012)

is exacerbated by 14-3-3ζ^P1375

(Adamson and Lajeunesse, 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

hyperplasia

(Adamson and Lajeunesse, 2012)

increased occurrence of cell division | larval stage

(Adamson and Lajeunesse, 2012)

visible

(Adamson and Lajeunesse, 2012)

Phenotype Manifest In

eye

(Adamson et al., 2014, Adamson and Lajeunesse, 2012)

eye disc | third instar larval stage

(Adamson and Lajeunesse, 2012)

eye disc morphogenetic furrow

(Adamson and Lajeunesse, 2012)

eye photoreceptor cell | increased number

(Adamson and Lajeunesse, 2012)

interommatidial bristle

(Adamson et al., 2014, Adamson and Lajeunesse, 2012)

ommatidium

(Adamson et al., 2014, Adamson and Lajeunesse, 2012)

Detailed Description

Statement

Reference

Flies expressing EBV\BRLF1^GMR.PA display rough eyes and extra small bristles.

(Adamson et al., 2014)

Expression of EBV\BRLF1^GMR.PA results in a rough adult eye phenotype. While wild-type eyes have an organized pattern of ommatidia, the flies expressing EBV\BRLF1^GMR.PA show unorganized ommatidial and bristle structure. The eye-phenotype is dose sensitive: the higher the dose, the more severe the phenotype is.

In EBV\BRLF1^GMR.PA eye discs, more mitotic cells are found in the morphogenetic furrow as well as more mitotic cells posterior to the furrow. EBV\BRLF1^GMR.PA discs have on average 1.5 times more cells in mitosis than wild-type discs. Overproliferation is also evident in EBV\BRLF1^GMR.PA adult eyes. Some ommatidia contain an extra photoreceptor cell. The eyes of the mutants also show an overabundance of pigment granules, which contributes to the darker adult eye color is these flies.

(Adamson and Lajeunesse, 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

EBV\BRLF1^GMR.PA has increased occurrence of cell division | larval stage phenotype, suppressible by EBV\BZLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

Other

Statement

Reference

EBV\BRLF1^GMR.PA, EBV\BZLF1^GMR.PA has visible phenotype

(Adamson and Lajeunesse, 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by 14-3-3ζ^P1375

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by brat¹

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by NAT1^unspecified

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by RN-tre^unspecified

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by 14-3-3ζ^unspecified

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by AP-2α^unspecified

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by bin3^unspecified

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by CdGAPr^unspecified

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by CG16896^unspecified

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by chic^unspecified

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by gek^unspecified

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by Pen^unspecified

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by brat^k06028

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by RhoGEF2^unspecified

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by Shrm^unspecified

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by TBPH^unspecified

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by Acaa^unspecified

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by Cdc14^unspecified

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by Csk^c04256

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by E2f1^GMR.PU

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by InR^E19

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by Mer⁴

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by Rab5^EY10619

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by Chi^unspecified

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, enhanceable by Fs(2)Ket^unspecified

(Adamson and Lajeunesse, 2012)

Suppressed by

Statement

Reference

EBV\BRLF1^GMR.PA has eye phenotype, suppressible | partially by Tor[+]/mTor^ΔP

(Adamson et al., 2014)

EBV\BRLF1^GMR.PA has interommatidial bristle phenotype, suppressible | partially by Tor[+]/mTor^ΔP

(Adamson et al., 2014)

EBV\BRLF1^GMR.PA has ommatidium phenotype, suppressible | partially by Tor[+]/mTor^ΔP

(Adamson et al., 2014)

EBV\BRLF1^GMR.PA has eye phenotype, suppressible | partially by Tor[+]/mTor^k17004

(Adamson et al., 2014)

EBV\BRLF1^GMR.PA has interommatidial bristle phenotype, suppressible | partially by Tor[+]/mTor^k17004

(Adamson et al., 2014)

EBV\BRLF1^GMR.PA has ommatidium phenotype, suppressible | partially by Tor[+]/mTor^k17004

(Adamson et al., 2014)

EBV\BRLF1^GMR.PA has eye phenotype, suppressible by p53^GMR.Ex

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, suppressible by rpr^GMR.PH

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, suppressible by dve^unspecified

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, suppressible by Pax^unspecified

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, suppressible by Rack1^unspecified

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, suppressible by RanBPM^unspecified

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, suppressible by mTor^ΔP

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, suppressible by mTor^k17004

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, suppressible by awd^j2A4

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, suppressible by chico^KG00032

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, suppressible by dap^GMR.PU

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, suppressible by Rbf^GMR.PU

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, suppressible by scrib^j7B3

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA has eye phenotype, suppressible by βCOP^unspecified

(Adamson and Lajeunesse, 2012)

Other

Statement

Reference

EBV\BRLF1^GMR.PA, EBV\BZLF1^GMR.PA has eye phenotype

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA, EBV\BZLF1^GMR.PA has ommatidium phenotype

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA, EBV\BZLF1^GMR.PA has interommatidial bristle phenotype

(Adamson and Lajeunesse, 2012)

EBV\BRLF1^GMR.PA, EBV\BZLF1^GMR.PA has eye disc | third instar larval stage phenotype

(Adamson and Lajeunesse, 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

While EBV\BZLF1^GMR.PA expression alone does not significantly alter the number of mitotic cells in eye discs, expression of EBV\BZLF1^GMR.PA reverses the EBV\BRLF1^GMR.PA overproliferation, such that the number of mitotic cells is lower than in wild-type controls. The number of short hair-like bristles in the adult eye is also decreased. The eye color of the double-transgenic flies is very similar to that of single-transgenic EBV\BZLF1^GMR.PA flies (orange), suggesting that pigment cells do not over-proliferate as in EBV\BRLF1^GMR.PA-eyes. The double-transgenic flies show a complete loss of ommatidia, although the bristles remain.

(Adamson and Lajeunesse, 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

EBV\BRLF1^GMR.PA

Name Synonyms

glass multimer reporter construct of Adamson

Secondary FlyBase IDs

References (2)

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