FlyBase Allele Report: Dmel\Toll-6[C1020Y.UAS.Tag:FLAG]

General Information

Symbol

Dmel\Toll-6^{C1020Y.UAS.Tag:FLAG}

Species

D. melanogaster

Name

FlyBase ID

FBal0295831

Feature type

allele

Associated gene

Dmel\Toll-6

Associated Insertion(s)

Carried in Construct

P{UAS-Toll-6.CY}

Also Known As

UAS-Toll-6^CY

Key Links

Transgenic product class

missense_variant

(McIlroy et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(McIlroy et al., 2013)

Mutagen

in vitro construct

(McIlroy et al., 2013)

Progenitor genotype

Carried in construct

P{UAS-Toll-6.CY}

Cytology

Description

Amino acid replacement: C1020Y.

(McIlroy et al., 2013)

UAS regulatory sequences drive expression of mutated Toll-6 coding sequences.

(McIlroy et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Toll-6

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^elav-C155

(McIlroy et al., 2013)

decreased cell death, with Scer\GAL4^elav-C155

(McIlroy et al., 2013)

increased cell death | pupal stage, with Scer\GAL4^elav.PU

(Foldi et al., 2017)

increased cell number | third instar larval stage, with Scer\GAL4^elav.PU

(Foldi et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Toll-6^{C1020Y.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^elav.PU results in significantly increased number of eve-positive neurons in the ventral nerve cord (VNC) in third instar larvae but also induces increased level of cell death (higher number of Dcp-1-positive cells) in the pupal VNC, as compared to driver-only controls.

(Foldi et al., 2017)

The expression of Toll-6^{C1020Y.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^Toll-6-D42 leads to a significant increase in the number of active zones, but not in the axonal length, at the larval NMJ, as compared to controls.

(Ulian-Benitez et al., 2017)

Constitutive overexpression of Toll-6, through expression of Toll-6^{C1020Y.Scer\UAS.T:Zzzz\FLAG} in the neurons, under the control of Scer\GAL4^elav-C155, causes axon targeting defects. Neuronal apoptosis is rescued in these overexpression mutants.

(McIlroy et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^elav.PU, Toll-6^{C1020Y.UAS.Tag:FLAG} has increased cell death | pupal stage phenotype, enhanceable by Myd88^kra-56/Myd88^kra-56

(Foldi et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^elav.PU, Toll-6^{C1020Y.UAS.Tag:FLAG} has increased cell death | pupal stage phenotype, suppressible by wek^EX14/Df(2R)BSC279

(Foldi et al., 2017)

Suppressor of

Statement

Reference

Scer\GAL4^Toll-7/Toll-6^{C1020Y.UAS.Tag:FLAG} is a suppressor of some die during embryonic stage | heat sensitive phenotype of NT1⁴¹, Toll-6²⁶

(McIlroy et al., 2013)

Scer\GAL4^elav-C155/Toll-6^{C1020Y.UAS.Tag:FLAG} is a suppressor of partially lethal phenotype of spz²

(McIlroy et al., 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^elav.PU, Toll-6^{C1020Y.UAS.Tag:FLAG} has larval ventral nerve cord | pupal stage phenotype, enhanceable by Myd88^kra-56/Myd88^kra-56

(Foldi et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^elav.PU, Toll-6^{C1020Y.UAS.Tag:FLAG} has larval ventral nerve cord | pupal stage phenotype, suppressible by wek^EX14/Df(2R)BSC279

(Foldi et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The increased cell death (number of apoptotic Dcp-1-positive cells) in the ventral nerve cord of pupae expressing Toll-6^{C1020Y.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^elav.PU can be increased further by Myd88^kra-56 homozygosity and rescued by combination with wek^EX14/Df(2R)BSC279.

(Foldi et al., 2017)

Pan-neuronal expression of Toll-6^{C1020Y.Scer\UAS.T:Zzzz\FLAG}, under the control of Scer\GAL4^elav-C155, suppresses the semi-lethality seen in spz² mutants.

(McIlroy et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Toll-6^{C1020Y.Scer\UAS.T:Zzzz\FLAG}

Toll-6^{C1020Y.UAS.Tag:FLAG}

Toll-6^CY

(Ulian-Benitez et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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