FlyBase Allele Report: Dmel\Top2[35-1]

General Information

Symbol

Dmel\Top2^35-1

Species

D. melanogaster

Name

FlyBase ID

FBal0282333

Feature type

allele

Associated gene

Dmel\Top2

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Hohl et al., 2012)

Mutagen

ethyl methanesulfonate

(Hohl et al., 2012)

Progenitor genotype

Cytology

Description

Amino acid replacement: R197C.

(Hohl et al., 2012)

Amino acid replacement: C347S.

(Hohl et al., 2012)

Amino acid replacement: P465S.

(Hohl et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:19,451,124..19,451,124 [-]

Nucleotide change:

T19451124A

Amino acid change:

C347S | Top2-PA; C347S | Top2-PB

Reported amino acid change:

C347S

Comment:

The nucleotide position of the Cys to Ser mutation is ambiguous. It could be a TGC to AGC or TGC to TCC codon change. The codon change was not specified. The codon change has been annotated as TGC to AGC. One of three amino acid substitutions observed in mutant strain.

(Hohl et al., 2012)

point_mutation

2L:19,451,640..19,451,640 [-]

Nucleotide change:

C19451640T

Amino acid change:

R197C | Top2-PA; R197C | Top2-PB

Reported amino acid change:

R197C

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. One of three amino acid substitutions observed in mutant strain.

(Hohl et al., 2012)

point_mutation

2L:19,450,770..19,450,770 [-]

Nucleotide change:

C19450770T

Amino acid change:

P465S | Top2-PA; P465S | Top2-PB

Reported amino acid change:

P465S

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. One of three amino acid substitutions observed in mutant strain.

(Hohl et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile (with Top2^17-1)

(Hohl et al., 2012)

female sterile (with Top2^17-3)

(Hohl et al., 2012)

female sterile (with Top2^17-6)

(Hohl et al., 2012)

female sterile (with Top2^35-5)

(Hohl et al., 2012)

female sterile (with Top2^35-13)

(Hohl et al., 2012)

lethal (with Df(2L)Exel9043)

(Hohl et al., 2012)

lethal | recessive

(Hohl et al., 2012)

male fertile (with Top2^17-1)

(Hohl et al., 2012)

male fertile (with Top2^17-6)

(Hohl et al., 2012)

male fertile (with Top2^35-5)

(Hohl et al., 2012)

male fertile (with Top2^35-13)

(Hohl et al., 2012)

male sterile (with Top2^17-3)

(Hohl et al., 2012)

partially lethal - majority die (with Top2^17-1)

(Hohl et al., 2012)

partially lethal - majority die (with Top2^17-3)

(Hohl et al., 2012)

partially lethal - majority die (with Top2^17-6)

(Hohl et al., 2012)

partially lethal - majority die (with Top2^35-5)

(Hohl et al., 2012)

partially lethal - majority die (with Top2^35-13)

(Hohl et al., 2012)

Phenotype Manifest In

female germline stem cell (with Top2^17-6)

(Hohl et al., 2012)

ovary (with Top2^17-6)

(Hohl et al., 2012)

Detailed Description

Statement

Reference

82% of Top2^35-1/Df(2L)Exel9043 animals survive to pupation.

Surviving Top2^17-6/Top2^35-1 females are sterile and lay very few eggs. The vast majority of the eggs laid no not undergo nuclear divisions. Strings of developing egg chambers are seen in the mutant females, but the ovaries are smaller than normal, disorganised and show evidence of egg chamber apoptosis. Complex defects are seen in the germaria, with both loss and gain of germline stem cells being seen.

(Hohl et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

Top2^35-13

(Hohl et al., 2012)

Top2^35-5

(Hohl et al., 2012)

Top2^17-6

(Hohl et al., 2012)

Top2^17-3

(Hohl et al., 2012)

Top2^17-1

(Hohl et al., 2012)

Comments

Top2^35-1 shows interallelic complementation with a number of other Top2 alleles. When Top2^35-1 is supplied paternally, the viability in combination with each allele as follows (% adult viability of progeny is shown in parentheses): Top2^17-1 (46%), Top2^17-3 (31%), Top2^17-6 (71%), Top2^35-5 (78%), Top2^35-13 (85%). When Top2^35-1 is supplied maternally, the viability in combination with each allele as follows (% adult viability of progeny is shown in parentheses): Top2^17-1 (56%), Top2^17-3 (22%), Top2^17-6 (58%), Top2^35-5 (86%), Top2^35-13 (79%).

(Hohl et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

35-1

(Hohl et al., 2012)

Top2^35-1

Top2^35-1-1

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

Open Close