FlyBase Allele Report: Dmel\Atg9[HMS01246]

General Information

Symbol

Dmel\Atg9^HMS01246

Species

D. melanogaster

Name

FlyBase ID

FBal0263259

Feature type

allele

Associated gene

Dmel\Atg9

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01246}

Also Known As

UAS-Atg9^RNAi, UAS-Atg9 RNAi, Atg9 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.HMS01246}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01246

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates carcinoma

modeled by ZnT86D^KK101698

(Wei et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell shape, with Scer\GAL4^NP0001

(Wen et al., 2017)

abnormal cell shape | adult stage, with Scer\GAL4^Tub.PU

(Wen et al., 2017)

increased cell size, with Scer\GAL4^NP0001

(Wen et al., 2017)

increased cell size | adult stage, with Scer\GAL4^Tub.PU

(Wen et al., 2017)

increased occurrence of cell division | adult stage, with Scer\GAL4^esg.PU

(Zhang et al., 2019)

short lived, with Scer\GAL4^Tub.PU

(Wen et al., 2017)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adulthood-only expression of Atg9^HMS01246 under the control of Scer\GAL4^esg.PU leads to a significant increase in the number of mitotic cells (phospho-H3 staining) in the adult midgut, as compared to controls. (The temporal regulation of expression is dependent on Gal80[ts] and induced by temperature shift to 29[o]C for 5-6 days during adulthood).

(Zhang et al., 2019)

The expression of Atg9^HMS01246 under the control of Scer\GAL4^Cg.PA blocks the starvation-induced accumulation of acidic vesicles (LysoTracker-positive) in the early third instar larval fat body, as compared to controls. Under standard conditions, there is no change in the number of autophagosome-like structures (Atg8a-positive), as compared to controls.

(Liu et al., 2018)

Constitutive expression of Atg9^HMS01246 under the control of Scer\GAL4^{Myo31DF-NP0001}, or adulthood-only expression under the combined control of either Scer\GAL4^tub.PU and Gal80[ts] or Scer\GAL4^{Myo31DF-NP0001} and Gal80[ts], leads to pronounced defects in the midgut, namely a severe increase in cell size and abnormal cell shape, as compared to controls. Adulthood-only expression under the combined control of Scer\GAL4^tub.PU and Gal80[ts] leads to intestinal barrier disfunction (as shown by the abnormal permeability to a non-absorbable food dye), leads to the failure to induce autophagy (assessed by the lysosome and autophagosome marker, Lysotracker) in the adult fat body in response to starvation, and leads to a decreased lifespan, as compared to controls.

Constitutive expression under the control of Scer\GAL4^Dl-05151-G or Scer\GAL4^Su(H).GBE, or adulthood-only expression under the combined control of Scer\GAL4^how-24B and Gal80[ts], has no obvious effect in the midgut, as compared to controls.

Clonal expression of Atg9^HMS01246 under the control of Scer\GAL4^Act.PU has no significant effect on the cell size within larval wing disc or larval eye disc clones, as compared to controls.

(Wen et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Atg9^HMS01246, Scer\GAL4^NP0001 has increased cell size phenotype, enhanceable by Akt^UAS.Exel, Scer\GAL4^NP0001

(Wen et al., 2017)

NOT Enhanced by

Statement

Reference

Atg9^HMS01246, Scer\GAL4^NP0001 has increased cell size phenotype, non-enhanceable by Patj^KK110089, Scer\GAL4^NP0001

(Wen et al., 2017)

Suppressed by

Statement

Reference

Atg9^HMS01246, Scer\GAL4^NP0001 has increased cell size phenotype, suppressible by Atg1^UAS.cSa, Scer\GAL4^NP0001

(Wen et al., 2017)

Atg9^HMS01246, Scer\GAL4^NP0001 has increased cell size phenotype, suppressible by S6k^KQ.UAS, Scer\GAL4^NP0001

(Wen et al., 2017)

Atg9^HMS01246, Scer\GAL4^NP0001 has increased cell size phenotype, suppressible by mTor^TED.UAS, Scer\GAL4^NP0001

(Wen et al., 2017)

Atg9^HMS01246, Scer\GAL4^NP0001 has increased cell size phenotype, suppressible by InR^HMS03166, Scer\GAL4^NP0001

(Wen et al., 2017)

Atg9^HMS01246, Scer\GAL4^NP0001 has increased cell size phenotype, suppressible by Pi3K92E^HMC05152, Scer\GAL4^NP0001

(Wen et al., 2017)

Atg9^HMS01246, Scer\GAL4^NP0001 has increased cell size phenotype, suppressible by Akt^HMS00007, Scer\GAL4^NP0001

(Wen et al., 2017)

Atg9^HMS01246, Scer\GAL4^NP0001 has increased cell size phenotype, suppressible by gig^UAS.cSa/Tsc1^UAS.cSa, Scer\GAL4^NP0001

(Wen et al., 2017)

Atg9^HMS01246, Scer\GAL4^NP0001 has increased cell size phenotype, suppressible by Patj^UAS.Tag:MYC, Scer\GAL4^NP0001

(Wen et al., 2017)

NOT suppressed by

Statement

Reference

Atg9^HMS01246, Scer\GAL4^NP0001 has increased cell size phenotype, non-suppressible by Rheb^UAS.cPa, Scer\GAL4^NP0001

(Wen et al., 2017)

Atg9^HMS01246, Scer\GAL4^NP0001 has increased cell size phenotype, non-suppressible by Tsc1^GD11836, Scer\GAL4^NP0001

(Wen et al., 2017)

Atg9^HMS01246, Scer\GAL4^NP0001 has increased cell size phenotype, non-suppressible by gig^GD1454, Scer\GAL4^NP0001

(Wen et al., 2017)

Atg9^HMS01246, Scer\GAL4^NP0001 has increased cell size phenotype, non-suppressible by Pi3K92E^CAAX.UAS, Scer\GAL4^NP0001

(Wen et al., 2017)

NOT Enhancer of

Statement

Reference

Atg9^HMS01246, Scer\GAL4^NP0001 is a non-enhancer of increased cell size phenotype of Patj^KK110089, Scer\GAL4^NP0001

(Wen et al., 2017)

Suppressor of

Statement

Reference

Atg9^HMS01246, Scer\GAL4^ey.PU is a suppressor of visible | adult stage phenotype of Scer\GAL4^ey.PU, ZnT86D^KK101698

(Wei et al., 2021)

NOT Suppressor of

Statement

Reference

Atg9^HMS01246, Scer\GAL4^GMR.long is a non-suppressor of visible | adult stage phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

Atg9^HMS01246, Scer\GAL4^Cg.PA is a non-suppressor of decreased cell size | third instar larval stage phenotype of Scer\GAL4^Cg.PA, Tom40^JF02030

(Liu et al., 2018)

Phenotype Manifest In

Enhanced by

Statement

Reference

Atg9^HMS01246, Scer\GAL4^NP0001 has adult midgut epithelium phenotype, enhanceable by Akt^UAS.Exel, Scer\GAL4^NP0001

(Wen et al., 2017)

NOT Enhanced by

Statement

Reference

Atg9^HMS01246, Scer\GAL4^NP0001 has adult midgut epithelium phenotype, non-enhanceable by Patj^KK110089, Scer\GAL4^NP0001

(Wen et al., 2017)

Suppressed by

Statement

Reference

Atg9^HMS01246, Scer\GAL4^NP0001 has adult midgut epithelium phenotype, suppressible by Atg1^UAS.cSa, Scer\GAL4^NP0001

(Wen et al., 2017)

Atg9^HMS01246, Scer\GAL4^NP0001 has adult midgut epithelium phenotype, suppressible by S6k^KQ.UAS, Scer\GAL4^NP0001

(Wen et al., 2017)

Atg9^HMS01246, Scer\GAL4^NP0001 has adult midgut epithelium phenotype, suppressible by mTor^TED.UAS, Scer\GAL4^NP0001

(Wen et al., 2017)

Atg9^HMS01246, Scer\GAL4^NP0001 has adult midgut epithelium phenotype, suppressible by InR^HMS03166, Scer\GAL4^NP0001

(Wen et al., 2017)

Atg9^HMS01246, Scer\GAL4^NP0001 has adult midgut epithelium phenotype, suppressible by Pi3K92E^HMC05152, Scer\GAL4^NP0001

(Wen et al., 2017)

Atg9^HMS01246, Scer\GAL4^NP0001 has adult midgut epithelium phenotype, suppressible by Akt^HMS00007, Scer\GAL4^NP0001

(Wen et al., 2017)

Atg9^HMS01246, Scer\GAL4^NP0001 has adult midgut epithelium phenotype, suppressible by gig^UAS.cSa/Tsc1^UAS.cSa, Scer\GAL4^NP0001

(Wen et al., 2017)

Atg9^HMS01246, Scer\GAL4^NP0001 has adult midgut epithelium phenotype, suppressible by Patj^UAS.Tag:MYC, Scer\GAL4^NP0001

(Wen et al., 2017)

NOT suppressed by

Statement

Reference

Atg9^HMS01246, Scer\GAL4^NP0001 has adult midgut epithelium phenotype, non-suppressible by Rheb^UAS.cPa, Scer\GAL4^NP0001

(Wen et al., 2017)

Atg9^HMS01246, Scer\GAL4^NP0001 has adult midgut epithelium phenotype, non-suppressible by Tsc1^GD11836, Scer\GAL4^NP0001

(Wen et al., 2017)

Atg9^HMS01246, Scer\GAL4^NP0001 has adult midgut epithelium phenotype, non-suppressible by gig^GD1454, Scer\GAL4^NP0001

(Wen et al., 2017)

Atg9^HMS01246, Scer\GAL4^NP0001 has adult midgut epithelium phenotype, non-suppressible by Pi3K92E^CAAX.UAS, Scer\GAL4^NP0001

(Wen et al., 2017)

NOT Enhancer of

Statement

Reference

Atg9^HMS01246, Scer\GAL4^NP0001 is a non-enhancer of adult midgut epithelium phenotype of Patj^KK110089, Scer\GAL4^NP0001

(Wen et al., 2017)

Suppressor of

Statement

Reference

Atg9^HMS01246, Scer\GAL4^ey.PU is a suppressor of eye phenotype of Scer\GAL4^ey.PU, ZnT86D^KK101698

(Wei et al., 2021)

Atg9^HMS01246, Scer\GAL4^ey.PU is a suppressor of eye disc | larval stage phenotype of Scer\GAL4^ey.PU, ZnT86D^KK101698

(Wei et al., 2021)

Atg9^HMS01246, Scer\GAL4^Cg.PA is a suppressor of autophagosome | early third instar larval stage phenotype of Scer\GAL4^Cg.PA, Tom40^JF02030

(Liu et al., 2018)

Atg9^HMS01246, Scer\GAL4^Cg.PA is a suppressor | partially of fat cell | early third instar larval stage phenotype of Scer\GAL4^Cg.PA, Tom40^JF02030

(Liu et al., 2018)

NOT Suppressor of

Statement

Reference

Atg9^HMS01246, Scer\GAL4^GMR.long is a non-suppressor of eye phenotype of Ire1^UAS.Tag:V5, Scer\GAL4^GMR.long

(Yan et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

The increased midgut cell size resulting from the expression of Atg9^HMS01246 under the control of Scer\GAL4^{Myo31DF-NP0001} is suppressed by the single co-expression of Atg1^Scer\UAS.cSa, S6k^KQ.Scer\UAS, Tor^TED.Scer\UAS, InR^HMS03166, Pi3K92E^HMC05152, Akt1^HMS00007 or Patj[Scer\UAS. T:Hsap \MYC], or by the combined co-expression of Tsc1^Scer\UAS.cSa and gig^Scer\UAS.cSa. Conversely, this phenotype is moderately enhanced by the co-expression of Akt1^{Scer\UAS.Exel}. However, this phenotype is not significantly affected by the co-expression of Rheb^Scer\UAS.cPa, Tsc1^GD11836, gig^GD1454 or Pi3K92E^{CAAX.Scer\UAS}.

The co-expression of Atg9^HMS01246 and Patj^KK110089 under the control of Scer\GAL4^{Myo31DF-NP0001} does not enhance the increased midgut cell size resulting from either single expression.

(Wen et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Atg9^HMS01246

(Billes et al., 2018, Nagy et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

Open Close