The expression of hop^HMS00761 under the control of Scer\GAL4^sd.PU leads to vestigial or absent wings and to the reciprocal formation of ectopic mesonotum structures, as compared to controls. Expression under the control of Scer\GAL4^en-e16E (and Dicer-2, for more efficient RNAi) leads to a severe size reduction of the third instar larval wing disc posterior compartment, as compared to controls.

The expression of hop^HMS00761 under the control of either Scer\GAL4^hh.PU or Scer\GAL4^ci.PU leads to a severe size reduction of both the mature leg disc and mature haltere disc posterior compartments, as compared to controls.

Expression of hop^HMS00761 under the control of Scer\GAL4^C587 results in germaria with fewer spectrosome-containing cells, as compared to controls.

Females heterozygous for hop^HMS00761 and Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} crossed to hop^HMS00761 males results in: 0% hatching; egg/embryonic phenotype: hop segmentation defects; maternal effect.