FlyBase Allele Report: Dmel\step[HMS00365]

General Information

Symbol

Dmel\step^HMS00365

Species

D. melanogaster

Name

FlyBase ID

FBal0248130

Feature type

allele

Associated gene

Dmel\step

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00365}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00365}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00365

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryo | embryonic cycle 11, with Scer\GAL4^{VP16.mat.αTub67C}

(Lee and Harris, 2013)

embryo | embryonic stage 5, with Scer\GAL4^{VP16.mat.αTub67C}

(Lee and Harris, 2013)

furrow canal, with Scer\GAL4^{VP16.mat.αTub67C}

(Lee and Harris, 2013)

Detailed Description

Statement

Reference

Embryos derived from mothers expressing step^HMS00365 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} have largely lost the endocytic tubules usually seen emanating from early cellularisation furrow canals. The furrow canal membrane cytoskeleton abnormally expands while remaining a tight sheet that displaces nuclei from the forming epithelium. The actomyosin expansion begins at the second peripheral division (cycle 11), increasing progressively through the remaining division cycles and into cellularisation.

(Lee and Harris, 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^{VP16.mat.αTub67C}, step^HMS00365 has embryo | embryonic stage 5 phenotype, suppressible by Rho1^72O/Rho1[+]

(Lee and Harris, 2013)

Scer\GAL4^{VP16.mat.αTub67C}, step^HMS00365 has furrow canal phenotype, suppressible by Rho1^72O/Rho1[+]

(Lee and Harris, 2013)

Scer\GAL4^{VP16.mat.αTub67C}, step^HMS00365 has embryo | embryonic stage 5 phenotype, suppressible by dia[+]/zip[+]/dia⁵/zip¹

(Lee and Harris, 2013)

Scer\GAL4^{VP16.mat.αTub67C}, step^HMS00365 has furrow canal phenotype, suppressible by dia[+]/zip[+]/dia⁵/zip¹

(Lee and Harris, 2013)

NOT suppressed by

Statement

Reference

Scer\GAL4^{VP16.mat.αTub67C}, step^HMS00365 has embryo | embryonic stage 5 phenotype, non-suppressible by dia[+]/dia⁵

(Lee and Harris, 2013)

Scer\GAL4^{VP16.mat.αTub67C}, step^HMS00365 has furrow canal phenotype, non-suppressible by dia[+]/dia⁵

(Lee and Harris, 2013)

Scer\GAL4^{VP16.mat.αTub67C}, step^HMS00365 has embryo | embryonic stage 5 phenotype, non-suppressible by zip[+]/zip¹

(Lee and Harris, 2013)

Scer\GAL4^{VP16.mat.αTub67C}, step^HMS00365 has furrow canal phenotype, non-suppressible by zip[+]/zip¹

(Lee and Harris, 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Maternal heterozygosity for Rho1^72O almost completely suppresses the furrow canal expansion phenotype seen in embryos derived from mothers expressing step^HMS00365 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}. The number of endocytic tubules emanating from furrow canals during early cellularisation is restored.

Maternal heterozygosity for dia⁵ does not suppress the furrow canal expansion phenotype seen in embryos derived from mothers expressing step^HMS00365 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}.

Maternal heterozygosity for zip¹ does not suppress the furrow canal expansion phenotype seen in embryos derived from mothers expressing step^HMS00365 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}.

Maternal heterozygosity for both dia⁵ and zip¹ suppresses the furrow canal expansion phenotype seen in embryos derived from mothers expressing step^HMS00365 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}.

(Lee and Harris, 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

step^HMS00365 is partially rescued by step^UASp.RR.EGFP/Scer\GAL4^{VP16.mat.αTub67C}

(Lee and Harris, 2013)

step^HMS00365 is partially rescued by Scer\GAL4^{VP16.mat.αTub67C}/step^{E173K.UASp.RR.EGFP}

(Lee and Harris, 2013)

Comments

Expression of step^{RNAi-resistant.E173K.Scer\UAS.P\T.T:Avic\GFP-EGFP} fails to rescue the furrow canal expansion and nuclear loss phenotypes seen when step^HMS00365 is expressed under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}.