FlyBase Allele Report: Scer\GAL4[rn-GAL4]

General Information

Symbol

Scer\GAL4^rn-GAL4

Species

S. cerevisiae

Name

FlyBase ID

FBal0247970

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

P{GawB}rn^GAL4

Carried in Construct

Also Known As

rn-GAL4, rotund-Gal4

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GawB}rn^GAL4

Cytology

Description

FlyBase curator comment: this entry is used in genotypes when it is known that the GAL4 driver used was an enhancer trap line generated in the targeted transposition screen described in FBrf0144823 (designed to replace the P{lArB} element in the P{lArB}rn⁸⁹ insertion with P{GawB} sequences), but where the particular GAL4 enhancer trap line used cannot be determined.

(FlyBase Curators, 2025-)

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

wing pouch

(Harris et al., 2016, Herrera et al., 2013)

early third instar larval stage

presumptive tarsal segment 2

(Sarno et al., 2011)

presumptive tarsal segment 3

(Sarno et al., 2011)

presumptive tarsal segment 4

(Sarno et al., 2011)

Additional Information

Statement

Reference

ScerGAL4^rn-GAL4 drives expression in the wing pouch throughout the third larval instar.

(Harris et al., 2016)

ScerGAL4^rn-GAL4 drives expression in the early, but not late, third instar leg discs in a ring corresponding to developing tarsal segments.

(Sarno et al., 2011)

Marker for

Reflects expression of

(Harris et al., 2016, Herrera et al., 2013, Sarno et al., 2011)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased size | adult stage | somatic clone, with egr^UAS.cMa

(Harris et al., 2020)

hyperplasia | third instar larval stage, with yki^{UAS.cOa.Tag:V5}

(Harris et al., 2016)

visible, with ss^UAS.cCa

(Céspedes et al., 2010)

visible | adult stage | somatic clone, with egr^UAS.cMa

(Harris et al., 2020)

visible | chemical conditional, with Mmus\Ahr^UAS.cCa

(Céspedes et al., 2010)

Phenotype Manifest In

leg, with ss^UAS.cCa

(Céspedes et al., 2010)

leg | chemical conditional, with Mmus\Ahr^UAS.cCa

(Céspedes et al., 2010)

tarsal segment, with ss^UAS.cCa

(Céspedes et al., 2010)

wing | somatic clone, with egr^UAS.cMa

(Harris et al., 2020)

wing blade, with jp^UAS

(Calpena et al., 2018)

wing disc | third instar larval stage, with yki^{UAS.cOa.Tag:V5}

(Harris et al., 2016)

wing vein, with jp^UAS

(Calpena et al., 2018)

Detailed Description

Statement

Reference

Wing pouch ablation by the expression of egr^UAS.cMa under the control of Scer\GAL4^rn-GAL4 during the larval stage (induced by heat-shock) results in nicked, notched and ablated wing blades.

(Harris et al., 2020)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^rn-GAL4, egr^UAS.cMa has decreased size | adult stage | somatic clone phenotype, enhanceable by Scer\GAL4^rn-GAL4/aspr^HMJ22471

(Harris et al., 2020)

Scer\GAL4^rn-GAL4, egr^UAS.cMa has decreased size | adult stage | somatic clone phenotype, enhanceable by Scer\GAL4^rn-GAL4/aspr^MI02471