At embryonic stage 11, EcollacZHsp23.1.8 transcript is expressed in MP2 neuroblasts, one laterally localized chordotonal organ precursor per hemisegment, and in the amnioserosa.By stage 13, EcollacZHsp23.1.8 transcript is expressed in one laterally localized chordotonal neuron per abdominal hemisegment, and one dorsally localized chordotonal neuron in the second and third thoracic segments. It is also expressed in the dMP2, vMP2 and VUM neurons.. Expression in midline glial cells begins during stage 13, and expression becomes restricted to midline glial cells by late embryonic stages.