FlyBase Allele Report: Dmel\shot[JF02971]

Expressing shot^JF02971 under the control of Scer\GAL4^{SPARC-MI00329-GAL4} results in third instar larval adipocytes presenting plasma membrane invaginations where aberrant extracellular matrix material accumulates (Vkg.GFP), despite of no obvious secretion defects (secr.GFP).

Expressing shot^JF02971 under the control of Scer\GAL4^Cg.PA results in third instar larval adipocytes presenting enlarged, deformed and highly eccentric nuclei, plasma membrane invaginations where aberrant extracellular matrix material accumulates (LanA.GFP, LanB1.GFP or Ndg.GFP). In agreement, Golgi units lose their homogeneous distribution throughout the cytoplasm and seem to gravitate toward a single focus near the nucleus. These adipocytes also exhibit a large juxtanuclear focus of actively growing microtubules, in contrast to a normal perinuclear distribution in controls. This ectopic microtubule focus concentrates actin filaments. The long tubular extensions are reminiscent of a mitotic cytokinesis furrow, although no mitosis (anti-Phospho-H3) can be seen. There is no effect on adipocyte ploidy.

Conditional expression of shot^JF02971 from the L2 under the control of Scer\GAL4^Cg.PA and Gal80[ts] also leads to aberrant matrix accumulations (vkg.GFP) and concentration of microtubules in a large juxtanuclear focus: 36h induction leads to an incipient microtubule focus abutting the perinuclear network; 48h or 60h induction causes more dramatic microtubule single point concentrations, similar to continuous expression.

Expressing shot^JF02971 under the control of Scer\GAL4^rn-GAL4-5, Scer\GAL4^pnr-MD237 and Scer\GAL4^byn.PU caused both aberrant microtubule foci formation and mispositioned nuclei in the salivary gland, larval epidermis, and rectal papilla, respectively. The epidermis and salivary gland juxtanuclear foci exhibit non-centrosomal minus-ends (Patronin.GFP).

Expressing shot^JF02971 under the control of Scer\GAL4^NP0001 leads to the concentration of microtubule minus-ens in a juxtanuclear focus in third instar larval midgut enteroblasts.

(Sun et al., 2019)

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Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

shot^JF02971, Scer\GAL4^ey.PH is an enhancer of visible | adult stage phenotype of Hsap\HOXA9::Hsap\NUP98^WT.UAS, Scer\GAL4^ey.PH

(Gavory et al., 2021)

shot^JF02971, Scer\GAL4^ey.PH is an enhancer of increased size | adult stage phenotype of Hsap\HOXA9::Hsap\NUP98^WT.UAS, Scer\GAL4^ey.PH

(Gavory et al., 2021)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Cg.PA, shot^JF02971 has fat cell | third instar larval stage phenotype, suppressible by CycE^JF02473, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^JF02971 has nucleus | third instar larval stage phenotype, suppressible by CycE^JF02473, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^JF02971 has fat cell | third instar larval stage phenotype, suppressible by Cdk2^HMS01543, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^JF02971 has nucleus | third instar larval stage phenotype, suppressible by Cdk2^HMS01543, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^JF02971 has fat cell | third instar larval stage phenotype, suppressible by E2f1^JF02718, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^JF02971 has nucleus | third instar larval stage phenotype, suppressible by E2f1^JF02718, Scer\GAL4^Cg.PA

(Sun et al., 2019)

NOT suppressed by

Statement

Reference

Scer\GAL4^Cg.PA, shot^JF02971 has fat cell | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Cg.PA/vkg^KK111668

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^JF02971 has plasma membrane | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Cg.PA/vkg^KK111668

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^JF02971 has fat cell | third instar larval stage phenotype, non-suppressible by γTub23C^A15-2/Df(2L)ED4651

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^JF02971 has microtubule | third instar larval stage phenotype, non-suppressible by γTub23C^A15-2/Df(2L)ED4651

(Sun et al., 2019)

Scer\GAL4^{SPARC-MI00329-GAL4}, shot^JF02971 has fat cell | third instar larval stage phenotype, non-suppressible by GM130^Δ23/GM130^Δ23

(Sun et al., 2019)

Scer\GAL4^{SPARC-MI00329-GAL4}, shot^JF02971 has microtubule | third instar larval stage phenotype, non-suppressible by GM130^Δ23/GM130^Δ23

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^JF02971 has fat cell | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Cg.PA/pav^GL01316

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^JF02971 has endoplasmic reticulum | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Cg.PA/pav^GL01316

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^JF02971 has fat cell | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Cg.PA/pbl^JF02979

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^JF02971 has endoplasmic reticulum | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Cg.PA/pbl^JF02979

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^JF02971 has fat cell | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Cg.PA/tum^HMS01417

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^JF02971 has endoplasmic reticulum | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Cg.PA/tum^HMS01417

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^JF02971 has fat cell | third instar larval stage phenotype, non-suppressible by γTub23C^JF01722, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Scer\GAL4^Cg.PA, shot^JF02971 has microtubule | third instar larval stage phenotype, non-suppressible by γTub23C^JF01722, Scer\GAL4^Cg.PA

(Sun et al., 2019)

Enhancer of

Statement

Reference

shot^JF02971, Scer\GAL4^ey.PH is an enhancer of eye phenotype of Hsap\HOXA9::Hsap\NUP98^WT.UAS, Scer\GAL4^ey.PH

(Gavory et al., 2021)

shot^JF02971, Scer\GAL4^ey.PH is an enhancer of adult cuticle phenotype of Hsap\HOXA9::Hsap\NUP98^WT.UAS, Scer\GAL4^ey.PH

(Gavory et al., 2021)

shot^JF02971, Scer\GAL4^ey.PH is an enhancer of adult head phenotype of Hsap\HOXA9::Hsap\NUP98^WT.UAS, Scer\GAL4^ey.PH

(Gavory et al., 2021)

Other

Statement

Reference

Cdk1^JF03004, Scer\GAL4^Cg.PA, shot^JF02971 has embryonic/larval hemocyte | third instar larval stage phenotype

(Sun et al., 2019)

Cdk1^JF03004, Scer\GAL4^Cg.PA, shot^JF02971 has microtubule organizing center | third instar larval stage phenotype

(Sun et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expressing shot^JF02971 and Cdk1^JF03004 under the control of Scer\GAL4^Cg.PA causes concentration of dynamic microtubules in a single juxtanuclear focus in hemocytes.

(Sun et al., 2019)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

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Mutant

Wild-type

Stocks (1)

Bloomington

28336

y¹ v¹; P{TRiP.JF02971}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

shot^JF02971

(Zheng et al., 2020)

shotⁱ

(Sun et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (9)

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