FlyBase Allele Report: Dmel\msn[KK108948]

General Information

Symbol

Dmel\msn^KK108948

Species

D. melanogaster

Name

FlyBase ID

FBal0235935

Feature type

allele

Associated gene

Dmel\msn

Associated Insertion(s)

Carried in Construct

P{KK108948}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK108948}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK108948

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^Cg.PU

(Havula et al., 2022)

abnormal starvation stress response | adult stage, with Scer\GAL4^Mef2.PU

(Havula et al., 2022)

abnormal starvation stress response | adult stage, with Scer\GAL4^NP0001

(Havula et al., 2022)

abnormal starvation stress response | adult stage | nutrition conditional, with Scer\GAL4^Cg.PU

(Havula et al., 2022)

chemical sensitive, with Scer\GAL4^elav-C155

(Ghezzi et al., 2013)

lethal - all die before end of P-stage, with Scer\GAL4^Tub.PU

(Havula et al., 2022)

long lived | :calorie restriction conditional | nutrition conditional, with Scer\GAL4^Cg.PU

(Havula et al., 2022)

majority die during larval stage | nutrition conditional, with Scer\GAL4^Tub.PU

(Havula et al., 2022)

partially lethal - majority die | nutrition conditional, with Scer\GAL4^Tub.PU

(Havula et al., 2022)

short lived | calorie restriction conditional, with Scer\GAL4^Mef2.PU

(Havula et al., 2022)

short lived | calorie restriction conditional, with Scer\GAL4^NP0001

(Havula et al., 2022)

some die during larval stage | nutrition conditional, with Scer\GAL4^Tub.PU

(Havula et al., 2022)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adults expressing msn^KK108948 under the control of Scer\GAL4^Cg.PU show decreased activity, as well as increased survival to starvation when reared under high sugar diet.

(Havula et al., 2022)

Expressing msn^KK108948 under the control of Scer\GAL4^C587 (together with Dicer-2, for a more efficient RNAi) does not induce any obvious L3 spermatogenesis defects.

(La Marca et al., 2019)

RNAi knockdown of msn, through expression of msn^KK108948 in the posterior compartment of the wing disc, under the control of either Scer\GAL4^en.PU or Scer\GAL4^hh.PU, does not affect levels of cell death.

(Wong et al., 2014)

Scer\GAL4^elav-C155>msn^KK108948 significantly reduces benzyl alcohol tolerance, but failed to affect ethanol tolerance.

(Ghezzi et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

msn^KK108948, Scer\GAL4^dpp.blk1 is a non-suppressor of visible phenotype of HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

Other

Statement

Reference

Scer\GAL4^hh.PU, mad2^GD16427, msn^KK108948 has increased cell death phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, mad2^GD16427, msn^KK108948 has increased cell death phenotype

(Wong et al., 2014)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

msn^KK108948, Scer\GAL4^dpp.blk1 is a non-suppressor of wing phenotype of HIV-1\Vpu^UAS.cLa, Scer\GAL4^dpp.blk1

(Marchal et al., 2012)

Other

Statement

Reference

Scer\GAL4^hh.PU, mad2^GD16427, msn^KK108948 has wing disc phenotype

(Wong et al., 2014)

Scer\GAL4^en.PU, mad2^GD16427, msn^KK108948 has wing disc phenotype

(Wong et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when msn^KK108948 is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU. There is also a concomitant increase in cell death in these double mutants. Consistent with high levels of cell death and consequent loss of tissue, adult wings show notching when JNK signalling is reduced in a chromosome instability background (through expression of mad2^GD16427).

(Wong et al., 2014)

Xenogenetic Interactions

Statement

Reference

Co-expression of msn^KK108948 does not suppress the wing defects caused by expression of HIV-1\Vpu^Scer\UAS.cLa under the control of Scer\GAL4^dpp.blk1.

(Marchal et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v101517

P{KK108948}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

msn^KK108948

(Plutoni et al., 2019, Ghezzi et al., 2013)

msn^RNAi

(Chen et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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