FlyBase Allele Report: Dmel\Marf[KK105681]

General Information

Symbol

Dmel\Marf^KK105681

Species

D. melanogaster

Name

FlyBase ID

FBal0233716

Feature type

allele

Associated gene

Dmel\Marf

Associated Insertion(s)

Carried in Construct

P{KK105681}

Also Known As

UAS-Marf RNAi, UAS-Marf^RNAi, dMfn RNAi, marf RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK105681}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK105681

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Charcot-Marie-Tooth disease type 2A2A

CEA

(Garrido-Maraver et al., 2019)

CEC

(Garrido-Maraver et al., 2019)

model of Parkinson's disease 6

CEA with Pink1^B9

(Valadas et al., 2018)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by park²⁵

(Celardo et al., 2016)

modeled by Pink1^B9

(Celardo et al., 2016)

model of Charcot-Marie-Tooth disease type 2A2A

is ameliorated by Nmdmc^UAS.Tag:HA

(Garrido-Maraver et al., 2019)

ameliorates Parkinson's disease 2

modeled by park^Δ21, park¹

(Valadas et al., 2018)

exacerbates frontotemporal dementia and/or amyotrophic lateral sclerosis 2

modeled by Chchd2^S81L.UAS

(Baek et al., 2021)

ameliorates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Campesan et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^VGlut1-OK371

(Smith et al., 2019)

decreased size, with Scer\GAL4^Mef2.PR

(Katti et al., 2021)

decreased size | adult stage, with Scer\GAL4^Mhc.PW

(Katti et al., 2021)

flightless, with Scer\GAL4^Mef2.PR

(Katti et al., 2021)

increased cell number | third instar larval stage, with Scer\GAL4^Antp-10

(Sinenko et al., 2011)

Phenotype Manifest In

axon | adult stage, with Scer\GAL4^VGlut1-OK371

(Smith et al., 2019)

indirect flight muscle cell | adult stage, with Scer\GAL4^Mef2.PR

(Katti et al., 2021)

indirect flight muscle cell | adult stage, with Scer\GAL4^Mhc.PW

(Katti et al., 2021)

indirect flight muscle cell | P-stage, with Scer\GAL4^Mef2.PR

(Katti et al., 2021)

lamellocyte | third instar larval stage, with Scer\GAL4^Antp-10

(Sinenko et al., 2011)

mitochondrion, with Scer\GAL4^Mhc.PW

(Imai et al., 2010)

mitochondrion | adult stage, with Scer\GAL4^VGlut1-OK371

(Smith et al., 2019)

mitochondrion | adult stage | increased number, with Scer\GAL4^Mef2.PR

(Katti et al., 2021)

mitochondrion | adult stage | increased number, with Scer\GAL4^Mhc.PW

(Katti et al., 2021)

mitochondrion | P-stage, with Scer\GAL4^Mef2.PR

(Katti et al., 2021)

sarcomere, with Scer\GAL4^Mef2.PR

(Katti et al., 2021)

skeletal muscle myofibril, with Scer\GAL4^Mef2.PR

(Katti et al., 2021)

Detailed Description

Statement

Reference

Expression of Marf^KK105681 under the control of Scer\GAL4^VGlut-OK371 significantly reduces mitochondrial length in axons of adult neurons, compared to controls, with no effect on mitochondrial number, compared to controls.

(Smith et al., 2019)

Expression of Marf^KK105681 in the posterior signalling center (PSC) under the control of either Scer\GAL4^Antp-10 leads to an increase in the number of circulating lamellocytes.

(Sinenko et al., 2011)

Mitochondria in the indirect flight muscles are shorter in the long-axis direction than normal in 5 day old adults expressing Marf^KK105681 under the control of Scer\GAL4^Mhc.PW.

(Imai et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Marf^KK105681, Scer\GAL4^VGlut1-OK371 has abnormal neuroanatomy | adult stage phenotype, non-enhanceable | somatic clone by gfzf⁵⁴¹

(Smith et al., 2019)

NOT suppressed by

Statement

Reference

Marf^KK105681, Scer\GAL4^VGlut1-OK371 has abnormal neuroanatomy | adult stage phenotype, non-suppressible | somatic clone by gfzf⁵⁴¹

(Smith et al., 2019)

Enhancer of

Statement

Reference

Marf^KK105681, Scer\GAL4^GMR.PU is an enhancer of visible | adult stage | progressive phenotype of Chchd2^S81L.UAS, Scer\GAL4^GMR.PU

(Baek et al., 2021)

NOT Enhancer of

Statement

Reference

Scer\GAL4^αTub84B.PL/Marf^KK105681 is a non-enhancer of lethal - all die during embryonic stage phenotype of gfzf⁵⁴¹

(Smith et al., 2019)

Suppressor of

Statement

Reference

Scer\GAL4^VGlut1-OK371/Marf^KK105681 is a suppressor | partially of abnormal neurophysiology | somatic clone | adult stage phenotype of gfzf⁵⁴¹

(Smith et al., 2019)

Scer\GAL4^VGlut1-OK371/Marf^KK105681 is a suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of gfzf⁵⁴¹

(Smith et al., 2019)

Scer\GAL4^elav.PU/Marf^KK105681 is a suppressor of abnormal neuroanatomy | adult stage phenotype of park²⁵

(Celardo et al., 2016)

Scer\GAL4^elav.PU/Marf^KK105681 is a suppressor | partially of abnormal neuroanatomy | adult stage phenotype of Pink1^B9

(Celardo et al., 2016)

NOT Suppressor of

Statement

Reference

Scer\GAL4^αTub84B.PL/Marf^KK105681 is a non-suppressor of lethal - all die during embryonic stage phenotype of gfzf⁵⁴¹

(Smith et al., 2019)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Marf^KK105681, Scer\GAL4^VGlut1-OK371 has mitochondrion | adult stage phenotype, non-enhanceable | somatic clone by gfzf⁵⁴¹

(Smith et al., 2019)

Marf^KK105681, Scer\GAL4^VGlut1-OK371 has axon | adult stage phenotype, non-enhanceable | somatic clone by gfzf⁵⁴¹

(Smith et al., 2019)

NOT suppressed by

Statement

Reference

Marf^KK105681, Scer\GAL4^VGlut1-OK371 has mitochondrion | adult stage phenotype, non-suppressible | somatic clone by gfzf⁵⁴¹

(Smith et al., 2019)

Marf^KK105681, Scer\GAL4^VGlut1-OK371 has axon | adult stage phenotype, non-suppressible | somatic clone by gfzf⁵⁴¹

(Smith et al., 2019)

Marf^KK105681, Scer\GAL4^Mhc.PW has mitochondrion phenotype, non-suppressible by Pgam5¹

(Imai et al., 2010)

Enhancer of

Statement

Reference

Marf^KK105681, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Chchd2^S81L.UAS, Scer\GAL4^GMR.PU

(Baek et al., 2021)

Suppressor of

Statement

Reference

Scer\GAL4^NP0001/Marf^KK105681 is a suppressor of gut | prepupal stage phenotype of Vps13D^MI11101/Vps13D^ΔUBA

(Shen et al., 2021)

Scer\GAL4^NP0001/Marf^KK105681 is a suppressor of mitochondrion | prepupal stage phenotype of Vps13D^MI11101/Vps13D^ΔUBA

(Shen et al., 2021)

Scer\GAL4^NP0001/Marf^KK105681 is a suppressor of endoplasmic reticulum | prepupal stage phenotype of Vps13D^MI11101/Vps13D^ΔUBA

(Shen et al., 2021)

Marf^KK105681, Scer\GAL4^NP0001 is a suppressor of gut | prepupal stage phenotype of Scer\GAL4^NP0001, Tango5^KK108366

(Shen et al., 2021)

Marf^KK105681, Scer\GAL4^NP0001 is a suppressor of mitochondrion | prepupal stage phenotype of Scer\GAL4^NP0001, Tango5^KK108366

(Shen et al., 2021)

Scer\GAL4^VGlut1-OK371/Marf^KK105681 is a suppressor | partially of retina | somatic clone | adult stage phenotype of gfzf⁵⁴¹

(Smith et al., 2019)

Scer\GAL4^VGlut1-OK371/Marf^KK105681 is a suppressor of mitochondrion | somatic clone | adult stage phenotype of gfzf⁵⁴¹

(Smith et al., 2019)

Scer\GAL4^VGlut1-OK371/Marf^KK105681 is a suppressor of axon | somatic clone | adult stage phenotype of gfzf⁵⁴¹

(Smith et al., 2019)

Scer\GAL4^da.PU/Marf^KK105681 is a suppressor | partially of adult thorax phenotype of Pink1^B9

(Celardo et al., 2016)

Scer\GAL4^elav.PU/Marf^KK105681 is a suppressor of adult brain phenotype of park²⁵

(Celardo et al., 2016)

Scer\GAL4^elav.PU/Marf^KK105681 is a suppressor of mitochondrion | adult stage phenotype of park²⁵

(Celardo et al., 2016)

Scer\GAL4^elav.PU/Marf^KK105681 is a suppressor of endoplasmic reticulum | adult stage phenotype of park²⁵

(Celardo et al., 2016)

Scer\GAL4^elav.PU/Marf^KK105681 is a suppressor of dopaminergic PPL1 neuron | adult stage phenotype of park²⁵

(Celardo et al., 2016)

Scer\GAL4^da.PU/Marf^KK105681 is a suppressor of adult thorax phenotype of park²⁵

(Celardo et al., 2016)

Scer\GAL4^elav.PU/Marf^KK105681 is a suppressor | partially of adult brain phenotype of Pink1^B9

(Celardo et al., 2016)

Scer\GAL4^elav.PU/Marf^KK105681 is a suppressor | partially of mitochondrion | adult stage phenotype of Pink1^B9

(Celardo et al., 2016)

Scer\GAL4^elav.PU/Marf^KK105681 is a suppressor | partially of endoplasmic reticulum | adult stage phenotype of Pink1^B9

(Celardo et al., 2016)

Scer\GAL4^elav.PU/Marf^KK105681 is a suppressor | partially of dopaminergic PPL1 neuron | adult stage phenotype of Pink1^B9

(Celardo et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of mitochondria-endoplasmic reticulum contacts observed in the brain of either Pink1^B9 or park²⁵/park²⁵ mutant adults is strongly suppressed by expression of Marf^KK105681 RNAi under the control of Scer\GAL4^elav.PU in the mutant background.

Expression of Marf^KK105681 under the control of Scer\GAL4^da.PU strongly suppresses the 'crushed thorax' phenotype characteristic for either Pink1^B9 or park²⁵/park²⁵ mutant adults.

(Celardo et al., 2016)

Pgam5¹ does not suppress the abnormal mitochondrial phenotype (mitochondria in the indirect flight muscles are shorter in the long-axis direction than normal) seen in 5 day old adults expressing Marf^KK105681 under the control of Scer\GAL4^Mhc.PW.

(Imai et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v105261

P{KK105681}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Marf^KK105681

marf^RNAi

(Bonnay et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

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