FlyBase Allele Report: Dmel\Rab5[S43N.UASp.YFP]

General Information

Symbol

Dmel\Rab5^{S43N.UASp.YFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0215393

Feature type

allele

Associated gene

Dmel\Rab5

Associated Insertion(s)

Carried in Construct

P{UASp-YFP.Rab5.S43N}

Also Known As

rab5^DN, UAS-Rab5DN, UAS-rab5^DN, DN-Rab5, UAS-Rab5-DN, UAS-YFP-rab5^DN, Rab5DN, UAS-YFP::Rab5^DN, UAS-YFPRab5^S43N, DN Rab5

Key Links

Transgenic product class

dominant_negative_variant

(Zhang et al., 2007)

missense_variant

(Zhang et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Zhang et al., 2007)

missense_variant

(Zhang et al., 2007)

Mutagen

in vitro construct

(Zhang et al., 2007, Zhang et al., 2007)

Progenitor genotype

Carried in construct

P{UASp-YFP.Rab5.S43N}

Cytology

Description

Amino acid replacement: S43N.

(Zhang et al., 2007)

UASp regulatory sequences drive expression of a predicted dominant negative form of Rab5 that is tagged at the N-terminal end with YFP.

(Zhang et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Rab5

Tagged with

YFP

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Alzheimer's disease

modeled by Hsap\APP^695.UAS

(Xu et al., 2016)

ameliorates motor neuron disease

modeled by scat^HMS01910

(Patel et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

1 day old adults expressing Rab5^{S43N.UASp.YFP} under the control of Scer\GAL4^futsch-C380 exhibit decreased climbing ability.

(Wilkinson et al., 2021)

Expression of Rab5^{S43N.UASp.YFP} under the control of CtxGlia-SplitGal4 (Hsim\VP16^AD.nrv2 and Scer\GAL4^{DBD.wrapper.932i}) leads to an altered cortex glial morphology in third instar larvae when compared to controls.

(Coutinho-Budd et al., 2017)

Expression of Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^Appl.G1a does not impair axonal transport in the segmental nerves in third instar larvae.

(Xu et al., 2016)

Expression of Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of either Scer\GAL4^eg-Mz360, Scer\GAL4^eve.RN2 or Scer\GAL4^elav-C155 does not result in any defects in axon outgrowth in the embryo.

(van den Brink et al., 2013)

Expression of Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^slbo.2.6 impairs border cell migration in the developing egg chamber. No border cell cluster dissociation is seen.

(Chen et al., 2012)

Expression of Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^GMR.PF during photoreceptor development results in defects in rhabdomere morphogenesis; sheets of apposed plasma membrane emanating from the rhabdomere are readily detected. This phenotype is light-independent.

(Pinal and Pichaud, 2011)

Expression of Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP} driven by Scer\GAL4^slbo.2.6 results in the impairment of border cell migration during egg chamber development.

(Assaker et al., 2010)

Expression of Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^btl.PS results in a tracheal lumen defect (convoluted and dilated dorsal trunks) in 24% of embryos.

(Hsouna et al., 2010)

Embryos expressing Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^Mef2.PR show muscle detachment defects. These defects are due to separation between the integrins at the end of the muscles and the extracellular matrix.

(Yuan et al., 2010)

Pupal eye somatic clones expressing Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP} do not affect apical cell area.

(Warner and Longmore, 2009)

Epithelial defects are seen in the egg chambers of females expressing Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^55B; 14% show flattening of epithelial cells and 9% show piling up or loose cells.

(Woolworth et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Rab5^{S43N.UASp.YFP}, Scer\GAL4^futsch-C380 has abnormal locomotor behavior | female | adult stage phenotype, suppressible by scat^HMS01910, Scer\GAL4^futsch-C380

(Wilkinson et al., 2021)

Enhancer of

Statement

Reference

Rab5^{S43N.UASp.YFP}, Scer\GAL4^c306 is an enhancer of abnormal cell migration | oogenesis phenotype of Scer\GAL4^c306, cactin^KK100507

(Miao et al., 2022)

Rab5^{S43N.UASp.YFP}/Scer\GAL4^GH146 is an enhancer | somatic clone of abnormal neuroanatomy | somatic clone phenotype of Strip^dogi

(Sakuma et al., 2014)

Scer\GAL4^nSyb.PS/Rab5^{S43N.UASp.YFP} is an enhancer of lethal phenotype of sky²/Df(2L)Exel7080

(Uytterhoeven et al., 2011)

Suppressor of

Statement

Reference

Rab5^{S43N.UASp.YFP}, Scer\GAL4^ninaE.PT is a suppressor of decreased cell number | adult stage | progressive phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

Rab5^{S43N.UASp.YFP}, Scer\GAL4^futsch-C380 is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of Scer\GAL4^futsch-C380, scat^HMS01910

(Patel et al., 2020)

Rab5^{S43N.UASp.YFP}, Scer\GAL4^Appl.G1a is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a

(Xu et al., 2016)

NOT Suppressor of

Statement

Reference

Scer\GAL4^GMR57C10/Rab5^{S43N.UASp.YFP} is a non-suppressor of bang sensitive phenotype of para^bss1

(Kroll et al., 2015)

Scer\GAL4^GMR57C10/Rab5^{S43N.UASp.YFP} is a non-suppressor of bang sensitive phenotype of eas²

(Kroll et al., 2015)

Rab5^{S43N.UASp.YFP}, Scer\GAL4^elav-C155 is a non-suppressor of abnormal neuroanatomy | embryonic stage phenotype of Scer\GAL4^elav-C155, comm^UAS.cKa

(van den Brink et al., 2013)

Other

Statement

Reference

Rab5^{S43N.UASp.YFP}, Scer\GAL4^αTub84B.PL, mtm^null has increased cell number | P-stage | somatic clone - tissue specific phenotype

(Hu et al., 2022)

Phenotype Manifest In

Enhancer of

Statement

Reference

Rab5^{S43N.UASp.YFP}, Scer\GAL4^c306 is an enhancer of border follicle cell phenotype of Scer\GAL4^c306, cactin^KK100507

(Miao et al., 2022)

Rab5^{S43N.UASp.YFP}/Scer\GAL4^GH146 is an enhancer | somatic clone of adult antennal lobe projection neuron DL1 adPN | somatic clone phenotype of Strip^dogi

(Sakuma et al., 2014)

NOT Enhancer of

Statement

Reference

Rab5^{S43N.UASp.YFP}, Scer\GAL4^55B is a non-enhancer of follicle cell phenotype of Scer\GAL4^55B, awd^RNAi.UAS

(Woolworth et al., 2009)

Suppressor of

Statement

Reference

Rab5^{S43N.UASp.YFP}, Scer\GAL4^ninaE.PT is a suppressor of outer photoreceptor cell | adult stage | decreased number phenotype of Hsap\BIN1^UAS.1, Scer\GAL4^ninaE.PT

(Lambert et al., 2022)

Rab5^{S43N.UASp.YFP}, Scer\GAL4^futsch-C380 is a suppressor | partially of embryonic/larval neuromuscular junction | larval stage phenotype of Scer\GAL4^futsch-C380, scat^HMS01910

(Patel et al., 2020)

Rab5^{S43N.UASp.YFP}, Scer\GAL4^futsch-C380 is a suppressor | partially of NMJ bouton | increased number | larval stage phenotype of Scer\GAL4^futsch-C380, scat^HMS01910

(Patel et al., 2020)

Scer\GAL4^ninaE.PU/Rab5^{S43N.UASp.YFP} is a suppressor of multivesicular body | pupal stage | conditional phenotype of PIP4K²⁹

(Kamalesh et al., 2017)

Scer\GAL4^ninaE.PU/Rab5^{S43N.UASp.YFP} is a suppressor of photoreceptor neuron | pupal stage | conditional phenotype of PIP4K²⁹

(Kamalesh et al., 2017)

Scer\GAL4^ninaE.PU/Rab5^{S43N.UASp.YFP} is a suppressor of vesicle | pupal stage | conditional phenotype of PIP4K²⁹

(Kamalesh et al., 2017)

Rab5^{S43N.UASp.YFP}, Scer\GAL4^Appl.G1a is a suppressor of larval segmental nerve | third instar larval stage phenotype of Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a

(Xu et al., 2016)

Rab5^{S43N.UASp.YFP}, Scer\GAL4^Appl.G1a is a suppressor of axon | third instar larval stage phenotype of Hsap\APP^695.UAS, Scer\GAL4^Appl.G1a

(Xu et al., 2016)

Scer\GAL4^Act5C.PI/Rab5^{S43N.UASp.YFP} is a suppressor of adherens junction | somatic clone phenotype of Rho1^72F

(Warner and Longmore, 2009)

Scer\GAL4^Act5C.PI/Rab5^{S43N.UASp.YFP} is a suppressor of adherens junction | somatic clone phenotype of Rho1^72O

(Warner and Longmore, 2009)

NOT Suppressor of

Statement

Reference

Rab5^{S43N.UASp.YFP}, Scer\GAL4^elav-C155 is a non-suppressor of larval longitudinal connective | embryonic stage phenotype of Scer\GAL4^elav-C155, comm^UAS.cKa

(van den Brink et al., 2013)

Rab5^{S43N.UASp.YFP}, Scer\GAL4^55B is a non-suppressor of follicle cell phenotype of Scer\GAL4^55B, awd^RNAi.UAS

(Woolworth et al., 2009)

Other

Statement

Reference

Rab5^{S43N.UASp.YFP}, Scer\GAL4^αTub84B.PL, mtm^null has interommatidial cell | increased number | P-stage | somatic clone - tissue specific phenotype

(Hu et al., 2022)

Rab5^{S43N.UASp.YFP}, Scer\GAL4^c306, cactin^GD9200 has egg chamber phenotype

(Miao et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

The expression of Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^ninaE.PU suppresses the expansion in endosome-like vesicles and multivesicular bodies observed in the photoreceptors of PIP4K²⁹ homozygous late pupae reared in light conditions.

(Kamalesh et al., 2017)

Expression of Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^GMR57C10 does not rescue the bang sensitivity of para^bss1/+ flies, hemizygous para^bss1 flies or eas² mutants.

(Kroll et al., 2015)

The axon elongation defect seen in Strip^dogi DL1 olfactory projection neuron single-cell clones is enhanced if the cells are also expressing Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP} (expression driven by Scer\GAL4^GH146).

(Sakuma et al., 2014)

Co-expression of Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP} has no effect on the "roundabout" phenotype (longitudinal axons crossing the midline) in embryos expressing comm^Scer\UAS.cKa under the control of Scer\GAL4^elav-C155.

(van den Brink et al., 2013)

Expression of Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^n-syb.PS enhances sky²/Df(2L)Exel7080 lethality; the double mutants die at the first larval instar stage.

(Uytterhoeven et al., 2011)

Expression of Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP} in Rho1^72F or Rho1^72O clones suppresses the adherens junction defect seen between two Rho1^72F or Rho1^72O cells, although decreased apical tension is not affected.

(Warner and Longmore, 2009)

The follicle cell epithelial defects seen in females expressing awd^{dsRNA.Scer\UAS} under the control of Scer\GAL4^55B are not rescued or exacerbated by co-expression of Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP}.

(Woolworth et al., 2009)

Xenogenetic Interactions

Statement

Reference

The presence of axonal blockages in the segmental nerves characteristic for third instar larvae expressing Hsap\APP^695.Scer\UAS under the control of Scer\GAL4^Appl.G1a is rescued by co-expression of Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP}.

(Xu et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

9771

y¹ w^*; P{UASp-YFP.Rab5.S43N}01

9772

y¹ w^*; P{UASp-YFP.Rab5.S43N}Eip75B⁰²/TM3, Sb¹

Notes on Origin

Discoverer

Comments

Carried in a plasmid and transfected into S2R+ cells.

(Zhang et al., 2007)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Rab5^DN

(Lambert et al., 2022, Kamalesh et al., 2017, Sahai-Hernandez and Nystul, 2013)

Rab5^{S43N.Scer\UAS.P\T.T:Avic\GFP-YFP}

Rab5^{S43N.UASp.YFP}

Rab5^S43N

(Woolworth et al., 2009)

Rab5DN

(Sakuma et al., 2014)

Rab5S43N

(Sakuma et al., 2014)

rab5^DN

(Uytterhoeven et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (51)

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