FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\cacGD3326
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General Information
Symbol
Dmel\cacGD3326
Species
D. melanogaster
Name
FlyBase ID
FBal0209212
Feature type
allele
Associated gene
Dmel\cac
Associated Insertion(s)
Carried in Construct
P{GD3326}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Dickson et al., 2007.7.18)
Mutagen
in vitro construct
(Dickson et al., 2007.7.18)
Progenitor genotype
Carried in construct
P{GD3326}
Cytology
Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
dsRNA-GD3326
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of cacGD3326 in the adult MN5 neuron under the control of Scer\GAL4D42 (combined with Scer\GAL80Cha.PK to suppress expression in cholinergic interneurons and Dcr-1GD11429 to enhance the RNAi efficiency) results in calcium current phenotypes. The amplitude of the transient low voltage activated (LVA) calcium current is reduced by 70-80% compared to wild type and all high voltage amplitude (HVA) activated calcium currents in the MN5 neuron are abolished. Consequently, the total calcium current (LVA and fast HVA) is reduced by more than 90% compared to wild type.

      (Ryglewski et al., 2012)

      Expression of cacGD3326 (which affects the α1 subunit of cac) under the control of Scer\GAL4elav-C155, results in a 50% reduction of EPSC amplitude. Sustained release is unaltered in cacGD3326 mutants.

      (Hallermann et al., 2010)

      Expression of cacGD3326 under the control of Scer\GAL4elav.PLu results in flies with abnormal wing posture and defective flight behavior.

      (Dietzl et al., 2007)
      External Data
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      cacGD3326
      Name Synonyms
      Secondary FlyBase IDs
        References (10)