FlyBase Allele Report: Dmel\Ctr1B[3-4]

General Information

Symbol

Dmel\Ctr1B^3-4

Species

D. melanogaster

Name

FlyBase ID

FBal0156757

Feature type

allele

Associated gene

Dmel\Ctr1B

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Zhou et al., 2003)

Progenitor genotype

P{EP}EP833

(Zhou et al., 2003)

Cytology

Description

Imprecise excision of the P{EP}EP833 element, resulting in a deletion of about 1.2kb of genomic sequence, which removes the entire first exon of Ctr1B (including the transcription start site and ATG codon).

(Zhou et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color | conditional

(Zhou et al., 2003)

chemical sensitive

(Zhou et al., 2003)

lethal | conditional (with Df(3R)p40)

(Zhou et al., 2003)

lethal | conditional | recessive

(Zhou et al., 2003)

male fertile

(Steiger et al., 2010)

Phenotype Manifest In

adult cuticle | conditional

(Zhou et al., 2003)

Detailed Description

Statement

Reference

Ctr1B^3-4 mutant males are fertile.

(Steiger et al., 2010)

Ctr1B^3-4 mutants develop well in normal food (Cu content, 2-5υM), but are sensitive to copper depletion. These mutants are also sensitive to non-essential metals such as mercury and cadmium, but not zinc. Supplementation with copper largely rescues the lethality associated with 250υM of either mercury or cadmium-infused food. By contrast, food supplementation with zinc fails to alleviate the deleterious effects of mercury or cadmium.

(Balamurugan et al., 2009)

Homozygous larvae accumulate about 40% of the copper accumulated by wild-type larvae when raised on standard food. When raised under conditions of copper limitation, wild-type larvae accumulate nearly 4 times the total copper as homozygous larvae. The frequency of development to adulthood decreases dramatically for homozygous flies as the availability of copper in the food is decreased by addition of a copper chelator; no adult homozygotes are recovered at a concentration of chelator at which wild-type flies are recovered at a frequency comparable to that on normal food. This arrest of development is also seen in Ctr1B^3-4/Df(3R)p40 animals. The homozygotes raised on limited copper are arrested as second instar larvae and fail to burrow into the food, in contrast to what is seen for wild-type larvae or for homozygous larvae raised on standard food. This developmental defect seen when homozygotes are raised on food containing a copper chelator can be partially rescued by the addition of copper to the food. Heterozygous flies have normal viability, morphology and fertility after being raised for three generations on food containing a copper chelator. However, if the larvae are raised on food containing both a copper chelator and 100μM AgNO₃ (which is predicted to severely reduce dietary copper availability), dramatic defects are seen in the heterozygous adults (but not in wild-type adults raised on the same medium). The heterozygotes have pigmentation defects after emergence from the pupal case, with the most obvious defects in the abdominal region. However, within a day of eclosion, the pigmentation of the flies is virtually indistinguishable from that of wild-type flies. Homozygous larvae show a dramatically lower tolerance to elevated levels of copper in the food compared to wild-type larvae. Most of the homozygous larvae survive through pupation and arrest at a late pupal stage with visible folded wings.

(Zhou et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ctr1B^3-4, Ctr1C^6D has male semi-sterile phenotype, suppressible by Ctr1C^EGFP

(Steiger et al., 2010)

Ctr1B^3-4, Ctr1C^6D has male semi-sterile phenotype, suppressible by Ctr1C^+t8

(Steiger et al., 2010)

Ctr1B^3-4, Ctr1C^6D has male semi-sterile phenotype, suppressible by Ctr1B^GFP

(Steiger et al., 2010)

Ctr1B^3-4 has male fertile phenotype, suppressible by Ctr1C^6D

(Steiger et al., 2010)

Other

Statement

Reference

Ctr1B^3-4, Ctr1C^6D has male semi-sterile phenotype

(Steiger et al., 2010)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Although 12 day old mutant Ctr1B^3-4 males are fertile, Ctr1B^3-4 Ctr1C^6D double mutant males display almost complete sterility, indicating that in the absence of Ctr1B, Ctr1C is required for male fertility. These males are only partially sterile before this time however. Testes at any age do not reveal obvious morphological differences compared to control males. If Ctr1B^3-4 Ctr1C^6D double mutant males are kept on food supplemented with copper after eclosion, i.e. as adult flies, for 12 days, fertility is largely restored. Fertility is also restored by the presence of Ctr1C^{T:Avic\GFP-EGFP}, Ctr1C^+t8 or Ctr1B^T:Avic\GFP.

(Steiger et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Ctr1B^3-4 is rescued by Ctr1B^AH3.EGFP

(Selvaraj et al., 2005)

Not rescued by

Ctr1B^3-4 is not rescued by Ctr1B^{m1-m2-3-4.EGFP}

(Selvaraj et al., 2005)

Ctr1B^3-4 is not rescued by Ctr1B^{m1-m2-m3-m4.EGFP}

(Selvaraj et al., 2005)

Comments

Fertility in Ctr1B^3-4 Ctr1C^6D double mutant males is restored by the presence of Ctr1B^T:Avic\GFP.

Fertility in Ctr1B^3-4 Ctr1C^6D double mutant males is restored by the presence of Ctr1C^{T:Avic\GFP-EGFP} or Ctr1C^+t8.

(Steiger et al., 2010)

The presence of Ctr1B^{AH3.T:Avic\GFP-EGFP} in a Ctr1B^3-4 mutant background rescues the lethality in a low-copper environment. Ctr1B^{m1-m2-3-4.T:Avic\GFP-EGFP} or Ctr1B^{m1-m2-m3-m4.T:Avic\GFP-EGFP} do not rescue the low copper lethality phenotype.

(Selvaraj et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

29019

w¹¹¹⁸; Ctr1B^3-4/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ctr1B^3-4

(Steiger et al., 2010)

ctr1B^3-4

(Zhou et al., 2003)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

Open Close