FlyBase Allele Report: Dmel\rno[1]

General Information

Symbol

Dmel\rno¹

Species

D. melanogaster

Name

FlyBase ID

FBal0154696

Feature type

allele

Associated gene

Dmel\rno

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Voas and Rebay, 2003)

Progenitor genotype

Cytology

Description

Amino acid replacement: W292term.

(Voas and Rebay, 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:197,527..197,527 [-]

Nucleotide change:

G197527A

Amino acid change:

W292term | rno-PB; W292term | rno-PC; W292term | rno-PD

Reported amino acid change:

W292term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Voas and Rebay, 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | somatic clone | third instar larval stage

(Steele et al., 2009)

lethal (with Df(3L)XKR845)

(Voas and Rebay, 2003)

lethal (with Df(3L)XZB970)

(Voas and Rebay, 2003)

lethal | recessive

(Voas and Rebay, 2003)

Phenotype Manifest In

photoreceptor cell R8 | somatic clone | third instar larval stage

(Steele et al., 2009)

photoreceptor neuron | somatic clone | third instar larval stage

(Steele et al., 2009)

Detailed Description

Statement

Reference

rno¹ somatic clones generated during eye development result in mostly normal adult eye morphology.

Occasionally (in less than 5% of cases) rno¹ third instar larval eye disc clones develop multiple photoreceptor R8 cells within a single ommatidial cluster.

In third instar larval eye discs, cells from somatic clones mutant for rno¹ show slight delays in photoreceptor differentiation compared to surrounding control tissue.

(Steele et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rbf^15aΔ, rno¹ has increased cell number | third instar larval stage | somatic clone phenotype, enhanceable by Delta⁰⁵¹⁵¹/Dl[+]

(Steele et al., 2009)

rno¹ has increased cell number | third instar larval stage | somatic clone phenotype, enhanceable by Rbf^15aΔ

(Steele et al., 2009)

rno¹ has increased cell number | third instar larval stage | somatic clone phenotype, enhanceable by Delta⁰⁵¹⁵¹/Dl[+]

(Steele et al., 2009)

NOT Enhanced by

Statement

Reference

rno¹ has increased cell number | third instar larval stage | somatic clone phenotype, non-enhanceable by Egfr[+]/Egfr^f2

(Steele et al., 2009)

Rbf^15aΔ, rno¹ has increased cell number | third instar larval stage | somatic clone phenotype, non-enhanceable by Egfr[+]/Egfr^f2

(Steele et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

rno¹ has photoreceptor cell R8 | third instar larval stage | somatic clone phenotype, enhanceable by Rbf^15aΔ

(Steele et al., 2009)

rno¹ has photoreceptor neuron | third instar larval stage | somatic clone phenotype, enhanceable by Rbf^15aΔ

(Steele et al., 2009)

rno¹ has photoreceptor cell R8 | third instar larval stage | somatic clone phenotype, enhanceable by Delta⁰⁵¹⁵¹/Dl[+]

(Steele et al., 2009)

Rbf^15aΔ, rno¹ has photoreceptor cell R8 | third instar larval stage | somatic clone phenotype, enhanceable by Delta⁰⁵¹⁵¹/Dl[+]

(Steele et al., 2009)

NOT Enhanced by

Statement

Reference

rno¹ has photoreceptor cell R8 | third instar larval stage | somatic clone phenotype, non-enhanceable by E2f1^su89

(Steele et al., 2009)

rno¹ has photoreceptor cell R8 | third instar larval stage | somatic clone phenotype, non-enhanceable by Egfr[+]/Egfr^f2

(Steele et al., 2009)

Rbf^15aΔ, rno¹ has photoreceptor cell R8 | third instar larval stage | somatic clone phenotype, non-enhanceable by Egfr[+]/Egfr^f2

(Steele et al., 2009)

Suppressed by

Statement

Reference

Rbf^15aΔ, rno¹ has eye | somatic clone phenotype, suppressible by E2f1ⁱ²/E2f1^rM729

(Steele et al., 2009)

Rbf^15aΔ, rno¹ has photoreceptor cell R8 | somatic clone | third instar larval stage phenotype, suppressible by E2f1ⁱ²/E2f1^rM729

(Steele et al., 2009)

Other

Statement

Reference

Rbf^15aΔ, rno¹ has eye | somatic clone phenotype

(Steele et al., 2009)

Rbf^15aΔ, rno¹ has interommatidial bristle | somatic clone phenotype

(Steele et al., 2009)

Rbf^15aΔ, rno¹ has photoreceptor cell R8 | somatic clone | third instar larval stage phenotype

(Steele et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Doubly mutant rno¹ and Rbf^15aΔ somatic clones generated during eye development result in shiny eye differentiation defects in the adult eye. Pupal eye discs are largely devoid of bristle cells, and adult eyes lack bristles.

Multiple photoreceptor R8 cells are observed within the same ommatidial cluster in roughly 20% of third instar larval eye disc clones mutant for both rno¹ and Rbf^15aΔ.

The multiple-R8 phenotype seen in rno¹ or rno¹, Rbf^15aΔ eye somatic mutant clones is not significantly affected by Egfr^f2/+, but is significantly enhanced in a Dl⁰⁵¹⁵¹/+ background.

The shiny-eye and multiple photoreceptor-R8 phenotype seen in rno¹, Rbf^15aΔ eye somatic mutant clones is significantly suppressed in a E2fⁱ²/E2f^rM729 mutant background.

In third instar larval eye discs, cells from somatic clones mutant for both rno¹ and Rbf^15aΔ show significant and enhanced delays in photoreceptor differentiation compared to controls.

The shiny-eye phenotype and multiple photoreceptor-R8 phenotype observed in rno¹ somatic clones in the eye is not enhanced in a E2f^su89 background.

(Steele et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

rno^K

(Steele et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

rno¹

(Steele et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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