FlyBase Allele Report: Dmel\14-3-3ε[UAS.cCa]

General Information

Symbol

Dmel\14-3-3ε^UAS.cCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Chen

FlyBase ID

FBal0148515

Feature type

allele

Associated gene

Dmel\14-3-3ε

Associated Insertion(s)

Carried in Construct

P{UAS-14-3-3ε.C}

Also Known As

UAS-d14-3-3ε

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Chen et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-14-3-3ε.C}

Cytology

Description

UASt sequences drive expression of 14-3-3ε.

(Chen et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

14-3-3ε

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Branco et al., 2008, Kaltenbach et al., 2007)

exacerbates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN1^82Q.UAS

(Chen et al., 2003)

exacerbates olivopontocerebellar atrophy

modeled by Hsap\ATXN1^82Q.UAS

(Branco et al., 2008)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

No retinal phenotypes are seen when 14-3-3ε^Scer\UAS.cCa is expressed under the control of Scer\GAL4^GMR.PU.

(Branco et al., 2008)

Overexpression of 14-3-3ε^Scer\UAS.cCa driven by Scer\GAL4[GMR.PF] in the retina does not affect eye structure.

(Nielsen et al., 2008)

When 14-3-3ε^Scer\UAS.cCa is driven by Scer\GAL4^GMR.PF no deleterious effects are seen.

(Chen et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

14-3-3ε^UAS.cCa, Scer\GAL4^GMR.PU is an enhancer of visible phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Kaltenbach et al., 2007)

Suppressor of

Statement

Reference

14-3-3ε^UAS.cCa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Scer\GAL4^GMR.PF, foxo^UAS.Tag:FLAG

(Nielsen et al., 2008)

14-3-3ε^UAS.cCa, Scer\GAL4^Ilp2.PR is a suppressor of abnormal body size phenotype of Scer\GAL4^Ilp2.PR, foxo^UAS.Tag:FLAG

(Nielsen et al., 2008)

14-3-3ε^UAS.cCa, Scer\GAL4^ppl.PP is a suppressor of abnormal body size phenotype of Scer\GAL4^ppl.PP, foxo^UAS.Tag:FLAG

(Nielsen et al., 2008)

Other

Statement

Reference

14-3-3ε^UAS.cCa, Hsap\ATXN1^82Q.UAS has lethal phenotype

(Chen et al., 2003)

14-3-3ε^UAS.cCa, Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF has lethal phenotype

(Chen et al., 2003)

Phenotype Manifest In

Enhancer of

Statement

Reference

14-3-3ε^UAS.cCa, Scer\GAL4^GMR.PU is an enhancer of retina phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Branco et al., 2008)

14-3-3ε^UAS.cCa, Scer\GAL4^GMR.PU is an enhancer of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PU

(Branco et al., 2008)

14-3-3ε^UAS.cCa, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Kaltenbach et al., 2007)

14-3-3ε^UAS.cCa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Chen et al., 2003)

14-3-3ε^UAS.cCa, Scer\GAL4^GMR.PF is an enhancer of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Chen et al., 2003)

14-3-3ε^UAS.cCa, Scer\GAL4^GMR.PF is an enhancer of rhabdomere phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PF

(Chen et al., 2003)

Suppressor of

Statement

Reference

14-3-3ε^UAS.cCa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, foxo^UAS.Tag:FLAG

(Nielsen et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

The eye phenotype resulting from the overexpression of foxo^{Scer\UAS.T:Zzzz\FLAG} driven by Scer\GAL4^GMR.PF is suppressed by 14-3-3ε^Scer\UAS.cCa overexpression.

The Scer\GAL4^Ilp2.PR-driven foxo^{Scer\UAS.T:Zzzz\FLAG}-overexpression induced small body size phenotype is suppressed by the co-overexpression of 14-3-3ε^Scer\UAS.cCa.

The Scer\GAL4^ppl.PP-driven foxo^{Scer\UAS.T:Zzzz\FLAG}-overexpression induced small body size phenotype is suppressed by the co-overexpression of 14-3-3ε^Scer\UAS.cCa.

(Nielsen et al., 2008)

Xenogenetic Interactions

Statement

Reference

Expression of 14-3-3ε^Scer\UAS.cCa enhances the retinal phenotype seen when Hsap\HTT^{128Q.1-336.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PU.

Expression of 14-3-3ε^Scer\UAS.cCa enhances the retinal phenotype seen when Hsap\ATXN1^82Q.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PU.

(Branco et al., 2008)

The addition of 14-3-3ε^Scer\UAS.cCa enhances the eye phenotypes seen in Hsap\ATX1^82Q.Scer\UAS, Scer\GAL4^GMR.PF animals alone. They have profoundly disordered ommatidia, a thin and disorganised retina layer, and grossly abnormal rhabdomeres.

(Chen et al., 2003)

Complementation and Rescue Data

Partially rescues

14-3-3ε^UAS.cCa/Scer\GAL4^αTub84B.PL partially rescues 14-3-3ε^ex4

(Tsigkari et al., 2012)

Fails to rescue

14-3-3ε^UAS.cCa/Scer\GAL4^Act5C.PI fails to rescue 14-3-3ε^ex4

(Tsigkari et al., 2012)

Comments

The reduction in the number of pole cells per gonad seen in 14-3-3ε^ex4 embryos is almost completely rescued by expression of 14-3-3ε^Scer\UAS.cCa under the control of Scer\GAL4^αTub84B.PL but not under the control of Scer\GAL4^Act5C.PI.

(Tsigkari et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

14-3-3ε^Scer\UAS.cCa

14-3-3ε^UAS.cCa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Chen

(Chen et al., 2003)

Secondary FlyBase IDs

References (5)

Report Sections

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